Rroxscaffold_2G00083120

Protein of unknown function (DUF679)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014103
Physical Location & Seq
Forward (+)
5862691 .. 5863416
726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083120.1

Sequence Viewer

Length: 726 bp
ATGGATCCGGATCAACCCCAAGACTCTGGAATCAAAATCTACAACAACGCCGTGTACGGCGACCCACCCGATCCAGAGGACCAGGCCAAGCCCAAGTCCGCCTCCTCCTCCTCCTGCATCGGCTCCAACCCACCTTCTTCAGCTCTCCCTCTCGTCCGCCTCAACCACGACCAACTCCACGGCCGCAAACGACGCGCCGTCGCCAAAGGCGTCCAGAAAACCATCTCCAAAACCTCTATGCTCGTCAACTTCCTCCCCACCGGCACCCTCCTCACTTTCGAGATGGTCCTCCCCGCGATCTACCGCAACGGCGAATGCACCACCGTCACTACCACCATGACTCACGTCCTCCTAGGGATCTGCTCCCTCTCCTGCTTCTTCTTCCACTTCACCGACAGTTTTAAAGGCGCCGATGGGAAGATGTACTACGGCTTCGTGACGCCGAAGGGGTTGGCGGTTTTCAAGCCGGGGCTGACGGTGGACGTGCCTAAGGATGAAAGGTATAGGGTGGGGCTGACGGACTTTGTTCATGCCATAATGTCAGTGATGGTGTTCATGGCGATTGCGTTTTCGGATCGGCGTGTGACGGAGTGTATGTTTCCCGGGCACGAGAAGGAGATGGATGAGGTAATGGAGAGTTTCCCATTGATGGTGGGAATTGTTTGCAGTGGCTTGTTTCTTGTGTTTCCGAATTCTCGCCATGGGATTGGCTGCATGGCTGCATGA

Protein Analysis

241

Amino Acids

26.43

Weight (kDa)

