RLG00000005251

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005232
Physical Location & Seq
Reverse (-)
66218932 .. 66220148
1217 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005251

Sequence Viewer

Length: 807 bp
ATGAGGGAAAAATTTGGCAAGTTTGTGGAAGTTATGGAGCCAGGATGCCATTGCATGCCTTGGTTTCTTGGGCATCAGGTTGCTGGTCATCTCTCTCTGCGTTTGCAGCAATTGGATGTGCGGTGTGAGACCAAGACAAAGGACAATGTCTTTGTCAATGTCGTTGCATCTATTCAATATCGTGCTCTGGCAGACAAAGCAAATGATGCATTCTACAAACTCAGCAACACTAGGTCTCAAATCCAGGCCTATGTTTTTGATGTGATTAGAGCCAGTGTTCCAAAGCTCCTTCTGGATGATGCTTTTGAGCAAAAGAATGAAATTGCAAAAGCTGTGGAAGATGAGCTTGAGAAGGCCATGTCCGCTTATGGTTATGAGATCGTACAAACTCTCATTGTTGACATAGAACCAGATGAGCATGTGAAACGTGCAATGAATGAAATCAATGCTGCTGCAAGAATGAGGGTGGCAGCTAATGAGAAGGCAGAGGCAGAGAAGATTTTGCAAATTAAACGGGCTGAGGGTGAGGCTGAGTCCAAGTATCTGTCTGGGCTGGGTATAGCTCGTCAACGTCAAGCAATTGTAGATGGTTTGAGAGACAGTGTACTGGGCTTTGCTGTTAATGTTCCAGGGACCACCGCAAAGGATGTCATGGACATGGTCCTTGTCACTCAGTATTTTGACACAATGAAGGAAATTGGTGCCAGCTCTAAATCCTCTGCTGTTTTTATTCCCCATGGACCTGGTGCCGTCCGCGATGTAGCTTCTCAGATCCGTGATGGACTTCTTCAGGGTTCTCATCAGTAG

Protein Analysis

269

Amino Acids

29.72

Weight (kDa)

