Rorug06G0434500

Hypersensitive-induced response protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005232
Physical Location & Seq
Forward (+)
56962678 .. 56963463
786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0434500.1

Sequence Viewer

Length: 399 bp
ATGCTCTTCTCCATCTTCTTATTGCTTTTCCTACCCTGCGTAGGCATGAGCGCTGTGTTCGTGGTCTACATCTGTCTCTTATGGTTCGCCGCTAATACTAGGTCGTCGGCAGAGCCTCGGTTACCGGTCAAGCGGGTTTCCGAGAAGGGCTTGTCGGCCTCTGATCTCGACAAGTTGCCGAAAACTAGCGGCAAAGAGCTGACCAGGGGGACGGAGTGCGCGGTGTGCCTGGAGGAGATCGAGGCAGAGCAACCGGCTCGGGTGGTTCCAGGTTGTAACCACGGCTTCCATCTCCAATGCGCCGATACCTGGCTTTCTATGCAGTCATTCTGTCCGGTTTGTAGAGCCAAACTCCAACCTCAGCAGTTTCTCACTTCCTCTGATGAAAATCCATGCTAG

Protein Analysis

132

Amino Acids

14.6

Weight (kDa)

6.09

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 72 - 115 8e-14 Ring finger domain
zf-RING_11 PF17123 72 - 100 1.2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 72 - 114 2.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 73 - 115 4.3e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 73 - 114 5.8e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AccII CGCG 1 cut(s) 221
AciI CCGC 4 cut(s) 90, 133, 189, 221
AfeI AGCGCT 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 41, 309
AgeI ACCGGT 1 cut(s) 124
AjnI CCWGG 4 cut(s) 203, 228, 268, 308
AluBI AGCT 1 cut(s) 199
AluI AGCT 1 cut(s) 199
Alw26I GTCTC 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 258
Aor51HI AGCGCT 1 cut(s) 52
AoxI GGCC 1 cut(s) 156
AsiGI ACCGGT 1 cut(s) 124
AspLEI GCGC 3 cut(s) 53, 221, 302
AvaI CYCGRG 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 360
BccI CCATC 2 cut(s) 20, 297
BceAI ACGGC 1 cut(s) 298
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 4 cut(s) 205, 230, 270, 310
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 3 cut(s) 99, 186, 397
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 2 cut(s) 90, 190
BlsI GCNGC 2 cut(s) 91, 191
Bme1390I CCNGG 4 cut(s) 205, 230, 270, 310
BmeT110I CYCGRG 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 4 cut(s) 205, 230, 270, 310
BplI GAGNNNNNCTC 2 cut(s) 336, 368
BpmI CTGGAG 1 cut(s) 251
Bpu10I CCTNAGC 1 cut(s) 360
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 3 cut(s) 116, 204, 280
BsaWI WCCGGW 2 cut(s) 124, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 309
Bse118I RCCGGY 2 cut(s) 124, 253
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 4 cut(s) 205, 230, 270, 310
BseDI CCNNGG 3 cut(s) 116, 204, 280
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 41, 309
BseMII CTCAG 1 cut(s) 374
BseRI GAGGAG 1 cut(s) 248
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 3 cut(s) 125, 254, 335
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 2 cut(s) 41, 309
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 258
Bsp143I GATC 2 cut(s) 163, 237
BspACI CCGC 4 cut(s) 90, 133, 189, 221
BspANI GGCC 1 cut(s) 158
BspCNI CTCAG 1 cut(s) 373
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 267
BspQI GCTCTTC 1 cut(s) 11
BsrFI RCCGGY 2 cut(s) 124, 253
BssAI RCCGGY 2 cut(s) 124, 253
BssECI CCNNGG 3 cut(s) 116, 204, 280
BssMI GATC 2 cut(s) 163, 237
Bst2UI CCWGG 4 cut(s) 205, 230, 270, 310
Bst6I CTCTTC 1 cut(s) 11