7.03

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF679 PF05078 77 - 238 9.5e-66 Protein of unknown function (DUF679)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09157 AT5G39650
fragaria_vesca FvH4_6g48440
malus_domestica MD00G1130800.v1.1 MD09G1051900.v1.1
prunus_persica Prupe.3G266800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0168351
rosa_laevigata RLG00000021769
rosa_multiflora Rmu_sc0003354.1_g000017
rosa_roxburghii Rroxscaffold_2G00083120
rosa_rugosa Rorug02G0533100
rosa_samantha Rh2AG602200 Rh2BG615500 Rh2CG583200 Rh2DG625700
rosa_wichuraiana Rw0G019820 Rw2G049990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 263, 407
AccII CGCG 2 cut(s) 195, 296
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 6 cut(s) 99, 157, 184, 294, 304, 455
AclWI GGATC 5 cut(s) 12, 18, 65, 365, 582
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 1 cut(s) 123
AcyI GRCGYC 3 cut(s) 210, 408, 440
AfaI GTAC 2 cut(s) 56, 425
AfiI CCNNNNNNNGG 1 cut(s) 649
AgsI TTSAA 1 cut(s) 463
AhdI GACNNNNNGTC 1 cut(s) 197
AjiI CACGTC 2 cut(s) 346, 484
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 1 cut(s) 143
AluI AGCT 1 cut(s) 143
AlwI GGATC 5 cut(s) 12, 18, 65, 365, 582
Ama87I CYCGRG 1 cut(s) 602
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 84, 181
ApeKI GCWGC 2 cut(s) 711, 719
ApoI RAATTY 1 cut(s) 691
AspA2I CCTAGG 1 cut(s) 352
AspLEI GCGC 2 cut(s) 197, 410
AspS9I GGNCC 2 cut(s) 79, 286
AsuC2I CCSGG 3 cut(s) 468, 603, 604
AsuHPI GGTGA 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 2 cut(s) 79, 286
AvrII CCTAGG 1 cut(s) 352
AxyI CCTNAGG 1 cut(s) 489
BaeGI GKGCMC 1 cut(s) 609
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 263, 407
BarI GAAGNNNNNNTAC 4 cut(s) 410, 416, 442, 448
BauI CACGAG 1 cut(s) 608
BbvI GCAGC 2 cut(s) 698, 706
BccI CCATC 6 cut(s) 230, 277, 407, 541, 613, 643
BceAI ACGGC 6 cut(s) 35, 73, 182, 196, 325, 445
BciT130I CCWGG 1 cut(s) 83
BcnI CCSGG 3 cut(s) 468, 603, 604
BfaI CTAG 1 cut(s) 353
BfoI RGCGCY 1 cut(s) 411
BisI GCNGC 3 cut(s) 184, 712, 720
BlnI CCTAGG 1 cut(s) 352
BlsI GCNGC 3 cut(s) 185, 713, 721
Bme1390I CCNGG 4 cut(s) 83, 468, 603, 604
Bme18I GGWCC 2 cut(s) 79, 286
BmeRI GACNNNNNGTC 1 cut(s) 197
BmeT110I CYCGRG 1 cut(s) 602
BmgBI CACGTC 2 cut(s) 346, 484
BmgT120I GGNCC 2 cut(s) 79, 286
BmiI GGNNCC 4 cut(s) 6, 124, 265, 409
BmrFI CCNGG 4 cut(s) 83, 468, 603, 604
BmsI GCATC 1 cut(s) 126
BoxI GACNNNNGTC 1 cut(s) 344
BpuMI CCSGG 3 cut(s) 468, 603, 604
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 3 cut(s) 210, 408, 440
BsaJI CCNNGG 5 cut(s) 178, 352, 467, 602, 700
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 1 cut(s) 649
Bse118I RCCGGY 1 cut(s) 260
Bse21I CCTNAGG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 9
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 5 cut(s) 178, 352, 467, 602, 700
BseGI GGATG 2 cut(s) 499, 628
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 649
BseRI GAGGAG 4 cut(s) 94, 97, 100, 260
BseSI GKGCMC 1 cut(s) 609
BseX3I CGGCCG 1 cut(s) 181
BseXI GCAGC 2 cut(s) 698, 706
Bsh1236I CGCG 2 cut(s) 195, 296
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 2 cut(s) 86, 183
BshNI GGYRCC 2 cut(s) 263, 407
BsiEI CGRYCG 1 cut(s) 184
BsiHKCI CYCGRG 1 cut(s) 602
BsiSI CCGG 4 cut(s) 8, 261, 467, 603
BslI CCNNNNNNNGG 1 cut(s) 649
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 2 cut(s) 86, 183
