Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002301

Physical Location & Seq

Genomic Coordinates Forward (+)

59903476 .. 59911107

Gene Structure 7632 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004502

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4302 bp

ATGGCGACAGGGCAGGTTGATGTTTTGAGAAGAAGGGATTCTCCTAAATACTCCAGGAGAGAATTTGGGTATTGTAAGAATGGGTTCTCTGGTGTTAAGCTGAGCCATGGCCGTAATGATGGTGGCAAATTTAGCGATGAAGTTAGCGATTCGCGAAGATGGAGAGGGCAATCAAATGCCTCAGAATCGTTGGTTGAGACCAGAAAAACTCCGGAGAAGGAGAAGGAGAAGAAGAGGAAGTTTTCTCCTGTTATATGGGACAGAGAAGAGAAGAAATTAGTAAGGGTATCATTGAAGAATGGAGTTGTTCCAGTTTCAGTTGTTTCTCGGTCATCTCCTCTAAAGAGATCATCAAGATCACCATCTTGTGAGGTTCATGATGTTGTCTCCGAAGGAGTCGACAGAGTTCAATTGCTTCCTGAGATCAGGAAGAGGAAGTCTCCGGTTACAAGTGACAGGGAAGAGAAGAGAGTGAGGATATCATCAAAGAATAGATTTGTCCCGGTCACTCCTCCATCTAGTGTAATTGTTTCCGATGGGGTTGTTTCAAATTCTCCTGCTTCACAGACTCAGTCTCATGAAGTGGAGGGTTCTGAAGATAAACCTTCTGTGGCAGATGGGTTGGTGGAGATTTCTCAAATGGAAGACCCTGCTGATTTGCAGGTTCTGCGGCATTCCGAGGTTCCAAATAACCTGGATGACAAAGACCAGACTAGAATCTCATTGTCAAGATGGGCATCTGACATTGACTCTCCAAGGGATGATGTTAGCTGCAGCTCGACTCCTGAAAATGGCGAAATCCGAAGAGAAGGCTCAGTTGGGGATAGGAGCAGAACTTCTAGCTTTGTCAGTGGAGATGGTGGAGAAGAGATTAGTGGACATGTTGGCAATGCGGGCGTTGATCAGCTGTATCCTGATTCAGATGACGAAAATATTGCTCGGATTGAAGAGCCTGCAGTGCCCAGGGAAAGAAGTACAAACATGCTTCAGGGTTGCAGAAGTAGTCAGGAGTACTATACTAGTGATCAAGAGGCACTGGGTGAAGGCACTTATGGTGTAGTCTACAGAGCGAGAGATGAACAAACCGGGGAAATTGTAGCCATGAAGAAAGTGAAGATGGATAAGTGCGATGAAGGTTTCCCTATTACATCATTGAGAGAAATAAACATTCTTTTGTCATGTAATCACCCTTCCATTGTGGGCGTGAAGGAAGTTGTTGTGGACAAGGATGATGATATTCTTATGGTTATGGAGTATATGGACTATGAGCTCAAGTTTCTAATGGAATCGATGGAGCAGCGATTTAGTATAGGCGAAATTAAATACTTGGTGAAAAGGCTGTTGGAGGGTGTAAAGTATCTTCATGACAATTGGGTCCTTCACAGAGATTTGAAAACTTCAAACATGCTTTTGAACAAGGAGGGTGAATTGAAAATATGTGACCTTGGGTTGTCACGCCAGTATGGGAGTCCCCTGAAGCCTTATACTTCCAATGTAGTCACTTTGTTGTACAGGGCACCTGAACTTCTATTAGGTCAAAAGCGGTACTCTACAGCAATTGATATGTGGTCAGTTGGATGTATAATGGCTGAACTTTTGGCCAAAGAACCACTATTCAGTCCGAAAACGCCAACCGAAGTTCATCAGCTTGACGCGATTTTTGATACTCTTGGTGCACCAAACGAGACAGATTGGCCTGGAGTATCCAAATTGCCAGTGTTTAAAGCTATTAAGATTAAAAAAGGGGAGAACAATCTGCGCAAGAAATTTTGTGGGACATTTTTCACAGGATCACCAATGCTTGCTGCTTCAGGGTTGGATCTGTTGACAAAACTTTTAACATATGATCCTGAGAAGCGGATAACAGTGGATGATGCTCTTAGCCATGACTGGTTTAAGGACTACCCTGAACCCAGACCTTTTAAACCTTCTCCTAGTGTTCATGACAGCTACATCCTCAAGCAGCAAGGGGAAAGGAAGCGCGTTTGTGAAGGTAACAGAGTGCTGGGTCTTCTAGAAGGTACAGAAAATGAGTTGCAAGGTTATGTAGCTCTGTATATAAAGCTAGACCACTTGGCTGGTGAGGTTTCATTTATAAGTTCAAGATTCCAAGGGCTTCCGCTTTTATCCAAATTTGTTGTTAGGCGTTCGTATCGATCTCTACCGCTTCTCACCGGCGATCTCGATTTCGGGAGAGTTGACGTTTTTAGAAGAAGGGATTCTCCTAAGTACTCCAAAAGAGAATCTGAGTATTATAGGAACGGGTTCTCTGGTGTTAAGCTGAGCCATGGCGGTGATGGGGGTGTCAAATATAGGGATGAAGTTGGCAATTCAAGGAGGTCGGTTGAGAATGGAAGGGGTCCTCATCGGGAAGGGGTGGTTGAGTTACTTCCTGAGAAGAAGAGGAAGTTTTCGCCCATTATATGGGATAAAGAAGAGAAGAAAGCGAGGGCATTAACAAGGAGTGGGGTTGTTCCTGTCTCGGCCATTGCTCATTCATCACCTCGGAAGAGATCATCTTTTGAGGTTGGTGATGTTGTCTCTGATGGTCCTGCTTCAGATATTCAGTATCATGGAGAGGGGGTTTCTGAATTCAAGCATGAAAATGTGGTTCCGTTACTCTCTGAGAAGAAGAGGAAGTCTCCTGTTACAATTGACAGAGAAGAGAAGAAAGTGAGGATATCATCAAAGAACAGAGTTGTTAAAGTTGTTCCTGTCATTTCTTCTACATCTCCCCGTCCTGCTCAAACCAATGGGGTTGCTTCAAAGTCTCCTGCTTCAGAGATTCAGTCTCTGGGAGTGGAGGTGTCTGAAGCTGAACCTTTTGTGGCTGATGGGCTGGTGGAGATTTCTCATTTGGAACCTCCCGCTGATGTGCATACTCCACTATGCAAATCCGAGGCACAGAGTAACCTGAATGACAAAGACCAGACTAGGATCTCATTGTCAAGATGGGCATCTGACACTGATTCTCCGATGGATGATCGCAGCTGTAACTCAACTCCAGAAAGTGGCGAAATCCGAAGAGAAGACTCAGTTGAGCCTCGGAGCAGAACTTCTAGCTCTCATGAAGAAAGCGACTCTGTCGCTAGAGAAGAGATTGGTGGGGTTGGTGGGAATGCAAGTGGTGGCCAGCTGTATCCTGACTCGGAGGATGATGAAGGTCTTCCTCAGATTGAAGAGCCCGTGGTGCCAAGAGGAAGAAGTACAAACATGCTTCAGGGATGCAGAAGTGTGGCCGAGTTTACTAAACTAGATGGGATGCTGGGTGAAGGCACTTATGGTGTTGTCTACAGAGCGAGAGATAAACAAACCGGAGAAATTGTAGCCATTAAGAAGGTGAAGATGGATAAGTCCAATGAAGGTTTCCCTATTACATCATTGAGAGAAATAAACATTCTTTTGTCTTGTAATCACCCTTCCATTGTGGGTGTGAAGGAAGTTGTTCACAACGGTGATGATATTTTTATGGTTATGGAGTGTATGGAATATGAGCTCAAGCTTCTAACGGAGGCGACAAAACAGCCATTTAGTATAGGCGAAGTTAAATACTTGATGAGAAGTCTGTTGGAGGGTGTGAAGTATCTTCATGGCAATTGGGTTCTTCACAGAGATCTGAAAACTTCAAATATTCTTTTAAACAAGGAGGGTGAATTGAAAATATGCGACTTTGGTTTGTCACGCCAGTATGGGAGTCCCCTGAAGCCGTATACTTCCCTAGTGGTCACTTTGTGGTACAGGGCACCTGAACTTCTATTAGGTCAAAAGCAGTATTCTACGGCAATTGATATGTGGTCAGTTGGATGTATAATGGCTGAACTTTTGGCCAAGGAAGCTCTATTTCAAGGGAAAACCGAAGTTGATCAGCTTGACAAGATTTTCAGAACTCTTGGTACACCAAGTGAGACAGATTGGCCTGGGGTATCCAAATTGCCTGGCTTCAAAGCTATTCAGGTTAAACAACGGGAGAACCTTTTGCGCAAGAAATTTTCTGGGACATGTTTCACAGGATCCCCAATGCTCTCTGCTTCAGGGTTGGATTTGTTGACAAAACTTTTAACATACGACCCTGCGAAGCGGATAACAGTGGATGATGCTCTTAGCCATGACTGGTTTAAAGACTATCCCGAACCCAGACCTTTCAAGCCTTCGCCTACTGTGCATGACAGTCTCAAAAGAAGGGGAGGATGCGATACTGCATTTCAGACATGGTTTTGCAAATGTACTCAAATTCTATGCATACTTCAGGGTGTCTGGCTTGGTTGTAGTTCCCCTTTCGGTTTGATTGCTACTTCATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006109 GO:0006139 GO:0006396 GO:0006397 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008380 GO:0009555 GO:0009653 GO:0009889 GO:0009987 GO:0010208 GO:0010467 GO:0010468 GO:0010556 GO:0010584 GO:0010675 GO:0010927 GO:0010962 GO:0016043 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0022607 GO:0030198 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032881 GO:0032885 GO:0032950 GO:0032951 GO:0032952 GO:0032953 GO:0032989 GO:0034641 GO:0036211 GO:0043062 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045229 GO:0046483 GO:0048229 GO:0048646 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0085029 GO:0090304 GO:0097472 GO:0140096 GO:1901360 GO:1901564 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1434

