Rh2CG104000

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015911
Physical Location & Seq
Forward (+)
9004363 .. 9005874
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG104000.1

Sequence Viewer

Length: 633 bp
ATGGTGAAGCAGACGATCGGGAAGCCATCGGAGCGAAGCGACGCCAAGTACAAAGGTGTTCGGAAGCGCAAGTGGGGCAAATGGGTCTCCGAGATCCGCCTACCCAACAGCCGCGAACGCATCTGGTTGGGCTCCTACGACACCCCCGAGAAAGCGGCGCGTGCCTTCGACGCGGCTCTGTTCTGCCTCCGCGGCCGCACCGCCAAGTTCAACTTCCCCGACAACCCTCCAGACATCTCCGGCGGCCGCTCCCTCTCCCCCGCCGAGATCCAGGCCGAGGCCGCCAGGTTCGCCAATAACGAGGAGAACCCTCCGCCGCCGCAGGTCCCCAGGAGCCACTCTGATCTTTCCATGTCGGAGCAGCAGACGGAGTCCCCGTCTGTCTCGGAGGGGAGTGGGACCCTCGTCATGGACAGTGCCGCTAGTGACTTTGGCGTGACGCTGAACCAAAACGACGAGTCGTTTTCGGACTTGTTTAGAACGTTCGGGTCGGGTAACTACGCGACGGAGTATGGATTGTTCCCGGGTTTTGACGATCTCAACGGTCAGTTTTTCAGTCCGGCACCTCAAACGCCAGCCGTTGATTACGCCGAGGAGAATTTAGCTCAGGAGTCGTTCCTATGGAATTTCTAA

Protein Analysis

210

Amino Acids

23.29

Weight (kDa)

4.88

Isoelectric Point (pI)

