Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0016275

Physical Location & Seq

Genomic Coordinates Reverse (-)

64904791 .. 64905549

Gene Structure 759 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG466700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 759 bp

ATGGATGCAAATATTGCCATGAATATGTGCTCTTCTTCTACTTCTACCTCCGACTCTTCTTCCTCGGAGTCCTCGCAGCCCAGAAATCCCAACAAGGCCTACAGAATCAAGGGCCCGTGGAGCGCCGAGGAGGACCGGGTTCTCACCCGCCTCGTCGAGCGCTACGGCCCCAGAAATTGGTCTTTGATTAGCCGCTACATTAAGGGTCGCTCGGGCAAGTCCTGCAGGCTTAGGTGGTGCAACCAGCTCAGCCCCAGTGTCCAGCACCGTCCCTTTTCCCAAGCCGAAGACGAAACCATCCTCGCCGCGCACGCCCGCTACGGCAACCGGTGGGCCACCATTGCCCGGTTGCTCCCGGGCCGGACCGACAATGCGGTCAAGAACCACTGGAACTCGACCCTGAAGAGAAGGGTAAGAGAACCACAAGACCACCAAGAACTGGATCATCAGGGCGAGGGTAATATTGGCAATGCAGCGATTGAGGTGATGATGATGGGTAATACTACTACTAGTAGTGCTTCAGCTGCCTCCGGGCGTAATGGGTCCATGGATCTGGATGACCCGTTGACCGAGTTGACTCTGGCCACGCCGGGGATCGGTGGCTCTGTGGTGTCGTCGTCGGAACAACACCACCATCACCGGAGCAGCAGCGAGAGTTTTCCTCCGGCGGGGTTTTGGGATGTGATGAGGGGTGTGATTGCCAGGGAAGTTAGGGATTACGTCAGCTCGTCATTTTCAGAAACTTCTGGGCTTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009751 GO:0009753 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0046677 GO:0046686 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000022 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

252

Amino Acids

27.86

Weight (kDa)

8.5

Isoelectric Point (pI)

54.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	37 - 83	6.2e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	40 - 99	8.6e-18	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	90 - 133	9.9e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	92 - 146	5.9e-10	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016275)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35010
malus_domestica	MD08G1107400.v1.1 MD15G1088000.v1.1
prunus_persica	Prupe.1G441700_v2.0.a1
pyrus_communis	pycom08g08930 pycom15g08260
rosa_chinensis	RchiOBHm_Chr6g0307001
rosa_laevigata	RLG00000010748
rosa_roxburghii	Rroxscaffold_7G00161290
rosa_rugosa	Rorug06G0354900
rosa_samantha	Rh6AG466700 Rh6BG434300 Rh6CG481700 Rh6DG468000
rosa_wichuraiana	Rw6G040660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	374
AccB7I	CCANNNNNTGG	2 cut(s)	177, 439
AccII	CGCG	1 cut(s)	308
AciI	CCGC	6 cut(s)	148, 193, 306, 316, 374, 668
AclWI	GGATC	3 cut(s)	450, 558, 602
AcoI	YGGCCR	1 cut(s)	582
AcuI	CTGAAG	2 cut(s)	422, 504
AfeI	AGCGCT	1 cut(s)	161
AfiI	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 591, 596, 668
AgeI	ACCGGT	1 cut(s)	327
AhlI	ACTAGT	1 cut(s)	509
AjnI	CCWGG	1 cut(s)	701
AluBI	AGCT	3 cut(s)	247, 524, 726
AluI	AGCT	3 cut(s)	247, 524, 726
Alw21I	GWGCWC	1 cut(s)	32
AlwI	GGATC	3 cut(s)	450, 558, 602
Ama87I	CYCGRG	2 cut(s)	211, 355
Aor51HI	AGCGCT	1 cut(s)	161
AoxI	GGCC	6 cut(s)	96, 112, 166, 333, 358, 582
ApaI	GGGCCC	1 cut(s)	116
ApeKI	GCWGC	5 cut(s)	76, 473, 524, 645, 648
AsiGI	ACCGGT	1 cut(s)	327
AspLEI	GCGC	3 cut(s)	125, 162, 310
AspS9I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 543
AsuC2I	CCSGG	6 cut(s)	137, 346, 356, 357, 532, 591
AsuHPI	GGTGA	3 cut(s)	136, 496, 629
AvaI	CYCGRG	2 cut(s)	211, 355
AvaII	GGWCC	3 cut(s)	133, 363, 543
BaeGI	GKGCMC	1 cut(s)	116
BalI	TGGCCA	1 cut(s)	584
BanII	GRGCYC	1 cut(s)	116
BbsI	GAAGAC	1 cut(s)	294
Bbv12I	GWGCWC	1 cut(s)	32
BbvI	GCAGC	5 cut(s)	88, 485, 511, 657, 660
BccI	CCATC	3 cut(s)	305, 487, 642
BceAI	ACGGC	2 cut(s)	181, 337
BciT130I	CCWGG	1 cut(s)	703
BcnI	CCSGG	6 cut(s)	137, 346, 356, 357, 532, 591
BcuI	ACTAGT	1 cut(s)	509
BfaI	CTAG	1 cut(s)	510
BfmI	CTRYAG	2 cut(s)	100, 223
BfoI	RGCGCY	2 cut(s)	126, 163
BisI	GCNGC	7 cut(s)	77, 193, 306, 474, 525, 646, 649
BlpI	GCTNAGC	1 cut(s)	248
BlsI	GCNGC	7 cut(s)	78, 194, 307, 475, 526, 647, 650
Bme1390I	CCNGG	7 cut(s)	137, 346, 356, 357, 532, 591, 703
Bme18I	GGWCC	3 cut(s)	133, 363, 543
BmeT110I	CYCGRG	2 cut(s)	211, 355
BmgT120I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 543
BmiI	GGNNCC	3 cut(s)	114, 169, 544
BmrFI	CCNGG	7 cut(s)	137, 346, 356, 357, 532, 591, 703
BmrI	ACTGGG	1 cut(s)	249
BmuI	ACTGGG	1 cut(s)	249
BpiI	GAAGAC	1 cut(s)	294
BplI	GAGNNNNNCTC	2 cut(s)	646, 678