Amino Acids

160.06

Weight (kDa)

6.66

Isoelectric Point (pI)

53.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	343 - 632	2.5e-69	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	343 - 537	2.2e-31	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	1085 - 1277	2.9e-32	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	1087 - 1372	6.8e-74	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63370 AT5G63370 AT5G63370 AT5G63370 AT5G63370 AT5G63370 AT5G63370 AT5G63370 AT5G63370 AT5G63370
fragaria_vesca	FvH4_5g07740
malus_domestica	MD06G1147400.v1.1 MD06G1147600.v1.1 MD14G1161600.v1.1
prunus_persica	Prupe.5G154500_v2.0.a1 Prupe.5G154500_v2.0.a1 Prupe.5G154500_v2.0.a1 Prupe.5G154500_v2.0.a1 Prupe.5G154500_v2.0.a1 Prupe.5G154500_v2.0.a1 Prupe.5G154500_v2.0.a1
pyrus_communis	pycom06g13700
rosa_chinensis	RchiOBHm_Chr7g0190701 RchiOBHm_Chr7g0190711
rosa_laevigata	RLG00000004502
rosa_multiflora	Rmu_co8410623.1_g000001 Rmu_sc0006709.1_g000001 Rmu_sc0014053.1_g000001 Rmu_sc0021017.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264390
rosa_rugosa	Rorug06G0506600 Rorug06G0506700
rosa_samantha	Rh7AG113100 Rh7AG113200 Rh7BG115600 Rh7BG115700 Rh7CG118100 Rh7CG118200 Rh7DG117000 Rh7DG117100
rosa_wichuraiana	Rw7G009870 Rw7G009880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2096
AasI	GACNNNNNNGTC	1 cut(s)	1373
Acc16I	TGCGCA	2 cut(s)	1760, 3980
Acc36I	ACCTGC	2 cut(s)	4, 652
AccB1I	GGYRCC	3 cut(s)	1516, 3190, 3742
AccB7I	CCANNNNNTGG	2 cut(s)	2424, 3011
AccI	GTMKAC	4 cut(s)	399, 1062, 3291, 3710
AccII	CGCG	3 cut(s)	154, 1655, 1983
AccIII	TCCGGA	1 cut(s)	211
AciI	CCGC	9 cut(s)	670, 893, 1543, 1858, 2120, 2165, 2292, 2868, 4078
AclWI	GGATC	6 cut(s)	1798, 1827, 1841, 2945, 4005, 4018
AcoI	YGGCCR	6 cut(s)	109, 1599, 2484, 3130, 3237, 3825
AcsI	RAATTY	8 cut(s)	62, 128, 550, 1766, 2132, 2591, 3986, 4230
AdeI	CACNNNGTG	3 cut(s)	1040, 3732, 3902
AfiI	CCNNNNNNNGG	3 cut(s)	791, 2424, 3011
AflIII	ACRYGT	2 cut(s)	880, 3998
AhlI	ACTAGT	1 cut(s)	1019
AjnI	CCWGG	6 cut(s)	53, 693, 962, 1696, 3916, 3934
AjuI	GAANNNNNNNTTGG	6 cut(s)	801, 833, 1325, 1357, 3350, 3382
AleI	CACNNNNGTG	1 cut(s)	3453
Alw21I	GWGCWC	3 cut(s)	1272, 1678, 3498
Alw44I	GTGCAC	1 cut(s)	1674
AlwI	GGATC	6 cut(s)	1798, 1827, 1841, 2945, 4005, 4018
AlwNI	CAGNNNCTG	2 cut(s)	667, 2794
Aor13HI	TCCGGA	1 cut(s)	211
AoxI	GGCC	8 cut(s)	109, 1599, 1694, 2484, 3130, 3237, 3825, 3914
ApaLI	GTGCAC	1 cut(s)	1674
ApeKI	GCWGC	6 cut(s)	771, 774, 1297, 1805, 1963, 2988
