Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0016275

Physical Location & Seq

Genomic Coordinates Reverse (-)

63566577 .. 63568301

Gene Structure 1725 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG468000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 768 bp

ATGGATGCAAATATTGCCATGAATATGTGCTCTTCTTCTACTTCTACCTCCGACTCTTCTTCCTCGGAGTCCTCGCAACCCAGAAATCCCAACAAGGCCGAGAGAATCAAGGGCCCATGGAGCGCCGAGGAGGACCGGGTTCTCACCCGCCTCGTCGAGCGCTACGGCCCCAGAAATTGGTCTTTGATTAGCCGCTACATTAAGGGTCGCTCGGGCAAGTCCTGCAGGCTTAGGTGGTGCAACCAGCTCAGCCCCAGCGTCCAGCACCGTCCCTTTTCCCAAGCCGAAGACGAAACCATCCTCGCCGCGCACGCCCGCTACGGCAACCGGTGGGCCACCATTGCCCGGTTGCTCCCGGGCCGGACCGACAATGCGGTCAAGAACCACTGGAACTCGACCCTGAAGAGAAGGGTAAGAGAACCACAAGACCACCAAGAACTGGATCATCAGGGCGAGGGTAATATTGGCATTGCAGCGATTGAGGTGATGATGATGGGTAATACCACTACTACTACTATTACTAGTGCTTCAGCTGCCTCCGGGCGTAATGGGTCCATGGATCTGGATGACCCGTTGACCGAGTTGACTCTGGCCACGCCGGGGATTGGTGGCTCTGTGGTGTCGTCGTCGGAACAACACCACCATCACCAGAGCAGCAGCGAGAGTTTTCCTCCGACGGGGTTTTGGGATGTGATGAGGGGTGTGATTGCCAGGGAAGTTAGGGATTACGTGAGCTCGTCATTTTCAGAAACTTCTGGGCTTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009751 GO:0009753 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0046686 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000022 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

255

Amino Acids

28.15

Weight (kDa)

7.17

Isoelectric Point (pI)

