Rh7BG141200

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012613
Physical Location & Seq
Forward (+)
10464096 .. 10465882
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG141200.1

Sequence Viewer

Length: 696 bp
ATGGGCACCCTGGTATCTATATCAGGAGCATTGATTGTCACTCTCTACAAGGGTGCTCCACTTTTAGCCACACCAGCTCAATCAATTCCTGACTCTTCTCCCTCAAACACCTCTTCGTCGACATCAATGAATAATTGGATCATTGGAGGTCTTTTTCTTGCAACAGCTTCTTCATGTCTTGCAATATGGAATACTGCTCAGGCTGCAATTCTTAAAGGATACCCCTCAGAGATGACCATAGTGTCCTTCTATTCCTTATTTGGAACCATTCAGTGTACAATACTTTCTTTGTTTGTTGAAAGGAATCCAAGTGCCTGGACACTGAGGCCTGACATTGAGCTTGCCTCTATCTTCTATTCAGCTATTTGTGGAGGTGTTGTAACATTCTCTGTGCAAACATGGTGCATAAAAAAGAAAGGACCTGTATTTGTGGCTATGTTCAAGCCTTTGGGGATTGCCATTGCTGCTTTGCTGGGTGCTATCTTTCTAGGGGAAGAACTCCATGTTGGAAGCCTTATTGGAGCAGTGGTAATTGTTGTGGGATTTTACGGAGTAATCTGGGCGCAATCAAAAGAGGAAAAAGGTTTGGATATAAAAACAAAGGAAGATGGTGAATCCCAGGAGGATGATGAGATTGATAGGCTGCAGTCGTCATCTCAGAAGAAGCCTCTATTGGAGAGCGCAGTACATGTTTAA

Protein Analysis

231

Amino Acids

24.61

Weight (kDa)

