RLG00000018222

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008831
Physical Location & Seq
Reverse (-)
27088784 .. 27093632
4849 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018222

Sequence Viewer

Length: 720 bp
ATGAATGAACGCAGCCTCCGCCGCTGTTTTCTAGAGGTTTCCGACCCTCAAACCCTCAGATCTCACTTCCTGGTGTCTAGTTGCCGCAAAATGGAAGCTAACTACGGTTCGGTCGGAGTTAACGGCGCGTCGCTAACACGCGCCGTGGAGGCCTTGGCTGCTTTGCTGCTCTCGGCTCTGCTCAGGACCCAGCTCTTGGCTCTGGATCCCCTTGCATTTTTACCTAACAACGGAGCTTGTATTAAAGAAGATCCTCACACTACATCCCAAGCTAAGCCAGCTGATGCTGAGGGAGATGATGAAGATGATGATGGTGATGGTGGACTTGAAGAAGGTGAAGAGGACTTGTCATCTGAAGAAGGAGAAGAGTATGGCAACAAACCGAACAACAATAAGAGTAACTCAAAGAAGGCTCCAGAGGGTGGAGCAGGAGGTGCACCTTTTAGGCCACACTATCCCGAGACTTGTAAAACTACCTTGAATCTGTTGGATCCTTGCTTTCCGCTATATGATCCTTCGGAGCCTACACCGGCTTGCTGTAAAGCTGCGGCGTCTCTCAGCACTCAAGTAATTGCGAAGAAGCTATATGAGTGTCTAGAGGATTTACAAAAGGCAGGGCCTCTGAACTTATCTGCACTTCGTCTTCTGTTCCCCGCCTGTGACGTTTCTGTAAAGCTTCCACCGGCACGTGTCTCGGATGATATGGACTGTTCCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

25.6

Weight (kDa)

4.61

Isoelectric Point (pI)

