RLG00000021769

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014103
Physical Location & Seq
Reverse (-)
78052793 .. 78053527
735 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021769

Sequence Viewer

Length: 735 bp
ATGGATCCGGATGAACCCCAAGACTCTGGAATCAAAATCTACAACAACGCCGTGTACGGCGACCCACCCGATCCCGAAGACCAGGCCAAGCCCAAGTCCGCCTCCTCCTCCTCCTGCATCGGCTCCAACCCCAGTAAGCCACCTTCTTCATCTCTTCCTCTCGTCCGCCTCAACCACGACCAACTCCACGGCCGCAAACGACGAGCCGTCGCCAAAGGCGTCCAGAAAACCATCTCCAAAACCTCTATGCTCGTCAACTTCCTCCCCACCGGCACCCTCCTCACTTTCGAGATGGTCCTTCCCGCTATCTACCGCAACGGCGACTGCACCACCGTCACTACCACCATGACTCACGTCCTCCTAGGGATCTGCTCCCTCTCCTGCTTCTTCTTCCACTTCACCGACAGTTTTAAAGGCGCCGATGGGAAGATGTACTACGGCTTCGTGACGCCGAAGGGGTTGGCGGTTTTCAAGCCGGGGCTGACGGTGGACGTGCCTAAGGATGAGAGGTATAGGGTGGGGCTGACGGACTTTGTTCATGCCATAATGTCAGTAATGGTGTTCATGGCGATTGCGTTTTCGGACCGGCGCGTGACGGAGTGTATGTTTCCTGGGCACGAGAAGGAGATGGATGAGGTAATGGAGAGTTTCCCGTTGATGGTGGGAATTGTTTGCAGTGGCTTGTTTCTTGTGTTTCCGAATTCTCGCCATGGGATTGGCTGCATGGCTGCATGA

Protein Analysis

245

Amino Acids

26.75

Weight (kDa)

