RLG00000016581

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015911
Physical Location & Seq
Forward (+)
7859311 .. 7859952
642 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016581

Sequence Viewer

Length: 642 bp
ATGGTGAAGCAGACGATCGGGAAGCCATCGGAGCGAAGCGACGCCAAGTACAAAGGTGTTCGGAAGCGCAAGTGGGGCAAATGGGTCTCCGAGATCCGCCTACCCAACAGCCGCGAACGCATCTGGTTGGGCTCCTACGACACCCCCGAGAAAGCGGCGCGTGCCTTCGACGCGGCTCTGTTCTGCCTCCGCGGCCGCACCGCCAAGTTCAACTTCCCCGACAACCCTCCAGACATCTCCGGCGGCCGCTCCCTCTCCCCCGCCGAGATCCAGGCCGAGGCTGCCAGGTTCGCCAATAACGAGGCGAACCCTCCGCCGCCGCCGCAGGTCCCGAGGAGCCACTCCGATCTTTCCATGTCGGAGCAGCAGACGGAGTCCCCGTCGGTCTCGGAGGGGGAGGGCAGTGGGACTCTCGTCATGGACAGTGCTAGTGACTTTAGCGTGACGCTGAACCAAAACGACGAGTCGTTTTCGGACTTGTTTAGAACGTTCGGGTCGGGTAACTACGCGACGGAGTATGGATTGTTCCCGGGTTTTGACGATCTCAACGGTCAGTTTTTCAGTCCGGCACCTCAAACGCCGCCAGCCGTTGATTACGCCGAGGAGAATTTAGCCCAGGAGTCGTTCCTATGGAATTTCTAA

Protein Analysis

214

Amino Acids

23.57

Weight (kDa)

4.88

Isoelectric Point (pI)