ApoI	RAATTY	8 cut(s)	62, 128, 550, 1766, 2132, 2591, 3986, 4230
ArsI	GACNNNNNNTTYG	2 cut(s)	1643, 1675
Asp700I	GAANNNNTTC	6 cut(s)	37, 83, 2218, 2264, 3165, 3444
AspLEI	GCGC	3 cut(s)	1761, 1983, 3981
AspS9I	GGNCC	3 cut(s)	1375, 2360, 2549
AsuC2I	CCSGG	2 cut(s)	503, 1087
AvaII	GGWCC	3 cut(s)	1375, 2360, 2549
BaeGI	GKGCMC	4 cut(s)	963, 1519, 1678, 3745
BalI	TGGCCA	3 cut(s)	1601, 3132, 3827
BamHI	GGATCC	1 cut(s)	4010
BanI	GGYRCC	3 cut(s)	1516, 3190, 3742
BanII	GRGCYC	3 cut(s)	1272, 3186, 3498
BarI	GAAGNNNNNNTAC	6 cut(s)	1174, 1206, 1344, 1376, 3403, 3435
BbsI	GAAGAC	4 cut(s)	651, 2003, 3036, 3158
Bbv12I	GWGCWC	3 cut(s)	1272, 1678, 3498
BbvI	GCAGC	6 cut(s)	758, 786, 1309, 1792, 1975, 3000
BceAI	ACGGC	3 cut(s)	96, 3691, 3795
BcgI	CGANNNNNNTGC	2 cut(s)	917, 951
BciT130I	CCWGG	6 cut(s)	55, 695, 964, 1698, 3918, 3936
BciVI	GTATCC	4 cut(s)	921, 1714, 3150, 3934
BclI	TGATCA	3 cut(s)	901, 1024, 3862
BcnI	CCSGG	2 cut(s)	503, 1087
BcuI	ACTAGT	1 cut(s)	1019
BfmI	CTRYAG	5 cut(s)	772, 954, 1063, 1551, 3292
BfuAI	ACCTGC	2 cut(s)	4, 652
BfuI	GTATCC	4 cut(s)	921, 1714, 3150, 3934
BglII	AGATCT	1 cut(s)	3613
BisI	GCNGC	7 cut(s)	671, 772, 775, 1298, 1806, 1964, 2989
BlpI	GCTNAGC	2 cut(s)	101, 2282
BlsI	GCNGC	7 cut(s)	672, 773, 776, 1299, 1807, 1965, 2990
BmcAI	AGTACT	2 cut(s)	1013, 2231
Bme1390I	CCNGG	8 cut(s)	55, 503, 695, 964, 1087, 1698, 3918, 3936
Bme18I	GGWCC	3 cut(s)	1375, 2360, 2549
BmgT120I	GGNCC	3 cut(s)	1375, 2360, 2549
BmiI	GGNNCC	9 cut(s)	684, 1376, 1518, 2361, 2613, 2862, 3192, 3744, 4012
BmrFI	CCNGG	8 cut(s)	55, 503, 695, 964, 1087, 1698, 3918, 3936
BmrI	ACTGGG	1 cut(s)	1046
BmsI	GCATC	7 cut(s)	746, 1864, 2966, 3215, 3252, 4084, 4178
BmuI	ACTGGG	1 cut(s)	1046
BpiI	GAAGAC	4 cut(s)	651, 2003, 3036, 3158
BplI	GAGNNNNNCTC	4 cut(s)	424, 456, 2626, 2658
BpmI	CTGGAG	3 cut(s)	37, 1719, 2988
Bpu1102I	GCTNAGC	2 cut(s)	101, 2282
BpuEI	CTTGAG	3 cut(s)	1256, 1943, 3482
BpuMI	CCSGG	2 cut(s)	503, 1087
Bsa29I	ATCGAT	2 cut(s)	1289, 2155
BsaBI	GATNNNNATC	3 cut(s)	361, 736, 2956
BsaI	GGTCTC	1 cut(s)	191
BsaWI	WCCGGW	3 cut(s)	211, 442, 3314
BsaXI	ACNNNNNCTCC	8 cut(s)	2568, 2598, 2790, 2820, 2956, 2986, 3309, 3339
Bsc4I	CCNNNNNNNGG	3 cut(s)	791, 2424, 3011
Bse118I	RCCGGY	1 cut(s)	2174
Bse1I	ACTGG	7 cut(s)	311, 1041, 1459, 1715, 1895, 3685, 4115
Bse3DI	GCAATG	2 cut(s)	895, 2487
Bse8I	GATNNNNATC	3 cut(s)	361, 736, 2956
BseAI	TCCGGA	1 cut(s)	211
BseBI	CCWGG	6 cut(s)	55, 695, 964, 1698, 3918, 3936
BseCI	ATCGAT	2 cut(s)	1289, 2155
BseJI	GATNNNNATC	3 cut(s)	361, 736, 2956
BseLI	CCNNNNNNNGG	3 cut(s)	791, 2424, 3011
BseMI	GCAATG	2 cut(s)	895, 2487
BseNI	ACTGG	7 cut(s)	311, 1041, 1459, 1715, 1895, 3685, 4115
BseRI	GAGGAG	2 cut(s)	327, 501