57.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 196, 422
AccII CGCG 2 cut(s) 128, 141
AciI CCGC 6 cut(s) 19, 22, 85, 503, 548, 654
AclWI GGATC 6 cut(s) 200, 213, 245, 485, 498, 506
AcuI CTGAAG 1 cut(s) 375
AcvI CACGTG 1 cut(s) 689
AcyI GRCGYC 1 cut(s) 551
AfiI CCNNNNNNNGG 4 cut(s) 91, 196, 230, 422
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 2 cut(s) 329, 481
AjnI CCWGG 1 cut(s) 69
AloI GAACNNNNNNTCC 1 cut(s) 32
AluBI AGCT 8 cut(s) 98, 193, 236, 272, 281, 545, 583, 676
AluI AGCT 8 cut(s) 98, 193, 236, 272, 281, 545, 583, 676
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 3 cut(s) 455, 558, 697
Alw44I GTGCAC 1 cut(s) 435
AlwI GGATC 6 cut(s) 200, 213, 245, 485, 498, 506
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 3 cut(s) 150, 446, 617
ApaLI GTGCAC 1 cut(s) 435
ApeKI GCWGC 4 cut(s) 12, 158, 166, 545
AspLEI GCGC 2 cut(s) 128, 143
AspS9I GGNCC 2 cut(s) 186, 617
AsuHPI GGTGA 2 cut(s) 326, 347
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 1 cut(s) 186
BaeGI GKGCMC 1 cut(s) 439
BamHI GGATCC 2 cut(s) 205, 490
BbrPI CACGTG 1 cut(s) 689
BbsI GAAGAC 1 cut(s) 635
Bbv12I GWGCWC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 24, 145, 153, 532
BccI CCATC 2 cut(s) 305, 311
BceAI ACGGC 2 cut(s) 128, 139
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BciT130I CCWGG 1 cut(s) 71
BcoDI GTCTC 3 cut(s) 455, 558, 697
BfaI CTAG 3 cut(s) 32, 78, 596
BglI GCCNNNNNGGC 1 cut(s) 149
BglII AGATCT 1 cut(s) 59
BisI GCNGC 7 cut(s) 13, 22, 85, 159, 167, 546, 549
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 7 cut(s) 14, 23, 86, 160, 168, 547, 550
Bme1390I CCNGG 1 cut(s) 71
Bme18I GGWCC 1 cut(s) 186
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 2 cut(s) 186, 617
BmiI GGNNCC 5 cut(s) 188, 207, 414, 492, 522
BmrFI CCNGG 1 cut(s) 71
BmrI ACTGGG 1 cut(s) 709
BmsI GCATC 1 cut(s) 274
BmuI ACTGGG 1 cut(s) 709
BpiI GAAGAC 1 cut(s) 635
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 2 cut(s) 182, 288
Bpu1102I GCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 549
BsaAI YACGTR 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 144, 153
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 196, 230, 422
Bse118I RCCGGY 2 cut(s) 529, 682
Bse1I ACTGG 1 cut(s) 715
BseBI CCWGG 1 cut(s) 71
BseDI CCNNGG 2 cut(s) 144, 153
BseGI GGATG 2 cut(s) 263, 703
BseLI CCNNNNNNNGG 4 cut(s) 91, 196, 230, 422
BseMII CTCAG 4 cut(s) 70, 196, 279, 571
BseNI ACTGG 1 cut(s) 715
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 4 cut(s) 24, 145, 153, 532
BseYI CCCAGC 1 cut(s) 189
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 2 cut(s) 128, 141
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 152, 448, 619
BsiEI CGRYCG 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 2 cut(s) 530, 683
BslI CCNNNNNNNGG 4 cut(s) 91, 196, 230, 422
BsmAI GTCTC 3 cut(s) 455, 558, 697
BsmBI CGTCTC 1 cut(s) 558
BsnI GGCC 3 cut(s) 152, 448, 619
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 5 cut(s) 59, 205, 250, 490, 511
Bsp1720I GCTNAGC 1 cut(s) 273
BspACI CCGC 6 cut(s) 19, 22, 85, 503, 548, 654
BspANI GGCC 3 cut(s) 152, 448, 619
BspCNI CTCAG 4 cut(s) 69, 195, 280, 570
BspFNI CGCG 2 cut(s) 128, 141
BspLI GGNNCC 5 cut(s) 188, 207, 414, 492, 522
BspPI GGATC 6 cut(s) 200, 213, 245, 485, 498, 506
BsrFI RCCGGY 2 cut(s) 529, 682
BsrI ACTGG 1 cut(s) 715
BssAI RCCGGY 2 cut(s) 529, 682
BssECI CCNNGG 2 cut(s) 144, 153
BssMI GATC 5 cut(s) 59, 205, 250, 490, 511
BssNI GRCGYC 1 cut(s) 551
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 107, 710
Bst6I CTCTTC 2 cut(s) 333, 360
BstACI GRCGYC 1 cut(s) 551
BstAPI GCANNNNNTGC 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 279, 535
BstDEI CTNAG 5 cut(s) 56, 182, 273, 288, 557
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 2 cut(s) 263, 703
BstFNI CGCG 2 cut(s) 128, 141
BstHHI GCGC 2 cut(s) 128, 143
BstKTI GATC 5 cut(s) 62, 208, 253, 493, 514
BstMAI GTCTC 3 cut(s) 455, 558, 697
BstMBI GATC 5 cut(s) 59, 205, 250, 490, 511
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 6 cut(s) 18, 21, 149, 158, 278, 434
BstNI CCWGG 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 439
BstUI CGCG 2 cut(s) 128, 141
BstV1I GCAGC 4 cut(s) 24, 145, 153, 532
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 4 cut(s) 59, 205, 250, 490
BstYI RGATCY 4 cut(s) 59, 205, 250, 490
BsuRI GGCC 3 cut(s) 152, 448, 619
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 2 cut(s) 263, 703
Cac8I GCNNGC 2 cut(s) 279, 535
CfoI GCGC 2 cut(s) 128, 143
