RLG00000009540

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016681
Physical Location & Seq
Reverse (-)
54240571 .. 54244561
3991 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009540

Sequence Viewer

Length: 1065 bp
ATGTGGAAGTTTCTACAGTCCTTCCTTGTAGGGATACCTTGTTACAGCATCCAAGAATCAGATCTTGTGATCTTCTTTCCAAAATGTCTTCTTCAGTTGCCCTCGACTCCGGATGGTTATATGCAGATTCTTATGGATGAGTGGAATATAAAACCTCCTAGTTGGGTGAGTACCGATCTGACGGCTGGGAAGGGCCTGACAGGACAACTTCAAGCTTCAATTGGAAAATTGACGAAACTAGTACACTTAAACCTGGTTGGTTGCAGTTTCTTTGGTCCTATTCCAGATTCTAACGAAGCTGTCTTACCTGTAAGATGTCTGGTCTTTCTATTCCTTAGCGCTTTTTTTTTTTTTTGGCTAATATGCAAATTATGGCAATCTTCATTGGTTTTCTACTGCAGATCTCTGAAAACCAATAGTTTCAGTGGGCCAATTCTGCCTTCCATTGGTAATCTGGCCAATCTTACTTTTATAGACCTATCTGACAACAAGCTTCAGGGATCCATCCCAGTCTTTAATGGGACTACACTTGGTCTTGATAAGTTACTTAAGTCGAGACACTTTCATTTTGGGAACAATCAGCTCTCGGGCCCAATTCCACCTCGACTTTTCAACTCAACGATGGCCATGATACATTTACGCCTGGAAGGAAATTCATTAAGTGGGCGTGTGCCTTCGAGCCTCAACAACCTTACTAATGTGTCTGAACTGCACTTGTCCAATAATATGCTGACTGGCCCTATGCCCAACCTTACTGGCATGAATTCCCTCAACTATGTGGATTTGAGCAACAATACTTTTGCTAAGTCGGAGGTTCCTACCTGGTTTTCTACCTTGAAGCTTTTGACAACATTAATGATGGAAAACACGGGGCTTCCGGGAGAAGTTCCTCGTGCCCTCTTCAGCTTTGGGAATTTGGAGACAGTGGTACTGCGGAACAACCTTCTCAGTGGCTTATTAGATGTTGGAAACTCCAGCAACCAGTTGCAACTAATTGATCTGCAGCGGAACTTCATTACTGAACTTGCACCAAACAAGGAAGGATCCAACTACACATTGAAGTAG

Protein Analysis

355

Amino Acids

39.25

Weight (kDa)