BseSI	GKGCMC	4 cut(s)	963, 1519, 1678, 3745
BseXI	GCAGC	6 cut(s)	758, 786, 1309, 1792, 1975, 3000
BseYI	CCCAGC	2 cut(s)	2005, 3265
Bsh1236I	CGCG	3 cut(s)	154, 1655, 1983
BshFI	GGCC	8 cut(s)	111, 1601, 1696, 2486, 3132, 3239, 3827, 3916
BshNI	GGYRCC	3 cut(s)	1516, 3190, 3742
BshVI	ATCGAT	2 cut(s)	1289, 2155
BsiHKAI	GWGCWC	3 cut(s)	1272, 1678, 3498
BsiSI	CCGG	6 cut(s)	212, 443, 503, 1086, 2175, 3315
BslFI	GGGAC	6 cut(s)	272, 485, 1455, 1789, 3681, 4009
BslI	CCNNNNNNNGG	3 cut(s)	791, 2424, 3011
BsmFI	GGGAC	6 cut(s)	272, 485, 1455, 1789, 3681, 4009
BsmI	GAATGC	2 cut(s)	673, 3124
BsnI	GGCC	8 cut(s)	111, 1601, 1696, 2486, 3132, 3239, 3827, 3916
Bso31I	GGTCTC	1 cut(s)	191
Bsp1286I	GDGCHC	7 cut(s)	963, 1272, 1519, 1678, 3186, 3498, 3745
Bsp13I	TCCGGA	1 cut(s)	211
Bsp1407I	TGTACA	1 cut(s)	1509
Bsp1720I	GCTNAGC	2 cut(s)	101, 2282
Bsp19I	CCATGG	2 cut(s)	106, 2287
Bsp68I	TCGCGA	1 cut(s)	154
BspACI	CCGC	9 cut(s)	670, 893, 1543, 1858, 2120, 2165, 2292, 2868, 4078
BspANI	GGCC	8 cut(s)	111, 1601, 1696, 2486, 3132, 3239, 3827, 3916
BspDI	ATCGAT	2 cut(s)	1289, 2155
BspEI	TCCGGA	1 cut(s)	211
BspFNI	CGCG	3 cut(s)	154, 1655, 1983
BspHI	TCATGA	5 cut(s)	376, 577, 1363, 1942, 3067
BspLI	GGNNCC	9 cut(s)	684, 1376, 1518, 2361, 2613, 2862, 3192, 3744, 4012
BspMAI	CTGCAG	2 cut(s)	776, 958
BspMI	ACCTGC	2 cut(s)	4, 652
BspPI	GGATC	6 cut(s)	1798, 1827, 1841, 2945, 4005, 4018
BspQI	GCTCTTC	2 cut(s)	942, 3174
BspT107I	GGYRCC	3 cut(s)	1516, 3190, 3742
BspTNI	GGTCTC	1 cut(s)	191
BsrDI	GCAATG	2 cut(s)	895, 2487
BsrFI	RCCGGY	1 cut(s)	2174
BsrGI	TGTACA	1 cut(s)	1509
BsrI	ACTGG	7 cut(s)	311, 1041, 1459, 1715, 1895, 3685, 4115
BssAI	RCCGGY	1 cut(s)	2174
BssNAI	GTATAC	1 cut(s)	3711
BssT1I	CCWWGG	6 cut(s)	106, 755, 1444, 2110, 2287, 3828
Bst1107I	GTATAC	1 cut(s)	3711
Bst2UI	CCWGG	6 cut(s)	55, 695, 964, 1698, 3918, 3936
Bst4CI	ACNGT	5 cut(s)	1867, 3455, 4087, 4159, 4169
BstAPI	GCANNNNNTGC	1 cut(s)	667
BstAUI	TGTACA	1 cut(s)	1509
BstC8I	GCNNGC	4 cut(s)	895, 954, 1803, 3134
BstDSI	CCRYGG	3 cut(s)	106, 2287, 3186
BstFNI	CGCG	3 cut(s)	154, 1655, 1983
BstHHI	GCGC	3 cut(s)	1761, 1983, 3981
BstMWI	GCNNNNNNNGC	7 cut(s)	132, 667, 894, 958, 3190, 3833, 4159
BstNI	CCWGG	6 cut(s)	55, 695, 964, 1698, 3918, 3936
BstNSI	RCATGY	5 cut(s)	884, 985, 1408, 3217, 4002
BstSCI	CCNGG	8 cut(s)	53, 501, 693, 962, 1085, 1696, 3916, 3934
BstSFI	CTRYAG	5 cut(s)	772, 954, 1063, 1551, 3292
BstSLI	GKGCMC	4 cut(s)	963, 1519, 1678, 3745
BstUI	CGCG	3 cut(s)	154, 1655, 1983
BstV1I	GCAGC	6 cut(s)	758, 786, 1309, 1792, 1975, 3000
BstV2I	GAAGAC	4 cut(s)	651, 2003, 3036, 3158
BstX2I	RGATCY	4 cut(s)	1819, 2937, 3613, 4010