58.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 16 - 65 6e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015911)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19210
fragaria_vesca FvH4_1g09180
malus_domestica MD02G1096500.v1.1 MD15G1221100.v1.1
prunus_persica Prupe.7G194400_v2.0.a1
pyrus_communis pycom02g07650 pycom15g19580
rosa_chinensis RchiOBHm_Chr2g0095581
rosa_laevigata RLG00000016581
rosa_multiflora Rmu_sc0004719.1_g000008
rosa_rugosa Rorug02G0052500
rosa_samantha Rh2BG101800 Rh2CG104000 Rh2DG101400
rosa_wichuraiana Rw2G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 249
AccII CGCG 5 cut(s) 114, 160, 173, 192, 509
AclI AACGTT 1 cut(s) 488
AclWI GGATC 2 cut(s) 88, 262
AcoI YGGCCR 2 cut(s) 193, 244
AcsI RAATTY 2 cut(s) 607, 634
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 3 cut(s) 270, 284, 615
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
Alw26I GTCTC 2 cut(s) 91, 391
AlwI GGATC 2 cut(s) 88, 262
Ama87I CYCGRG 3 cut(s) 146, 331, 529
AoxI GGCC 3 cut(s) 193, 244, 273
ApeKI GCWGC 2 cut(s) 281, 364
ApoI RAATTY 2 cut(s) 607, 634
AspLEI GCGC 2 cut(s) 69, 160
AspS9I GGNCC 1 cut(s) 328
AsuC2I CCSGG 2 cut(s) 530, 531
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 3 cut(s) 146, 331, 529
AvaII GGWCC 1 cut(s) 328
BanI GGYRCC 1 cut(s) 568
BanII GRGCYC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 268, 376
BccI CCATC 1 cut(s) 34
BceAI ACGGC 1 cut(s) 572
BciT130I CCWGG 3 cut(s) 272, 286, 617
BcnI CCSGG 2 cut(s) 530, 531
BcoDI GTCTC 2 cut(s) 91, 391
BfaI CTAG 1 cut(s) 429
BfuAI ACCTGC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 192
Bme1390I CCNGG 5 cut(s) 272, 286, 530, 531, 617
Bme18I GGWCC 1 cut(s) 328
BmeT110I CYCGRG 3 cut(s) 146, 331, 529
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 4 cut(s) 133, 330, 338, 570
BmrFI CCNGG 5 cut(s) 272, 286, 530, 531, 617
BmsI GCATC 1 cut(s) 129
BoxI GACNNNNGTC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 213
BpuMI CCSGG 2 cut(s) 530, 531
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 2 cut(s) 91, 391
BsaJI CCNNGG 6 cut(s) 190, 276, 332, 529, 600, 615
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 1 cut(s) 277
BseBI CCWGG 3 cut(s) 272, 286, 617
BseDI CCNNGG 6 cut(s) 190, 276, 332, 529, 600, 615
BseLI CCNNNNNNNGG 1 cut(s) 277
BseRI GAGGAG 2 cut(s) 349, 617
BseX3I CGGCCG 2 cut(s) 193, 244
BseXI GCAGC 2 cut(s) 268, 376
Bsh1236I CGCG 5 cut(s) 114, 160, 173, 192, 509
Bsh1285I CGRYCG 3 cut(s) 18, 196, 247
BshFI GGCC 3 cut(s) 195, 246, 275
BshNI GGYRCC 1 cut(s) 568
BsiEI CGRYCG 3 cut(s) 18, 196, 247
BsiHKCI CYCGRG 3 cut(s) 146, 331, 529
BsiSI CCGG 3 cut(s) 240, 530, 566
BslFI GGGAC 3 cut(s) 314, 361, 421
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 2 cut(s) 91, 391
BsmFI GGGAC 3 cut(s) 314, 361, 421
BsnI GGCC 3 cut(s) 195, 246, 275
Bso31I GGTCTC 2 cut(s) 91, 391
BsoBI CYCGRG 3 cut(s) 146, 331, 529
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 5 cut(s) 15, 93, 267, 346, 541
BspANI GGCC 3 cut(s) 195, 246, 275
BspFNI CGCG 5 cut(s) 114, 160, 173, 192, 509
BspLI GGNNCC 4 cut(s) 133, 330, 338, 570
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 2 cut(s) 88, 262
BspT107I GGYRCC 1 cut(s) 568
BspTNI GGTCTC 2 cut(s) 91, 391
BsrBI CCGCTC 1 cut(s) 249
BssECI CCNNGG 6 cut(s) 190, 276, 332, 529, 600, 615
BssMI GATC 5 cut(s) 15, 93, 267, 346, 541
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 3 cut(s) 272, 286, 617
Bst4CI ACNGT 2 cut(s) 425, 551
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 162, 585
BstDSI CCRYGG 1 cut(s) 190
BstFNI CGCG 5 cut(s) 114, 160, 173, 192, 509
BstHHI GCGC 2 cut(s) 69, 160
BstKTI GATC 5 cut(s) 18, 96, 270, 349, 544
BstMAI GTCTC 2 cut(s) 91, 391
BstMBI GATC 5 cut(s) 15, 93, 267, 346, 541
BstMCI CGRYCG 3 cut(s) 18, 196, 247
BstMWI GCNNNNNNNGC 9 cut(s) 31, 75, 117, 161, 170, 192, 281, 290, 322
BstNI CCWGG 3 cut(s) 272, 286, 617
BstPAI GACNNNNGTC 1 cut(s) 413
BstSCI CCNGG 5 cut(s) 270, 284, 528, 529, 615
BstUI CGCG 5 cut(s) 114, 160, 173, 192, 509
BstV1I GCAGC 2 cut(s) 268, 376
BstX2I RGATCY 2 cut(s) 93, 267
BstYI RGATCY 2 cut(s) 93, 267
BstZI CGGCCG 2 cut(s) 193, 244
BsuRI GGCC 3 cut(s) 195, 246, 275
BtgI CCRYGG 1 cut(s) 190
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 409, 430
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 2 cut(s) 162, 585
CciNI GCGGCCGC 2 cut(s) 193, 244
CfoI GCGC 2 cut(s) 69, 160
Cfr13I GGNCC 1 cut(s) 328
Cfr42I CCGCGG 1 cut(s) 193
Cfr9I CCCGGG 1 cut(s) 529
