Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0009778

Physical Location & Seq

Genomic Coordinates Forward (+)

78446246 .. 78453078

Gene Structure 6833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021808

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3837 bp

ATGGCTGACACCACAGAGCCAACGAAGACATTGCCGGAGGCAGAGAAAAAGAAAGAACAGAGCTTGCCGTTTTACCAGCTCTTCTCCTTTGCTGACAAGTACGATTGCCTTCTCATGGTCTCTGGCAGCATAGGAGCCATAATTCACGGCTCTTCTATGCCCGTGTTCTTCCTCTTATTTGGTGAAATGGTTAATGGGTTTGGCAAAAACCAAATGAATCTGCAGAAAATGACCGCCGAAGTAGCCAAGTATGCTCTGTATTTTGTGTATCTCGGCCTGATCGTCTGCTTCTCATCCTACGCCGAGATTGCATGCTGGATGTACACCGGAGAAAGACAAGTGAGCACTCTGAGGAAGAAGTATCTGGAAGCCGTGCTTAAACAAGACGTGGGGTTTTTCGACACAGATGCCAGAACAGGTGACATAGTCTTCAGCGTCTCGACGGACACCCTTCTGGTCCAAGATGCCATTAGCGAGAAGGTGGGGAACTTCATACACTATCTCTCAACATTTCTGGCGGGACTGGTGGTTGGGTTTGTATCAGCATGGAGGCTGGCGCTGCTGAGTGTGGCAGTCATCCCGGGGATTGCTTTTGCTGGCGGGTTGTATGCTTATACGCTTACAGGGCTCACATCCAAGAGCCGAGAATCGTATGCAAATGCAGGCATTATGGCTGAGCAAGCCATTGCACAAGTACGAACAGTTAAATCATATGTTGGTGAAAGCAAGGCCCTGAATTCGTATTCAGATGCCATACAGAACACCTTGCAGCTTGGTTACAAGGCCGGAATGGCCAAAGGTCTGGGGTTAGGGTGTACCTATGGCATAGCTTGTATGTCGTGGGCTCTTGTTTTCTGGTATGCCGGTGTGTTCATCCGAAATGGACAGACTGATGGAGGGAAAGCATTCACAGCCATTTTTTCAGCTATTGTTGGTGGCATGAGCTTGGGTCAGTCATTTTCCAATCTTGGGGCCTTTAGCAAAGGTAAATCAGCTGGATACAAGTTGATGGAGATTATTAGGCAAAAGCCCACCATAATTCAGGACCAGTTGGATGGAAAGTGCTTGTCTGAGGTTAAAGGAGACATAGAACTCAAGGAGGTGACATTCAGCTATCCATCCAGGCCAGATGTTATCATTTTCCGAAATTTCTCGATCTTCTTCCCAGCTGGAAAGACTGTTGCCGTTGTCGGCGGCAGTGGTTCAGGAAAAAGCACCGTTGTCTCTCTGATTGAAAGGTTTTACGATCCTAACCAGGGTAAGATGGAGACTTTTCACATTTTACCGGCATTGATTGAAAGGGTTATATCATATTTGCTAATATTTCTCGTTGGCTTAGCAGGGCAGGTTTTGCTGGATGGTGTGGACATAAGAACACTGCAACTGAAATGGTTGCGTGATCAGATGGGCCTGGTAAACCAGGAGCCTGCACTCTTTGCTACTACCATTCTTGAGAACATACTCTATGGAAAGCCTGATGCAACAATGGATGAAGTAGAAGCTGCAGCCTCTGCAGCTAATGCTCATAGTTTCATCACTTTGCTTCCTAATGGGTATAACACTCAGGTCGGAGAGCGAGGAGTCCAACTCTCTGGAGGTCAAAAACAGAGAATTGCAATTGCTAGAGCTATGTTGAAAGACCCAAAGATCCTGCTCCTTGATGAAGCAACCAGCGCCCTTGATGCAAGCTCTGAGAGCATTGTACAAGAAGCTCTAGATCGTCTCATGGTTGGGAGGACAACTGTAGTTGTGGCACATCGCCTTTCCACCATAAGGAATGTTGACAGCATTGCAGTTATACAACAAGGGCAAGTTGTTGAGACGGGAACTCATGAAGAACTGATTGCCAAAGCAGGGGCTTATTCTTCATTAATTCGGTTCCAAGAAATGGTTGGAAACAGAGACTTCCGGAATCCATCTACTCGTTGTTCACGATCATCACGACTAAGCCACTCATTGTCAACCAAGTCTTTGAGCCTCCGGTCTGGCAGCTTAAGGAACCTGAGCTACCAGTACAGTACTGGTGCTGATGGGAGGATAGAGATGATTTCAAATGCTGAGACAGACAGGAAAACTCGAGCTCCAAGGGGATATTTCTTTCGGCTTCTCACGCTAAATGCTCCTGAATGGCCTTATTCAATCATGGGTGCCATAGGATCAGTACTATCCGGGTTTATCGGTCCAACCTTTGCTATTGTAATGAGCAACATGATTGAGGTGTTCTATTATAGAAATCCTGCTTCCATGGAGAGGAAGACAAAGGAGTATGTCTTCATTTACATAGGAGCTGGGCTCTATGCTGTAGTTGCATATTTGATACAACATTACTTCTTCAGCATAATGGGAGAGAACCTCACTACTAGAGTAAGGAGGATGATGCTAGCAGCAATTCTAAGGAACGAAGTCGGATGGTATGATGAAGAGGAGAACAACTCGAGTCTTCTAGCTTCAAAACTGGCCACAGATGCTGCTGATGTGAAATCCGCAATTGCTGAGAGGATTTCTGTGATTCTGCAGAACATGACTTCACTGCTCACTTCATTCATAGTTGCTTTCATTGTGGAGTGGAGGGTCTCCCTGCTTATCCTAGCAACCTTTCCTCTTCTAGTTCTTGCCAACTTTGCTCAGCAACTTTCGCTTAAAGGTTTTGCCGGAGACACTGCCAAGGCTCATGCAAAGACGAGCATGATTGCAGGGGAAGGAGTGAGCAACATTCGAACAGTTGCAGCCTTCAATGCACAAGACAAGATCCTCTCTCTCTTCTGCCATGAGCTTCGTATCCCACAACTAGGAAGCCTTCGTCGGAGCCAAACTGCTGGTCTCCTATTTGGCCTCTCACAACTTGCGTTATATGCTTCTGAAGCTCTCATTCTATGGTACGGTGCCCACCTTGTGAGCAAAGGTGTCTCAACCTTCTCCAAAGTCATCAAGGTTTTTGTGGTCCTGGTCGTAACAGCCAATTCAGTAGCAGAAACAGTCAGTCTTGCCCCTGAGATTATTAGGGGTGGTGAAGCTGTTGGCTCTGTGTTTTCAATTCTTGATCGCCAAACTAGGATTGACCCGGATGACCCTGAGGCTGAGGTGGTTGAAACAATTCGTGGGGAAATTGAACTTAGGCATGTCGATTTTGCATACCCTTCACGACCTGATATTATGATCTTTAAAGATTTTAATCTCAGAATCCGTACTGGCCAAAGCCAAGCACTTGTTGGAGCTAGTGGTTCGGGGAAGAGTTCCGTGATTGCATTGATCGAGAGGTTCTATGATCCAATAGTTGGGAAGGTAATGATTGATGGCAAAGACATTCGACGTCTGAACTTGAAGTCTCTTAGGCTGAAAATTGGTTTGGTGCAACAAGAACCAGCTCTCTTTGCAGCAAGCATTTTCGATAACATTGCCTATGGAAAAGAAGGTGCAACCGAAGCAGAAGTAGTCGAAGCTGCACGTACAGCCAATGTGCATGGATTCGTTAGTGGATTGCCTGATGGGTACAAAACTCCTGTTGGGGAGAGAGGGGTTCAGCTCTCAGGAGGACAGAAACAAAGAATTGCTATAGCAAGAGCTGTGCTCAAGGACCCAACAATACTTTTGCTGGATGAGGCCACAAGTGCGCTGGATGCAGAATCAGAATGTGTACTACAAGAAGCACTGGAGAGGCTCATGAGGGGCCGAACCACTGTGCTGGTTGCCCACCGTTTATCAACAATTAGAGGGGTGGATAGCATCGGTGTTGTGCAAGATGGGCGCATTGTGGAACATGGAAGCCACTCAGAACTTGTGAGCCGCCCAGATGGGGCATATTCGAGACTCTTGCAGCTACAAAATCATCGGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010218 GO:0010315 GO:0010329 GO:0010540 GO:0010541 GO:0010646 GO:0010817 GO:0010928 