Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0016275

Physical Location & Seq

Genomic Coordinates Forward (+)

4074743 .. 4075504

Gene Structure 762 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010748

Sequence Viewer

CDS Protein Promoter (2k)

Length: 762 bp

ATGGATGCAAATATTGCCAATATGTGCTCTTCTTCTACTTCTACCTCCGACTCTTCTTCCTCAGAGTCCTCGCAGCCCAGAAATCCCAACAAGGCCGAGAGAATCAAGGGCCCGTGGAGCGCCGAGGAGGATCGGGTTCTCACCCGCCTCGTCGAGCGCTACGGCCCCAGAAATTGGTCTTTGATTAGCCGCTACATTAAGGGTCGCTCGGGCAAGTCCTGCAGGCTTAGGTGGTGCAACCAGCTCAGCCCCAGCGTCCAGCACCGTCCCTTTTCCCAAGCCGAAGACGAAACCATCCTCGCCGCGCACTCCCGCTACGGCAACCGGTGGGCCACCATTGCCCGGTTGCTCCCGGGCCGGACCGACAATGCGGTCAAGAACCACTGGAACTCGACCCTGAAGAGAAGGGTAAGAGAACCACAAGACCACCAAGAATTGGATCATCAGGGCGAGGGTAATATTGGCAATGCAGCGATTGAGGTGATGATGATGGGTAATACTACTACCACTACTACTAGTGCTTCAGCTGCCTCCGGGCGTAATGGGTCCATGGATCTGGATGACCCGTTGACCGAGTTGACTCTGGCCACGCCGGGGATTGGTGGCTCTGTGGTGTCGTCGTCGGAACAACACCACCATCACCGGAGCAGCAGCGAGAGTTTTCCTCCGGCGGGGTTTTGGGATGTGATGAGGGGTGTGATTGCCAGGGAAGTTAGGGATTACGTGAGCTCGTCATTTTCAGAAACTTCTGGGCTTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009751 GO:0009753 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0046686 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000022 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

254

Amino Acids

27.92

Weight (kDa)

7.85

Isoelectric Point (pI)