Bpu10I	CCTNAGC	1 cut(s)	230
Bpu1102I	GCTNAGC	1 cut(s)	248
BpuMI	CCSGG	6 cut(s)	137, 346, 356, 357, 532, 591
BsaJI	CCNNGG	7 cut(s)	63, 116, 126, 355, 546, 590, 702
BsaWI	WCCGGW	2 cut(s)	327, 639
Bsc4I	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 591, 596, 668
Bse118I	RCCGGY	1 cut(s)	327
Bse1I	ACTGG	3 cut(s)	255, 392, 444
Bse3DI	GCAATG	2 cut(s)	339, 475
BseBI	CCWGG	1 cut(s)	703
BseDI	CCNNGG	7 cut(s)	63, 116, 126, 355, 546, 590, 702
BseGI	GGATG	4 cut(s)	10, 297, 562, 685
BseLI	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 591, 596, 668
BseMI	GCAATG	2 cut(s)	339, 475
BseMII	CTCAG	1 cut(s)	262
BseNI	ACTGG	3 cut(s)	255, 392, 444
BseRI	GAGGAG	1 cut(s)	143
BseSI	GKGCMC	1 cut(s)	116
BseXI	GCAGC	5 cut(s)	88, 485, 511, 657, 660
Bsh1236I	CGCG	1 cut(s)	308
BshFI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 584
BshTI	ACCGGT	1 cut(s)	327
BsiHKAI	GWGCWC	1 cut(s)	32
BsiHKCI	CYCGRG	2 cut(s)	211, 355
BsiSI	CCGG	9 cut(s)	136, 328, 346, 356, 361, 531, 590, 640, 665
BslFI	GGGAC	1 cut(s)	255
BslI	CCNNNNNNNGG	6 cut(s)	177, 345, 439, 591, 596, 668
BsmFI	GGGAC	1 cut(s)	255
BsnI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 584
BsoBI	CYCGRG	2 cut(s)	211, 355
Bsp120I	GGGCCC	1 cut(s)	112
Bsp1286I	GDGCHC	2 cut(s)	32, 116
Bsp143I	GATC	3 cut(s)	442, 550, 594
Bsp1720I	GCTNAGC	1 cut(s)	248
Bsp19I	CCATGG	1 cut(s)	546
BspACI	CCGC	6 cut(s)	148, 193, 306, 316, 374, 668
BspANI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 584
BspCNI	CTCAG	1 cut(s)	261
BspFNI	CGCG	1 cut(s)	308
BspLI	GGNNCC	3 cut(s)	114, 169, 544
BspMAI	CTGCAG	1 cut(s)	227
BspPI	GGATC	3 cut(s)	450, 558, 602
BspQI	GCTCTTC	1 cut(s)	37
BsrDI	GCAATG	2 cut(s)	339, 475
BsrFI	RCCGGY	1 cut(s)	327
BsrI	ACTGG	3 cut(s)	255, 392, 444
BssAI	RCCGGY	1 cut(s)	327
BssECI	CCNNGG	7 cut(s)	63, 116, 126, 355, 546, 590, 702
BssMI	GATC	3 cut(s)	442, 550, 594
BssT1I	CCWWGG	1 cut(s)	546
Bst2UI	CCWGG	1 cut(s)	703
Bst4CI	ACNGT	1 cut(s)	269
Bst6I	CTCTTC	3 cut(s)	37, 61, 398
BstAPI	GCANNNNNTGC	2 cut(s)	14, 222
BstC8I	GCNNGC	3 cut(s)	227, 312, 316
BstDEI	CTNAG	2 cut(s)	230, 248
BstDSI	CCRYGG	2 cut(s)	116, 546
BstF5I	GGATG	4 cut(s)	10, 297, 562, 685
BstFNI	CGCG	1 cut(s)	308
BstH2I	RGCGCY	2 cut(s)	126, 163
BstHHI	GCGC	3 cut(s)	125, 162, 310
BstKTI	GATC	3 cut(s)	445, 553, 597
BstMBI	GATC	3 cut(s)	442, 550, 594
BstMWI	GCNNNNNNNGC	6 cut(s)	14, 120, 222, 311, 341, 524
BstNI	CCWGG	1 cut(s)	703
BstSCI	CCNGG	7 cut(s)	135, 344, 354, 355, 530, 589, 701
BstSFI	CTRYAG	2 cut(s)	100, 223
BstSLI	GKGCMC	1 cut(s)	116
BstUI	CGCG	1 cut(s)	308
BstV1I	GCAGC	5 cut(s)	88, 485, 511, 657, 660
BstV2I	GAAGAC	1 cut(s)	294
BstX2I	RGATCY	1 cut(s)	550
BstXI	CCANNNNNNTGG	1 cut(s)	553
BstYI	RGATCY	1 cut(s)	550
BsuRI	GGCC	6 cut(s)	98, 114, 168, 335, 360, 584