8.18

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016681)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74200 AT1G74200 AT1G74200
malus_domestica MD10G1178000.v1.1
rosa_chinensis RchiOBHm_Chr2g0137671
rosa_laevigata RLG00000009540 RLG00000023880
rosa_roxburghii Rroxscaffold_4G00287120
rosa_samantha Rh2AG359700 Rh2AG462200 Rh4DG211200 Rh4DG239300 Rh7AG276800
rosa_wichuraiana Rw4G018920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 109
AciI CCGC 2 cut(s) 934, 1006
AclWI GGATC 4 cut(s) 495, 508, 1038, 1051
AcoI YGGCCR 2 cut(s) 456, 624
AcsI RAATTY 3 cut(s) 652, 763, 913
AcuI CTGAAG 3 cut(s) 77, 479, 886
AfaI GTAC 3 cut(s) 172, 243, 930
AfeI AGCGCT 1 cut(s) 340
AfiI CCNNNNNNNGG 1 cut(s) 446
AflII CTTAAG 1 cut(s) 548
AgsI TTSAA 5 cut(s) 212, 219, 613, 838, 1060
AhlI ACTAGT 1 cut(s) 238
AjnI CCWGG 3 cut(s) 252, 642, 821
AjuI GAANNNNNNNTTGG 2 cut(s) 424, 456
AluBI AGCT 6 cut(s) 215, 299, 493, 583, 841, 906
AluI AGCT 6 cut(s) 215, 299, 493, 583, 841, 906
Alw26I GTCTC 2 cut(s) 550, 914
AlwI GGATC 4 cut(s) 495, 508, 1038, 1051
Ama87I CYCGRG 1 cut(s) 586
Aor13HI TCCGGA 1 cut(s) 109
Aor51HI AGCGCT 1 cut(s) 340
AoxI GGCC 6 cut(s) 193, 428, 456, 589, 624, 736
ApaI GGGCCC 1 cut(s) 593
ApeKI GCWGC 1 cut(s) 1003
ApoI RAATTY 3 cut(s) 652, 763, 913
AseI ATTAAT 1 cut(s) 854
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 6 cut(s) 193, 275, 428, 589, 590, 737
AsuC2I CCSGG 1 cut(s) 879
AsuHPI GGTGA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 1 cut(s) 275
BaeGI GKGCMC 2 cut(s) 593, 898
BalI TGGCCA 2 cut(s) 458, 626
BamHI GGATCC 2 cut(s) 500, 1043
BanII GRGCYC 1 cut(s) 593
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 1015
BccI CCATC 4 cut(s) 107, 512, 616, 853
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 3 cut(s) 254, 644, 823
BciVI GTATCC 1 cut(s) 27
BcnI CCSGG 1 cut(s) 879
BcoDI GTCTC 2 cut(s) 550, 914
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 2 cut(s) 159, 239
BfmI CTRYAG 3 cut(s) 14, 397, 1001
BfoI RGCGCY 1 cut(s) 342
BfrI CTTAAG 1 cut(s) 548
BfuI GTATCC 1 cut(s) 27
BglII AGATCT 2 cut(s) 61, 401
BisI GCNGC 1 cut(s) 1004
BlsI GCNGC 1 cut(s) 1005
Bme1390I CCNGG 4 cut(s) 254, 644, 823, 879
Bme18I GGWCC 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 6 cut(s) 193, 275, 428, 589, 590, 737
BmiI GGNNCC 4 cut(s) 502, 591, 816, 1045
BmrFI CCNGG 4 cut(s) 254, 644, 823, 879
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 1 cut(s) 57
BmuI ACTGGG 1 cut(s) 503
BpiI GAAGAC 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 958
Bpu10I CCTNAGC 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 879
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 446
Bse1I ACTGG 4 cut(s) 509, 739, 760, 982
BseAI TCCGGA 1 cut(s) 109
BseBI CCWGG 3 cut(s) 254, 644, 823
BseGI GGATG 4 cut(s) 48, 118, 142, 504
BseLI CCNNNNNNNGG 1 cut(s) 446
BseMII CTCAG 1 cut(s) 961
BseNI ACTGG 4 cut(s) 509, 739, 760, 982
BseSI GKGCMC 2 cut(s) 593, 898
BseXI GCAGC 1 cut(s) 1015
BseYI CCCAGC 1 cut(s) 185
BsgI GTGCAG 1 cut(s) 695
BshFI GGCC 6 cut(s) 195, 430, 458, 591, 626, 738
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 2 cut(s) 110, 878
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 1 cut(s) 446
BsmAI GTCTC 2 cut(s) 550, 914