7.03

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 80 - 241 1.5e-65 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09157 AT5G39650
fragaria_vesca FvH4_6g48440
malus_domestica MD00G1130800.v1.1 MD09G1051900.v1.1
prunus_persica Prupe.3G266800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0168351
rosa_laevigata RLG00000021769
rosa_multiflora Rmu_sc0003354.1_g000017
rosa_roxburghii Rroxscaffold_2G00083120
rosa_rugosa Rorug02G0533100
rosa_samantha Rh2AG602200 Rh2BG615500 Rh2CG583200 Rh2DG625700
rosa_wichuraiana Rw0G019820 Rw2G049990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 272, 416
AccII CGCG 1 cut(s) 591
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 6 cut(s) 99, 166, 193, 303, 313, 464
AclWI GGATC 3 cut(s) 12, 65, 374
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 700
AcyI GRCGYC 3 cut(s) 219, 417, 449
AfaI GTAC 2 cut(s) 56, 434
AfiI CCNNNNNNNGG 1 cut(s) 658
AgsI TTSAA 1 cut(s) 472
AhdI GACNNNNNGTC 1 cut(s) 206
AjiI CACGTC 2 cut(s) 355, 493
AjnI CCWGG 2 cut(s) 81, 610
AlwI GGATC 3 cut(s) 12, 65, 374
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 84, 190
ApeKI GCWGC 2 cut(s) 720, 728
ApoI RAATTY 1 cut(s) 700
AspA2I CCTAGG 1 cut(s) 361
AspLEI GCGC 2 cut(s) 419, 591
AspS9I GGNCC 2 cut(s) 295, 583
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 1 cut(s) 391
AvaII GGWCC 2 cut(s) 295, 583
AvrII CCTAGG 1 cut(s) 361
AxyI CCTNAGG 1 cut(s) 498
BaeGI GKGCMC 1 cut(s) 618
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 272, 416
BarI GAAGNNNNNNTAC 6 cut(s) 127, 159, 419, 425, 451, 457
BauI CACGAG 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 707, 715
BccI CCATC 5 cut(s) 239, 286, 416, 622, 652
BceAI ACGGC 6 cut(s) 35, 73, 191, 205, 334, 454
BciT130I CCWGG 2 cut(s) 83, 612
BcnI CCSGG 1 cut(s) 477
BfaI CTAG 1 cut(s) 362
BfoI RGCGCY 1 cut(s) 420
BisI GCNGC 3 cut(s) 193, 721, 729
BlnI CCTAGG 1 cut(s) 361
BlsI GCNGC 3 cut(s) 194, 722, 730
Bme1390I CCNGG 3 cut(s) 83, 477, 612
Bme18I GGWCC 2 cut(s) 295, 583
BmeRI GACNNNNNGTC 1 cut(s) 206
BmgBI CACGTC 2 cut(s) 355, 493
BmgT120I GGNCC 2 cut(s) 295, 583
BmiI GGNNCC 4 cut(s) 6, 124, 274, 418
BmrFI CCNGG 3 cut(s) 83, 477, 612
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 126
BoxI GACNNNNGTC 1 cut(s) 353
BpiI GAAGAC 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 477
BsaHI GRCGYC 3 cut(s) 219, 417, 449
BsaJI CCNNGG 5 cut(s) 187, 361, 476, 611, 709
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 1 cut(s) 658
Bse118I RCCGGY 2 cut(s) 269, 585
Bse1I ACTGG 1 cut(s) 132
Bse21I CCTNAGG 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 2 cut(s) 83, 612
BseDI CCNNGG 5 cut(s) 187, 361, 476, 611, 709
BseGI GGATG 3 cut(s) 16, 508, 637
BseLI CCNNNNNNNGG 1 cut(s) 658
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 4 cut(s) 94, 97, 100, 269
BseSI GKGCMC 1 cut(s) 618
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 2 cut(s) 707, 715
BsgI GTGCAG 1 cut(s) 310
Bsh1236I CGCG 1 cut(s) 591
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 2 cut(s) 86, 192
BshNI GGYRCC 2 cut(s) 272, 416
BsiEI CGRYCG 1 cut(s) 193
BsiSI CCGG 4 cut(s) 8, 270, 476, 586
BslI CCNNNNNNNGG 1 cut(s) 658
BsnI GGCC 2 cut(s) 86, 192
Bsp1286I GDGCHC 1 cut(s) 618