5.92

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 3 - 166 2.4e-25 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 701, 746
AccII CGCG 1 cut(s) 756
AciI CCGC 4 cut(s) 121, 363, 639, 754
AclWI GGATC 1 cut(s) 766
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 773
AfaI GTAC 2 cut(s) 384, 606
AgsI TTSAA 1 cut(s) 176
AjnI CCWGG 4 cut(s) 40, 243, 628, 742
AluBI AGCT 7 cut(s) 286, 332, 346, 473, 563, 708, 764
AluI AGCT 7 cut(s) 286, 332, 346, 473, 563, 708, 764
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 3 cut(s) 122, 240, 591
AlwI GGATC 1 cut(s) 766
AoxI GGCC 2 cut(s) 246, 354
ApeKI GCWGC 4 cut(s) 106, 449, 452, 470
ApoI RAATTY 1 cut(s) 11
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspS9I GGNCC 3 cut(s) 633, 661, 740
AsuHPI GGTGA 1 cut(s) 536
AvaII GGWCC 3 cut(s) 633, 661, 740
BanI GGYRCC 2 cut(s) 701, 746
Bbv12I GWGCWC 1 cut(s) 187
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 118, 436, 439, 482
BccI CCATC 2 cut(s) 581, 773
BceAI ACGGC 1 cut(s) 734
BciT130I CCWGG 4 cut(s) 42, 245, 630, 744
BcoDI GTCTC 3 cut(s) 122, 240, 591
BfaI CTAG 1 cut(s) 231
BisI GCNGC 4 cut(s) 107, 450, 453, 471
BlsI GCNGC 4 cut(s) 108, 451, 454, 472
Bme1390I CCNGG 4 cut(s) 42, 245, 630, 744
Bme18I GGWCC 3 cut(s) 633, 661, 740
BmgT120I GGNCC 3 cut(s) 633, 661, 740
BmiI GGNNCC 4 cut(s) 39, 634, 703, 748
BmrFI CCNGG 4 cut(s) 42, 245, 630, 744
BmrI ACTGGG 1 cut(s) 617
BmsI GCATC 5 cut(s) 35, 82, 176, 196, 289
BmuI ACTGGG 1 cut(s) 617
Bpu10I CCTNAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 368
BsaI GGTCTC 2 cut(s) 122, 240
BsaJI CCNNGG 3 cut(s) 59, 629, 736
Bse1I ACTGG 2 cut(s) 273, 612
Bse3DI GCAATG 2 cut(s) 49, 438
BseBI CCWGG 4 cut(s) 42, 245, 630, 744
BseDI CCNNGG 3 cut(s) 59, 629, 736
BseGI GGATG 4 cut(s) 50, 121, 301, 652
BseMI GCAATG 2 cut(s) 49, 438
BseMII CTCAG 5 cut(s) 235, 510, 522, 686, 782
BseNI ACTGG 2 cut(s) 273, 612
BseXI GCAGC 4 cut(s) 118, 436, 439, 482
BseYI CCCAGC 1 cut(s) 553
Bsh1236I CGCG 1 cut(s) 756
BshFI GGCC 2 cut(s) 248, 356
BshNI GGYRCC 2 cut(s) 701, 746
BsiHKAI GWGCWC 1 cut(s) 187
BslFI GGGAC 1 cut(s) 646
BsmAI GTCTC 3 cut(s) 122, 240, 591
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 2 cut(s) 248, 356
Bso31I GGTCTC 2 cut(s) 122, 240
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 2 cut(s) 378, 771
Bsp19I CCATGG 1 cut(s) 736
BspACI CCGC 4 cut(s) 121, 363, 639, 754
BspANI GGCC 2 cut(s) 248, 356
BspCNI CTCAG 5 cut(s) 234, 511, 523, 685, 781
BspFNI CGCG 1 cut(s) 756
BspLI GGNNCC 4 cut(s) 39, 634, 703, 748
BspPI GGATC 1 cut(s) 766
BspT107I GGYRCC 2 cut(s) 701, 746
BspTNI GGTCTC 2 cut(s) 122, 240
BsrDI GCAATG 2 cut(s) 49, 438
BsrI ACTGG 2 cut(s) 273, 612
BssECI CCNNGG 3 cut(s) 59, 629, 736
BssMI GATC 2 cut(s) 378, 771
BssT1I CCWWGG 2 cut(s) 59, 736
Bst2UI CCWGG 4 cut(s) 42, 245, 630, 744
Bst4CI ACNGT 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 206
BstC8I GCNNGC 2 cut(s) 56, 706
BstDEI CTNAG 5 cut(s) 221, 519, 531, 672, 768
BstDSI CCRYGG 1 cut(s) 736
BstF5I GGATG 4 cut(s) 50, 121, 301, 652
BstFNI CGCG 1 cut(s) 756
BstKTI GATC 2 cut(s) 381, 774
BstMAI GTCTC 3 cut(s) 122, 240, 591
BstMBI GATC 2 cut(s) 378, 771
BstMWI GCNNNNNNNGC 4 cut(s) 106, 197, 206, 362
BstNI CCWGG 4 cut(s) 42, 245, 630, 744
BstNSI RCATGY 2 cut(s) 58, 422
BstSCI CCNGG 4 cut(s) 40, 243, 628, 742
BstUI CGCG 1 cut(s) 756
BstV1I GCAGC 4 cut(s) 118, 436, 439, 482
BstX2I RGATCY 1 cut(s) 771
BstXI CCANNNNNNTGG 1 cut(s) 743
BstYI RGATCY 1 cut(s) 771
BsuRI GGCC 2 cut(s) 248, 356
BtgI CCRYGG 1 cut(s) 736
BtgZI GCGATG 1 cut(s) 771
BtsCI GGATG 4 cut(s) 50, 121, 301, 652
BtsIMutI CAGTG 2 cut(s) 280, 607
Cac8I GCNNGC 2 cut(s) 56, 706
Cfr13I GGNCC 3 cut(s) 633, 661, 740
CsiI ACCWGGT 1 cut(s) 742
Csp6I GTAC 2 cut(s) 383, 605
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 6 cut(s) 55, 358, 419, 652, 658, 737
CviQI GTAC 2 cut(s) 383, 605
DdeI CTNAG 5 cut(s) 221, 519, 531, 672, 768
DpnI GATC 2 cut(s) 380, 773
DpnII GATC 2 cut(s) 378, 771
Eco130I CCWWGG 2 cut(s) 59, 736
Eco147I AGGCCT 1 cut(s) 248
Eco31I GGTCTC 2 cut(s) 122, 240
Eco47I GGWCC 3 cut(s) 633, 661, 740
Eco57I CTGAAG 1 cut(s) 773
EcoRII CCWGG 4 cut(s) 40, 243, 628, 742