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 6 cut(s) 195, 430, 458, 591, 626, 738
BsoBI CYCGRG 1 cut(s) 586
Bsp120I GGGCCC 1 cut(s) 589
Bsp1286I GDGCHC 2 cut(s) 593, 898
Bsp13I TCCGGA 1 cut(s) 109
Bsp143I GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
BspACI CCGC 2 cut(s) 934, 1006
BspANI GGCC 6 cut(s) 195, 430, 458, 591, 626, 738
BspCNI CTCAG 1 cut(s) 960
BspEI TCCGGA 1 cut(s) 109
BspLI GGNNCC 4 cut(s) 502, 591, 816, 1045
BspMAI CTGCAG 2 cut(s) 401, 1005
BspPI GGATC 4 cut(s) 495, 508, 1038, 1051
BspTI CTTAAG 1 cut(s) 548
BsrI ACTGG 4 cut(s) 509, 739, 760, 982
BssMI GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
BssSI CACGAG 1 cut(s) 891
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 3 cut(s) 254, 644, 823
Bst4CI ACNGT 2 cut(s) 18, 925
Bst6I CTCTTC 1 cut(s) 905
BstAFI CTTAAG 1 cut(s) 548
BstDEI CTNAG 3 cut(s) 335, 804, 947
BstF5I GGATG 4 cut(s) 48, 118, 142, 504
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 7 cut(s) 64, 72, 178, 404, 503, 1000, 1046
BstMAI GTCTC 2 cut(s) 550, 914
BstMBI GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
BstMWI GCNNNNNNNGC 1 cut(s) 436
BstNI CCWGG 3 cut(s) 254, 644, 823
BstSCI CCNGG 4 cut(s) 252, 642, 821, 877
BstSFI CTRYAG 3 cut(s) 14, 397, 1001
BstSLI GKGCMC 2 cut(s) 593, 898
BstV1I GCAGC 1 cut(s) 1015
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 4 cut(s) 61, 401, 500, 1043
BstYI RGATCY 4 cut(s) 61, 401, 500, 1043
BsuI GTATCC 1 cut(s) 27
BsuRI GGCC 6 cut(s) 195, 430, 458, 591, 626, 738
BtsCI GGATG 4 cut(s) 48, 118, 142, 504
BtsIMutI CAGTG 3 cut(s) 430, 930, 955
CfoI GCGC 1 cut(s) 341
Cfr13I GGNCC 6 cut(s) 193, 275, 428, 589, 590, 737
CsiI ACCWGGT 2 cut(s) 252, 821
Csp6I GTAC 3 cut(s) 171, 242, 929
CviAII CATG 2 cut(s) 628, 760
CviQI GTAC 3 cut(s) 171, 242, 929
DdeI CTNAG 3 cut(s) 335, 804, 947
DpnI GATC 7 cut(s) 63, 71, 177, 403, 502, 999, 1045
DpnII GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
EaeI YGGCCR 2 cut(s) 456, 624
Eam1104I CTCTTC 1 cut(s) 905
EarI CTCTTC 1 cut(s) 905
Eco24I GRGCYC 1 cut(s) 593
Eco47I GGWCC 1 cut(s) 275
Eco47III AGCGCT 1 cut(s) 340
Eco57I CTGAAG 3 cut(s) 77, 479, 886
Eco88I CYCGRG 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 193
EcoRI GAATTC 1 cut(s) 763
EcoRII CCWGG 3 cut(s) 252, 642, 821
EcoT38I GRGCYC 1 cut(s) 593
FaeI CATG 2 cut(s) 631, 763
FaqI GGGAC 1 cut(s) 535
FatI CATG 2 cut(s) 627, 759
Fnu4HI GCNGC 1 cut(s) 1004
FokI GGATG 4 cut(s) 35, 125, 149, 491
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 1 cut(s) 1004
FspBI CTAG 2 cut(s) 159, 239
GlaI GCGC 1 cut(s) 340
GluI GCNGC 1 cut(s) 1004
GsaI CCCAGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 958
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 6 cut(s) 195, 430, 458, 591, 626, 738
HapII CCGG 2 cut(s) 110, 878
HhaI GCGC 1 cut(s) 341
Hin1II CATG 2 cut(s) 631, 763
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HindIII AAGCTT 3 cut(s) 213, 491, 839
HinfI GANTC 4 cut(s) 56, 106, 127, 287
HpaII CCGG 2 cut(s) 110, 878
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 6 cut(s) 61, 180, 408, 484, 706, 811
Hpy188III TCNNGA 4 cut(s) 110, 284, 536, 555
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 7 cut(s) 31, 184, 450, 641, 684, 953, 1034
HpyCH4III ACNGT 2 cut(s) 18, 925
HpyCH4V TGCA 8 cut(s) 124, 264, 366, 399, 712, 988, 1003, 1028