BsoBI CYCGRG 1 cut(s) 602
Bsp1286I GDGCHC 1 cut(s) 609
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 6 cut(s) 4, 10, 70, 297, 357, 574
Bsp19I CCATGG 1 cut(s) 700
BspACI CCGC 6 cut(s) 99, 157, 184, 294, 304, 455
BspANI GGCC 2 cut(s) 86, 183
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 2 cut(s) 195, 296
BspLI GGNNCC 4 cut(s) 6, 124, 265, 409
BspPI GGATC 5 cut(s) 12, 18, 65, 365, 582
BspT107I GGYRCC 2 cut(s) 263, 407
BsrFI RCCGGY 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 5 cut(s) 178, 352, 467, 602, 700
BssMI GATC 6 cut(s) 4, 10, 70, 297, 357, 574
BssNI GRCGYC 3 cut(s) 210, 408, 440
BssSI CACGAG 1 cut(s) 608
BssT1I CCWWGG 2 cut(s) 352, 700
Bst2BI CACGAG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 3 cut(s) 325, 398, 478
BstACI GRCGYC 3 cut(s) 210, 408, 440
BstDEI CTNAG 1 cut(s) 489
BstDSI CCRYGG 2 cut(s) 178, 700
BstF5I GGATG 2 cut(s) 499, 628
BstFNI CGCG 2 cut(s) 195, 296
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 2 cut(s) 197, 410
BstKTI GATC 6 cut(s) 7, 13, 73, 300, 360, 577
BstMBI GATC 6 cut(s) 4, 10, 70, 297, 357, 574
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNI CCWGG 1 cut(s) 83
BstPAI GACNNNNGTC 1 cut(s) 344
BstSCI CCNGG 4 cut(s) 81, 466, 601, 602
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 2 cut(s) 195, 296
BstV1I GCAGC 2 cut(s) 698, 706
BstX2I RGATCY 2 cut(s) 4, 357
BstXI CCANNNNNNTGG 2 cut(s) 26, 707
BstYI RGATCY 2 cut(s) 4, 357
BstZI CGGCCG 1 cut(s) 181
Bsu36I CCTNAGG 1 cut(s) 489
BsuRI GGCC 2 cut(s) 86, 183
BtgI CCRYGG 2 cut(s) 178, 700
BtrI CACGTC 2 cut(s) 346, 484
BtsCI GGATG 2 cut(s) 499, 628
BtsI GCAGTG 1 cut(s) 673
BtsIMutI CAGTG 2 cut(s) 549, 673
CfoI GCGC 2 cut(s) 197, 410
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 2 cut(s) 79, 286
Cfr9I CCCGGG 1 cut(s) 602
CseI GACGC 3 cut(s) 199, 201, 448
Csp6I GTAC 2 cut(s) 55, 424
CviAII CATG 6 cut(s) 337, 530, 556, 701, 715, 723
CviQI GTAC 2 cut(s) 55, 424
DdeI CTNAG 1 cut(s) 489
DinI GGCGCC 1 cut(s) 409
DpnI GATC 6 cut(s) 6, 12, 72, 299, 359, 576
DpnII GATC 6 cut(s) 4, 10, 70, 297, 357, 574
DraI TTTAAA 1 cut(s) 403
DriI GACNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 1 cut(s) 181
EagI CGGCCG 1 cut(s) 181
Eam1105I GACNNNNNGTC 1 cut(s) 197
EciI GGCGGA 2 cut(s) 88, 146
EclXI CGGCCG 1 cut(s) 181
Eco130I CCWWGG 2 cut(s) 352, 700
Eco47I GGWCC 2 cut(s) 79, 286
Eco52I CGGCCG 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 123
Eco81I CCTNAGG 1 cut(s) 489
Eco88I CYCGRG 1 cut(s) 602
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 2 cut(s) 352, 700
EgeI GGCGCC 1 cut(s) 409
EheI GGCGCC 1 cut(s) 409
ErhI CCWWGG 2 cut(s) 352, 700
FaeI CATG 6 cut(s) 340, 533, 559, 704, 718, 726
FatI CATG 6 cut(s) 336, 529, 555, 700, 714, 722
FauI CCCGC 1 cut(s) 301
Fnu4HI GCNGC 3 cut(s) 184, 712, 720
FokI GGATG 2 cut(s) 506, 635
Fsp4HI GCNGC 3 cut(s) 184, 712, 720
FspBI CTAG 1 cut(s) 353
GlaI GCGC 2 cut(s) 196, 409
GluI GCNGC 3 cut(s) 184, 712, 720
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 2 cut(s) 86, 183
HapII CCGG 4 cut(s) 8, 261, 467, 603
HgaI GACGC 3 cut(s) 199, 201, 448
HhaI GCGC 2 cut(s) 197, 410
Hin1I GRCGYC 3 cut(s) 210, 408, 440
Hin1II CATG 6 cut(s) 340, 533, 559, 704, 718, 726
Hin6I GCGC 2 cut(s) 195, 408
HinP1I GCGC 2 cut(s) 195, 408
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 23, 30, 340
HpaII CCGG 4 cut(s) 8, 261, 467, 603