EcoT14I CCWWGG 2 cut(s) 59, 736
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 2 cut(s) 59, 736
FaeI CATG 6 cut(s) 58, 361, 422, 655, 661, 740
FalI AAGNNNNNCTT 2 cut(s) 330, 362
FaqI GGGAC 1 cut(s) 646
FatI CATG 6 cut(s) 54, 357, 418, 651, 657, 736
Fnu4HI GCNGC 4 cut(s) 107, 450, 453, 471
FokI GGATG 4 cut(s) 57, 128, 308, 659
Fsp4HI GCNGC 4 cut(s) 107, 450, 453, 471
FspBI CTAG 1 cut(s) 231
GluI GCNGC 4 cut(s) 107, 450, 453, 471
GsaI CCCAGC 1 cut(s) 557
HaeIII GGCC 2 cut(s) 248, 356
Hin1II CATG 6 cut(s) 58, 361, 422, 655, 661, 740
HincII GTYRAC 2 cut(s) 400, 569
HindII GTYRAC 2 cut(s) 400, 569
HinfI GANTC 1 cut(s) 533
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 3 cut(s) 400, 569, 605
Hpy188I TCNGA 1 cut(s) 771
Hpy188III TCNNGA 1 cut(s) 293
Hpy8I GTNNAC 3 cut(s) 400, 569, 605
HpyAV CCTTC 4 cut(s) 299, 346, 475, 685
HpyCH4III ACNGT 1 cut(s) 602
HpyCH4IV ACGT 2 cut(s) 427, 571
HpyCH4V TGCA 8 cut(s) 54, 106, 167, 209, 326, 431, 455, 505
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 197, 206, 362
HpyF3I CTNAG 5 cut(s) 221, 519, 531, 672, 768
HpySE526I ACGT 2 cut(s) 427, 571
Hsp92II CATG 6 cut(s) 58, 361, 422, 655, 661, 740
Kzo9I GATC 2 cut(s) 378, 771
LmnI GCTCC 2 cut(s) 37, 291
Lsp1109I GCAGC 4 cut(s) 118, 436, 439, 482
LweI GCATC 5 cut(s) 35, 82, 176, 196, 289
MabI ACCWGGT 1 cut(s) 742
MaeI CTAG 1 cut(s) 231
MaeII ACGT 2 cut(s) 427, 571
MaeIII GTNAC 1 cut(s) 667
MalI GATC 2 cut(s) 380, 773
MboI GATC 2 cut(s) 378, 771
MboII GAAGA 3 cut(s) 350, 508, 779
MfeI CAATTG 2 cut(s) 110, 579
MflI RGATCY 1 cut(s) 771
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 6 cut(s) 11, 110, 321, 507, 579, 696
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 5 cut(s) 456, 481, 514, 520, 727
Mph1103I ATGCAT 1 cut(s) 211
MseI TTAA 2 cut(s) 510, 621
MslI CAYNNNNRTG 1 cut(s) 656
MspR9I CCNGG 4 cut(s) 42, 245, 630, 744
MunI CAATTG 2 cut(s) 110, 579
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 4 cut(s) 42, 245, 630, 744
MvnI CGCG 1 cut(s) 756
MwoI GCNNNNNNNGC 4 cut(s) 106, 197, 206, 362
NcoI CCATGG 1 cut(s) 736
NdeII GATC 2 cut(s) 378, 771
NlaIII CATG 6 cut(s) 58, 361, 422, 655, 661, 740
NlaIV GGNNCC 4 cut(s) 39, 634, 703, 748
NmuCI GTSAC 1 cut(s) 667
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 2 cut(s) 58, 422
PaeI GCATGC 1 cut(s) 58
PceI AGGCCT 1 cut(s) 248
PctI GAATGC 1 cut(s) 209
PflFI GACNNNGTC 2 cut(s) 146, 659
PkrI GCNGC 4 cut(s) 108, 451, 454, 472
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
Psp6I CCWGG 4 cut(s) 40, 243, 628, 742
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 4 cut(s) 40, 243, 628, 742
PspN4I GGNNCC 4 cut(s) 39, 634, 703, 748
PspPI GGNCC 3 cut(s) 633, 661, 740
PsuI RGATCY 1 cut(s) 771
PsyI GACNNNGTC 2 cut(s) 146, 659
RsaI GTAC 2 cut(s) 384, 606
RsaNI GTAC 2 cut(s) 383, 605
RseI CAYNNNNRTG 1 cut(s) 656
SaqAI TTAA 2 cut(s) 510, 621
SatI GCNGC 4 cut(s) 107, 450, 453, 471
Sau3AI GATC 2 cut(s) 378, 771
Sau96I GGNCC 3 cut(s) 633, 661, 740
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 4 cut(s) 42, 245, 630, 744
SduI GDGCHC 1 cut(s) 187
SexAI ACCWGGT 1 cut(s) 742
SfaNI GCATC 5 cut(s) 35, 82, 176, 196, 289
SinI GGWCC 3 cut(s) 633, 661, 740
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SphI GCATGC 1 cut(s) 58
Sse9I AATT 6 cut(s) 11, 110, 321, 507, 579, 696
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 4 cut(s) 121, 363, 639, 754
SspMI CTAG 1 cut(s) 231
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 4 cut(s) 40, 243, 628, 742
StyI CCWWGG 2 cut(s) 59, 736
TaaI ACNGT 1 cut(s) 602
TaiI ACGT 2 cut(s) 430, 574
TasI AATT 6 cut(s) 11, 110, 321, 507, 579, 696
TatI WGTACW 1 cut(s) 604
Tru1I TTAA 2 cut(s) 510, 621
Tru9I TTAA 2 cut(s) 510, 621
TscAI CASTG 2 cut(s) 280, 607
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 4 cut(s) 106, 449, 452, 470
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 4 cut(s) 333, 449, 453, 704
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 2 cut(s) 280, 607
Tth111I GACNNNGTC 2 cut(s) 146, 659
VpaK11BI GGWCC 3 cut(s) 633, 661, 740
XapI RAATTY 1 cut(s) 11
XceI RCATGY 2 cut(s) 58, 422
XspI CTAG 1 cut(s) 231
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.