HpyF10VI GCNNNNNNNGC 1 cut(s) 436
HpyF3I CTNAG 3 cut(s) 335, 804, 947
Hsp92II CATG 2 cut(s) 631, 763
HspAI GCGC 1 cut(s) 339
Kpn2I TCCGGA 1 cut(s) 109
Kzo9I GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
Lsp1109I GCAGC 1 cut(s) 1015
LweI GCATC 1 cut(s) 57
MabI ACCWGGT 2 cut(s) 252, 821
MaeI CTAG 2 cut(s) 159, 239
MaeIII GTNAC 2 cut(s) 41, 543
MalI GATC 7 cut(s) 63, 71, 177, 403, 502, 999, 1045
MboI GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
MboII GAAGA 5 cut(s) 64, 80, 83, 372, 892
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 4 cut(s) 61, 401, 500, 1043
MhlI GDGCHC 2 cut(s) 593, 898
MlsI TGGCCA 2 cut(s) 458, 626
MluCI AATT 9 cut(s) 219, 227, 368, 432, 594, 652, 763, 913, 993
MluNI TGGCCA 2 cut(s) 458, 626
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 2 cut(s) 789, 946
MnlI CCTC 8 cut(s) 112, 165, 612, 692, 779, 805, 900, 908
Mox20I TGGCCA 2 cut(s) 458, 626
MroI TCCGGA 1 cut(s) 109
MscI TGGCCA 2 cut(s) 458, 626
MseI TTAA 5 cut(s) 248, 516, 549, 659, 854
Msp20I TGGCCA 2 cut(s) 458, 626
MspA1I CMGCKG 1 cut(s) 1006
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 2 cut(s) 110, 878
MspR9I CCNGG 4 cut(s) 254, 644, 823, 879
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 3 cut(s) 254, 644, 823
MwoI GCNNNNNNNGC 1 cut(s) 436
NciI CCSGG 1 cut(s) 879
NdeII GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
NlaIII CATG 2 cut(s) 631, 763
NlaIV GGNNCC 4 cut(s) 502, 591, 816, 1045
PfeI GAWTC 3 cut(s) 56, 127, 287
PfoI TCCNGGA 1 cut(s) 877
PkrI GCNGC 1 cut(s) 1005
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PshBI ATTAAT 1 cut(s) 854
Psp6I CCWGG 3 cut(s) 252, 642, 821
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 3 cut(s) 252, 642, 821
PspN4I GGNNCC 4 cut(s) 502, 591, 816, 1045
PspOMI GGGCCC 1 cut(s) 589
PspPI GGNCC 6 cut(s) 193, 275, 428, 589, 590, 737
PstI CTGCAG 2 cut(s) 401, 1005
PsuI RGATCY 4 cut(s) 61, 401, 500, 1043
RsaI GTAC 3 cut(s) 172, 243, 930
RsaNI GTAC 3 cut(s) 171, 242, 929
SaqAI TTAA 5 cut(s) 248, 516, 549, 659, 854
SatI GCNGC 1 cut(s) 1004
Sau3AI GATC 7 cut(s) 61, 69, 175, 401, 500, 997, 1043
Sau96I GGNCC 6 cut(s) 193, 275, 428, 589, 590, 737
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 4 cut(s) 254, 644, 823, 879
SduI GDGCHC 2 cut(s) 593, 898
SexAI ACCWGGT 2 cut(s) 252, 821
SfaNI GCATC 1 cut(s) 57
SfcI CTRYAG 3 cut(s) 14, 397, 1001
SinI GGWCC 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 9 cut(s) 219, 227, 368, 432, 594, 652, 763, 913, 993
SsiI CCGC 2 cut(s) 934, 1006
SspMI CTAG 2 cut(s) 159, 239
StyD4I CCNGG 4 cut(s) 252, 642, 821, 877
TaaI ACNGT 2 cut(s) 18, 925
TaqI TCGA 4 cut(s) 104, 554, 604, 677
TasI AATT 9 cut(s) 219, 227, 368, 432, 594, 652, 763, 913, 993
TatI WGTACW 1 cut(s) 241
TfiI GAWTC 3 cut(s) 56, 127, 287
Tru1I TTAA 5 cut(s) 248, 516, 549, 659, 854
Tru9I TTAA 5 cut(s) 248, 516, 549, 659, 854
TscAI CASTG 3 cut(s) 430, 930, 955
TseI GCWGC 1 cut(s) 1003
TspDTI ATGAA 5 cut(s) 372, 554, 645, 776, 1003
TspRI CASTG 3 cut(s) 430, 930, 955
Vha464I CTTAAG 1 cut(s) 548
VpaK11BI GGWCC 1 cut(s) 275
VspI ATTAAT 1 cut(s) 854
XapI RAATTY 3 cut(s) 652, 763, 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XspI CTAG 2 cut(s) 159, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.