Rw2G049990

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014103
Physical Location & Seq
Reverse (-)
77526540 .. 77527332
793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049990.1

Sequence Viewer

Length: 672 bp
ATGGATCCGGATCAACCCCAAGACTCTGGAATCAAAATCTACAACAACGCCGTGTACGGCGACCCACCCGACCCCGTGGACCAGGCCAAGCCCAAGTCCGCCTCCTCCTCCTCCTGCATCGGCTCCAACCACAGTAAGCCACCTTCTTCATCTCTCCCTCTCGTCCGCCTCAACCACGACCAACTCCACGGCCGCAAACGACGTGCCGTCGCCAAAGGCGTCCAGAAAACCATCTCCAAAACCTCTATGCTCGTCAACTTCCTCCCCACCGGAACCCTCCTCACTTTCGAGATGGTCCTCCCCGCCATCTACCGCAACGGCGAATGCACCGCCGGATCTGCTCCCTCTCCTGCTCTTCTTCCACTTCACCGACGTTTTAAAGGCGCCGATGGGAAGATGTACTACGGCTTCGTGACGCCGAAGGGGTTGGCGGTTTTCAAGCCGGGGCTGACGGTGGACGTGCCTAAGGATGAGAGGTATAGGGTGGGGCTGACGGACTTTGTTCATGCCATAATGTCAGTGATGGTGTTCACGGCGATTGCGTTTTCGGATCGGCGTGTGACGGACTGTAATGGAGAGTTTCCGCTGATGGTGGGTATTGTTTGCAGTGGTTTGTTCTTGTGTTTCCGAATTTCTCGCCATGGGATTGGCTGCATGGCCGCATGTTGTTAA

Protein Analysis

223

Amino Acids

24.15

Weight (kDa)