GO:0015562 GO:0016020 GO:0016043 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044464 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048527 GO:0048528 GO:0048583 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048859 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0090691 GO:0090696 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1279

Amino Acids

139.56

Weight (kDa)

8.49

Isoelectric Point (pI)

35.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	44 - 312	3.3e-55	ABC transporter transmembrane region
ABC_tran	PF00005	382 - 558	6.4e-27	ABC transporter
ABC_membrane	PF00664	714 - 984	1.1e-49	ABC transporter transmembrane region
ABC_tran	PF00005	1055 - 1203	2.9e-30	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0009778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28860
fragaria_vesca	FvH4_6g48960 FvH4_6g48960 FvH4_6g48960 FvH4_6g48960 FvH4_6g48960
malus_domestica	MD09G1049400.v1.1 MD17G1049500.v1.1 MD17G1049600.v1.1
prunus_persica	Prupe.3G269400_v2.0.a1 Prupe.3G269400_v2.0.a1
pyrus_communis	pycom111g04040 pycom17g04450
rosa_chinensis	RchiOBHm_Chr2g0168761
rosa_laevigata	RLG00000021808
rosa_multiflora	Rmu_ssc0000393.1_g000001
rosa_roxburghii	Rroxscaffold_2G00082690
rosa_rugosa	Rorug02G0535300
rosa_samantha	Rh2BG619000 Rh2DG629700
rosa_wichuraiana	Rw2G050250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3313
Acc36I	ACCTGC	1 cut(s)	1336
AccB1I	GGYRCC	2 cut(s)	2147, 2882
AccB7I	CCANNNNNTGG	2 cut(s)	1888, 3275
AccIII	TCCGGA	1 cut(s)	1908
AciI	CCGC	6 cut(s)	234, 518, 600, 1194, 2484, 3784
AclWI	GGATC	5 cut(s)	1241, 1642, 2164, 2743, 3260
AcoI	YGGCCR	3 cut(s)	792, 2457, 3190
AcsI	RAATTY	2 cut(s)	736, 1147
AcuI	CTGAAG	3 cut(s)	415, 2317, 2880
AcyI	GRCGYC	1 cut(s)	3310
AdeI	CACNNNGTG	1 cut(s)	2893
AflII	CTTAAG	1 cut(s)	1993
AjiI	CACGTC	1 cut(s)	388
AjnI	CCWGG	5 cut(s)	1121, 1254, 1410, 1419, 2943
AjuI	GAANNNNNNNTTGG	2 cut(s)	3329, 3361
Alw21I	GWGCWC	3 cut(s)	347, 2083, 3570
AlwI	GGATC	5 cut(s)	1241, 1642, 2164, 2743, 3260
AlwNI	CAGNNNCTG	2 cut(s)	1511, 2468
Ama87I	CYCGRG	3 cut(s)	580, 2076, 2434
Aor13HI	TCCGGA	1 cut(s)	1908
ApoI	RAATTY	2 cut(s)	736, 1147