CseI GACGC 3 cut(s) 50, 179, 454
Csp6I GTAC 1 cut(s) 49
CviAII CATG 2 cut(s) 355, 418
CviQI GTAC 1 cut(s) 49
DpnI GATC 5 cut(s) 17, 95, 269, 348, 543
DpnII GATC 5 cut(s) 15, 93, 267, 346, 541
EaeI YGGCCR 2 cut(s) 193, 244
EagI CGGCCG 2 cut(s) 193, 244
EciI GGCGGA 2 cut(s) 86, 303
EclXI CGGCCG 2 cut(s) 193, 244
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 2 cut(s) 91, 391
Eco47I GGWCC 1 cut(s) 328
Eco52I CGGCCG 2 cut(s) 193, 244
Eco88I CYCGRG 3 cut(s) 146, 331, 529
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 3 cut(s) 270, 284, 615
EcoT38I GRGCYC 1 cut(s) 134
FaeI CATG 2 cut(s) 358, 421
FaiI YATR 4 cut(s) 356, 419, 519, 631
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 314, 361, 421
FatI CATG 2 cut(s) 354, 417
FauI CCCGC 1 cut(s) 268
FriOI GRGCYC 1 cut(s) 134
FspBI CTAG 1 cut(s) 429
GlaI GCGC 2 cut(s) 68, 159
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 3 cut(s) 195, 246, 275
HapII CCGG 3 cut(s) 240, 530, 566
HgaI GACGC 3 cut(s) 50, 179, 454
HhaI GCGC 2 cut(s) 69, 160
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 2 cut(s) 358, 421
Hin6I GCGC 2 cut(s) 67, 158
HinP1I GCGC 2 cut(s) 67, 158
HinfI GANTC 4 cut(s) 374, 409, 464, 620
HpaII CCGG 3 cut(s) 240, 530, 566
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 7 cut(s) 31, 63, 91, 346, 361, 391, 475
Hpy188III TCNNGA 3 cut(s) 19, 230, 331
Hpy99I CGWCG 5 cut(s) 44, 173, 385, 464, 514
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 425, 551
HpyCH4IV ACGT 1 cut(s) 488
HpyF10VI GCNNNNNNNGC 9 cut(s) 31, 75, 117, 161, 170, 192, 281, 290, 322
HpySE526I ACGT 1 cut(s) 488
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 2 cut(s) 358, 421
HspAI GCGC 2 cut(s) 67, 158
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 5 cut(s) 15, 93, 267, 346, 541
LmnI GCTCC 5 cut(s) 31, 137, 254, 336, 361
Lsp1109I GCAGC 2 cut(s) 268, 376
LweI GCATC 1 cut(s) 129
MaeI CTAG 1 cut(s) 429
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 3 cut(s) 431, 442, 500
MalI GATC 5 cut(s) 17, 95, 269, 348, 543
MbiI CCGCTC 1 cut(s) 249
MboI GATC 5 cut(s) 15, 93, 267, 346, 541
MflI RGATCY 2 cut(s) 93, 267
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 2 cut(s) 607, 634
MlyI GAGTC 4 cut(s) 383, 403, 473, 629
MmeI TCCRAC 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 3 cut(s) 240, 530, 566
MspR9I CCNGG 5 cut(s) 272, 286, 530, 531, 617
MvaI CCWGG 3 cut(s) 272, 286, 617
MvnI CGCG 5 cut(s) 114, 160, 173, 192, 509
MwoI GCNNNNNNNGC 9 cut(s) 31, 75, 117, 161, 170, 192, 281, 290, 322
NciI CCSGG 2 cut(s) 530, 531
NdeII GATC 5 cut(s) 15, 93, 267, 346, 541
NlaIII CATG 2 cut(s) 358, 421
NlaIV GGNNCC 4 cut(s) 133, 330, 338, 570
NmeAIII GCCGAG 3 cut(s) 289, 301, 625
NmuCI GTSAC 2 cut(s) 431, 442
NotI GCGGCCGC 2 cut(s) 193, 244
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 297, 377
PflFI GACNNNGTC 1 cut(s) 373
Ple19I CGATCG 1 cut(s) 18
PleI GAGTC 4 cut(s) 382, 403, 472, 628
PpsI GAGTC 4 cut(s) 382, 403, 472, 628
PpuMI RGGWCCY 1 cut(s) 328
PshAI GACNNNNGTC 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 3 cut(s) 270, 284, 615
PspGI CCWGG 3 cut(s) 270, 284, 615
PspN4I GGNNCC 4 cut(s) 133, 330, 338, 570
PspPI GGNCC 1 cut(s) 328
PspPPI RGGWCCY 1 cut(s) 328
PsrI GAACNNNNNNTAC 2 cut(s) 509, 541
PsuI RGATCY 2 cut(s) 93, 267
PsyI GACNNNGTC 1 cut(s) 373
PvuI CGATCG 1 cut(s) 18
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacII CCGCGG 1 cut(s) 193
Sau3AI GATC 5 cut(s) 15, 93, 267, 346, 541
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 4 cut(s) 383, 403, 473, 629
ScrFI CCNGG 5 cut(s) 272, 286, 530, 531, 617
SduI GDGCHC 1 cut(s) 134
SetI ASST 5 cut(s) 58, 290, 330, 491, 574
SfaNI GCATC 1 cut(s) 129
Sfr303I CCGCGG 1 cut(s) 193
SgrBI CCGCGG 1 cut(s) 193
SinI GGWCC 1 cut(s) 328
SmaI CCCGGG 1 cut(s) 531
Sse9I AATT 2 cut(s) 607, 634
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 5 cut(s) 270, 284, 528, 529, 615
TaaI ACNGT 2 cut(s) 425, 551
TaiI ACGT 1 cut(s) 491
TaqI TCGA 1 cut(s) 168
TaqII GACCGA 1 cut(s) 373
TasI AATT 2 cut(s) 607, 634
TatI WGTACW 1 cut(s) 48
TscAI CASTG 2 cut(s) 409, 430
TseFI GTSAC 2 cut(s) 431, 442
TseI GCWGC 2 cut(s) 281, 364
Tsp45I GTSAC 2 cut(s) 431, 442
TspGWI ACGGA 2 cut(s) 386, 527
TspMI CCCGGG 1 cut(s) 529
TspRI CASTG 2 cut(s) 409, 430
Tth111I GACNNNGTC 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 328
XapI RAATTY 2 cut(s) 607, 634
XmaI CCCGGG 1 cut(s) 529
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.