55.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	36 - 82	6.2e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	39 - 98	8.7e-18	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	89 - 132	1.4e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	91 - 145	2.8e-10	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016275)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35010
malus_domestica	MD08G1107400.v1.1 MD15G1088000.v1.1
prunus_persica	Prupe.1G441700_v2.0.a1
pyrus_communis	pycom08g08930 pycom15g08260
rosa_chinensis	RchiOBHm_Chr6g0307001
rosa_laevigata	RLG00000010748
rosa_roxburghii	Rroxscaffold_7G00161290
rosa_rugosa	Rorug06G0354900
rosa_samantha	Rh6AG466700 Rh6BG434300 Rh6CG481700 Rh6DG468000
rosa_wichuraiana	Rw6G040660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	371
AccB7I	CCANNNNNTGG	2 cut(s)	174, 436
AccII	CGCG	1 cut(s)	305
AciI	CCGC	6 cut(s)	145, 190, 303, 313, 371, 671
AclWI	GGATC	3 cut(s)	138, 447, 561
AcoI	YGGCCR	1 cut(s)	585
AcuI	CTGAAG	2 cut(s)	419, 507
AfeI	AGCGCT	1 cut(s)	158
AfiI	CCNNNNNNNGG	6 cut(s)	174, 342, 436, 594, 599, 671
AgeI	ACCGGT	1 cut(s)	324
AhlI	ACTAGT	1 cut(s)	515
AjnI	CCWGG	1 cut(s)	704
AluBI	AGCT	3 cut(s)	244, 527, 729
AluI	AGCT	3 cut(s)	244, 527, 729
Alw21I	GWGCWC	2 cut(s)	29, 731
AlwI	GGATC	3 cut(s)	138, 447, 561
Ama87I	CYCGRG	2 cut(s)	208, 352
Aor51HI	AGCGCT	1 cut(s)	158
AoxI	GGCC	6 cut(s)	93, 109, 163, 330, 355, 585
ApaI	GGGCCC	1 cut(s)	113
ApeKI	GCWGC	5 cut(s)	73, 470, 527, 648, 651
AsiGI	ACCGGT	1 cut(s)	324
AspLEI	GCGC	3 cut(s)	122, 159, 307
AspS9I	GGNCC	7 cut(s)	109, 110, 164, 330, 355, 360, 546
AsuC2I	CCSGG	5 cut(s)	343, 353, 354, 535, 594
AsuHPI	GGTGA	3 cut(s)	133, 493, 632
AvaI	CYCGRG	2 cut(s)	208, 352
AvaII	GGWCC	2 cut(s)	360, 546
BaeGI	GKGCMC	1 cut(s)	113
BalI	TGGCCA	1 cut(s)	587
BanII	GRGCYC	2 cut(s)	113, 731
BbsI	GAAGAC	1 cut(s)	291
Bbv12I	GWGCWC	2 cut(s)	29, 731
BbvI	GCAGC	5 cut(s)	85, 482, 514, 660, 663
BccI	CCATC	3 cut(s)	302, 484, 645
BceAI	ACGGC	2 cut(s)	178, 334
BciT130I	CCWGG	1 cut(s)	706
BcnI	CCSGG	5 cut(s)	343, 353, 354, 535, 594
BcuI	ACTAGT	1 cut(s)	515
BfaI	CTAG	1 cut(s)	516
BfmI	CTRYAG	1 cut(s)	220
BfoI	RGCGCY	2 cut(s)	123, 160
BisI	GCNGC	7 cut(s)	74, 190, 303, 471, 528, 649, 652
BlpI	GCTNAGC	1 cut(s)	245
BlsI	GCNGC	7 cut(s)	75, 191, 304, 472, 529, 650, 653
Bme1390I	CCNGG	6 cut(s)	343, 353, 354, 535, 594, 706
Bme18I	GGWCC	2 cut(s)	360, 546
BmeT110I	CYCGRG	2 cut(s)	208, 352
BmgT120I	GGNCC	7 cut(s)	109, 110, 164, 330, 355, 360, 546
BmiI	GGNNCC	3 cut(s)	111, 166, 547
BmrFI	CCNGG	6 cut(s)	343, 353, 354, 535, 594, 706
BpiI	GAAGAC	1 cut(s)	291
BplI	GAGNNNNNCTC	2 cut(s)	649, 681
Bpu10I	CCTNAGC	1 cut(s)	227
Bpu1102I	GCTNAGC	1 cut(s)	245