BstXI	CCANNNNNNTGG	1 cut(s)	2078
BstYI	RGATCY	4 cut(s)	1819, 2937, 3613, 4010
BstZ17I	GTATAC	1 cut(s)	3711
Bsu15I	ATCGAT	2 cut(s)	1289, 2155
BsuI	GTATCC	4 cut(s)	921, 1714, 3150, 3934
BsuRI	GGCC	8 cut(s)	111, 1601, 1696, 2486, 3132, 3239, 3827, 3916
BsuTUI	ATCGAT	2 cut(s)	1289, 2155
BtgI	CCRYGG	3 cut(s)	106, 2287, 3186
BtgZI	GCGATG	2 cut(s)	150, 1143
BtsI	GCAGTG	1 cut(s)	963
BtsIMutI	CAGTG	7 cut(s)	856, 963, 1034, 1722, 1872, 2964, 4092
BtuMI	TCGCGA	1 cut(s)	154
BveI	ACCTGC	2 cut(s)	4, 652
Cac8I	GCNNGC	4 cut(s)	895, 954, 1803, 3134
CaiI	CAGNNNCTG	2 cut(s)	667, 2794
CciI	TCATGA	5 cut(s)	376, 577, 1363, 1942, 3067
CfoI	GCGC	3 cut(s)	1761, 1983, 3981
Cfr10I	RCCGGY	1 cut(s)	2174
Cfr13I	GGNCC	3 cut(s)	1375, 2360, 2549
ClaI	ATCGAT	2 cut(s)	1289, 2155
CseI	GACGC	1 cut(s)	1661
DraI	TTTAAA	4 cut(s)	1723, 1924, 3639, 4117
DraIII	CACNNNGTG	3 cut(s)	1040, 3732, 3902
DrdI	GACNNNNNNGTC	1 cut(s)	1373
DseDI	GACNNNNNNGTC	1 cut(s)	1373
EaeI	YGGCCR	6 cut(s)	109, 1599, 2484, 3130, 3237, 3825
Ecl136II	GAGCTC	2 cut(s)	1270, 3496
Eco130I	CCWWGG	6 cut(s)	106, 755, 1444, 2110, 2287, 3828
Eco24I	GRGCYC	3 cut(s)	1272, 3186, 3498
Eco31I	GGTCTC	1 cut(s)	191
Eco32I	GATATC	2 cut(s)	480, 2682
Eco47I	GGWCC	3 cut(s)	1375, 2360, 2549
Eco53kI	GAGCTC	2 cut(s)	1270, 3496
EcoICRI	GAGCTC	2 cut(s)	1270, 3496
EcoO109I	RGGNCCY	2 cut(s)	1375, 2360
EcoRI	GAATTC	1 cut(s)	2591
EcoRII	CCWGG	6 cut(s)	53, 693, 962, 1696, 3916, 3934
EcoRV	GATATC	2 cut(s)	480, 2682
EcoT14I	CCWWGG	6 cut(s)	106, 755, 1444, 2110, 2287, 3828
EcoT22I	ATGCAT	1 cut(s)	4241
EcoT38I	GRGCYC	3 cut(s)	1272, 3186, 3498
ErhI	CCWWGG	6 cut(s)	106, 755, 1444, 2110, 2287, 3828
FaqI	GGGAC	6 cut(s)	272, 485, 1455, 1789, 3681, 4009
FauI	CCCGC	2 cut(s)	886, 2875
FauNDI	CATATG	1 cut(s)	1843
FbaI	TGATCA	3 cut(s)	901, 1024, 3862
FblI	GTMKAC	4 cut(s)	399, 1062, 3291, 3710
Fnu4HI	GCNGC	7 cut(s)	671, 772, 775, 1298, 1806, 1964, 2989
FriOI	GRGCYC	3 cut(s)	1272, 3186, 3498
Fsp4HI	GCNGC	7 cut(s)	671, 772, 775, 1298, 1806, 1964, 2989
FspI	TGCGCA	2 cut(s)	1760, 3980
GlaI	GCGC	3 cut(s)	1760, 1982, 3980
GluI	GCNGC	7 cut(s)	671, 772, 775, 1298, 1806, 1964, 2989
GsaI	CCCAGC	2 cut(s)	2009, 3269
GsuI	CTGGAG	3 cut(s)	37, 1719, 2988
HaeIII	GGCC	8 cut(s)	111, 1601, 1696, 2486, 3132, 3239, 3827, 3916
HapII	CCGG	6 cut(s)	212, 443, 503, 1086, 2175, 3315
HgaI	GACGC	1 cut(s)	1661
HhaI	GCGC	3 cut(s)	1761, 1983, 3981
Hin6I	GCGC	3 cut(s)	1759, 1981, 3979
HinP1I	GCGC	3 cut(s)	1759, 1981, 3979
HincII	GTYRAC	4 cut(s)	400, 1827, 2200, 4047
HindII	GTYRAC	4 cut(s)	400, 1827, 2200, 4047
HindIII	AAGCTT	1 cut(s)	3500
HpaII	CCGG	6 cut(s)	212, 443, 503, 1086, 2175, 3315
HpyCH4III	ACNGT	5 cut(s)	1867, 3455, 4087, 4159, 4169