BtgI	CCRYGG	2 cut(s)	116, 546
BtsCI	GGATG	4 cut(s)	10, 297, 562, 685
BtsIMutI	CAGTG	2 cut(s)	262, 385
Cac8I	GCNNGC	3 cut(s)	227, 312, 316
CfoI	GCGC	3 cut(s)	125, 162, 310
Cfr10I	RCCGGY	1 cut(s)	327
Cfr13I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 543
Cfr9I	CCCGGG	1 cut(s)	355
CpoI	CGGWCCG	1 cut(s)	363
CspAI	ACCGGT	1 cut(s)	327
CspI	CGGWCCG	1 cut(s)	363
CviAII	CATG	2 cut(s)	19, 547
DdeI	CTNAG	2 cut(s)	230, 248
DpnI	GATC	3 cut(s)	444, 552, 596
DpnII	GATC	3 cut(s)	442, 550, 594
DrdI	GACNNNNNNGTC	1 cut(s)	374
DseDI	GACNNNNNNGTC	1 cut(s)	374
EaeI	YGGCCR	1 cut(s)	582
Eam1104I	CTCTTC	3 cut(s)	37, 61, 398
EarI	CTCTTC	3 cut(s)	37, 61, 398
Eco130I	CCWWGG	1 cut(s)	546
Eco147I	AGGCCT	1 cut(s)	98
Eco24I	GRGCYC	1 cut(s)	116
Eco47I	GGWCC	3 cut(s)	133, 363, 543
Eco47III	AGCGCT	1 cut(s)	161
Eco57I	CTGAAG	2 cut(s)	422, 504
Eco88I	CYCGRG	2 cut(s)	211, 355
EcoO109I	RGGNCCY	1 cut(s)	112
EcoRII	CCWGG	1 cut(s)	701
EcoT14I	CCWWGG	1 cut(s)	546
EcoT38I	GRGCYC	1 cut(s)	116
ErhI	CCWWGG	1 cut(s)	546
FaeI	CATG	2 cut(s)	22, 550
FaiI	YATR	3 cut(s)	20, 26, 548
FaqI	GGGAC	1 cut(s)	255
FatI	CATG	2 cut(s)	18, 546
FauI	CCCGC	3 cut(s)	155, 323, 661
Fnu4HI	GCNGC	7 cut(s)	77, 193, 306, 474, 525, 646, 649
FokI	GGATG	4 cut(s)	17, 284, 569, 692
FriOI	GRGCYC	1 cut(s)	116
Fsp4HI	GCNGC	7 cut(s)	77, 193, 306, 474, 525, 646, 649
FspBI	CTAG	1 cut(s)	510
GlaI	GCGC	3 cut(s)	124, 161, 309
GluI	GCNGC	7 cut(s)	77, 193, 306, 474, 525, 646, 649
HaeII	RGCGCY	2 cut(s)	126, 163
HaeIII	GGCC	6 cut(s)	98, 114, 168, 335, 360, 584
HapII	CCGG	9 cut(s)	136, 328, 346, 356, 361, 531, 590, 640, 665
HhaI	GCGC	3 cut(s)	125, 162, 310
Hin1II	CATG	2 cut(s)	22, 550
Hin6I	GCGC	3 cut(s)	123, 160, 308
HinP1I	GCGC	3 cut(s)	123, 160, 308
HincII	GTYRAC	2 cut(s)	567, 576
HindII	GTYRAC	2 cut(s)	567, 576
HinfI	GANTC	4 cut(s)	53, 68, 105, 577
HpaII	CCGG	9 cut(s)	136, 328, 346, 356, 361, 531, 590, 640, 665
HphI	GGTGA	3 cut(s)	136, 496, 629
Hpy166II	GTNNAC	2 cut(s)	567, 576
Hpy188I	TCNGA	4 cut(s)	52, 67, 622, 739
Hpy188III	TCNNGA	2 cut(s)	379, 554
Hpy8I	GTNNAC	2 cut(s)	567, 576
Hpy99I	CGWCG	3 cut(s)	158, 619, 622
HpyAV	CCTTC	1 cut(s)	402
HpyCH4III	ACNGT	1 cut(s)	269
HpyCH4IV	ACGT	1 cut(s)	720
HpyCH4V	TGCA	4 cut(s)	8, 225, 240, 473
HpyF10VI	GCNNNNNNNGC	6 cut(s)	14, 120, 222, 311, 341, 524
HpyF3I	CTNAG	2 cut(s)	230, 248
HpySE526I	ACGT	1 cut(s)	720
Hsp92II	CATG	2 cut(s)	22, 550
HspAI	GCGC	3 cut(s)	123, 160, 308
Kzo9I	GATC	3 cut(s)	442, 550, 594
LguI	GCTCTTC	1 cut(s)	37
LmnI	GCTCC	3 cut(s)	120, 357, 642
Lsp1109I	GCAGC	5 cut(s)	88, 485, 511, 657, 660
MaeI	CTAG	1 cut(s)	510
MaeII	ACGT	1 cut(s)	720
MalI	GATC	3 cut(s)	444, 552, 596
MboI	GATC	3 cut(s)	442, 550, 594
MboII	GAAGA	6 cut(s)	24, 27, 48, 51, 299, 415
MflI	RGATCY	1 cut(s)	550
MhlI	GDGCHC	2 cut(s)	32, 116