ArsI	GACNNNNNNTTYG	2 cut(s)	2803, 2835
AseI	ATTAAT	1 cut(s)	1871
Asp700I	GAANNNNTTC	2 cut(s)	905, 3093
AspLEI	GCGC	4 cut(s)	559, 1676, 3613, 3747
AspS9I	GGNCC	9 cut(s)	457, 730, 972, 1045, 1408, 2180, 2941, 3574, 3667
AsuC2I	CCSGG	4 cut(s)	581, 582, 2170, 3062
AsuHPI	GGTGA	5 cut(s)	194, 431, 731, 1114, 3020
AsuII	TTCGAA	1 cut(s)	2716
AsuNHI	GCTAGC	1 cut(s)	2380
AvaI	CYCGRG	3 cut(s)	580, 2076, 2434
AvaII	GGWCC	5 cut(s)	457, 1045, 2180, 2941, 3574
AxyI	CCTNAGG	1 cut(s)	3072
BaeGI	GKGCMC	1 cut(s)	2887
BalI	TGGCCA	3 cut(s)	794, 2459, 3192
BanI	GGYRCC	2 cut(s)	2147, 2882
BanII	GRGCYC	4 cut(s)	630, 847, 2083, 2295
BbsI	GAAGAC	5 cut(s)	32, 421, 2261, 2263, 2432
Bbv12I	GWGCWC	3 cut(s)	347, 2083, 3570
BbvCI	CCTCAGC	1 cut(s)	3078
BceAI	ACGGC	4 cut(s)	52, 163, 356, 1169
BcgI	CGANNNNNNTGC	8 cut(s)	13, 47, 389, 423, 3377, 3411, 3793, 3827
BciT130I	CCWGG	5 cut(s)	1123, 1256, 1412, 1421, 2945
BciVI	GTATCC	2 cut(s)	992, 2789
BclI	TGATCA	1 cut(s)	1399
BcnI	CCSGG	4 cut(s)	581, 582, 2170, 3062
BfmI	CTRYAG	7 cut(s)	221, 1503, 1512, 1743, 2301, 2513, 3552
BfoI	RGCGCY	2 cut(s)	560, 1677
BfrI	CTTAAG	1 cut(s)	1993
BfuAI	ACCTGC	1 cut(s)	1336
BfuI	GTATCC	2 cut(s)	992, 2789
BglI	GCCNNNNNGGC	1 cut(s)	791
BlpI	GCTNAGC	3 cut(s)	675, 1336, 2625
BmcAI	AGTACT	2 cut(s)	2020, 2163
Bme1390I	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1412, 1421, 2170, 2945, 3062
Bme18I	GGWCC	5 cut(s)	457, 1045, 2180, 2941, 3574
BmeT110I	CYCGRG	3 cut(s)	580, 2076, 2434
BmgBI	CACGTC	1 cut(s)	388
BmgT120I	GGNCC	9 cut(s)	457, 730, 972, 1045, 1408, 2180, 2941, 3574, 3667
BmrFI	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1412, 1421, 2170, 2945, 3062
BmsI	GCATC	9 cut(s)	397, 454, 739, 1468, 1672, 2367, 2455, 3607, 3732
BmtI	GCTAGC	1 cut(s)	2384
BpiI	GAAGAC	5 cut(s)	32, 421, 2261, 2263, 2432
BpmI	CTGGAG	2 cut(s)	1614, 3671
Bpu10I	CCTNAGC	2 cut(s)	2003, 3078
Bpu1102I	GCTNAGC	3 cut(s)	675, 1336, 2625
Bpu14I	TTCGAA	1 cut(s)	2716
BpuEI	CTTGAG	3 cut(s)	1079, 1472, 3554
BpuMI	CCSGG	4 cut(s)	581, 582, 2170, 3062
BsaAI	YACGTR	1 cut(s)	3446
BsaBI	GATNNNNATC	1 cut(s)	3171
BsaHI	GRCGYC	1 cut(s)	3310
BsaI	GGTCTC	3 cut(s)	124, 2578, 2825
