RLG00000004453

ATP synthase subunit a, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017493
Physical Location & Seq
Forward (+)
59476670 .. 59477364
695 bp
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UTR
Exon/CDS
Intron
RLM00000004453

Sequence Viewer

Length: 540 bp
ATGCCGGTGGTCCTTGTGAGGAATATGGTTGAGATGAATGTCATCGGCGCACTTCGTCACAGAGGATGGAACAATCAAGTTGTAATCAATGCTGCCACTGAAGGTTTAGAAAATGCAATGAATCAGAGAGCTGAAGGATTCGGTATTCGGATTGCTTTTCATGAGCCTATTCCGGATGTTATTCCTCCTCCACCTGGACTTCCTCATCCGTTTGTTCCCATATTTGCTCCTCCGGGCGGTGCTGTTGGGCAGCGTCCTCCTCTGATGATTCCTGGAGTTGCACCTTGTGTGCTTGCTTGTGGTCTTGGAGATATGTTCTTGATCTTAGAAGATTTCACAAAACTGTTGTCGCTTAGTTTTCGACTTTTCAGAAATATTCTAGCTGATGAATTAGTAGTTGTTGTTCTTGTTTCTTTAGTACCTTTAGTAGTTCCTATACTTGTCATGTTCCTTGGATTATTAACAAGCAGTATTCAAGCTCTTATTTTTGCAACCCCAGCTGCGACTTATATAGGCGAATCCATGGAAGGTCATCATTGA

Protein Analysis

180

Amino Acids

19.27

Weight (kDa)

