Rw2G020210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008831
Physical Location & Seq
Reverse (-)
27418000 .. 27420778
2779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020210.1

Sequence Viewer

Length: 909 bp
ATGATTAAAAGCTCCTTAAAACCCAAACTTGTCCTTACCTACTCTCCCGGGCAGCTCCCCACAGCAAATCTACCTACGAATCCCTCCCTGCCCGTCCTGCCCGAACTTGACTGGAGAATTACGGGCAGTCACACACCAGTACTCTCTCTCTCTCTCTCTGTGCTCCCTTCTTCTCTCTTTTTCTCTCTGATATTTTGCCCATCTTGTTTCTCTCTGTGTCTCTTCGATCACGATCCTACGGTCAAGAATGAACGCAGCCTCCGCCGCTGTTTTCTAGAGGTTTCCGGCCCTCAAACCCGCAGATCTCACTTCCTGGTGTCTGGTTGCCGCAAAATGGAAACTAACTACGGTTCGGTCGGAGTTAACGGCGCGTCGCTAGCACGCGCCGTGGAGGCCCTGGCTGCTTTGCTGCTCTCGGCTCTGCTCAGGACCACCCAGCTCTTGGCTCTGGATCCCCTTGCGTTTTTACCTAACAACGGAGCTGGTATTAAAGAAGATCCTCACACTACATCCCAAGCTAAGCCAGCTGATGCTGAGGGAGATGATGAAGATGATGATGGTGATGGTGGACTTGAAGAAGGTGAAGAGGACTTGTCATCTGAAGAAGGAGAAGAGTATGGCAACAAACCGAACAACAATAAGAGCAACTCAAAGAAGGCTCCAGAGGGTGGAGCAGGAGATGGGGCTGAAGAGAATGGAGAAGAGGAAGAGGGCGATGAAGAAGATGGTGATGACCAAGAAGATGACAATGAGGACGACAATGATGAAGAGGATGATGACGATGACGACGACGATGACAATGACGATGGAGGAGAAGAGGATGAGGAAGGAGTCGATGAGGAGAATGAGGAGGAGGAAGAAGATGAAGATGAGGAAGCACTTCAGCCCCCAAAGAAGAGGAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

302

Amino Acids

32.8

Weight (kDa)

4.05

Isoelectric Point (pI)