BsaJI	CCNNGG	6 cut(s)	580, 581, 1255, 2084, 2244, 2664
BsaWI	WCCGGW	3 cut(s)	326, 1908, 1980
BsaXI	ACNNNNNCTCC	2 cut(s)	2065, 2095
Bse118I	RCCGGY	2 cut(s)	863, 1285
Bse1I	ACTGG	7 cut(s)	528, 1048, 2011, 2026, 2460, 3193, 3654
Bse21I	CCTNAGG	1 cut(s)	3072
Bse3DI	GCAATG	4 cut(s)	29, 684, 1788, 3393
Bse8I	GATNNNNATC	1 cut(s)	3171
BseAI	TCCGGA	1 cut(s)	1908
BseBI	CCWGG	5 cut(s)	1123, 1256, 1412, 1421, 2945
BseDI	CCNNGG	6 cut(s)	580, 581, 1255, 2084, 2244, 2664
BseJI	GATNNNNATC	1 cut(s)	3171
BseMI	GCAATG	4 cut(s)	29, 684, 1788, 3393
BseNI	ACTGG	7 cut(s)	528, 1048, 2011, 2026, 2460, 3193, 3654
BseRI	GAGGAG	2 cut(s)	1593, 2438
BseSI	GKGCMC	1 cut(s)	2887
BseYI	CCCAGC	2 cut(s)	1165, 2288
BsgI	GTGCAG	2 cut(s)	1413, 3426
BshNI	GGYRCC	2 cut(s)	2147, 2882
BsiHKAI	GWGCWC	3 cut(s)	347, 2083, 3570
BsiHKCI	CYCGRG	3 cut(s)	580, 2076, 2434
BslFI	GGGAC	1 cut(s)	534
BsmBI	CGTCTC	3 cut(s)	442, 1727, 1814
BsmFI	GGGAC	1 cut(s)	534
BsmI	GAATGC	1 cut(s)	905
Bso31I	GGTCTC	3 cut(s)	124, 2578, 2825
BsoBI	CYCGRG	3 cut(s)	580, 2076, 2434
Bsp119I	TTCGAA	1 cut(s)	2716
Bsp1286I	GDGCHC	7 cut(s)	347, 630, 847, 2083, 2295, 2887, 3570
Bsp13I	TCCGGA	1 cut(s)	1908
Bsp1407I	TGTACA	2 cut(s)	321, 1702
Bsp1720I	GCTNAGC	3 cut(s)	675, 1336, 2625
Bsp19I	CCATGG	1 cut(s)	2244
BspACI	CCGC	6 cut(s)	234, 518, 600, 1194, 2484, 3784
BspEI	TCCGGA	1 cut(s)	1908
BspHI	TCATGA	2 cut(s)	1831, 3660
BspMAI	CTGCAG	4 cut(s)	225, 1507, 1516, 2517
BspMI	ACCTGC	1 cut(s)	1336
BspOI	GCTAGC	1 cut(s)	2384
BspPI	GGATC	5 cut(s)	1241, 1642, 2164, 2743, 3260
BspQI	GCTCTTC	2 cut(s)	86, 157
BspT104I	TTCGAA	1 cut(s)	2716
BspT107I	GGYRCC	2 cut(s)	2147, 2882
BspTI	CTTAAG	1 cut(s)	1993
BspTNI	GGTCTC	3 cut(s)	124, 2578, 2825
BsrDI	GCAATG	4 cut(s)	29, 684, 1788, 3393
BsrFI	RCCGGY	2 cut(s)	863, 1285
BsrGI	TGTACA	2 cut(s)	321, 1702
BsrI	ACTGG	7 cut(s)	528, 1048, 2011, 2026, 2460, 3193, 3654
BssAI	RCCGGY	2 cut(s)	863, 1285
BssECI	CCNNGG	6 cut(s)	580, 581, 1255, 2084, 2244, 2664
BssNI	GRCGYC	1 cut(s)	3310
BssT1I	CCWWGG	3 cut(s)	2084, 2244, 2664
Bst2UI	CCWGG	5 cut(s)	1123, 1256, 1412, 1421, 2945
Bst6I	CTCTTC	6 cut(s)	86, 157, 2415, 2607, 2765, 3224
BstACI	GRCGYC	1 cut(s)	3310