59.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 16 - 65 5.8e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015911)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19210
fragaria_vesca FvH4_1g09180
malus_domestica MD02G1096500.v1.1 MD15G1221100.v1.1
prunus_persica Prupe.7G194400_v2.0.a1
pyrus_communis pycom02g07650 pycom15g19580
rosa_chinensis RchiOBHm_Chr2g0095581
rosa_laevigata RLG00000016581
rosa_multiflora Rmu_sc0004719.1_g000008
rosa_rugosa Rorug02G0052500
rosa_samantha Rh2BG101800 Rh2CG104000 Rh2DG101400
rosa_wichuraiana Rw2G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 1 cut(s) 249
AccII CGCG 5 cut(s) 114, 160, 173, 192, 503
AclI AACGTT 1 cut(s) 482
AclWI GGATC 2 cut(s) 88, 262
AcoI YGGCCR 2 cut(s) 193, 244
AcsI RAATTY 2 cut(s) 598, 625
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 277, 409
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 3 cut(s) 270, 284, 329
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 1 cut(s) 605
AluI AGCT 1 cut(s) 605
Alw26I GTCTC 2 cut(s) 91, 388
AlwI GGATC 2 cut(s) 88, 262
Ama87I CYCGRG 2 cut(s) 146, 523
AoxI GGCC 4 cut(s) 193, 244, 273, 279
ApeKI GCWGC 1 cut(s) 361
ApoI RAATTY 2 cut(s) 598, 625
AspLEI GCGC 2 cut(s) 69, 160
AspS9I GGNCC 2 cut(s) 325, 399
AsuC2I CCSGG 2 cut(s) 524, 525
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 2 cut(s) 146, 523
AvaII GGWCC 2 cut(s) 325, 399
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 34
BceAI ACGGC 1 cut(s) 563
BciT130I CCWGG 3 cut(s) 272, 286, 331
BcnI CCSGG 2 cut(s) 524, 525
BcoDI GTCTC 2 cut(s) 91, 388
BfaI CTAG 1 cut(s) 423
BfuAI ACCTGC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 192
Bme1390I CCNGG 5 cut(s) 272, 286, 331, 524, 525
Bme18I GGWCC 2 cut(s) 325, 399
BmeT110I CYCGRG 2 cut(s) 146, 523
BmgT120I GGNCC 2 cut(s) 325, 399
BmiI GGNNCC 6 cut(s) 133, 327, 335, 400, 401, 564
BmrFI CCNGG 5 cut(s) 272, 286, 331, 524, 525
BmsI GCATC 1 cut(s) 129
BoxI GACNNNNGTC 1 cut(s) 404
BpmI CTGGAG 1 cut(s) 213
Bpu10I CCTNAGC 1 cut(s) 606
BpuMI CCSGG 2 cut(s) 524, 525
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 5 cut(s) 190, 276, 329, 523, 591
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 409
BseBI CCWGG 3 cut(s) 272, 286, 331
BseDI CCNNGG 5 cut(s) 190, 276, 329, 523, 591
BseLI CCNNNNNNNGG 2 cut(s) 277, 409
BseMII CTCAG 1 cut(s) 620
BseRI GAGGAG 2 cut(s) 317, 608
BseX3I CGGCCG 2 cut(s) 193, 244
BseXI GCAGC 1 cut(s) 373
Bsh1236I CGCG 5 cut(s) 114, 160, 173, 192, 503
Bsh1285I CGRYCG 3 cut(s) 18, 196, 247
BshFI GGCC 4 cut(s) 195, 246, 275, 281
BshNI GGYRCC 1 cut(s) 562
BsiEI CGRYCG 3 cut(s) 18, 196, 247
BsiHKCI CYCGRG 2 cut(s) 146, 523
BsiSI CCGG 3 cut(s) 240, 524, 560
BslFI GGGAC 3 cut(s) 311, 358, 412
BslI CCNNNNNNNGG 2 cut(s) 277, 409
BsmAI GTCTC 2 cut(s) 91, 388
BsmFI GGGAC 3 cut(s) 311, 358, 412
BsnI GGCC 4 cut(s) 195, 246, 275, 281
Bso31I GGTCTC 1 cut(s) 91
BsoBI CYCGRG 2 cut(s) 146, 523
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 5 cut(s) 15, 93, 267, 343, 535
BspANI GGCC 4 cut(s) 195, 246, 275, 281
BspCNI CTCAG 1 cut(s) 619
BspFNI CGCG 5 cut(s) 114, 160, 173, 192, 503
BspLI GGNNCC 6 cut(s) 133, 327, 335, 400, 401, 564
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 2 cut(s) 88, 262
BspT107I GGYRCC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 249
BssECI CCNNGG 5 cut(s) 190, 276, 329, 523, 591
BssMI GATC 5 cut(s) 15, 93, 267, 343, 535
BssNI GRCGYC 1 cut(s) 42
Bst2UI CCWGG 3 cut(s) 272, 286, 331
Bst4CI ACNGT 2 cut(s) 416, 545
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 162, 576
BstDEI CTNAG 1 cut(s) 606
BstDSI CCRYGG 1 cut(s) 190
BstFNI CGCG 5 cut(s) 114, 160, 173, 192, 503
BstHHI GCGC 2 cut(s) 69, 160
BstKTI GATC 5 cut(s) 18, 96, 270, 346, 538
BstMAI GTCTC 2 cut(s) 91, 388
BstMBI GATC 5 cut(s) 15, 93, 267, 343, 535
BstMCI CGRYCG 3 cut(s) 18, 196, 247
BstMWI GCNNNNNNNGC 8 cut(s) 31, 75, 117, 161, 170, 192, 281, 290
BstNI CCWGG 3 cut(s) 272, 286, 331
BstPAI GACNNNNGTC 1 cut(s) 404
BstSCI CCNGG 5 cut(s) 270, 284, 329, 522, 523
BstUI CGCG 5 cut(s) 114, 160, 173, 192, 503
BstV1I GCAGC 1 cut(s) 373
BstX2I RGATCY 2 cut(s) 93, 267
BstYI RGATCY 2 cut(s) 93, 267
BstZI CGGCCG 2 cut(s) 193, 244
BsuRI GGCC 4 cut(s) 195, 246, 275, 281
BtgI CCRYGG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 421
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 162, 576
CciNI GCGGCCGC 2 cut(s) 193, 244
CfoI GCGC 2 cut(s) 69, 160
Cfr13I GGNCC 2 cut(s) 325, 399
Cfr42I CCGCGG 1 cut(s) 193
Cfr9I CCCGGG 1 cut(s) 523
CseI GACGC 3 cut(s) 50, 179, 448