BpuMI	CCSGG	5 cut(s)	343, 353, 354, 535, 594
BsaAI	YACGTR	1 cut(s)	724
BsaJI	CCNNGG	6 cut(s)	113, 123, 352, 549, 593, 705
BsaWI	WCCGGW	2 cut(s)	324, 642
Bsc4I	CCNNNNNNNGG	6 cut(s)	174, 342, 436, 594, 599, 671
Bse118I	RCCGGY	1 cut(s)	324
Bse1I	ACTGG	1 cut(s)	389
Bse3DI	GCAATG	2 cut(s)	336, 472
BseBI	CCWGG	1 cut(s)	706
BseDI	CCNNGG	6 cut(s)	113, 123, 352, 549, 593, 705
BseGI	GGATG	4 cut(s)	10, 294, 565, 688
BseLI	CCNNNNNNNGG	6 cut(s)	174, 342, 436, 594, 599, 671
BseMI	GCAATG	2 cut(s)	336, 472
BseMII	CTCAG	2 cut(s)	75, 259
BseNI	ACTGG	1 cut(s)	389
BseRI	GAGGAG	1 cut(s)	140
BseSI	GKGCMC	1 cut(s)	113
BseXI	GCAGC	5 cut(s)	85, 482, 514, 660, 663
BseYI	CCCAGC	1 cut(s)	251
Bsh1236I	CGCG	1 cut(s)	305
BshFI	GGCC	6 cut(s)	95, 111, 165, 332, 357, 587
BshTI	ACCGGT	1 cut(s)	324
BsiHKAI	GWGCWC	2 cut(s)	29, 731
BsiHKCI	CYCGRG	2 cut(s)	208, 352
BsiSI	CCGG	8 cut(s)	325, 343, 353, 358, 534, 593, 643, 668
BslFI	GGGAC	1 cut(s)	252
BslI	CCNNNNNNNGG	6 cut(s)	174, 342, 436, 594, 599, 671
BsmFI	GGGAC	1 cut(s)	252
BsnI	GGCC	6 cut(s)	95, 111, 165, 332, 357, 587
BsoBI	CYCGRG	2 cut(s)	208, 352
Bsp120I	GGGCCC	1 cut(s)	109
Bsp1286I	GDGCHC	3 cut(s)	29, 113, 731
Bsp143I	GATC	3 cut(s)	130, 439, 553
Bsp1720I	GCTNAGC	1 cut(s)	245
Bsp19I	CCATGG	1 cut(s)	549
BspACI	CCGC	6 cut(s)	145, 190, 303, 313, 371, 671
BspANI	GGCC	6 cut(s)	95, 111, 165, 332, 357, 587
BspCNI	CTCAG	2 cut(s)	74, 258
BspFNI	CGCG	1 cut(s)	305
BspLI	GGNNCC	3 cut(s)	111, 166, 547
BspMAI	CTGCAG	1 cut(s)	224
BspPI	GGATC	3 cut(s)	138, 447, 561
BspQI	GCTCTTC	1 cut(s)	34
BsrDI	GCAATG	2 cut(s)	336, 472
BsrFI	RCCGGY	1 cut(s)	324
BsrI	ACTGG	1 cut(s)	389
BssAI	RCCGGY	1 cut(s)	324
BssECI	CCNNGG	6 cut(s)	113, 123, 352, 549, 593, 705
BssMI	GATC	3 cut(s)	130, 439, 553
BssT1I	CCWWGG	1 cut(s)	549
Bst2UI	CCWGG	1 cut(s)	706
Bst4CI	ACNGT	1 cut(s)	266
Bst6I	CTCTTC	3 cut(s)	34, 58, 395
BstAPI	GCANNNNNTGC	2 cut(s)	14, 219
BstBAI	YACGTR	1 cut(s)	724
BstC8I	GCNNGC	1 cut(s)	224
BstDEI	CTNAG	3 cut(s)	61, 227, 245
BstDSI	CCRYGG	2 cut(s)	113, 549
BstF5I	GGATG	4 cut(s)	10, 294, 565, 688
BstFNI	CGCG	1 cut(s)	305
BstH2I	RGCGCY	2 cut(s)	123, 160
BstHHI	GCGC	3 cut(s)	122, 159, 307
BstKTI	GATC	3 cut(s)	133, 442, 556
BstMBI	GATC	3 cut(s)	130, 439, 553
BstMWI	GCNNNNNNNGC	5 cut(s)	14, 117, 219, 338, 527
BstNI	CCWGG	1 cut(s)	706
BstSCI	CCNGG	6 cut(s)	341, 351, 352, 533, 592, 704
BstSFI	CTRYAG	1 cut(s)	220
BstSLI	GKGCMC	1 cut(s)	113
BstUI	CGCG	1 cut(s)	305
BstV1I	GCAGC	5 cut(s)	85, 482, 514, 660, 663
BstV2I	GAAGAC	1 cut(s)	291
BstX2I	RGATCY	1 cut(s)	553
BstXI	CCANNNNNNTGG	1 cut(s)	556
BstYI	RGATCY	1 cut(s)	553
BsuRI	GGCC	6 cut(s)	95, 111, 165, 332, 357, 587
BtgI	CCRYGG	2 cut(s)	113, 549
BtsCI	GGATG	4 cut(s)	10, 294, 565, 688
BtsIMutI	CAGTG	1 cut(s)	382