BstAFI	CTTAAG	1 cut(s)	1993
BstAPI	GCANNNNNTGC	4 cut(s)	1351, 1436, 1511, 1520
BstAUI	TGTACA	2 cut(s)	321, 1702
BstBAI	YACGTR	1 cut(s)	3446
BstBI	TTCGAA	1 cut(s)	2716
BstDSI	CCRYGG	1 cut(s)	2244
BstH2I	RGCGCY	2 cut(s)	560, 1677
BstHHI	GCGC	4 cut(s)	559, 1676, 3613, 3747
BstNI	CCWGG	5 cut(s)	1123, 1256, 1412, 1421, 2945
BstNSI	RCATGY	2 cut(s)	315, 3122
BstSCI	CCNGG	9 cut(s)	579, 580, 1121, 1254, 1410, 1419, 2168, 2943, 3060
BstSFI	CTRYAG	7 cut(s)	221, 1503, 1512, 1743, 2301, 2513, 3552
BstSLI	GKGCMC	1 cut(s)	2887
BstV2I	GAAGAC	5 cut(s)	32, 421, 2261, 2263, 2432
BstX2I	RGATCY	2 cut(s)	1647, 2748
BstXI	CCANNNNNNTGG	5 cut(s)	802, 1055, 1592, 2816, 3682
BstYI	RGATCY	2 cut(s)	1647, 2748
Bsu36I	CCTNAGG	1 cut(s)	3072
BsuI	GTATCC	2 cut(s)	992, 2789
BtgI	CCRYGG	1 cut(s)	2244
BtgZI	GCGATG	1 cut(s)	1742
BtrI	CACGTC	1 cut(s)	388
BtsI	GCAGTG	4 cut(s)	1204, 1376, 2528, 2658
BtsIMutI	CAGTG	6 cut(s)	1204, 1376, 2528, 2658, 3647, 3675
BveI	ACCTGC	1 cut(s)	1336
CaiI	CAGNNNCTG	2 cut(s)	1511, 2468
CciI	TCATGA	2 cut(s)	1831, 3660
CfoI	GCGC	4 cut(s)	559, 1676, 3613, 3747
Cfr10I	RCCGGY	2 cut(s)	863, 1285
Cfr13I	GGNCC	9 cut(s)	457, 730, 972, 1045, 1408, 2180, 2941, 3574, 3667
Cfr9I	CCCGGG	1 cut(s)	580
CseI	GACGC	1 cut(s)	424
DraI	TTTAAA	1 cut(s)	3163
DraIII	CACNNNGTG	1 cut(s)	2893
EaeI	YGGCCR	3 cut(s)	792, 2457, 3190
Eam1104I	CTCTTC	6 cut(s)	86, 157, 2415, 2607, 2765, 3224
EarI	CTCTTC	6 cut(s)	86, 157, 2415, 2607, 2765, 3224
Ecl136II	GAGCTC	1 cut(s)	2081
Eco130I	CCWWGG	3 cut(s)	2084, 2244, 2664
Eco24I	GRGCYC	4 cut(s)	630, 847, 2083, 2295
Eco31I	GGTCTC	3 cut(s)	124, 2578, 2825
Eco47I	GGWCC	5 cut(s)	457, 1045, 2180, 2941, 3574
Eco53kI	GAGCTC	1 cut(s)	2081
Eco57I	CTGAAG	3 cut(s)	415, 2317, 2880
Eco81I	CCTNAGG	1 cut(s)	3072
Eco88I	CYCGRG	3 cut(s)	580, 2076, 2434
EcoICRI	GAGCTC	1 cut(s)	2081
EcoO109I	RGGNCCY	3 cut(s)	730, 972, 3574
EcoRI	GAATTC	1 cut(s)	736
EcoRII	CCWGG	5 cut(s)	1121, 1254, 1410, 1419, 2943
EcoT14I	CCWWGG	3 cut(s)	2084, 2244, 2664
EcoT38I	GRGCYC	4 cut(s)	630, 847, 2083, 2295
ErhI	CCWWGG	3 cut(s)	2084, 2244, 2664
Esp3I	CGTCTC	3 cut(s)	442, 1727, 1814
FalI	AAGNNNNNCTT	2 cut(s)	360, 392
FaqI	GGGAC	1 cut(s)	534