53.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	37 - 83	6.3e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	40 - 99	8.8e-18	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	90 - 133	1e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	92 - 146	6e-10	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016275)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35010
malus_domestica	MD08G1107400.v1.1 MD15G1088000.v1.1
prunus_persica	Prupe.1G441700_v2.0.a1
pyrus_communis	pycom08g08930 pycom15g08260
rosa_chinensis	RchiOBHm_Chr6g0307001
rosa_laevigata	RLG00000010748
rosa_roxburghii	Rroxscaffold_7G00161290
rosa_rugosa	Rorug06G0354900
rosa_samantha	Rh6AG466700 Rh6BG434300 Rh6CG481700 Rh6DG468000
rosa_wichuraiana	Rw6G040660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	374
AccB7I	CCANNNNNTGG	2 cut(s)	177, 439
AccII	CGCG	1 cut(s)	308
AciI	CCGC	5 cut(s)	148, 193, 306, 316, 374
AclWI	GGATC	2 cut(s)	450, 567
AcoI	YGGCCR	1 cut(s)	591
AcuI	CTGAAG	2 cut(s)	422, 513
AfeI	AGCGCT	1 cut(s)	161
AfiI	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 600, 605, 677
AgeI	ACCGGT	1 cut(s)	327
AhlI	ACTAGT	1 cut(s)	521
AjnI	CCWGG	1 cut(s)	710
AluBI	AGCT	3 cut(s)	247, 533, 735
AluI	AGCT	3 cut(s)	247, 533, 735
Alw21I	GWGCWC	2 cut(s)	32, 737
AlwI	GGATC	2 cut(s)	450, 567
Ama87I	CYCGRG	2 cut(s)	211, 355
Aor51HI	AGCGCT	1 cut(s)	161
AoxI	GGCC	6 cut(s)	96, 112, 166, 333, 358, 591
ApaI	GGGCCC	1 cut(s)	116
ApeKI	GCWGC	4 cut(s)	473, 533, 654, 657
AsiGI	ACCGGT	1 cut(s)	327
AspLEI	GCGC	3 cut(s)	125, 162, 310
AspS9I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 552
AsuC2I	CCSGG	6 cut(s)	137, 346, 356, 357, 541, 600
AsuHPI	GGTGA	3 cut(s)	136, 496, 638
AvaI	CYCGRG	2 cut(s)	211, 355
AvaII	GGWCC	3 cut(s)	133, 363, 552
BaeGI	GKGCMC	1 cut(s)	116
BalI	TGGCCA	1 cut(s)	593
BanII	GRGCYC	2 cut(s)	116, 737
BbsI	GAAGAC	1 cut(s)	294
Bbv12I	GWGCWC	2 cut(s)	32, 737
BbvI	GCAGC	4 cut(s)	485, 520, 666, 669
BccI	CCATC	3 cut(s)	305, 487, 651
BceAI	ACGGC	2 cut(s)	181, 337
BciT130I	CCWGG	1 cut(s)	712
BcnI	CCSGG	6 cut(s)	137, 346, 356, 357, 541, 600
BcuI	ACTAGT	1 cut(s)	521
BfaI	CTAG	1 cut(s)	522
BfmI	CTRYAG	1 cut(s)	223
BfoI	RGCGCY	2 cut(s)	126, 163
BisI	GCNGC	6 cut(s)	193, 306, 474, 534, 655, 658
BlpI	GCTNAGC	1 cut(s)	248
BlsI	GCNGC	6 cut(s)	194, 307, 475, 535, 656, 659
Bme1390I	CCNGG	7 cut(s)	137, 346, 356, 357, 541, 600, 712
Bme18I	GGWCC	3 cut(s)	133, 363, 552
BmeT110I	CYCGRG	2 cut(s)	211, 355
BmgT120I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 552
BmiI	GGNNCC	3 cut(s)	114, 169, 553
BmrFI	CCNGG	7 cut(s)	137, 346, 356, 357, 541, 600, 712
BpiI	GAAGAC	1 cut(s)	294
BplI	GAGNNNNNCTC	2 cut(s)	655, 687
Bpu10I	CCTNAGC	1 cut(s)	230
Bpu1102I	GCTNAGC	1 cut(s)	248
BpuMI	CCSGG	6 cut(s)	137, 346, 356, 357, 541, 600
BsaAI	YACGTR	1 cut(s)	730
