RLG00000004222

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012613
Physical Location & Seq
Reverse (-)
57363845 .. 57366467
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004222

Sequence Viewer

Length: 1068 bp
ATGGAGACTTATCTGCCATTTGCAGGGATGGTAATGGTGGAGTGCCTGGATGTGGGAATGACCACACTAAGCAAAGCGGCCATGTCCAGAGGAATGAGTCACTTTGTTTTTGTTGTCTACTCAAATGCTCTTGCCACTCTCATCCTCCTCCCTTCTTCTTTCATAATCCACAGAAATAAGAGACCCCCATTGACCTTCTCCATTCTCTGTAAATTCTTCCTTCTTAGCCTTGCTGGCTTTTACACCATTAGACATGGAGGGCTTCTTACCCTTGTTTTGTTGACTTTACCTCAAATTTTCAGGATAACTATTATGCAGAACTGTGTAATGGAAAAGTTAGATTTGAGAAACTCAATAAGCCAGATCAAAATCATGGGCACCCTGGTATCTATATCAGGAGCATTGATTGTCACTCTCTACAAGGGTGCTCCACTTTTTGCCACACCAGTTCAATCAATTCCTGACTCTTCTCCCTCGAACACCTCTTCATCGACATCAATGAATAATTGGATCATTGGAGGCCTTTTTCTTGCAACAGCTTCTTCATGTCTTGCAATATGGAATACTGCTCAGGCTGCAATTCTTAAAGGATACCCCTCAGAGATGACCATAGTCTCCTTCTATTCCTTATTTGGAACCATTCAGTGTACAATACTTTCTTTGCTTGTTGAAAGGAATCCAAGTGCCTGGACACTGAGGCCTGACATTGAGCTTGCCTCTATCTTCTATTCAGCTATTTGTGGAGGTGTTGTAACATTCTCTGTGCAAACATGGTGCATAAAAAAGAAAGGACCTGTTTTTGTGGCTATGTTCAAGCCTTTGGGGATTGCCATTGCTGCTTTGCTGGGTGCTATCTTTCTAGGGGAAGAACTCCATATTGGAAGCCTTATTGGAGCAGTGGTAATTGTTGTGGGATTTTACGGAGTAATCTGGGCGCAATCAAAAGAGGAAAAAGGTTTGGATATAAAAACAAAGGAAAATGGTGAATCCCAGGAGGATGATGAGATTGATAGGCTGCAGTCGTCATCTCAGAAGAAGCCTCTATTGGAGAGCGACGTACATGTCTAA

Protein Analysis

356

Amino Acids

38.65

Weight (kDa)