BstDEI CTNAG 1 cut(s) 360
BstDSI CCRYGG 1 cut(s) 280
BstEII GGTNACC 1 cut(s) 120
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 221, 302
BstKTI GATC 2 cut(s) 166, 240
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 2 cut(s) 163, 237
BstMWI GCNNNNNNNGC 2 cut(s) 225, 319
BstNI CCWGG 4 cut(s) 205, 230, 270, 310
BstPI GGTNACC 1 cut(s) 120
BstSCI CCNGG 4 cut(s) 203, 228, 268, 308
BstUI CGCG 1 cut(s) 221
BsuRI GGCC 1 cut(s) 158
BtgI CCRYGG 1 cut(s) 280
CfoI GCGC 3 cut(s) 53, 221, 302
Cfr10I RCCGGY 2 cut(s) 124, 253
CspAI ACCGGT 1 cut(s) 124
CviAII CATG 2 cut(s) 46, 393
CviJI RGCY 8 cut(s) 115, 150, 158, 199, 257, 285, 313, 347
CviKI_1 RGCY 8 cut(s) 115, 150, 158, 199, 257, 285, 313, 347
DdeI CTNAG 1 cut(s) 360
DpnI GATC 2 cut(s) 165, 239
DpnII GATC 2 cut(s) 163, 237
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco47III AGCGCT 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 258
Eco91I GGTNACC 1 cut(s) 120
EcoO65I GGTNACC 1 cut(s) 120
EcoRII CCWGG 4 cut(s) 203, 228, 268, 308
FaeI CATG 2 cut(s) 49, 396
FaiI YATR 4 cut(s) 47, 82, 320, 394
FaqI GGGAC 1 cut(s) 223
FatI CATG 2 cut(s) 45, 392
FauI CCCGC 1 cut(s) 126
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 90, 190
Fsp4HI GCNGC 2 cut(s) 90, 190
FspBI CTAG 3 cut(s) 99, 186, 397
GlaI GCGC 3 cut(s) 52, 220, 301
GluI GCNGC 2 cut(s) 90, 190
GsuI CTGGAG 1 cut(s) 251
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 158
HapII CCGG 3 cut(s) 125, 254, 335
HhaI GCGC 3 cut(s) 53, 221, 302
Hin1II CATG 2 cut(s) 49, 396
Hin6I GCGC 3 cut(s) 51, 219, 300
HinP1I GCGC 3 cut(s) 51, 219, 300
HpaII CCGG 3 cut(s) 125, 254, 335
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 142, 163, 382
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 322
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 319
HpyF3I CTNAG 1 cut(s) 360
Hsp92II CATG 2 cut(s) 49, 396
HspAI GCGC 3 cut(s) 51, 219, 300
Kzo9I GATC 2 cut(s) 163, 237
LguI GCTCTTC 1 cut(s) 11
MaeI CTAG 3 cut(s) 99, 186, 397
MaeIII GTNAC 2 cut(s) 120, 275
MalI GATC 2 cut(s) 165, 239
MboI GATC 2 cut(s) 163, 237
MboII GAAGA 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 6 cut(s) 126, 169, 226, 235, 369, 388
MspI CCGG 3 cut(s) 125, 254, 335
MspR9I CCNGG 4 cut(s) 205, 230, 270, 310
MvaI CCWGG 4 cut(s) 205, 230, 270, 310
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 225, 319
NdeII GATC 2 cut(s) 163, 237
NlaIII CATG 2 cut(s) 49, 396
NlaIV GGNNCC 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 11
PinAI ACCGGT 1 cut(s) 124
PkrI GCNGC 2 cut(s) 91, 191
Psp6I CCWGG 4 cut(s) 203, 228, 268, 308
PspEI GGTNACC 1 cut(s) 120
PspGI CCWGG 4 cut(s) 203, 228, 268, 308
PspN4I GGNNCC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 11
SatI GCNGC 2 cut(s) 90, 190
Sau3AI GATC 2 cut(s) 163, 237
ScrFI CCNGG 4 cut(s) 205, 230, 270, 310
SetI ASST 5 cut(s) 104, 201, 274, 311, 361
SsiI CCGC 4 cut(s) 90, 133, 189, 221
SspMI CTAG 3 cut(s) 99, 186, 397
StyD4I CCNGG 4 cut(s) 203, 228, 268, 308
TaqI TCGA 2 cut(s) 168, 240
TauI GCSGC 2 cut(s) 92, 192
TspDTI ATGAA 1 cut(s) 399
TspGWI ACGGA 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 3 cut(s) 99, 186, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.