Csp6I GTAC 1 cut(s) 49
CviAII CATG 2 cut(s) 352, 409
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 1 cut(s) 606
DpnI GATC 5 cut(s) 17, 95, 269, 345, 537
DpnII GATC 5 cut(s) 15, 93, 267, 343, 535
EaeI YGGCCR 2 cut(s) 193, 244
EagI CGGCCG 2 cut(s) 193, 244
EciI GGCGGA 2 cut(s) 86, 303
EclXI CGGCCG 2 cut(s) 193, 244
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 91
Eco47I GGWCC 2 cut(s) 325, 399
Eco52I CGGCCG 2 cut(s) 193, 244
Eco88I CYCGRG 2 cut(s) 146, 523
EcoO109I RGGNCCY 2 cut(s) 325, 399
EcoRII CCWGG 3 cut(s) 270, 284, 329
EcoT38I GRGCYC 1 cut(s) 134
FaeI CATG 2 cut(s) 355, 412
FaiI YATR 4 cut(s) 353, 410, 513, 622
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 311, 358, 412
FatI CATG 2 cut(s) 351, 408
FauI CCCGC 1 cut(s) 268
FriOI GRGCYC 1 cut(s) 134
FspBI CTAG 1 cut(s) 423
GlaI GCGC 2 cut(s) 68, 159
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 4 cut(s) 195, 246, 275, 281
HapII CCGG 3 cut(s) 240, 524, 560
HgaI GACGC 3 cut(s) 50, 179, 448
HhaI GCGC 2 cut(s) 69, 160
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 2 cut(s) 355, 412
Hin6I GCGC 2 cut(s) 67, 158
HinP1I GCGC 2 cut(s) 67, 158
HinfI GANTC 3 cut(s) 371, 458, 611
HpaII CCGG 3 cut(s) 240, 524, 560
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 7 cut(s) 31, 63, 91, 343, 358, 388, 469
Hpy188III TCNNGA 3 cut(s) 19, 230, 608
Hpy99I CGWCG 4 cut(s) 44, 173, 458, 508
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 416, 545
HpyCH4IV ACGT 1 cut(s) 482
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 75, 117, 161, 170, 192, 281, 290
HpyF3I CTNAG 1 cut(s) 606
HpySE526I ACGT 1 cut(s) 482
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 2 cut(s) 355, 412
HspAI GCGC 2 cut(s) 67, 158
KflI GGGWCCC 1 cut(s) 399
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 5 cut(s) 15, 93, 267, 343, 535
LmnI GCTCC 5 cut(s) 31, 137, 254, 333, 358
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 1 cut(s) 129
MaeI CTAG 1 cut(s) 423
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 3 cut(s) 425, 436, 494
MalI GATC 5 cut(s) 17, 95, 269, 345, 537
MbiI CCGCTC 1 cut(s) 249
MboI GATC 5 cut(s) 15, 93, 267, 343, 535
MflI RGATCY 2 cut(s) 93, 267
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 2 cut(s) 598, 625
MlyI GAGTC 3 cut(s) 380, 467, 620
MmeI TCCRAC 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 3 cut(s) 240, 524, 560
MspR9I CCNGG 5 cut(s) 272, 286, 331, 524, 525
MvaI CCWGG 3 cut(s) 272, 286, 331
MvnI CGCG 5 cut(s) 114, 160, 173, 192, 503
MwoI GCNNNNNNNGC 8 cut(s) 31, 75, 117, 161, 170, 192, 281, 290
NciI CCSGG 2 cut(s) 524, 525
NdeII GATC 5 cut(s) 15, 93, 267, 343, 535
NlaIII CATG 2 cut(s) 355, 412
NlaIV GGNNCC 6 cut(s) 133, 327, 335, 400, 401, 564
NmeAIII GCCGAG 3 cut(s) 289, 301, 616
NmuCI GTSAC 2 cut(s) 425, 436
NotI GCGGCCGC 2 cut(s) 193, 244
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 297, 374
PflFI GACNNNGTC 1 cut(s) 370
Ple19I CGATCG 1 cut(s) 18
PleI GAGTC 3 cut(s) 379, 466, 619
PpsI GAGTC 3 cut(s) 379, 466, 619
PpuMI RGGWCCY 2 cut(s) 325, 399
PshAI GACNNNNGTC 1 cut(s) 404
Psp1406I AACGTT 1 cut(s) 482
Psp5II RGGWCCY 2 cut(s) 325, 399
Psp6I CCWGG 3 cut(s) 270, 284, 329
PspGI CCWGG 3 cut(s) 270, 284, 329
PspN4I GGNNCC 6 cut(s) 133, 327, 335, 400, 401, 564
PspPI GGNCC 2 cut(s) 325, 399
PspPPI RGGWCCY 2 cut(s) 325, 399
PsrI GAACNNNNNNTAC 2 cut(s) 503, 535
PsuI RGATCY 2 cut(s) 93, 267
PsyI GACNNNGTC 1 cut(s) 370
PvuI CGATCG 1 cut(s) 18
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacII CCGCGG 1 cut(s) 193
Sau3AI GATC 5 cut(s) 15, 93, 267, 343, 535
Sau96I GGNCC 2 cut(s) 325, 399
SchI GAGTC 3 cut(s) 380, 467, 620
ScrFI CCNGG 5 cut(s) 272, 286, 331, 524, 525
SduI GDGCHC 1 cut(s) 134
SetI ASST 6 cut(s) 58, 290, 327, 485, 568, 607
SfaNI GCATC 1 cut(s) 129
Sfr303I CCGCGG 1 cut(s) 193
SgrBI CCGCGG 1 cut(s) 193
SinI GGWCC 2 cut(s) 325, 399
SmaI CCCGGG 1 cut(s) 525
Sse9I AATT 2 cut(s) 598, 625
SspMI CTAG 1 cut(s) 423
StyD4I CCNGG 5 cut(s) 270, 284, 329, 522, 523
TaaI ACNGT 2 cut(s) 416, 545
TaiI ACGT 1 cut(s) 485
TaqI TCGA 1 cut(s) 168
TasI AATT 2 cut(s) 598, 625
TatI WGTACW 1 cut(s) 48
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 2 cut(s) 425, 436
TseI GCWGC 1 cut(s) 361
Tsp45I GTSAC 2 cut(s) 425, 436
TspGWI ACGGA 2 cut(s) 383, 521
TspMI CCCGGG 1 cut(s) 523
TspRI CASTG 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 370
VpaK11BI GGWCC 2 cut(s) 325, 399
XapI RAATTY 2 cut(s) 598, 625
XmaI CCCGGG 1 cut(s) 523
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.