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 3 cut(s) 4, 70, 366
Bsp19I CCATGG 1 cut(s) 709
BspACI CCGC 6 cut(s) 99, 166, 193, 303, 313, 464
BspANI GGCC 2 cut(s) 86, 192
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 1 cut(s) 591
BspLI GGNNCC 4 cut(s) 6, 124, 274, 418
BspPI GGATC 3 cut(s) 12, 65, 374
BspT107I GGYRCC 2 cut(s) 272, 416
BsrFI RCCGGY 2 cut(s) 269, 585
BsrI ACTGG 1 cut(s) 132
BssAI RCCGGY 2 cut(s) 269, 585
BssECI CCNNGG 5 cut(s) 187, 361, 476, 611, 709
BssMI GATC 3 cut(s) 4, 70, 366
BssNI GRCGYC 3 cut(s) 219, 417, 449
BssSI CACGAG 1 cut(s) 617
BssT1I CCWWGG 2 cut(s) 361, 709
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 83, 612
Bst4CI ACNGT 3 cut(s) 334, 407, 487
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 3 cut(s) 219, 417, 449
BstDEI CTNAG 1 cut(s) 498
BstDSI CCRYGG 2 cut(s) 187, 709
BstF5I GGATG 3 cut(s) 16, 508, 637
BstFNI CGCG 1 cut(s) 591
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 2 cut(s) 419, 591
BstKTI GATC 3 cut(s) 7, 73, 369
BstMBI GATC 3 cut(s) 4, 70, 366
BstMCI CGRYCG 1 cut(s) 193
BstNI CCWGG 2 cut(s) 83, 612
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 3 cut(s) 81, 475, 610
BstSLI GKGCMC 1 cut(s) 618
BstUI CGCG 1 cut(s) 591
BstV1I GCAGC 2 cut(s) 707, 715
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 2 cut(s) 4, 366
BstXI CCANNNNNNTGG 2 cut(s) 26, 716
BstYI RGATCY 2 cut(s) 4, 366
BstZI CGGCCG 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 498
BsuRI GGCC 2 cut(s) 86, 192
BtgI CCRYGG 2 cut(s) 187, 709
BtrI CACGTC 2 cut(s) 355, 493
BtsCI GGATG 3 cut(s) 16, 508, 637
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 1 cut(s) 682
CfoI GCGC 2 cut(s) 419, 591
Cfr10I RCCGGY 2 cut(s) 269, 585
Cfr13I GGNCC 2 cut(s) 295, 583
CpoI CGGWCCG 1 cut(s) 583
CseI GACGC 2 cut(s) 208, 457
Csp6I GTAC 2 cut(s) 55, 433
CspI CGGWCCG 1 cut(s) 583
CviAII CATG 6 cut(s) 346, 539, 565, 710, 724, 732
CviQI GTAC 2 cut(s) 55, 433
DdeI CTNAG 1 cut(s) 498
DinI GGCGCC 1 cut(s) 418
DpnI GATC 3 cut(s) 6, 72, 368
DpnII GATC 3 cut(s) 4, 70, 366
DraI TTTAAA 1 cut(s) 412
DriI GACNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 190
EagI CGGCCG 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 159
Eam1105I GACNNNNNGTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 2 cut(s) 88, 155
EclXI CGGCCG 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 361, 709
Eco47I GGWCC 2 cut(s) 295, 583
Eco52I CGGCCG 1 cut(s) 190
Eco81I CCTNAGG 1 cut(s) 498
EcoRI GAATTC 1 cut(s) 700
EcoRII CCWGG 2 cut(s) 81, 610
EcoT14I CCWWGG 2 cut(s) 361, 709
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 2 cut(s) 361, 709
FaeI CATG 6 cut(s) 349, 542, 568, 713, 727, 735
FatI CATG 6 cut(s) 345, 538, 564, 709, 723, 731
FauI CCCGC 1 cut(s) 310
Fnu4HI GCNGC 3 cut(s) 193, 721, 729
FokI GGATG 3 cut(s) 23, 515, 644
Fsp4HI GCNGC 3 cut(s) 193, 721, 729
FspBI CTAG 1 cut(s) 362
GlaI GCGC 2 cut(s) 418, 590
GluI GCNGC 3 cut(s) 193, 721, 729
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 2 cut(s) 86, 192
HapII CCGG 4 cut(s) 8, 270, 476, 586
HgaI GACGC 2 cut(s) 208, 457
HhaI GCGC 2 cut(s) 419, 591
Hin1I GRCGYC 3 cut(s) 219, 417, 449
Hin1II CATG 6 cut(s) 349, 542, 568, 713, 727, 735
Hin6I GCGC 2 cut(s) 417, 589
HinP1I GCGC 2 cut(s) 417, 589