8.11

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 172 - 310 6.7e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1061
AccB1I GGYRCC 1 cut(s) 377
AccI GTMKAC 1 cut(s) 117
AciI CCGC 1 cut(s) 77
AclWI GGATC 1 cut(s) 518
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 2 cut(s) 212, 294
AfaI GTAC 2 cut(s) 649, 1059
AfiI CCNNNNNNNGG 2 cut(s) 23, 52
AflIII ACRYGT 1 cut(s) 1060
AgsI TTSAA 3 cut(s) 452, 671, 814
AjnI CCWGG 4 cut(s) 45, 381, 686, 990
AjuI GAANNNNNNNTTGG 4 cut(s) 861, 893, 1028, 1060
AluBI AGCT 3 cut(s) 539, 712, 734
AluI AGCT 3 cut(s) 539, 712, 734
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 2 cut(s) 175, 619
AlwI GGATC 1 cut(s) 518
AoxI GGCC 3 cut(s) 78, 520, 698
ApeKI GCWGC 3 cut(s) 575, 836, 1015
ApoI RAATTY 2 cut(s) 212, 294
AspLEI GCGC 1 cut(s) 937
AspS9I GGNCC 1 cut(s) 791
AsuHPI GGTGA 1 cut(s) 995
AvaII GGWCC 1 cut(s) 791
BaeGI GKGCMC 1 cut(s) 380
BaeI ACNNNNGTAYC 2 cut(s) 369, 402
BanI GGYRCC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 3 cut(s) 562, 823, 1002
BccI CCATC 1 cut(s) 22
BciT130I CCWGG 4 cut(s) 47, 383, 688, 992
BciVI GTATCC 1 cut(s) 584
BcoDI GTCTC 2 cut(s) 175, 619
BfaI CTAG 1 cut(s) 860
BfmI CTRYAG 1 cut(s) 1016
BfuI GTATCC 1 cut(s) 584
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 4 cut(s) 78, 576, 837, 1016
BlsI GCNGC 4 cut(s) 79, 577, 838, 1017
Bme1390I CCNGG 4 cut(s) 47, 383, 688, 992
Bme18I GGWCC 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 791
BmiI GGNNCC 2 cut(s) 379, 637
BmrFI CCNGG 4 cut(s) 47, 383, 688, 992
BoxI GACNNNNGTC 1 cut(s) 611
BplI GAGNNNNNCTC 2 cut(s) 701, 733
Bpu10I CCTNAGC 1 cut(s) 570
BsaBI GATNNNNATC 1 cut(s) 368
BsaI GGTCTC 1 cut(s) 175
BsaJI CCNNGG 2 cut(s) 381, 990
BsaXI ACNNNNNCTCC 4 cut(s) 735, 765, 885, 915
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 52
Bse1I ACTGG 1 cut(s) 446
Bse3DI GCAATG 1 cut(s) 831
Bse8I GATNNNNATC 1 cut(s) 368
BseBI CCWGG 4 cut(s) 47, 383, 688, 992
BseDI CCNNGG 2 cut(s) 381, 990
BseGI GGATG 4 cut(s) 33, 55, 141, 1003
BseJI GATNNNNATC 1 cut(s) 368
BseLI CCNNNNNNNGG 2 cut(s) 23, 52
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 4 cut(s) 584, 612, 686, 1043
BseNI ACTGG 1 cut(s) 446
BseRI GAGGAG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 380
BseXI GCAGC 3 cut(s) 562, 823, 1002
BseYI CCCAGC 1 cut(s) 844
BshFI GGCC 3 cut(s) 80, 522, 700
BshNI GGYRCC 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 430
BslI CCNNNNNNNGG 2 cut(s) 23, 52
BsmAI GTCTC 2 cut(s) 175, 619
BsnI GGCC 3 cut(s) 80, 522, 700
Bso31I GGTCTC 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 380, 430
Bsp1407I TGTACA 1 cut(s) 647
Bsp143I GATC 2 cut(s) 363, 510
BspACI CCGC 1 cut(s) 77
BspANI GGCC 3 cut(s) 80, 522, 700
BspCNI CTCAG 4 cut(s) 583, 611, 687, 1042
BspLI GGNNCC 2 cut(s) 379, 637
BspMAI CTGCAG 1 cut(s) 1020
BspPI GGATC 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 175
BsrDI GCAATG 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 647
BsrI ACTGG 1 cut(s) 446
BssECI CCNNGG 2 cut(s) 381, 990
BssMI GATC 2 cut(s) 363, 510
Bst2UI CCWGG 4 cut(s) 47, 383, 688, 992
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 472, 490
BstAUI TGTACA 1 cut(s) 647
BstC8I GCNNGC 2 cut(s) 235, 714
BstDEI CTNAG 6 cut(s) 68, 224, 570, 598, 695, 1029
BstF5I GGATG 4 cut(s) 33, 55, 141, 1003
BstHHI GCGC 1 cut(s) 937
BstKTI GATC 2 cut(s) 366, 513
BstMAI GTCTC 2 cut(s) 175, 619
BstMBI GATC 2 cut(s) 363, 510
BstMWI GCNNNNNNNGC 3 cut(s) 234, 575, 836
BstNI CCWGG 4 cut(s) 47, 383, 688, 992
BstNSI RCATGY 1 cut(s) 1064
BstPAI GACNNNNGTC 1 cut(s) 611
BstSCI CCNGG 4 cut(s) 45, 381, 686, 990
BstSFI CTRYAG 1 cut(s) 1016
BstSLI GKGCMC 1 cut(s) 380
BstV1I GCAGC 3 cut(s) 562, 823, 1002
BstXI CCANNNNNNTGG 1 cut(s) 687
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 3 cut(s) 80, 522, 700
BtsCI GGATG 4 cut(s) 33, 55, 141, 1003
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 3 cut(s) 650, 692, 903
Cac8I GCNNGC 2 cut(s) 235, 714
CfoI GCGC 1 cut(s) 937
Cfr13I GGNCC 1 cut(s) 791
Csp6I GTAC 2 cut(s) 648, 1058
CviAII CATG 6 cut(s) 82, 254, 373, 546, 771, 1061
CviQI GTAC 2 cut(s) 648, 1058