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 3 cut(s) 55, 247, 481
Hpy188I TCNGA 2 cut(s) 574, 690
Hpy188III TCNNGA 6 cut(s) 8, 27, 74, 214, 280, 436
Hpy8I GTNNAC 3 cut(s) 55, 247, 481
Hpy99I CGWCG 2 cut(s) 195, 203
HpyAV CCTTC 3 cut(s) 144, 439, 607
HpyCH4III ACNGT 3 cut(s) 325, 398, 478
HpyCH4IV ACGT 2 cut(s) 345, 483
HpyCH4V TGCA 5 cut(s) 117, 318, 666, 714, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 1 cut(s) 489
HpySE526I ACGT 2 cut(s) 345, 483
Hsp92I GRCGYC 3 cut(s) 210, 408, 440
Hsp92II CATG 6 cut(s) 340, 533, 559, 704, 718, 726
HspAI GCGC 2 cut(s) 195, 408
KasI GGCGCC 1 cut(s) 407
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 6 cut(s) 4, 10, 70, 297, 357, 574
LmnI GCTCC 2 cut(s) 128, 368
Lsp1109I GCAGC 2 cut(s) 698, 706
LweI GCATC 1 cut(s) 126
MaeI CTAG 1 cut(s) 353
MaeII ACGT 2 cut(s) 345, 483
MaeIII GTNAC 3 cut(s) 325, 436, 583
MalI GATC 6 cut(s) 6, 12, 72, 299, 359, 576
MboI GATC 6 cut(s) 4, 10, 70, 297, 357, 574
MboII GAAGA 4 cut(s) 129, 370, 373, 430
MflI RGATCY 2 cut(s) 4, 357
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 2 cut(s) 657, 691
Mly113I GGCGCC 1 cut(s) 408
MlyI GAGTC 2 cut(s) 17, 334
MmeI TCCRAC 1 cut(s) 150
MroI TCCGGA 1 cut(s) 7
MseI TTAA 1 cut(s) 402
MspI CCGG 4 cut(s) 8, 261, 467, 603
MspR9I CCNGG 4 cut(s) 83, 468, 603, 604
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 2 cut(s) 195, 296
MwoI GCNNNNNNNGC 1 cut(s) 192
NarI GGCGCC 1 cut(s) 408
NciI CCSGG 3 cut(s) 468, 603, 604
NcoI CCATGG 1 cut(s) 700
NdeII GATC 6 cut(s) 4, 10, 70, 297, 357, 574
NlaIII CATG 6 cut(s) 340, 533, 559, 704, 718, 726
NlaIV GGNNCC 4 cut(s) 6, 124, 265, 409
NmuCI GTSAC 3 cut(s) 325, 436, 583
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 320
PfeI GAWTC 1 cut(s) 30
PkrI GCNGC 3 cut(s) 185, 713, 721
PleI GAGTC 2 cut(s) 17, 334
PluTI GGCGCC 1 cut(s) 411
PpsI GAGTC 2 cut(s) 17, 334
PshAI GACNNNNGTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 4 cut(s) 6, 124, 265, 409
PspPI GGNCC 2 cut(s) 79, 286
PsuI RGATCY 2 cut(s) 4, 357
RsaI GTAC 2 cut(s) 56, 425
RsaNI GTAC 2 cut(s) 55, 424
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 3 cut(s) 184, 712, 720
Sau3AI GATC 6 cut(s) 4, 10, 70, 297, 357, 574
Sau96I GGNCC 2 cut(s) 79, 286
SchI GAGTC 2 cut(s) 17, 334
ScrFI CCNGG 4 cut(s) 83, 468, 603, 604
SduI GDGCHC 1 cut(s) 609
SetI ASST 7 cut(s) 136, 145, 236, 348, 486, 503, 630
SfaNI GCATC 1 cut(s) 126
SfoI GGCGCC 1 cut(s) 409
SinI GGWCC 2 cut(s) 79, 286
SmaI CCCGGG 1 cut(s) 604
Sse9I AATT 2 cut(s) 657, 691
SsiI CCGC 6 cut(s) 99, 157, 184, 294, 304, 455
SspDI GGCGCC 1 cut(s) 407
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 4 cut(s) 81, 466, 601, 602
StyI CCWWGG 2 cut(s) 352, 700
TaaI ACNGT 3 cut(s) 325, 398, 478
TaiI ACGT 2 cut(s) 348, 486
TaqI TCGA 1 cut(s) 279
TasI AATT 2 cut(s) 657, 691
TatI WGTACW 1 cut(s) 423
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 1 cut(s) 30
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 2 cut(s) 549, 673
TseFI GTSAC 3 cut(s) 325, 436, 583
TseI GCWGC 2 cut(s) 711, 719
Tsp45I GTSAC 3 cut(s) 325, 436, 583
TspDTI ATGAA 3 cut(s) 510, 518, 544
TspGWI ACGGA 2 cut(s) 533, 602
TspMI CCCGGG 1 cut(s) 602
TspRI CASTG 2 cut(s) 549, 673
VpaK11BI GGWCC 2 cut(s) 79, 286
XapI RAATTY 1 cut(s) 691
XmaI CCCGGG 1 cut(s) 602
XmaJI CCTAGG 1 cut(s) 352
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.