HpyCH4IV	ACGT	1 cut(s)	2202
HpyF10VI	GCNNNNNNNGC	7 cut(s)	132, 667, 894, 958, 3190, 3833, 4159
HpySE526I	ACGT	1 cut(s)	2202
HspAI	GCGC	3 cut(s)	1759, 1981, 3979
Kpn2I	TCCGGA	1 cut(s)	211
Ksp22I	TGATCA	3 cut(s)	901, 1024, 3862
LguI	GCTCTTC	2 cut(s)	942, 3174
LmnI	GCTCC	3 cut(s)	828, 1294, 3048
Lsp1109I	GCAGC	6 cut(s)	758, 786, 1309, 1792, 1975, 3000
LweI	GCATC	7 cut(s)	746, 1864, 2966, 3215, 3252, 4084, 4178
MaeII	ACGT	1 cut(s)	2202
MfeI	CAATTG	6 cut(s)	410, 1369, 1557, 2652, 3595, 3783
MflI	RGATCY	4 cut(s)	1819, 2937, 3613, 4010
MhlI	GDGCHC	7 cut(s)	963, 1272, 1519, 1678, 3186, 3498, 3745
MlsI	TGGCCA	3 cut(s)	1601, 3132, 3827
MluNI	TGGCCA	3 cut(s)	1601, 3132, 3827
MlyI	GAGTC	9 cut(s)	405, 562, 743, 775, 1477, 3026, 3074, 3140, 3703
MmeI	TCCRAC	6 cut(s)	1323, 1555, 1797, 3549, 3781, 4017
Mox20I	TGGCCA	3 cut(s)	1601, 3132, 3827
Mph1103I	ATGCAT	1 cut(s)	4241
MroI	TCCGGA	1 cut(s)	211
MroXI	GAANNNNTTC	6 cut(s)	37, 83, 2218, 2264, 3165, 3444
MscI	TGGCCA	3 cut(s)	1601, 3132, 3827
MslI	CAYNNNNRTG	3 cut(s)	2292, 2604, 3453
Msp20I	TGGCCA	3 cut(s)	1601, 3132, 3827
MspA1I	CMGCKG	4 cut(s)	907, 2870, 2991, 3136
MspI	CCGG	6 cut(s)	212, 443, 503, 1086, 2175, 3315
MspR9I	CCNGG	8 cut(s)	55, 503, 695, 964, 1087, 1698, 3918, 3936
MunI	CAATTG	6 cut(s)	410, 1369, 1557, 2652, 3595, 3783
Mva1269I	GAATGC	2 cut(s)	673, 3124
MvaI	CCWGG	6 cut(s)	55, 695, 964, 1698, 3918, 3936
MvnI	CGCG	3 cut(s)	154, 1655, 1983
MwoI	GCNNNNNNNGC	7 cut(s)	132, 667, 894, 958, 3190, 3833, 4159
NciI	CCSGG	2 cut(s)	503, 1087
NcoI	CCATGG	2 cut(s)	106, 2287
NdeI	CATATG	1 cut(s)	1843
NlaIV	GGNNCC	9 cut(s)	684, 1376, 1518, 2361, 2613, 2862, 3192, 3744, 4012
NmeAIII	GCCGAG	2 cut(s)	2462, 3265
NmuCI	GTSAC	7 cut(s)	452, 505, 1439, 1452, 1498, 3678, 3724
NruI	TCGCGA	1 cut(s)	154
NsbI	TGCGCA	2 cut(s)	1760, 3980
NsiI	ATGCAT	1 cut(s)	4241
NspI	RCATGY	5 cut(s)	884, 985, 1408, 3217, 4002
OliI	CACNNNNGTG	1 cut(s)	3453
PagI	TCATGA	5 cut(s)	376, 577, 1363, 1942, 3067
PasI	CCCWGGG	1 cut(s)	963
PciI	ACATGT	2 cut(s)	880, 3998
PciSI	GCTCTTC	2 cut(s)	942, 3174
PctI	GAATGC	2 cut(s)	673, 3124
PdmI	GAANNNNTTC	6 cut(s)	37, 83, 2218, 2264, 3165, 3444
PflFI	GACNNNGTC	2 cut(s)	571, 3083
PflMI	CCANNNNNTGG	2 cut(s)	2424, 3011
PfoI	TCCNGGA	1 cut(s)	53
PkrI	GCNGC	7 cut(s)	672, 773, 776, 1299, 1807, 1965, 2990
PleI	GAGTC	9 cut(s)	404, 562, 743, 775, 1476, 3026, 3074, 3140, 3702
PpsI	GAGTC	9 cut(s)	404, 562, 743, 775, 1476, 3026, 3074, 3140, 3702
PpuMI	RGGWCCY	2 cut(s)	1375, 2360
PscI	ACATGT	2 cut(s)	880, 3998
PsiI	TTATAA	1 cut(s)	2096
Psp124BI	GAGCTC	2 cut(s)	1272, 3498
Psp5II	RGGWCCY	2 cut(s)	1375, 2360
Psp6I	CCWGG	6 cut(s)	53, 693, 962, 1696, 3916, 3934