58.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 442, 668
AccII CGCG 2 cut(s) 371, 384
AciI CCGC 4 cut(s) 262, 265, 298, 328
AclWI GGATC 4 cut(s) 227, 446, 459, 491
AcuI CTGAAG 3 cut(s) 621, 708, 866
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 4 cut(s) 334, 442, 476, 668
AgsI TTSAA 1 cut(s) 575
AjnI CCWGG 2 cut(s) 312, 396
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 6 cut(s) 12, 55, 439, 482, 518, 527
AluI AGCT 6 cut(s) 12, 55, 439, 482, 518, 527
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 4 cut(s) 227, 446, 459, 491
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 2 cut(s) 286, 393
ApeKI GCWGC 4 cut(s) 52, 255, 401, 409
Asp700I GAANNNNTTC 1 cut(s) 879
AspLEI GCGC 2 cut(s) 371, 386
AspS9I GGNCC 3 cut(s) 287, 394, 429
AsuC2I CCSGG 2 cut(s) 48, 49
AsuHPI GGTGA 3 cut(s) 572, 593, 740
AsuNHI GCTAGC 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 429
BamHI GGATCC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 165
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 4 cut(s) 64, 267, 388, 396
BccI CCATC 6 cut(s) 208, 551, 557, 674, 719, 800
BceAI ACGGC 2 cut(s) 371, 382
BciT130I CCWGG 2 cut(s) 314, 398
BcnI CCSGG 2 cut(s) 48, 49
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 2 cut(s) 275, 377
BglI GCCNNNNNGGC 1 cut(s) 392
BglII AGATCT 1 cut(s) 302
BisI GCNGC 6 cut(s) 53, 256, 265, 328, 402, 410
BlpI GCTNAGC 1 cut(s) 519
BlsI GCNGC 6 cut(s) 54, 257, 266, 329, 403, 411
BmcAI AGTACT 1 cut(s) 141
Bme1390I CCNGG 4 cut(s) 48, 49, 314, 398
Bme18I GGWCC 1 cut(s) 429
BmeT110I CYCGRG 1 cut(s) 47
BmgT120I GGNCC 3 cut(s) 287, 394, 429
BmiI GGNNCC 2 cut(s) 453, 660
BmrFI CCNGG 4 cut(s) 48, 49, 314, 398
BmsI GCATC 1 cut(s) 520
BmtI GCTAGC 1 cut(s) 380
BpmI CTGGAG 2 cut(s) 133, 645
Bpu10I CCTNAGC 2 cut(s) 425, 534
Bpu1102I GCTNAGC 1 cut(s) 519
BpuMI CCSGG 2 cut(s) 48, 49
BsaBI GATNNNNATC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 47, 387, 396
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 243, 273
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 442, 476, 668
Bse1I ACTGG 2 cut(s) 116, 137
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 2 cut(s) 314, 398
BseDI CCNNGG 3 cut(s) 47, 387, 396
BseGI GGATG 3 cut(s) 509, 778, 826
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 4 cut(s) 334, 442, 476, 668
BseMII CTCAG 2 cut(s) 439, 525
BseNI ACTGG 2 cut(s) 116, 137
BseRI GAGGAG 4 cut(s) 825, 854, 863, 866
BseXI GCAGC 4 cut(s) 64, 267, 388, 396
BseYI CCCAGC 1 cut(s) 435
Bsh1236I CGCG 2 cut(s) 371, 384
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 2 cut(s) 288, 395
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 2 cut(s) 48, 285
BslI CCNNNNNNNGG 4 cut(s) 334, 442, 476, 668
BsmAI GTCTC 1 cut(s) 224
BsnI GGCC 2 cut(s) 288, 395
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 5 cut(s) 226, 232, 302, 451, 496
Bsp1720I GCTNAGC 1 cut(s) 519
BspACI CCGC 4 cut(s) 262, 265, 298, 328
BspANI GGCC 2 cut(s) 288, 395
BspCNI CTCAG 2 cut(s) 438, 526
BspFNI CGCG 2 cut(s) 371, 384
BspLI GGNNCC 2 cut(s) 453, 660
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 4 cut(s) 227, 446, 459, 491
BsrI ACTGG 2 cut(s) 116, 137
BssECI CCNNGG 3 cut(s) 47, 387, 396
BssMI GATC 5 cut(s) 226, 232, 302, 451, 496
Bst2UI CCWGG 2 cut(s) 314, 398
Bst4CI ACNGT 2 cut(s) 241, 350
Bst6I CTCTTC 9 cut(s) 227, 579, 606, 684, 696, 702, 762, 810, 890
BstC8I GCNNGC 3 cut(s) 378, 382, 525
BstDEI CTNAG 3 cut(s) 425, 519, 534
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 509, 778, 826
BstFNI CGCG 2 cut(s) 371, 384
BstHHI GCGC 2 cut(s) 371, 386
BstKTI GATC 5 cut(s) 229, 235, 305, 454, 499
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 5 cut(s) 226, 232, 302, 451, 496
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 7 cut(s) 97, 261, 264, 377, 392, 401, 524
BstNI CCWGG 2 cut(s) 314, 398
BstSCI CCNGG 4 cut(s) 46, 47, 312, 396
BstUI CGCG 2 cut(s) 371, 384
BstV1I GCAGC 4 cut(s) 64, 267, 388, 396
BstX2I RGATCY 3 cut(s) 302, 451, 496
BstYI RGATCY 3 cut(s) 302, 451, 496
BsuRI GGCC 2 cut(s) 288, 395
BtgI CCRYGG 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 3 cut(s) 509, 778, 826
Cac8I GCNNGC 3 cut(s) 378, 382, 525
CfoI GCGC 2 cut(s) 371, 386
Cfr13I GGNCC 3 cut(s) 287, 394, 429
Cfr9I CCCGGG 1 cut(s) 47
CseI GACGC 1 cut(s) 360
Csp6I GTAC 1 cut(s) 140