5.65

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_A PF00119 106 - 171 2e-11 ATP synthase A chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017493)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00150
malus_domestica MD02G1261800.v1.1 MD16G1100100.v1.1 MD16G1263300.v1.1
pyrus_communis pycom08g10380
rosa_chinensis RchiOBHm_Chr2g0129011 RchiOBHm_CPg0502431
rosa_laevigata RLG00000004365 RLG00000004453
rosa_rugosa Rorug02G0490900
rosa_samantha Rh2BG343800 Rh6AG047100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 172
AciI CCGC 1 cut(s) 237
AcuI CTGAAG 2 cut(s) 120, 153
AdeI CACNNNGTG 1 cut(s) 287
AfaI GTAC 1 cut(s) 420
AfiI CCNNNNNNNGG 2 cut(s) 194, 236
AgsI TTSAA 1 cut(s) 476
AjnI CCWGG 2 cut(s) 193, 271
AluBI AGCT 4 cut(s) 131, 383, 479, 500
AluI AGCT 4 cut(s) 131, 383, 479, 500
Aor13HI TCCGGA 1 cut(s) 172
ApeKI GCWGC 3 cut(s) 92, 250, 500
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 10
AsuC2I CCSGG 1 cut(s) 234
AvaII GGWCC 1 cut(s) 10
BbvI GCAGC 3 cut(s) 79, 262, 487
BccI CCATC 1 cut(s) 60
BciT130I CCWGG 2 cut(s) 195, 273
BcnI CCSGG 1 cut(s) 234
BfaI CTAG 1 cut(s) 380
BisI GCNGC 3 cut(s) 93, 251, 501
BlsI GCNGC 3 cut(s) 94, 252, 502
Bme1390I CCNGG 3 cut(s) 195, 234, 273
Bme18I GGWCC 1 cut(s) 10
BmgT120I GGNCC 1 cut(s) 10
BmrFI CCNGG 3 cut(s) 195, 234, 273
BpmI CTGGAG 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 451, 522
BsaWI WCCGGW 1 cut(s) 172
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 236
Bse118I RCCGGY 1 cut(s) 4
Bse3DI GCAATG 1 cut(s) 123
BseAI TCCGGA 1 cut(s) 172
BseBI CCWGG 2 cut(s) 195, 273
BseDI CCNNGG 2 cut(s) 451, 522
BseGI GGATG 3 cut(s) 71, 181, 205
BseLI CCNNNNNNNGG 2 cut(s) 194, 236
BseMI GCAATG 1 cut(s) 123
BseRI GAGGAG 3 cut(s) 177, 219, 249
BseXI GCAGC 3 cut(s) 79, 262, 487
BseYI CCCAGC 1 cut(s) 496
BsiSI CCGG 3 cut(s) 5, 173, 233
BslI CCNNNNNNNGG 2 cut(s) 194, 236
Bsp13I TCCGGA 1 cut(s) 172
Bsp143I GATC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 1 cut(s) 237
BspEI TCCGGA 1 cut(s) 172
BspHI TCATGA 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 451, 522
BssMI GATC 1 cut(s) 321
BssT1I CCWWGG 2 cut(s) 451, 522
Bst2UI CCWGG 2 cut(s) 195, 273
Bst4CI ACNGT 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 325, 353
BstDSI CCRYGG 1 cut(s) 522
BstF5I GGATG 3 cut(s) 71, 181, 205
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 1 cut(s) 324
BstMBI GATC 1 cut(s) 321
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 2 cut(s) 195, 273
BstSCI CCNGG 3 cut(s) 193, 232, 271
BstV1I GCAGC 3 cut(s) 79, 262, 487
BtgI CCRYGG 1 cut(s) 522
BtsCI GGATG 3 cut(s) 71, 181, 205
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 294
CciI TCATGA 1 cut(s) 160
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 10
CseI GACGC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 419
CviAII CATG 3 cut(s) 161, 445, 523
CviJI RGCY 5 cut(s) 131, 166, 383, 479, 500
CviKI_1 RGCY 5 cut(s) 131, 166, 383, 479, 500
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 2 cut(s) 325, 353
DpnI GATC 1 cut(s) 323
DpnII GATC 1 cut(s) 321
DraIII CACNNNGTG 1 cut(s) 287
Eco130I CCWWGG 2 cut(s) 451, 522
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 120, 153
EcoRII CCWGG 2 cut(s) 193, 271
EcoT14I CCWWGG 2 cut(s) 451, 522
ErhI CCWWGG 2 cut(s) 451, 522
FaeI CATG 3 cut(s) 164, 448, 526
FaiI YATR 9 cut(s) 26, 162, 221, 314, 437, 446, 510, 512, 524
FatI CATG 3 cut(s) 160, 444, 522
Fnu4HI GCNGC 3 cut(s) 93, 251, 501
FokI GGATG 3 cut(s) 78, 188, 192
Fsp4HI GCNGC 3 cut(s) 93, 251, 501
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 49
GluI GCNGC 3 cut(s) 93, 251, 501
GsaI CCCAGC 1 cut(s) 500
GsuI CTGGAG 1 cut(s) 294
HapII CCGG 3 cut(s) 5, 173, 233
HgaI GACGC 1 cut(s) 242
HhaI GCGC 1 cut(s) 50
Hin1II CATG 3 cut(s) 164, 448, 526
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HinfI GANTC 4 cut(s) 121, 138, 268, 518
HpaII CCGG 3 cut(s) 5, 173, 233
Hpy188I TCNGA 4 cut(s) 126, 150, 264, 371
Hpy188III TCNNGA 3 cut(s) 161, 173, 319
HpyAV CCTTC 3 cut(s) 95, 128, 521
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 116, 281, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 2 cut(s) 325, 353
Hsp92II CATG 3 cut(s) 164, 448, 526
HspAI GCGC 1 cut(s) 48
Kpn2I TCCGGA 1 cut(s) 172
Kzo9I GATC 1 cut(s) 321
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 8 cut(s) 18, 180, 186, 207, 246, 258, 285, 510
Lsp1109I GCAGC 3 cut(s) 79, 262, 487
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 1 cut(s) 56
MalI GATC 1 cut(s) 323
MboI GATC 1 cut(s) 321
MboII GAAGA 1 cut(s) 341
MluCI AATT 1 cut(s) 389
MnlI CCTC 8 cut(s) 12, 56, 195, 198, 213, 240, 267, 270
MroI TCCGGA 1 cut(s) 172
MseI TTAA 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 3 cut(s) 5, 173, 233
MspR9I CCNGG 3 cut(s) 195, 234, 273
MvaI CCWGG 2 cut(s) 195, 273
MwoI GCNNNNNNNGC 1 cut(s) 497
NciI CCSGG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 522
NdeII GATC 1 cut(s) 321
NlaIII CATG 3 cut(s) 164, 448, 526
NmuCI GTSAC 1 cut(s) 56
PagI TCATGA 1 cut(s) 160
PfeI GAWTC 4 cut(s) 121, 138, 268, 518
PfoI TCCNGGA 1 cut(s) 271
PkrI GCNGC 3 cut(s) 94, 252, 502
Psp6I CCWGG 2 cut(s) 193, 271
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 2 cut(s) 193, 271
PspPI GGNCC 1 cut(s) 10
PsrI GAACNNNNNNTAC 2 cut(s) 387, 419
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 3 cut(s) 93, 251, 501
Sau3AI GATC 1 cut(s) 321
Sau96I GGNCC 1 cut(s) 10
ScrFI CCNGG 3 cut(s) 195, 234, 273
SetI ASST 9 cut(s) 106, 133, 196, 286, 385, 424, 481, 502, 532
SinI GGWCC 1 cut(s) 10
Sse9I AATT 1 cut(s) 389
SsiI CCGC 1 cut(s) 237
SspI AATATT 1 cut(s) 376
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 193, 232, 271
StyI CCWWGG 2 cut(s) 451, 522
TaaI ACNGT 1 cut(s) 345
TaqI TCGA 1 cut(s) 361
TasI AATT 1 cut(s) 389
TfiI GAWTC 4 cut(s) 121, 138, 268, 518
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 3 cut(s) 92, 250, 500
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 4 cut(s) 50, 134, 149, 402
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 10
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.