Cac8I	GCNNGC	1 cut(s)	224
CfoI	GCGC	3 cut(s)	122, 159, 307
Cfr10I	RCCGGY	1 cut(s)	324
Cfr13I	GGNCC	7 cut(s)	109, 110, 164, 330, 355, 360, 546
Cfr9I	CCCGGG	1 cut(s)	352
CpoI	CGGWCCG	1 cut(s)	360
CseI	GACGC	1 cut(s)	244
CspAI	ACCGGT	1 cut(s)	324
CspI	CGGWCCG	1 cut(s)	360
CviAII	CATG	1 cut(s)	550
DdeI	CTNAG	3 cut(s)	61, 227, 245
DpnI	GATC	3 cut(s)	132, 441, 555
DpnII	GATC	3 cut(s)	130, 439, 553
DrdI	GACNNNNNNGTC	1 cut(s)	371
DseDI	GACNNNNNNGTC	1 cut(s)	371
EaeI	YGGCCR	1 cut(s)	585
Eam1104I	CTCTTC	3 cut(s)	34, 58, 395
EarI	CTCTTC	3 cut(s)	34, 58, 395
Ecl136II	GAGCTC	1 cut(s)	729
Eco130I	CCWWGG	1 cut(s)	549
Eco24I	GRGCYC	2 cut(s)	113, 731
Eco47I	GGWCC	2 cut(s)	360, 546
Eco47III	AGCGCT	1 cut(s)	158
Eco53kI	GAGCTC	1 cut(s)	729
Eco57I	CTGAAG	2 cut(s)	419, 507
Eco88I	CYCGRG	2 cut(s)	208, 352
EcoICRI	GAGCTC	1 cut(s)	729
EcoO109I	RGGNCCY	1 cut(s)	109
EcoRII	CCWGG	1 cut(s)	704
EcoT14I	CCWWGG	1 cut(s)	549
EcoT38I	GRGCYC	2 cut(s)	113, 731
ErhI	CCWWGG	1 cut(s)	549
FaeI	CATG	1 cut(s)	553
FaiI	YATR	2 cut(s)	23, 551
FaqI	GGGAC	1 cut(s)	252
FatI	CATG	1 cut(s)	549
FauI	CCCGC	3 cut(s)	152, 320, 664
Fnu4HI	GCNGC	7 cut(s)	74, 190, 303, 471, 528, 649, 652
FokI	GGATG	4 cut(s)	17, 281, 572, 695
FriOI	GRGCYC	2 cut(s)	113, 731
Fsp4HI	GCNGC	7 cut(s)	74, 190, 303, 471, 528, 649, 652
FspBI	CTAG	1 cut(s)	516
GlaI	GCGC	3 cut(s)	121, 158, 306
GluI	GCNGC	7 cut(s)	74, 190, 303, 471, 528, 649, 652
GsaI	CCCAGC	1 cut(s)	255
HaeII	RGCGCY	2 cut(s)	123, 160
HaeIII	GGCC	6 cut(s)	95, 111, 165, 332, 357, 587
HapII	CCGG	8 cut(s)	325, 343, 353, 358, 534, 593, 643, 668
HgaI	GACGC	1 cut(s)	244
HhaI	GCGC	3 cut(s)	122, 159, 307
Hin1II	CATG	1 cut(s)	553
Hin6I	GCGC	3 cut(s)	120, 157, 305
HinP1I	GCGC	3 cut(s)	120, 157, 305
HincII	GTYRAC	2 cut(s)	570, 579
HindII	GTYRAC	2 cut(s)	570, 579
HinfI	GANTC	4 cut(s)	50, 65, 102, 580
HpaII	CCGG	8 cut(s)	325, 343, 353, 358, 534, 593, 643, 668
HphI	GGTGA	3 cut(s)	133, 493, 632
Hpy166II	GTNNAC	2 cut(s)	570, 579
Hpy188I	TCNGA	4 cut(s)	49, 64, 625, 742
Hpy188III	TCNNGA	2 cut(s)	376, 557
Hpy8I	GTNNAC	2 cut(s)	570, 579
Hpy99I	CGWCG	3 cut(s)	155, 622, 625
HpyAV	CCTTC	1 cut(s)	399
HpyCH4III	ACNGT	1 cut(s)	266
HpyCH4IV	ACGT	1 cut(s)	723
HpyCH4V	TGCA	4 cut(s)	8, 222, 237, 470
HpyF10VI	GCNNNNNNNGC	5 cut(s)	14, 117, 219, 338, 527
HpyF3I	CTNAG	3 cut(s)	61, 227, 245
HpySE526I	ACGT	1 cut(s)	723
Hsp92II	CATG	1 cut(s)	553
HspAI	GCGC	3 cut(s)	120, 157, 305
Kzo9I	GATC	3 cut(s)	130, 439, 553
LguI	GCTCTTC	1 cut(s)	34
LmnI	GCTCC	3 cut(s)	117, 354, 645
Lsp1109I	GCAGC	5 cut(s)	85, 482, 514, 660, 663
MaeI	CTAG	1 cut(s)	516
MaeII	ACGT	1 cut(s)	723
MalI	GATC	3 cut(s)	132, 441, 555
MboI	GATC	3 cut(s)	130, 439, 553
MboII	GAAGA	6 cut(s)	21, 24, 45, 48, 296, 412
MflI	RGATCY	1 cut(s)	553