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 3 cut(s) 425, 519, 534
DpnI GATC 5 cut(s) 228, 234, 304, 453, 498
DpnII GATC 5 cut(s) 226, 232, 302, 451, 496
Eam1104I CTCTTC 9 cut(s) 227, 579, 606, 684, 696, 702, 762, 810, 890
EarI CTCTTC 9 cut(s) 227, 579, 606, 684, 696, 702, 762, 810, 890
EciI GGCGGA 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 429
Eco57I CTGAAG 3 cut(s) 621, 708, 866
Eco88I CYCGRG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 312, 396
FaiI YATR 1 cut(s) 618
FauI CCCGC 1 cut(s) 305
Fnu4HI GCNGC 6 cut(s) 53, 256, 265, 328, 402, 410
FokI GGATG 3 cut(s) 496, 785, 833
Fsp4HI GCNGC 6 cut(s) 53, 256, 265, 328, 402, 410
FspBI CTAG 2 cut(s) 275, 377
GlaI GCGC 2 cut(s) 370, 385
GluI GCNGC 6 cut(s) 53, 256, 265, 328, 402, 410
GsaI CCCAGC 1 cut(s) 439
GsuI CTGGAG 2 cut(s) 133, 645
HaeIII GGCC 2 cut(s) 288, 395
HapII CCGG 2 cut(s) 48, 285
HgaI GACGC 1 cut(s) 360
HhaI GCGC 2 cut(s) 371, 386
Hin6I GCGC 2 cut(s) 369, 384
HinP1I GCGC 2 cut(s) 369, 384
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 2 cut(s) 79, 831
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 2 cut(s) 48, 285
HphI GGTGA 3 cut(s) 572, 593, 740
Hpy166II GTNNAC 2 cut(s) 364, 569
Hpy188I TCNGA 3 cut(s) 189, 359, 601
Hpy188III TCNNGA 6 cut(s) 230, 244, 275, 427, 449, 662
Hpy8I GTNNAC 2 cut(s) 364, 569
Hpy99I CGWCG 3 cut(s) 376, 791, 794
HpyAV CCTTC 5 cut(s) 177, 572, 599, 649, 821
HpyCH4III ACNGT 2 cut(s) 241, 350
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 261, 264, 377, 392, 401, 524
HpyF3I CTNAG 3 cut(s) 425, 519, 534
HspAI GCGC 2 cut(s) 369, 384
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 5 cut(s) 226, 232, 302, 451, 496
LmnI GCTCC 6 cut(s) 17, 60, 168, 479, 664, 671
Lsp1109I GCAGC 4 cut(s) 64, 267, 388, 396
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 275, 377
MaeIII GTNAC 1 cut(s) 128
MalI GATC 5 cut(s) 228, 234, 304, 453, 498
MboI GATC 5 cut(s) 226, 232, 302, 451, 496
MflI RGATCY 3 cut(s) 302, 451, 496
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 1 cut(s) 117
MlyI GAGTC 1 cut(s) 840
MmeI TCCRAC 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 879
MseI TTAA 4 cut(s) 6, 17, 363, 489
MspA1I CMGCKG 2 cut(s) 267, 527
MspI CCGG 2 cut(s) 48, 285
MspR9I CCNGG 4 cut(s) 48, 49, 314, 398
MvaI CCWGG 2 cut(s) 314, 398
MvnI CGCG 2 cut(s) 371, 384
MwoI GCNNNNNNNGC 7 cut(s) 97, 261, 264, 377, 392, 401, 524
NciI CCSGG 2 cut(s) 48, 49
NdeII GATC 5 cut(s) 226, 232, 302, 451, 496
NheI GCTAGC 1 cut(s) 376
NlaIV GGNNCC 2 cut(s) 453, 660
NmeAIII GCCGAG 1 cut(s) 395
NmuCI GTSAC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 786
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 1 cut(s) 79
PflMI CCANNNNNTGG 2 cut(s) 442, 668
PkrI GCNGC 6 cut(s) 54, 257, 266, 329, 403, 411
PleI GAGTC 1 cut(s) 839
PpsI GAGTC 1 cut(s) 839
Psp6I CCWGG 2 cut(s) 312, 396
PspFI CCCAGC 1 cut(s) 435
PspGI CCWGG 2 cut(s) 312, 396
PspN4I GGNNCC 2 cut(s) 453, 660
PspPI GGNCC 3 cut(s) 287, 394, 429
PsuI RGATCY 3 cut(s) 302, 451, 496
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
SaqAI TTAA 4 cut(s) 6, 17, 363, 489
SatI GCNGC 6 cut(s) 53, 256, 265, 328, 402, 410
Sau3AI GATC 5 cut(s) 226, 232, 302, 451, 496
Sau96I GGNCC 3 cut(s) 287, 394, 429
ScaI AGTACT 1 cut(s) 141
SchI GAGTC 1 cut(s) 840
ScrFI CCNGG 4 cut(s) 48, 49, 314, 398
SduI GDGCHC 1 cut(s) 165
SfaNI GCATC 1 cut(s) 520
SinI GGWCC 1 cut(s) 429
SmaI CCCGGG 1 cut(s) 49
Sse9I AATT 1 cut(s) 117
SsiI CCGC 4 cut(s) 262, 265, 298, 328
SspMI CTAG 2 cut(s) 275, 377
StyD4I CCNGG 4 cut(s) 46, 47, 312, 396
TaaI ACNGT 2 cut(s) 241, 350
TaqI TCGA 2 cut(s) 225, 834
TaqII GACCGA 1 cut(s) 343
TasI AATT 1 cut(s) 117
TatI WGTACW 1 cut(s) 139
TauI GCSGC 2 cut(s) 267, 330
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 4 cut(s) 6, 17, 363, 489
Tru9I TTAA 4 cut(s) 6, 17, 363, 489
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 4 cut(s) 52, 255, 401, 409
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 5 cut(s) 264, 561, 732, 780, 879
TspGWI ACGGA 1 cut(s) 492
TspMI CCCGGG 1 cut(s) 47
Van91I CCANNNNNTGG 2 cut(s) 442, 668
VpaK11BI GGWCC 1 cut(s) 429
XbaI TCTAGA 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XmaI CCCGGG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 2 cut(s) 275, 377
ZrmI AGTACT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.