PspFI	CCCAGC	2 cut(s)	2005, 3265
PspGI	CCWGG	6 cut(s)	53, 693, 962, 1696, 3916, 3934
PspN4I	GGNNCC	9 cut(s)	684, 1376, 1518, 2361, 2613, 2862, 3192, 3744, 4012
PspPI	GGNCC	3 cut(s)	1375, 2360, 2549
PspPPI	RGGWCCY	2 cut(s)	1375, 2360
PsrI	GAACNNNNNNTAC	2 cut(s)	3877, 3909
PstI	CTGCAG	2 cut(s)	776, 958
PstNI	CAGNNNCTG	2 cut(s)	667, 2794
PsuI	RGATCY	4 cut(s)	1819, 2937, 3613, 4010
PsyI	GACNNNGTC	2 cut(s)	571, 3083
PvuII	CAGCTG	3 cut(s)	907, 2991, 3136
RruI	TCGCGA	1 cut(s)	154
RseI	CAYNNNNRTG	3 cut(s)	2292, 2604, 3453
SacI	GAGCTC	2 cut(s)	1272, 3498
SalI	GTCGAC	1 cut(s)	398
SapI	GCTCTTC	2 cut(s)	942, 3174
SatI	GCNGC	7 cut(s)	671, 772, 775, 1298, 1806, 1964, 2989
Sau96I	GGNCC	3 cut(s)	1375, 2360, 2549
ScaI	AGTACT	2 cut(s)	1013, 2231
SchI	GAGTC	9 cut(s)	405, 562, 743, 775, 1477, 3026, 3074, 3140, 3703
ScrFI	CCNGG	8 cut(s)	55, 503, 695, 964, 1087, 1698, 3918, 3936
SduI	GDGCHC	7 cut(s)	963, 1272, 1519, 1678, 3186, 3498, 3745
SfaNI	GCATC	7 cut(s)	746, 1864, 2966, 3215, 3252, 4084, 4178
SfcI	CTRYAG	5 cut(s)	772, 954, 1063, 1551, 3292
SgrAI	CRCCGGYG	1 cut(s)	2174
SinI	GGWCC	3 cut(s)	1375, 2360, 2549
SmiMI	CAYNNNNRTG	3 cut(s)	2292, 2604, 3453
SmlI	CTYRAG	3 cut(s)	1271, 1958, 3497
SmoI	CTYRAG	3 cut(s)	1271, 1958, 3497
SpeI	ACTAGT	1 cut(s)	1019
SsiI	CCGC	9 cut(s)	670, 893, 1543, 1858, 2120, 2165, 2292, 2868, 4078
SspI	AATATT	2 cut(s)	934, 3631
SstI	GAGCTC	2 cut(s)	1272, 3498
StyD4I	CCNGG	8 cut(s)	53, 501, 693, 962, 1085, 1696, 3916, 3934
StyI	CCWWGG	6 cut(s)	106, 755, 1444, 2110, 2287, 3828
TaaI	ACNGT	5 cut(s)	1867, 3455, 4087, 4159, 4169
TaiI	ACGT	1 cut(s)	2205
TaqI	TCGA	5 cut(s)	399, 779, 1289, 2155, 2184
TaqII	GACCGA	1 cut(s)	318
TatI	WGTACW	6 cut(s)	974, 1011, 1509, 2229, 3206, 4223
TauI	GCSGC	1 cut(s)	673
TscAI	CASTG	7 cut(s)	856, 963, 1041, 1722, 1872, 2971, 4092
TseFI	GTSAC	7 cut(s)	452, 505, 1439, 1452, 1498, 3678, 3724
TseI	GCWGC	6 cut(s)	771, 774, 1297, 1805, 1963, 2988
Tsp45I	GTSAC	7 cut(s)	452, 505, 1439, 1452, 1498, 3678, 3724
TspGWI	ACGGA	2 cut(s)	2604, 3524
TspRI	CASTG	7 cut(s)	856, 963, 1041, 1722, 1872, 2971, 4092
Tth111I	GACNNNGTC	2 cut(s)	571, 3083
Van91I	CCANNNNNTGG	2 cut(s)	2424, 3011
VneI	GTGCAC	1 cut(s)	1674
VpaK11BI	GGWCC	3 cut(s)	1375, 2360, 2549
XapI	RAATTY	8 cut(s)	62, 128, 550, 1766, 2132, 2591, 3986, 4230
XbaI	TCTAGA	1 cut(s)	2014
XceI	RCATGY	5 cut(s)	884, 985, 1408, 3217, 4002
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2294
XmiI	GTMKAC	4 cut(s)	399, 1062, 3291, 3710
XmnI	GAANNNNTTC	6 cut(s)	37, 83, 2218, 2264, 3165, 3444
ZrmI	AGTACT	2 cut(s)	1013, 2231
Zsp2I	ATGCAT	1 cut(s)	4241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.