MhlI	GDGCHC	3 cut(s)	29, 113, 731
MlsI	TGGCCA	1 cut(s)	587
MluCI	AATT	2 cut(s)	172, 434
MluNI	TGGCCA	1 cut(s)	587
MlyI	GAGTC	3 cut(s)	44, 74, 574
MmeI	TCCRAC	2 cut(s)	72, 603
Mox20I	TGGCCA	1 cut(s)	587
MscI	TGGCCA	1 cut(s)	587
MseI	TTAA	1 cut(s)	198
Msp20I	TGGCCA	1 cut(s)	587
MspA1I	CMGCKG	1 cut(s)	527
MspI	CCGG	8 cut(s)	325, 343, 353, 358, 534, 593, 643, 668
MspR9I	CCNGG	6 cut(s)	343, 353, 354, 535, 594, 706
MvaI	CCWGG	1 cut(s)	706
MvnI	CGCG	1 cut(s)	305
MwoI	GCNNNNNNNGC	5 cut(s)	14, 117, 219, 338, 527
NciI	CCSGG	5 cut(s)	343, 353, 354, 535, 594
NcoI	CCATGG	1 cut(s)	549
NdeII	GATC	3 cut(s)	130, 439, 553
NlaIII	CATG	1 cut(s)	553
NlaIV	GGNNCC	3 cut(s)	111, 166, 547
NmeAIII	GCCGAG	2 cut(s)	121, 148
PciSI	GCTCTTC	1 cut(s)	34
PfeI	GAWTC	1 cut(s)	102
PflMI	CCANNNNNTGG	2 cut(s)	174, 436
PinAI	ACCGGT	1 cut(s)	324
PkrI	GCNGC	7 cut(s)	75, 191, 304, 472, 529, 650, 653
PleI	GAGTC	3 cut(s)	44, 73, 574
PpsI	GAGTC	3 cut(s)	44, 73, 574
Ppu21I	YACGTR	1 cut(s)	724
Psp124BI	GAGCTC	1 cut(s)	731
Psp6I	CCWGG	1 cut(s)	704
PspFI	CCCAGC	1 cut(s)	251
PspGI	CCWGG	1 cut(s)	704
PspN4I	GGNNCC	3 cut(s)	111, 166, 547
PspOMI	GGGCCC	1 cut(s)	109
PspPI	GGNCC	7 cut(s)	109, 110, 164, 330, 355, 360, 546
PstI	CTGCAG	1 cut(s)	224
PsuI	RGATCY	1 cut(s)	553
PvuII	CAGCTG	1 cut(s)	527
Rsr2I	CGGWCCG	1 cut(s)	360
RsrII	CGGWCCG	1 cut(s)	360
SacI	GAGCTC	1 cut(s)	731
SapI	GCTCTTC	1 cut(s)	34
SaqAI	TTAA	1 cut(s)	198
SatI	GCNGC	7 cut(s)	74, 190, 303, 471, 528, 649, 652
Sau3AI	GATC	3 cut(s)	130, 439, 553
Sau96I	GGNCC	7 cut(s)	109, 110, 164, 330, 355, 360, 546
SbfI	CCTGCAGG	1 cut(s)	224
SchI	GAGTC	3 cut(s)	44, 74, 574
ScrFI	CCNGG	6 cut(s)	343, 353, 354, 535, 594, 706
SdaI	CCTGCAGG	1 cut(s)	224
SduI	GDGCHC	3 cut(s)	29, 113, 731
SetI	ASST	7 cut(s)	47, 233, 246, 483, 529, 726, 731
SfcI	CTRYAG	1 cut(s)	220
SinI	GGWCC	2 cut(s)	360, 546
SmaI	CCCGGG	1 cut(s)	354
SpeI	ACTAGT	1 cut(s)	515
Sse8387I	CCTGCAGG	1 cut(s)	224
Sse9I	AATT	2 cut(s)	172, 434
SsiI	CCGC	6 cut(s)	145, 190, 303, 313, 371, 671
SspI	AATATT	2 cut(s)	13, 460
SspMI	CTAG	1 cut(s)	516
SstI	GAGCTC	1 cut(s)	731
StyD4I	CCNGG	6 cut(s)	341, 351, 352, 533, 592, 704
StyI	CCWWGG	1 cut(s)	549
TaaI	ACNGT	1 cut(s)	266
TaiI	ACGT	1 cut(s)	726
TaqI	TCGA	2 cut(s)	153, 392
TaqII	GACCGA	2 cut(s)	377, 587
TasI	AATT	2 cut(s)	172, 434
TauI	GCSGC	2 cut(s)	192, 305
TfiI	GAWTC	1 cut(s)	102
Tru1I	TTAA	1 cut(s)	198
Tru9I	TTAA	1 cut(s)	198
TscAI	CASTG	1 cut(s)	389
TseI	GCWGC	5 cut(s)	73, 470, 527, 648, 651
TspDTI	ATGAA	1 cut(s)	746
TspMI	CCCGGG	1 cut(s)	352
TspRI	CASTG	1 cut(s)	389
Van91I	CCANNNNNTGG	2 cut(s)	174, 436
VpaK11BI	GGWCC	2 cut(s)	360, 546
XmaI	CCCGGG	1 cut(s)	352
XspI	CTAG	1 cut(s)	516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.