BsaJI	CCNNGG	7 cut(s)	63, 116, 126, 355, 555, 599, 711
BsaWI	WCCGGW	1 cut(s)	327
Bsc4I	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 600, 605, 677
Bse118I	RCCGGY	1 cut(s)	327
Bse1I	ACTGG	2 cut(s)	392, 444
Bse3DI	GCAATG	2 cut(s)	339, 468
BseBI	CCWGG	1 cut(s)	712
BseDI	CCNNGG	7 cut(s)	63, 116, 126, 355, 555, 599, 711
BseGI	GGATG	4 cut(s)	10, 297, 571, 694
BseLI	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 600, 605, 677
BseMI	GCAATG	2 cut(s)	339, 468
BseMII	CTCAG	1 cut(s)	262
BseNI	ACTGG	2 cut(s)	392, 444
BseRI	GAGGAG	1 cut(s)	143
BseSI	GKGCMC	1 cut(s)	116
BseXI	GCAGC	4 cut(s)	485, 520, 666, 669
BseYI	CCCAGC	1 cut(s)	254
Bsh1236I	CGCG	1 cut(s)	308
BshFI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 593
BshTI	ACCGGT	1 cut(s)	327
BsiHKAI	GWGCWC	2 cut(s)	32, 737
BsiHKCI	CYCGRG	2 cut(s)	211, 355
BsiSI	CCGG	7 cut(s)	136, 328, 346, 356, 361, 540, 599
BslFI	GGGAC	1 cut(s)	255
BslI	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 600, 605, 677
BsmFI	GGGAC	1 cut(s)	255
BsnI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 593
BsoBI	CYCGRG	2 cut(s)	211, 355
Bsp120I	GGGCCC	1 cut(s)	112
Bsp1286I	GDGCHC	3 cut(s)	32, 116, 737
Bsp143I	GATC	2 cut(s)	442, 559
Bsp1720I	GCTNAGC	1 cut(s)	248
Bsp19I	CCATGG	2 cut(s)	116, 555
BspACI	CCGC	5 cut(s)	148, 193, 306, 316, 374
BspANI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 593
BspCNI	CTCAG	1 cut(s)	261
BspFNI	CGCG	1 cut(s)	308
BspLI	GGNNCC	3 cut(s)	114, 169, 553
BspMAI	CTGCAG	1 cut(s)	227
BspPI	GGATC	2 cut(s)	450, 567
BspQI	GCTCTTC	1 cut(s)	37
BsrDI	GCAATG	2 cut(s)	339, 468
BsrFI	RCCGGY	1 cut(s)	327
BsrI	ACTGG	2 cut(s)	392, 444
BssAI	RCCGGY	1 cut(s)	327
BssECI	CCNNGG	7 cut(s)	63, 116, 126, 355, 555, 599, 711
BssMI	GATC	2 cut(s)	442, 559
BssT1I	CCWWGG	2 cut(s)	116, 555
Bst2UI	CCWGG	1 cut(s)	712
Bst4CI	ACNGT	1 cut(s)	269
Bst6I	CTCTTC	3 cut(s)	37, 61, 398
BstAPI	GCANNNNNTGC	2 cut(s)	14, 222
BstBAI	YACGTR	1 cut(s)	730
BstC8I	GCNNGC	3 cut(s)	227, 312, 316
BstDEI	CTNAG	2 cut(s)	230, 248
BstDSI	CCRYGG	2 cut(s)	116, 555
BstF5I	GGATG	4 cut(s)	10, 297, 571, 694
BstFNI	CGCG	1 cut(s)	308
BstH2I	RGCGCY	2 cut(s)	126, 163
BstHHI	GCGC	3 cut(s)	125, 162, 310
BstKTI	GATC	2 cut(s)	445, 562
BstMBI	GATC	2 cut(s)	442, 559
BstMWI	GCNNNNNNNGC	6 cut(s)	14, 120, 222, 311, 341, 533
BstNI	CCWGG	1 cut(s)	712
BstSCI	CCNGG	7 cut(s)	135, 344, 354, 355, 539, 598, 710
BstSFI	CTRYAG	1 cut(s)	223
BstSLI	GKGCMC	1 cut(s)	116
BstUI	CGCG	1 cut(s)	308
BstV1I	GCAGC	4 cut(s)	485, 520, 666, 669
BstV2I	GAAGAC	1 cut(s)	294
BstX2I	RGATCY	1 cut(s)	559
BstXI	CCANNNNNNTGG	1 cut(s)	562
BstYI	RGATCY	1 cut(s)	559
BsuRI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 593
BtgI	CCRYGG	2 cut(s)	116, 555
BtsCI	GGATG	4 cut(s)	10, 297, 571, 694
BtsIMutI	CAGTG	1 cut(s)	385