Cfr10I RCCGGY 2 cut(s) 529, 682
Cfr13I GGNCC 2 cut(s) 186, 617
CseI GACGC 2 cut(s) 117, 540
DdeI CTNAG 5 cut(s) 56, 182, 273, 288, 557
DpnI GATC 5 cut(s) 61, 207, 252, 492, 513
DpnII GATC 5 cut(s) 59, 205, 250, 490, 511
Eam1104I CTCTTC 2 cut(s) 333, 360
EarI CTCTTC 2 cut(s) 333, 360
EciI GGCGGA 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 375
Eco72I CACGTG 1 cut(s) 689
Eco88I CYCGRG 1 cut(s) 458
EcoO109I RGGNCCY 2 cut(s) 186, 617
EcoRII CCWGG 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 558
FaiI YATR 6 cut(s) 372, 508, 510, 586, 588, 704
FauI CCCGC 1 cut(s) 661
Fnu4HI GCNGC 7 cut(s) 13, 22, 85, 159, 167, 546, 549
FokI GGATG 2 cut(s) 250, 710
Fsp4HI GCNGC 7 cut(s) 13, 22, 85, 159, 167, 546, 549
FspBI CTAG 3 cut(s) 32, 78, 596
GlaI GCGC 2 cut(s) 127, 142
GluI GCNGC 7 cut(s) 13, 22, 85, 159, 167, 546, 549
GsaI CCCAGC 1 cut(s) 193
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 152, 448, 619
HapII CCGG 2 cut(s) 530, 683
HgaI GACGC 2 cut(s) 117, 540
HhaI GCGC 2 cut(s) 128, 143
Hin1I GRCGYC 1 cut(s) 551
Hin6I GCGC 2 cut(s) 126, 141
HinP1I GCGC 2 cut(s) 126, 141
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 1 cut(s) 481
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 2 cut(s) 530, 683
HphI GGTGA 2 cut(s) 326, 347
Hpy166II GTNNAC 3 cut(s) 121, 323, 437
Hpy188I TCNGA 7 cut(s) 43, 59, 116, 355, 520, 624, 697
Hpy188III TCNNGA 6 cut(s) 32, 184, 203, 416, 458, 596
Hpy8I GTNNAC 3 cut(s) 121, 323, 437
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 4 cut(s) 326, 353, 403, 525
HpyCH4III ACNGT 2 cut(s) 107, 710
HpyCH4IV ACGT 2 cut(s) 663, 688
HpyCH4V TGCA 3 cut(s) 215, 437, 635
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 21, 149, 158, 278, 434
HpyF3I CTNAG 5 cut(s) 56, 182, 273, 288, 557
HpySE526I ACGT 2 cut(s) 663, 688
Hsp92I GRCGYC 1 cut(s) 551
HspAI GCGC 2 cut(s) 126, 141
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 5 cut(s) 59, 205, 250, 490, 511
LmnI GCTCC 4 cut(s) 233, 418, 425, 520
Lsp1109I GCAGC 4 cut(s) 24, 145, 153, 532
LweI GCATC 1 cut(s) 274
MaeI CTAG 3 cut(s) 32, 78, 596
MaeII ACGT 2 cut(s) 663, 688
MaeIII GTNAC 2 cut(s) 398, 659
MalI GATC 5 cut(s) 61, 207, 252, 492, 513
MboI GATC 5 cut(s) 59, 205, 250, 490, 511
MboII GAAGA 8 cut(s) 260, 314, 341, 350, 368, 377, 589, 635
MflI RGATCY 4 cut(s) 59, 205, 250, 490
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 1 cut(s) 570
MmeI TCCRAC 3 cut(s) 66, 94, 468
MseI TTAA 2 cut(s) 120, 243
MspA1I CMGCKG 2 cut(s) 24, 281
MspI CCGG 2 cut(s) 530, 683
MspR9I CCNGG 1 cut(s) 71
MvaI CCWGG 1 cut(s) 71
MvnI CGCG 2 cut(s) 128, 141
MwoI GCNNNNNNNGC 6 cut(s) 18, 21, 149, 158, 278, 434
NdeII GATC 5 cut(s) 59, 205, 250, 490, 511
NlaIV GGNNCC 5 cut(s) 188, 207, 414, 492, 522
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 1 cut(s) 659
PceI AGGCCT 1 cut(s) 152
PfeI GAWTC 1 cut(s) 481
PflMI CCANNNNNTGG 2 cut(s) 196, 422
PkrI GCNGC 7 cut(s) 14, 23, 86, 160, 168, 547, 550
PmaCI CACGTG 1 cut(s) 689
PmlI CACGTG 1 cut(s) 689
Ppu21I YACGTR 1 cut(s) 689
PpuMI RGGWCCY 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 69
PspCI CACGTG 1 cut(s) 689
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 1 cut(s) 69
PspN4I GGNNCC 5 cut(s) 188, 207, 414, 492, 522
PspPI GGNCC 2 cut(s) 186, 617
PspPPI RGGWCCY 1 cut(s) 186
PsuI RGATCY 4 cut(s) 59, 205, 250, 490
PvuII CAGCTG 1 cut(s) 281
SaqAI TTAA 2 cut(s) 120, 243
SatI GCNGC 7 cut(s) 13, 22, 85, 159, 167, 546, 549
Sau3AI GATC 5 cut(s) 59, 205, 250, 490, 511
Sau96I GGNCC 2 cut(s) 186, 617
ScrFI CCNGG 1 cut(s) 71
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 1 cut(s) 274
SinI GGWCC 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
Sse9I AATT 1 cut(s) 570
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 6 cut(s) 19, 22, 85, 503, 548, 654
SspMI CTAG 3 cut(s) 32, 78, 596
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 69
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 107, 710
TaiI ACGT 2 cut(s) 666, 691
TaqII GACCGA 1 cut(s) 100
TasI AATT 1 cut(s) 570
TauI GCSGC 3 cut(s) 24, 87, 551
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 2 cut(s) 120, 243
Tru9I TTAA 2 cut(s) 120, 243
TseFI GTSAC 1 cut(s) 659
TseI GCWGC 4 cut(s) 12, 158, 166, 545
Tsp45I GTSAC 1 cut(s) 659
TspDTI ATGAA 3 cut(s) 17, 21, 315
TspGWI ACGGA 1 cut(s) 246
Van91I CCANNNNNTGG 2 cut(s) 196, 422
VneI GTGCAC 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 186
XbaI TCTAGA 2 cut(s) 31, 595
XspI CTAG 3 cut(s) 32, 78, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.