MlsI	TGGCCA	1 cut(s)	584
MluCI	AATT	1 cut(s)	175
MluNI	TGGCCA	1 cut(s)	584
MlyI	GAGTC	3 cut(s)	47, 77, 571
MmeI	TCCRAC	2 cut(s)	75, 600
Mox20I	TGGCCA	1 cut(s)	584
MscI	TGGCCA	1 cut(s)	584
MseI	TTAA	1 cut(s)	201
MslI	CAYNNNNRTG	1 cut(s)	23
Msp20I	TGGCCA	1 cut(s)	584
MspA1I	CMGCKG	1 cut(s)	524
MspI	CCGG	9 cut(s)	136, 328, 346, 356, 361, 531, 590, 640, 665
MspR9I	CCNGG	7 cut(s)	137, 346, 356, 357, 532, 591, 703
MvaI	CCWGG	1 cut(s)	703
MvnI	CGCG	1 cut(s)	308
MwoI	GCNNNNNNNGC	6 cut(s)	14, 120, 222, 311, 341, 524
NciI	CCSGG	6 cut(s)	137, 346, 356, 357, 532, 591
NcoI	CCATGG	1 cut(s)	546
NdeII	GATC	3 cut(s)	442, 550, 594
NlaIII	CATG	2 cut(s)	22, 550
NlaIV	GGNNCC	3 cut(s)	114, 169, 544
NmeAIII	GCCGAG	1 cut(s)	151
PceI	AGGCCT	1 cut(s)	98
PciSI	GCTCTTC	1 cut(s)	37
PfeI	GAWTC	1 cut(s)	105
PflMI	CCANNNNNTGG	2 cut(s)	177, 439
PinAI	ACCGGT	1 cut(s)	327
PkrI	GCNGC	7 cut(s)	78, 194, 307, 475, 526, 647, 650
PleI	GAGTC	3 cut(s)	47, 76, 571
PpsI	GAGTC	3 cut(s)	47, 76, 571
Psp6I	CCWGG	1 cut(s)	701
PspGI	CCWGG	1 cut(s)	701
PspN4I	GGNNCC	3 cut(s)	114, 169, 544
PspOMI	GGGCCC	1 cut(s)	112
PspPI	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 543
PstI	CTGCAG	1 cut(s)	227
PsuI	RGATCY	1 cut(s)	550
PvuII	CAGCTG	1 cut(s)	524
RseI	CAYNNNNRTG	1 cut(s)	23
Rsr2I	CGGWCCG	1 cut(s)	363
RsrII	CGGWCCG	1 cut(s)	363
SapI	GCTCTTC	1 cut(s)	37
SaqAI	TTAA	1 cut(s)	201
SatI	GCNGC	7 cut(s)	77, 193, 306, 474, 525, 646, 649
Sau3AI	GATC	3 cut(s)	442, 550, 594
Sau96I	GGNCC	8 cut(s)	112, 113, 133, 167, 333, 358, 363, 543
SbfI	CCTGCAGG	1 cut(s)	227
SchI	GAGTC	3 cut(s)	47, 77, 571
ScrFI	CCNGG	7 cut(s)	137, 346, 356, 357, 532, 591, 703
SdaI	CCTGCAGG	1 cut(s)	227
SduI	GDGCHC	2 cut(s)	32, 116
SetI	ASST	7 cut(s)	50, 236, 249, 486, 526, 723, 728
SfcI	CTRYAG	2 cut(s)	100, 223
SinI	GGWCC	3 cut(s)	133, 363, 543
SmaI	CCCGGG	1 cut(s)	357
SmiMI	CAYNNNNRTG	1 cut(s)	23
SpeI	ACTAGT	1 cut(s)	509
Sse8387I	CCTGCAGG	1 cut(s)	227
Sse9I	AATT	1 cut(s)	175
SseBI	AGGCCT	1 cut(s)	98
SsiI	CCGC	6 cut(s)	148, 193, 306, 316, 374, 668
SspI	AATATT	2 cut(s)	13, 463
SspMI	CTAG	1 cut(s)	510
StuI	AGGCCT	1 cut(s)	98
StyD4I	CCNGG	7 cut(s)	135, 344, 354, 355, 530, 589, 701
StyI	CCWWGG	1 cut(s)	546
TaaI	ACNGT	1 cut(s)	269
TaiI	ACGT	1 cut(s)	723
TaqI	TCGA	2 cut(s)	156, 395
TaqII	GACCGA	2 cut(s)	380, 584
TasI	AATT	1 cut(s)	175
TauI	GCSGC	2 cut(s)	195, 308
TfiI	GAWTC	1 cut(s)	105
Tru1I	TTAA	1 cut(s)	201
Tru9I	TTAA	1 cut(s)	201
TscAI	CASTG	2 cut(s)	262, 392
TseI	GCWGC	5 cut(s)	76, 473, 524, 645, 648
TspDTI	ATGAA	2 cut(s)	35, 743
TspMI	CCCGGG	1 cut(s)	355
TspRI	CASTG	2 cut(s)	262, 392
Van91I	CCANNNNNTGG	2 cut(s)	177, 439
VpaK11BI	GGWCC	3 cut(s)	133, 363, 543
XmaI	CCCGGG	1 cut(s)	355
XspI	CTAG	1 cut(s)	510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.