Cac8I	GCNNGC	3 cut(s)	227, 312, 316
CfoI	GCGC	3 cut(s)	125, 162, 310
Cfr10I	RCCGGY	1 cut(s)	327
Cfr13I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 552
Cfr9I	CCCGGG	1 cut(s)	355
CpoI	CGGWCCG	1 cut(s)	363
CseI	GACGC	1 cut(s)	247
CspAI	ACCGGT	1 cut(s)	327
CspI	CGGWCCG	1 cut(s)	363
CviAII	CATG	3 cut(s)	19, 117, 556
DdeI	CTNAG	2 cut(s)	230, 248
DpnI	GATC	2 cut(s)	444, 561
DpnII	GATC	2 cut(s)	442, 559
DrdI	GACNNNNNNGTC	1 cut(s)	374
DseDI	GACNNNNNNGTC	1 cut(s)	374
EaeI	YGGCCR	1 cut(s)	591
Eam1104I	CTCTTC	3 cut(s)	37, 61, 398
EarI	CTCTTC	3 cut(s)	37, 61, 398
Ecl136II	GAGCTC	1 cut(s)	735
Eco130I	CCWWGG	2 cut(s)	116, 555
Eco24I	GRGCYC	2 cut(s)	116, 737
Eco47I	GGWCC	3 cut(s)	133, 363, 552
Eco47III	AGCGCT	1 cut(s)	161
Eco53kI	GAGCTC	1 cut(s)	735
Eco57I	CTGAAG	2 cut(s)	422, 513
Eco88I	CYCGRG	2 cut(s)	211, 355
EcoICRI	GAGCTC	1 cut(s)	735
EcoO109I	RGGNCCY	1 cut(s)	112
EcoRII	CCWGG	1 cut(s)	710
EcoT14I	CCWWGG	2 cut(s)	116, 555
EcoT38I	GRGCYC	2 cut(s)	116, 737
ErhI	CCWWGG	2 cut(s)	116, 555
FaeI	CATG	3 cut(s)	22, 120, 559
FaiI	YATR	4 cut(s)	20, 26, 118, 557
FaqI	GGGAC	1 cut(s)	255
FatI	CATG	3 cut(s)	18, 116, 555
FauI	CCCGC	2 cut(s)	155, 323
Fnu4HI	GCNGC	6 cut(s)	193, 306, 474, 534, 655, 658
FokI	GGATG	4 cut(s)	17, 284, 578, 701
FriOI	GRGCYC	2 cut(s)	116, 737
Fsp4HI	GCNGC	6 cut(s)	193, 306, 474, 534, 655, 658
FspBI	CTAG	1 cut(s)	522
GlaI	GCGC	3 cut(s)	124, 161, 309
GluI	GCNGC	6 cut(s)	193, 306, 474, 534, 655, 658
GsaI	CCCAGC	1 cut(s)	258
HaeII	RGCGCY	2 cut(s)	126, 163
HaeIII	GGCC	6 cut(s)	98, 114, 168, 335, 360, 593
HapII	CCGG	7 cut(s)	136, 328, 346, 356, 361, 540, 599
HgaI	GACGC	1 cut(s)	247
HhaI	GCGC	3 cut(s)	125, 162, 310
Hin1II	CATG	3 cut(s)	22, 120, 559
Hin6I	GCGC	3 cut(s)	123, 160, 308
HinP1I	GCGC	3 cut(s)	123, 160, 308
HincII	GTYRAC	2 cut(s)	576, 585
HindII	GTYRAC	2 cut(s)	576, 585
HinfI	GANTC	4 cut(s)	53, 68, 105, 586
HpaII	CCGG	7 cut(s)	136, 328, 346, 356, 361, 540, 599
HphI	GGTGA	3 cut(s)	136, 496, 638
Hpy166II	GTNNAC	2 cut(s)	576, 585
Hpy188I	TCNGA	5 cut(s)	52, 67, 631, 675, 748
Hpy188III	TCNNGA	2 cut(s)	379, 563
Hpy8I	GTNNAC	2 cut(s)	576, 585
Hpy99I	CGWCG	4 cut(s)	158, 628, 631, 679
HpyAV	CCTTC	1 cut(s)	402
HpyCH4III	ACNGT	1 cut(s)	269
HpyCH4IV	ACGT	1 cut(s)	729
HpyCH4V	TGCA	4 cut(s)	8, 225, 240, 473
HpyF10VI	GCNNNNNNNGC	6 cut(s)	14, 120, 222, 311, 341, 533
HpyF3I	CTNAG	2 cut(s)	230, 248
HpySE526I	ACGT	1 cut(s)	729
Hsp92II	CATG	3 cut(s)	22, 120, 559
HspAI	GCGC	3 cut(s)	123, 160, 308
Kzo9I	GATC	2 cut(s)	442, 559
LguI	GCTCTTC	1 cut(s)	37
LmnI	GCTCC	2 cut(s)	120, 357
Lsp1109I	GCAGC	4 cut(s)	485, 520, 666, 669
MaeI	CTAG	1 cut(s)	522
MaeII	ACGT	1 cut(s)	729
MalI	GATC	2 cut(s)	444, 561
MboI	GATC	2 cut(s)	442, 559
MboII	GAAGA	6 cut(s)	24, 27, 48, 51, 299, 415
MflI	RGATCY	1 cut(s)	559
MhlI	GDGCHC	3 cut(s)	32, 116, 737