9.25

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 80 - 218 4.5e-31 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09157 AT5G39650
fragaria_vesca FvH4_6g48440
malus_domestica MD00G1130800.v1.1 MD09G1051900.v1.1
prunus_persica Prupe.3G266800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0168351
rosa_laevigata RLG00000021769
rosa_multiflora Rmu_sc0003354.1_g000017
rosa_roxburghii Rroxscaffold_2G00083120
rosa_rugosa Rorug02G0533100
rosa_samantha Rh2AG602200 Rh2BG615500 Rh2CG583200 Rh2DG625700
rosa_wichuraiana Rw0G019820 Rw2G049990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 9 cut(s) 99, 166, 193, 303, 313, 330, 431, 584, 660
AclWI GGATC 4 cut(s) 12, 18, 343, 558
AcoI YGGCCR 2 cut(s) 190, 657
AcsI RAATTY 1 cut(s) 630
AcyI GRCGYC 3 cut(s) 219, 384, 416
AfaI GTAC 2 cut(s) 56, 401
AgsI TTSAA 1 cut(s) 439
AhdI GACNNNNNGTC 1 cut(s) 206
AjiI CACGTC 2 cut(s) 203, 460
AjnI CCWGG 1 cut(s) 81
AlwI GGATC 4 cut(s) 12, 18, 343, 558
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 3 cut(s) 84, 190, 657
ApeKI GCWGC 1 cut(s) 651
ApoI RAATTY 1 cut(s) 630
AspLEI GCGC 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 79, 295
AsuC2I CCSGG 1 cut(s) 444
AsuHPI GGTGA 1 cut(s) 359
AvaII GGWCC 2 cut(s) 79, 295
AxyI CCTNAGG 1 cut(s) 465
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 383
BarI GAAGNNNNNNTAC 6 cut(s) 127, 159, 386, 392, 418, 424
BbvI GCAGC 1 cut(s) 638
BccI CCATC 6 cut(s) 239, 286, 314, 383, 517, 583
BceAI ACGGC 7 cut(s) 35, 73, 191, 205, 334, 421, 549
BciT130I CCWGG 1 cut(s) 83
BcnI CCSGG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 387
BisI GCNGC 3 cut(s) 193, 652, 660
BlsI GCNGC 3 cut(s) 194, 653, 661
Bme1390I CCNGG 2 cut(s) 83, 444
Bme18I GGWCC 2 cut(s) 79, 295
BmeRI GACNNNNNGTC 1 cut(s) 206
BmgBI CACGTC 2 cut(s) 203, 460
BmgT120I GGNCC 2 cut(s) 79, 295
BmiI GGNNCC 4 cut(s) 6, 124, 274, 385
BmrFI CCNGG 2 cut(s) 83, 444
BmsI GCATC 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 444
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 3 cut(s) 219, 384, 416
BsaJI CCNNGG 4 cut(s) 75, 187, 443, 640
BsaWI WCCGGW 2 cut(s) 7, 269
Bse21I CCTNAGG 1 cut(s) 465
Bse8I GATNNNNATC 1 cut(s) 9
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 4 cut(s) 75, 187, 443, 640
BseGI GGATG 1 cut(s) 475
BseJI GATNNNNATC 1 cut(s) 9
BseRI GAGGAG 4 cut(s) 94, 97, 100, 269
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 1 cut(s) 638
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 3 cut(s) 86, 192, 659
BshNI GGYRCC 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 193
BsiSI CCGG 4 cut(s) 8, 270, 333, 443
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 3 cut(s) 86, 192, 659
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 4 cut(s) 4, 10, 335, 550
Bsp19I CCATGG 1 cut(s) 640
BspACI CCGC 9 cut(s) 99, 166, 193, 303, 313, 330, 431, 584, 660
BspANI GGCC 3 cut(s) 86, 192, 659
BspEI TCCGGA 1 cut(s) 7
BspLI GGNNCC 4 cut(s) 6, 124, 274, 385
BspPI GGATC 4 cut(s) 12, 18, 343, 558
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 383
BssECI CCNNGG 4 cut(s) 75, 187, 443, 640
BssMI GATC 4 cut(s) 4, 10, 335, 550
BssNI GRCGYC 3 cut(s) 219, 384, 416
BssT1I CCWWGG 1 cut(s) 640
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 3 cut(s) 134, 454, 569
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 3 cut(s) 219, 384, 416
BstDEI CTNAG 1 cut(s) 465
BstDSI CCRYGG 3 cut(s) 75, 187, 640
BstF5I GGATG 1 cut(s) 475
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 4 cut(s) 7, 13, 338, 553
BstMBI GATC 4 cut(s) 4, 10, 335, 550
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 81, 442
BstV1I GCAGC 1 cut(s) 638
BstX2I RGATCY 2 cut(s) 4, 335
BstXI CCANNNNNNTGG 2 cut(s) 26, 647
BstYI RGATCY 2 cut(s) 4, 335
BstZI CGGCCG 1 cut(s) 190
Bsu36I CCTNAGG 1 cut(s) 465
BsuRI GGCC 3 cut(s) 86, 192, 659
BtgI CCRYGG 3 cut(s) 75, 187, 640
BtrI CACGTC 2 cut(s) 203, 460
BtsCI GGATG 1 cut(s) 475
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 525, 613
CfoI GCGC 1 cut(s) 386
Cfr13I GGNCC 2 cut(s) 79, 295
CseI GACGC 2 cut(s) 208, 424
Csp6I GTAC 2 cut(s) 55, 400
CviAII CATG 4 cut(s) 506, 641, 655, 663
CviQI GTAC 2 cut(s) 55, 400
DdeI CTNAG 1 cut(s) 465
DinI GGCGCC 1 cut(s) 385
DpnI GATC 4 cut(s) 6, 12, 337, 552
DpnII GATC 4 cut(s) 4, 10, 335, 550