5.2

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 48 - 186 1.2e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 241
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 119
AclWI GGATC 1 cut(s) 146
AfaI GTAC 2 cut(s) 277, 687
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 2 cut(s) 299, 442
AjnI CCWGG 3 cut(s) 9, 314, 618
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 656, 688
AluBI AGCT 4 cut(s) 77, 167, 340, 362
AluI AGCT 4 cut(s) 77, 167, 340, 362
Alw21I GWGCWC 1 cut(s) 58
AlwI GGATC 1 cut(s) 146
AoxI GGCC 1 cut(s) 326
ApeKI GCWGC 3 cut(s) 203, 464, 643
AspLEI GCGC 2 cut(s) 565, 683
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 1 cut(s) 623
AvaII GGWCC 1 cut(s) 419
BaeGI GKGCMC 1 cut(s) 8
BaeI ACNNNNGTAYC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 3 cut(s) 190, 451, 630
BccI CCATC 1 cut(s) 602
BciT130I CCWGG 3 cut(s) 11, 316, 620
BciVI GTATCC 1 cut(s) 212
BfaI CTAG 1 cut(s) 488
BfmI CTRYAG 1 cut(s) 644
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 3 cut(s) 204, 465, 644
BlsI GCNGC 3 cut(s) 205, 466, 645
Bme1390I CCNGG 3 cut(s) 11, 316, 620
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 2 cut(s) 7, 265
BmrFI CCNGG 3 cut(s) 11, 316, 620
BplI GAGNNNNNCTC 2 cut(s) 329, 361
Bpu10I CCTNAGC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 9, 618
BsaXI ACNNNNNCTCC 4 cut(s) 363, 393, 513, 543
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 3 cut(s) 11, 316, 620
BseDI CCNNGG 2 cut(s) 9, 618
BseGI GGATG 1 cut(s) 631
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 4 cut(s) 212, 240, 314, 671
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 3 cut(s) 190, 451, 630
BseYI CCCAGC 1 cut(s) 472
BshFI GGCC 1 cut(s) 328
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 58
BsnI GGCC 1 cut(s) 328
Bsp1286I GDGCHC 2 cut(s) 8, 58
Bsp1407I TGTACA 1 cut(s) 275
Bsp143I GATC 1 cut(s) 138
BspANI GGCC 1 cut(s) 328
BspCNI CTCAG 4 cut(s) 211, 239, 315, 670
BspLI GGNNCC 2 cut(s) 7, 265
BspMAI CTGCAG 1 cut(s) 648
BspPI GGATC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 5
BsrDI GCAATG 1 cut(s) 459
BsrGI TGTACA 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 9, 618
BssMI GATC 1 cut(s) 138
Bst2UI CCWGG 3 cut(s) 11, 316, 620
Bst6I CTCTTC 2 cut(s) 100, 118
BstAUI TGTACA 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 4 cut(s) 198, 226, 323, 657
BstF5I GGATG 1 cut(s) 631
BstHHI GCGC 2 cut(s) 565, 683
BstKTI GATC 1 cut(s) 141
BstMBI GATC 1 cut(s) 138
BstMWI GCNNNNNNNGC 3 cut(s) 74, 203, 464
BstNI CCWGG 3 cut(s) 11, 316, 620
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 3 cut(s) 9, 314, 618
BstSFI CTRYAG 1 cut(s) 644
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 190, 451, 630
BstXI CCANNNNNNTGG 1 cut(s) 315
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 1 cut(s) 328
BtsCI GGATG 1 cut(s) 631
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 3 cut(s) 278, 320, 531
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 2 cut(s) 565, 683
Cfr13I GGNCC 1 cut(s) 419
Csp6I GTAC 2 cut(s) 276, 686
CviAII CATG 4 cut(s) 174, 399, 503, 689
CviQI GTAC 2 cut(s) 276, 686
DdeI CTNAG 4 cut(s) 198, 226, 323, 657
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 241
DseDI GACNNNNNNGTC 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 100, 118
EarI CTCTTC 2 cut(s) 100, 118
Eco147I AGGCCT 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 3 cut(s) 9, 314, 618
FaeI CATG 4 cut(s) 177, 402, 506, 692
FatI CATG 4 cut(s) 173, 398, 502, 688
FblI GTMKAC 1 cut(s) 119
Fnu4HI GCNGC 3 cut(s) 204, 465, 644
FokI GGATG 1 cut(s) 638
Fsp4HI GCNGC 3 cut(s) 204, 465, 644
FspBI CTAG 1 cut(s) 488
GlaI GCGC 2 cut(s) 564, 682
GluI GCNGC 3 cut(s) 204, 465, 644
GsaI CCCAGC 1 cut(s) 476
HaeIII GGCC 1 cut(s) 328
HhaI GCGC 2 cut(s) 565, 683
Hin1II CATG 4 cut(s) 177, 402, 506, 692
Hin6I GCGC 2 cut(s) 563, 681
HinP1I GCGC 2 cut(s) 563, 681
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HinfI GANTC 3 cut(s) 92, 304, 614
HphI GGTGA 1 cut(s) 623
Hpy166II GTNNAC 2 cut(s) 120, 276
Hpy188I TCNGA 2 cut(s) 229, 660
Hpy188III TCNNGA 2 cut(s) 24, 89
Hpy8I GTNNAC 2 cut(s) 120, 276
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 1 cut(s) 256
HpyCH4V TGCA 6 cut(s) 161, 182, 206, 394, 405, 646
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 203, 464
HpyF3I CTNAG 4 cut(s) 198, 226, 323, 657
Hsp92II CATG 4 cut(s) 177, 402, 506, 692
HspAI GCGC 2 cut(s) 563, 681
Kzo9I GATC 1 cut(s) 138
LmnI GCTCC 3 cut(s) 26, 61, 521
Lsp1109I GCAGC 3 cut(s) 190, 451, 630
MaeI CTAG 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 37, 379
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MboII GAAGA 7 cut(s) 87, 105, 162, 343, 506, 617, 673
MhlI GDGCHC 2 cut(s) 8, 58
MluCI AATT 4 cut(s) 84, 133, 207, 531
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 487
MseI TTAA 2 cut(s) 213, 694
MspR9I CCNGG 3 cut(s) 11, 316, 620
MvaI CCWGG 3 cut(s) 11, 316, 620
MwoI GCNNNNNNNGC 3 cut(s) 74, 203, 464
NdeII GATC 1 cut(s) 138
NlaIII CATG 4 cut(s) 177, 402, 506, 692
NlaIV GGNNCC 2 cut(s) 7, 265
NmuCI GTSAC 1 cut(s) 37
NspI RCATGY 1 cut(s) 692
PceI AGGCCT 1 cut(s) 328
PciI ACATGT 1 cut(s) 688
PfeI GAWTC 2 cut(s) 304, 614
PkrI GCNGC 3 cut(s) 205, 466, 645
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 688
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 3 cut(s) 9, 314, 618
PspFI CCCAGC 1 cut(s) 472
PspGI CCWGG 3 cut(s) 9, 314, 618
PspN4I GGNNCC 2 cut(s) 7, 265
PspPI GGNCC 1 cut(s) 419
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 1 cut(s) 648
RsaI GTAC 2 cut(s) 277, 687
RsaNI GTAC 2 cut(s) 276, 686
SalI GTCGAC 1 cut(s) 118
SaqAI TTAA 2 cut(s) 213, 694
SatI GCNGC 3 cut(s) 204, 465, 644
Sau3AI GATC 1 cut(s) 138
Sau96I GGNCC 1 cut(s) 419
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 3 cut(s) 11, 316, 620
SduI GDGCHC 2 cut(s) 8, 58
SetI ASST 9 cut(s) 79, 113, 151, 169, 342, 364, 376, 424, 586
SfcI CTRYAG 1 cut(s) 644
SinI GGWCC 1 cut(s) 419
Sse9I AATT 4 cut(s) 84, 133, 207, 531
SseBI AGGCCT 1 cut(s) 328
SspMI CTAG 1 cut(s) 488
StuI AGGCCT 1 cut(s) 328
StyD4I CCNGG 3 cut(s) 9, 314, 618
TaqI TCGA 1 cut(s) 119
TasI AATT 4 cut(s) 84, 133, 207, 531
TatI WGTACW 2 cut(s) 275, 685
TfiI GAWTC 2 cut(s) 304, 614
Tru1I TTAA 2 cut(s) 213, 694
Tru9I TTAA 2 cut(s) 213, 694
TscAI CASTG 3 cut(s) 278, 327, 531
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 203, 464, 643
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 143, 162
TspGWI ACGGA 1 cut(s) 564
TspRI CASTG 3 cut(s) 278, 327, 531
VpaK11BI GGWCC 1 cut(s) 419
XceI RCATGY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 119
XspI CTAG 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.