MlsI	TGGCCA	1 cut(s)	593
MluCI	AATT	1 cut(s)	175
MluNI	TGGCCA	1 cut(s)	593
MlyI	GAGTC	3 cut(s)	47, 77, 580
MmeI	TCCRAC	3 cut(s)	75, 609, 698
Mox20I	TGGCCA	1 cut(s)	593
MscI	TGGCCA	1 cut(s)	593
MseI	TTAA	1 cut(s)	201
MslI	CAYNNNNRTG	1 cut(s)	23
Msp20I	TGGCCA	1 cut(s)	593
MspA1I	CMGCKG	1 cut(s)	533
MspI	CCGG	7 cut(s)	136, 328, 346, 356, 361, 540, 599
MspR9I	CCNGG	7 cut(s)	137, 346, 356, 357, 541, 600, 712
MvaI	CCWGG	1 cut(s)	712
MvnI	CGCG	1 cut(s)	308
MwoI	GCNNNNNNNGC	6 cut(s)	14, 120, 222, 311, 341, 533
NciI	CCSGG	6 cut(s)	137, 346, 356, 357, 541, 600
NcoI	CCATGG	2 cut(s)	116, 555
NdeII	GATC	2 cut(s)	442, 559
NlaIII	CATG	3 cut(s)	22, 120, 559
NlaIV	GGNNCC	3 cut(s)	114, 169, 553
NmeAIII	GCCGAG	2 cut(s)	124, 151
PciSI	GCTCTTC	1 cut(s)	37
PfeI	GAWTC	1 cut(s)	105
PflMI	CCANNNNNTGG	2 cut(s)	177, 439
PinAI	ACCGGT	1 cut(s)	327
PkrI	GCNGC	6 cut(s)	194, 307, 475, 535, 656, 659
PleI	GAGTC	3 cut(s)	47, 76, 580
PpsI	GAGTC	3 cut(s)	47, 76, 580
Ppu21I	YACGTR	1 cut(s)	730
Psp124BI	GAGCTC	1 cut(s)	737
Psp6I	CCWGG	1 cut(s)	710
PspFI	CCCAGC	1 cut(s)	254
PspGI	CCWGG	1 cut(s)	710
PspN4I	GGNNCC	3 cut(s)	114, 169, 553
PspOMI	GGGCCC	1 cut(s)	112
PspPI	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 552
PstI	CTGCAG	1 cut(s)	227
PsuI	RGATCY	1 cut(s)	559
PvuII	CAGCTG	1 cut(s)	533
RseI	CAYNNNNRTG	1 cut(s)	23
Rsr2I	CGGWCCG	1 cut(s)	363
RsrII	CGGWCCG	1 cut(s)	363
SacI	GAGCTC	1 cut(s)	737
SapI	GCTCTTC	1 cut(s)	37
SaqAI	TTAA	1 cut(s)	201
SatI	GCNGC	6 cut(s)	193, 306, 474, 534, 655, 658
Sau3AI	GATC	2 cut(s)	442, 559
Sau96I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 552
SbfI	CCTGCAGG	1 cut(s)	227
SchI	GAGTC	3 cut(s)	47, 77, 580
ScrFI	CCNGG	7 cut(s)	137, 346, 356, 357, 541, 600, 712
SdaI	CCTGCAGG	1 cut(s)	227
SduI	GDGCHC	3 cut(s)	32, 116, 737
SetI	ASST	7 cut(s)	50, 236, 249, 486, 535, 732, 737
SfcI	CTRYAG	1 cut(s)	223
SinI	GGWCC	3 cut(s)	133, 363, 552
SmaI	CCCGGG	1 cut(s)	357
SmiMI	CAYNNNNRTG	1 cut(s)	23
SpeI	ACTAGT	1 cut(s)	521
Sse8387I	CCTGCAGG	1 cut(s)	227
Sse9I	AATT	1 cut(s)	175
SsiI	CCGC	5 cut(s)	148, 193, 306, 316, 374
SspI	AATATT	2 cut(s)	13, 463
SspMI	CTAG	1 cut(s)	522
SstI	GAGCTC	1 cut(s)	737
StyD4I	CCNGG	7 cut(s)	135, 344, 354, 355, 539, 598, 710
StyI	CCWWGG	2 cut(s)	116, 555
TaaI	ACNGT	1 cut(s)	269
TaiI	ACGT	1 cut(s)	732
TaqI	TCGA	2 cut(s)	156, 395
TaqII	GACCGA	2 cut(s)	380, 593
TasI	AATT	1 cut(s)	175
TauI	GCSGC	2 cut(s)	195, 308
TfiI	GAWTC	1 cut(s)	105
Tru1I	TTAA	1 cut(s)	201
Tru9I	TTAA	1 cut(s)	201
TscAI	CASTG	1 cut(s)	392
TseI	GCWGC	4 cut(s)	473, 533, 654, 657
TspDTI	ATGAA	2 cut(s)	35, 752
TspMI	CCCGGG	1 cut(s)	355
TspRI	CASTG	1 cut(s)	392
Van91I	CCANNNNNTGG	2 cut(s)	177, 439
VpaK11BI	GGWCC	3 cut(s)	133, 363, 552
XmaI	CCCGGG	1 cut(s)	355
XspI	CTAG	1 cut(s)	522

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.