DdeI CTNAG 6 cut(s) 68, 224, 570, 598, 695, 1029
DpnI GATC 2 cut(s) 365, 512
DpnII GATC 2 cut(s) 363, 510
DrdI GACNNNNNNGTC 1 cut(s) 1061
DseDI GACNNNNNNGTC 1 cut(s) 1061
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 472, 490
EarI CTCTTC 2 cut(s) 472, 490
Eco147I AGGCCT 2 cut(s) 522, 700
Eco31I GGTCTC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 791
EcoO109I RGGNCCY 1 cut(s) 791
EcoRII CCWGG 4 cut(s) 45, 381, 686, 990
FaeI CATG 6 cut(s) 85, 257, 376, 549, 774, 1064
FatI CATG 6 cut(s) 81, 253, 372, 545, 770, 1060
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 4 cut(s) 78, 576, 837, 1016
FokI GGATG 4 cut(s) 40, 62, 128, 1010
Fsp4HI GCNGC 4 cut(s) 78, 576, 837, 1016
FspBI CTAG 1 cut(s) 860
GlaI GCGC 1 cut(s) 936
GluI GCNGC 4 cut(s) 78, 576, 837, 1016
GsaI CCCAGC 1 cut(s) 848
HaeIII GGCC 3 cut(s) 80, 522, 700
HhaI GCGC 1 cut(s) 937
Hin1II CATG 6 cut(s) 85, 257, 376, 549, 774, 1064
Hin6I GCGC 1 cut(s) 935
HinP1I GCGC 1 cut(s) 935
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 4 cut(s) 97, 464, 676, 986
HphI GGTGA 1 cut(s) 995
Hpy166II GTNNAC 3 cut(s) 118, 282, 648
Hpy188I TCNGA 2 cut(s) 601, 1032
Hpy188III TCNNGA 4 cut(s) 87, 301, 396, 461
Hpy8I GTNNAC 3 cut(s) 118, 282, 648
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 4 cut(s) 162, 205, 230, 628
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 1 cut(s) 1056
HpyCH4V TGCA 8 cut(s) 23, 316, 533, 554, 578, 766, 777, 1018
HpyF10VI GCNNNNNNNGC 3 cut(s) 234, 575, 836
HpyF3I CTNAG 6 cut(s) 68, 224, 570, 598, 695, 1029
HpySE526I ACGT 1 cut(s) 1056
Hsp92II CATG 6 cut(s) 85, 257, 376, 549, 774, 1064
HspAI GCGC 1 cut(s) 935
Kzo9I GATC 2 cut(s) 363, 510
LmnI GCTCC 3 cut(s) 398, 433, 893
Lsp1109I GCAGC 3 cut(s) 562, 823, 1002
MaeI CTAG 1 cut(s) 860
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 3 cut(s) 98, 409, 751
MalI GATC 2 cut(s) 365, 512
MboI GATC 2 cut(s) 363, 510
MboII GAAGA 8 cut(s) 147, 208, 459, 477, 534, 715, 878, 1045
MhlI GDGCHC 2 cut(s) 380, 430
MluCI AATT 6 cut(s) 212, 294, 456, 505, 579, 903
MlyI GAGTC 2 cut(s) 106, 458
MseI TTAA 1 cut(s) 585
MspR9I CCNGG 4 cut(s) 47, 383, 688, 992
MvaI CCWGG 4 cut(s) 47, 383, 688, 992
MwoI GCNNNNNNNGC 3 cut(s) 234, 575, 836
NdeII GATC 2 cut(s) 363, 510
NlaIII CATG 6 cut(s) 85, 257, 376, 549, 774, 1064
NlaIV GGNNCC 2 cut(s) 379, 637
NmuCI GTSAC 2 cut(s) 98, 409
NspI RCATGY 1 cut(s) 1064
PceI AGGCCT 2 cut(s) 522, 700
PciI ACATGT 1 cut(s) 1060
PfeI GAWTC 2 cut(s) 676, 986
PkrI GCNGC 4 cut(s) 79, 577, 838, 1017
PleI GAGTC 2 cut(s) 105, 458
PpsI GAGTC 2 cut(s) 105, 458
PpuMI RGGWCCY 1 cut(s) 791
PscI ACATGT 1 cut(s) 1060
PshAI GACNNNNGTC 1 cut(s) 611
Psp5II RGGWCCY 1 cut(s) 791
Psp6I CCWGG 4 cut(s) 45, 381, 686, 990
PspFI CCCAGC 1 cut(s) 844
PspGI CCWGG 4 cut(s) 45, 381, 686, 990
PspN4I GGNNCC 2 cut(s) 379, 637
PspPI GGNCC 1 cut(s) 791
PspPPI RGGWCCY 1 cut(s) 791
PstI CTGCAG 1 cut(s) 1020
RsaI GTAC 2 cut(s) 649, 1059
RsaNI GTAC 2 cut(s) 648, 1058
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 4 cut(s) 78, 576, 837, 1016
Sau3AI GATC 2 cut(s) 363, 510
Sau96I GGNCC 1 cut(s) 791
SchI GAGTC 2 cut(s) 106, 458
ScrFI CCNGG 4 cut(s) 47, 383, 688, 992
SduI GDGCHC 2 cut(s) 380, 430
SfcI CTRYAG 1 cut(s) 1016
SinI GGWCC 1 cut(s) 791
Sse9I AATT 6 cut(s) 212, 294, 456, 505, 579, 903
SseBI AGGCCT 2 cut(s) 522, 700
SsiI CCGC 1 cut(s) 77
SspMI CTAG 1 cut(s) 860
StuI AGGCCT 2 cut(s) 522, 700
StyD4I CCNGG 4 cut(s) 45, 381, 686, 990
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 2 cut(s) 476, 491
TasI AATT 6 cut(s) 212, 294, 456, 505, 579, 903
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 2 cut(s) 676, 986
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 3 cut(s) 650, 699, 903
TseFI GTSAC 2 cut(s) 98, 409
TseI GCWGC 3 cut(s) 575, 836, 1015
Tsp45I GTSAC 2 cut(s) 98, 409
TspDTI ATGAA 4 cut(s) 151, 477, 515, 534
TspGWI ACGGA 1 cut(s) 936
TspRI CASTG 3 cut(s) 650, 699, 903
VpaK11BI GGWCC 1 cut(s) 791
XapI RAATTY 2 cut(s) 212, 294
XceI RCATGY 1 cut(s) 1064
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 1 cut(s) 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.