DraI TTTAAA 1 cut(s) 379
DriI GACNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 2 cut(s) 190, 657
EagI CGGCCG 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 360
Eam1105I GACNNNNNGTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 2 cut(s) 88, 155
EclXI CGGCCG 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 79, 295
Eco52I CGGCCG 1 cut(s) 190
Eco81I CCTNAGG 1 cut(s) 465
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 640
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 640
FaeI CATG 4 cut(s) 509, 644, 658, 666
FaiI YATR 7 cut(s) 248, 480, 507, 512, 642, 656, 664
FatI CATG 4 cut(s) 505, 640, 654, 662
FauI CCCGC 1 cut(s) 310
Fnu4HI GCNGC 3 cut(s) 193, 652, 660
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 3 cut(s) 193, 652, 660
GlaI GCGC 1 cut(s) 385
GluI GCNGC 3 cut(s) 193, 652, 660
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 3 cut(s) 86, 192, 659
HapII CCGG 4 cut(s) 8, 270, 333, 443
HgaI GACGC 2 cut(s) 208, 424
HhaI GCGC 1 cut(s) 386
Hin1I GRCGYC 3 cut(s) 219, 384, 416
Hin1II CATG 4 cut(s) 509, 644, 658, 666
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 2 cut(s) 23, 30
HpaII CCGG 4 cut(s) 8, 270, 333, 443
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 5 cut(s) 55, 79, 256, 457, 531
Hpy188I TCNGA 2 cut(s) 550, 629
Hpy188III TCNNGA 5 cut(s) 8, 27, 223, 289, 412
Hpy8I GTNNAC 5 cut(s) 55, 79, 256, 457, 531
Hpy99I CGWCG 3 cut(s) 204, 212, 375
HpyAV CCTTC 2 cut(s) 153, 415
HpyCH4III ACNGT 3 cut(s) 134, 454, 569
HpyCH4IV ACGT 3 cut(s) 202, 373, 459
HpyCH4V TGCA 4 cut(s) 117, 327, 606, 654
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 3 cut(s) 202, 373, 459
Hsp92I GRCGYC 3 cut(s) 219, 384, 416
Hsp92II CATG 4 cut(s) 509, 644, 658, 666
HspAI GCGC 1 cut(s) 384
KasI GGCGCC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 4 cut(s) 4, 10, 335, 550
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 128, 346
Lsp1109I GCAGC 1 cut(s) 638
LweI GCATC 1 cut(s) 126
MaeII ACGT 3 cut(s) 202, 373, 459
MaeIII GTNAC 2 cut(s) 412, 559
MalI GATC 4 cut(s) 6, 12, 337, 552
MboI GATC 4 cut(s) 4, 10, 335, 550
MboII GAAGA 4 cut(s) 138, 347, 350, 406
MflI RGATCY 2 cut(s) 4, 335
MluCI AATT 1 cut(s) 630
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 150
MroI TCCGGA 1 cut(s) 7
MseI TTAA 2 cut(s) 378, 670
MspA1I CMGCKG 1 cut(s) 586
MspI CCGG 4 cut(s) 8, 270, 333, 443
MspR9I CCNGG 2 cut(s) 83, 444
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 338
NarI GGCGCC 1 cut(s) 384
NciI CCSGG 1 cut(s) 444
NcoI CCATGG 1 cut(s) 640
NdeII GATC 4 cut(s) 4, 10, 335, 550
NlaIII CATG 4 cut(s) 509, 644, 658, 666
NlaIV GGNNCC 4 cut(s) 6, 124, 274, 385
NmuCI GTSAC 2 cut(s) 412, 559
NspI RCATGY 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 360
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 539
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 30
PkrI GCNGC 3 cut(s) 194, 653, 661
PleI GAGTC 1 cut(s) 17
PluTI GGCGCC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 4 cut(s) 6, 124, 274, 385
PspPI GGNCC 2 cut(s) 79, 295
PsuI RGATCY 2 cut(s) 4, 335
RsaI GTAC 2 cut(s) 56, 401
RsaNI GTAC 2 cut(s) 55, 400
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 2 cut(s) 378, 670
SatI GCNGC 3 cut(s) 193, 652, 660
Sau3AI GATC 4 cut(s) 4, 10, 335, 550
Sau96I GGNCC 2 cut(s) 79, 295
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 83, 444
SetI ASST 6 cut(s) 145, 205, 245, 376, 462, 479
SfaNI GCATC 1 cut(s) 126
SfoI GGCGCC 1 cut(s) 385
SinI GGWCC 2 cut(s) 79, 295
Sse9I AATT 1 cut(s) 630
SsiI CCGC 9 cut(s) 99, 166, 193, 303, 313, 330, 431, 584, 660
SspDI GGCGCC 1 cut(s) 383
StyD4I CCNGG 2 cut(s) 81, 442
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 3 cut(s) 134, 454, 569
TaiI ACGT 3 cut(s) 205, 376, 462
TaqI TCGA 1 cut(s) 288
TasI AATT 1 cut(s) 630
TatI WGTACW 1 cut(s) 399
TauI GCSGC 2 cut(s) 195, 662
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 2 cut(s) 378, 670
Tru9I TTAA 2 cut(s) 378, 670
TscAI CASTG 2 cut(s) 525, 613
TseFI GTSAC 2 cut(s) 412, 559
TseI GCWGC 1 cut(s) 651
Tsp45I GTSAC 2 cut(s) 412, 559
TspDTI ATGAA 2 cut(s) 138, 494
TspGWI ACGGA 2 cut(s) 509, 578
TspRI CASTG 2 cut(s) 525, 613
VpaK11BI GGWCC 2 cut(s) 79, 295
XapI RAATTY 1 cut(s) 630
XceI RCATGY 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.