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 3 cut(s) 23, 30, 349
HpaII CCGG 4 cut(s) 8, 270, 476, 586
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 3 cut(s) 55, 256, 490
Hpy188I TCNGA 2 cut(s) 583, 699
Hpy188III TCNNGA 6 cut(s) 8, 27, 74, 223, 289, 445
Hpy8I GTNNAC 3 cut(s) 55, 256, 490
Hpy99I CGWCG 2 cut(s) 204, 212
HpyAV CCTTC 4 cut(s) 153, 308, 448, 616
HpyCH4III ACNGT 3 cut(s) 334, 407, 487
HpyCH4IV ACGT 2 cut(s) 354, 492
HpyCH4V TGCA 5 cut(s) 117, 327, 675, 723, 731
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 2 cut(s) 354, 492
Hsp92I GRCGYC 3 cut(s) 219, 417, 449
Hsp92II CATG 6 cut(s) 349, 542, 568, 713, 727, 735
HspAI GCGC 2 cut(s) 417, 589
KasI GGCGCC 1 cut(s) 416
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 3 cut(s) 4, 70, 366
LmnI GCTCC 2 cut(s) 128, 377
Lsp1109I GCAGC 2 cut(s) 707, 715
LweI GCATC 1 cut(s) 126
MaeI CTAG 1 cut(s) 362
MaeII ACGT 2 cut(s) 354, 492
MaeIII GTNAC 3 cut(s) 334, 445, 592
MalI GATC 3 cut(s) 6, 72, 368
MboI GATC 3 cut(s) 4, 70, 366
MboII GAAGA 6 cut(s) 89, 138, 146, 379, 382, 439
MflI RGATCY 2 cut(s) 4, 366
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 2 cut(s) 666, 700
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 2 cut(s) 17, 343
MmeI TCCRAC 1 cut(s) 150
MroI TCCGGA 1 cut(s) 7
MseI TTAA 1 cut(s) 411
MspI CCGG 4 cut(s) 8, 270, 476, 586
MspR9I CCNGG 3 cut(s) 83, 477, 612
MvaI CCWGG 2 cut(s) 83, 612
MvnI CGCG 1 cut(s) 591
NarI GGCGCC 1 cut(s) 417
NciI CCSGG 1 cut(s) 477
NcoI CCATGG 1 cut(s) 709
NdeII GATC 3 cut(s) 4, 70, 366
NlaIII CATG 6 cut(s) 349, 542, 568, 713, 727, 735
NlaIV GGNNCC 4 cut(s) 6, 124, 274, 418
NmuCI GTSAC 3 cut(s) 334, 445, 592
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 1 cut(s) 30
PkrI GCNGC 3 cut(s) 194, 722, 730
PleI GAGTC 2 cut(s) 17, 343
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 2 cut(s) 17, 343
PshAI GACNNNNGTC 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 81, 610
PspGI CCWGG 2 cut(s) 81, 610
PspN4I GGNNCC 4 cut(s) 6, 124, 274, 418
PspPI GGNCC 2 cut(s) 295, 583
PsuI RGATCY 2 cut(s) 4, 366
RsaI GTAC 2 cut(s) 56, 434
RsaNI GTAC 2 cut(s) 55, 433
Rsr2I CGGWCCG 1 cut(s) 583
RsrII CGGWCCG 1 cut(s) 583
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 3 cut(s) 193, 721, 729
Sau3AI GATC 3 cut(s) 4, 70, 366
Sau96I GGNCC 2 cut(s) 295, 583
SchI GAGTC 2 cut(s) 17, 343
ScrFI CCNGG 3 cut(s) 83, 477, 612
SduI GDGCHC 1 cut(s) 618
SetI ASST 6 cut(s) 145, 245, 357, 495, 512, 639
SfaNI GCATC 1 cut(s) 126
SfoI GGCGCC 1 cut(s) 418
SinI GGWCC 2 cut(s) 295, 583
Sse9I AATT 2 cut(s) 666, 700
SsiI CCGC 6 cut(s) 99, 166, 193, 303, 313, 464
SspDI GGCGCC 1 cut(s) 416
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 3 cut(s) 81, 475, 610
StyI CCWWGG 2 cut(s) 361, 709
TaaI ACNGT 3 cut(s) 334, 407, 487
TaiI ACGT 2 cut(s) 357, 495
TaqI TCGA 1 cut(s) 288
TasI AATT 2 cut(s) 666, 700
TatI WGTACW 1 cut(s) 432
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 3 cut(s) 334, 445, 592
TseI GCWGC 2 cut(s) 720, 728
Tsp45I GTSAC 3 cut(s) 334, 445, 592
TspDTI ATGAA 4 cut(s) 27, 138, 527, 553
TspGWI ACGGA 2 cut(s) 542, 611
TspRI CASTG 1 cut(s) 682
VpaK11BI GGWCC 2 cut(s) 295, 583
XapI RAATTY 1 cut(s) 700
XmaJI CCTAGG 1 cut(s) 361
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.