FauI	CCCGC	2 cut(s)	511, 593
FauNDI	CATATG	1 cut(s)	712
FbaI	TGATCA	1 cut(s)	1399
FriOI	GRGCYC	4 cut(s)	630, 847, 2083, 2295
GlaI	GCGC	4 cut(s)	558, 1675, 3612, 3746
GsaI	CCCAGC	2 cut(s)	1169, 2292
GsuI	CTGGAG	2 cut(s)	1614, 3671
HaeII	RGCGCY	2 cut(s)	560, 1677
HgaI	GACGC	1 cut(s)	424
HhaI	GCGC	4 cut(s)	559, 1676, 3613, 3747
Hin1I	GRCGYC	1 cut(s)	3310
Hin6I	GCGC	4 cut(s)	557, 1674, 3611, 3745
HinP1I	GCGC	4 cut(s)	557, 1674, 3611, 3745
HincII	GTYRAC	2 cut(s)	1783, 1962
HindII	GTYRAC	2 cut(s)	1783, 1962
HphI	GGTGA	5 cut(s)	194, 431, 731, 1114, 3020
Hpy166II	GTNNAC	8 cut(s)	324, 816, 1366, 1417, 1783, 1931, 1962, 3635
Hpy8I	GTNNAC	8 cut(s)	324, 816, 1366, 1417, 1783, 1931, 1962, 3635
Hpy99I	CGWCG	3 cut(s)	445, 2805, 3312
HpyCH4IV	ACGT	3 cut(s)	387, 3310, 3445
HpySE526I	ACGT	3 cut(s)	387, 3310, 3445
Hsp92I	GRCGYC	1 cut(s)	3310
HspAI	GCGC	4 cut(s)	557, 1674, 3611, 3745
Kpn2I	TCCGGA	1 cut(s)	1908
Ksp22I	TGATCA	1 cut(s)	1399
LguI	GCTCTTC	2 cut(s)	86, 157
LmnI	GCTCC	8 cut(s)	134, 1423, 1659, 2086, 2125, 2285, 2805, 3212
LweI	GCATC	9 cut(s)	397, 454, 739, 1468, 1672, 2367, 2455, 3607, 3732
MaeII	ACGT	3 cut(s)	387, 3310, 3445
MaeIII	GTNAC	4 cut(s)	419, 776, 1102, 2950
MfeI	CAATTG	2 cut(s)	1617, 2487
MflI	RGATCY	2 cut(s)	1647, 2748
MhlI	GDGCHC	7 cut(s)	347, 630, 847, 2083, 2295, 2887, 3570
MlsI	TGGCCA	3 cut(s)	794, 2459, 3192
MluNI	TGGCCA	3 cut(s)	794, 2459, 3192
MlyI	GAGTC	3 cut(s)	1590, 2446, 3801
MmeI	TCCRAC	8 cut(s)	1032, 1549, 1609, 1873, 2207, 2386, 2783, 3190
Mox20I	TGGCCA	3 cut(s)	794, 2459, 3192
MroI	TCCGGA	1 cut(s)	1908
MroXI	GAANNNNTTC	2 cut(s)	905, 3093
MscI	TGGCCA	3 cut(s)	794, 2459, 3192
MseI	TTAA	9 cut(s)	192, 378, 705, 1077, 1871, 1994, 2640, 3162, 3171
Msp20I	TGGCCA	3 cut(s)	794, 2459, 3192
MspA1I	CMGCKG	2 cut(s)	995, 1169
MspCI	CTTAAG	1 cut(s)	1993
MspR9I	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1412, 1421, 2170, 2945, 3062
MunI	CAATTG	2 cut(s)	1617, 2487
Mva1269I	GAATGC	1 cut(s)	905
MvaI	CCWGG	5 cut(s)	1123, 1256, 1412, 1421, 2945
NciI	CCSGG	4 cut(s)	581, 582, 2170, 3062
NcoI	CCATGG	1 cut(s)	2244
NdeI	CATATG	1 cut(s)	712
NheI	GCTAGC	1 cut(s)	2380
NmeAIII	GCCGAG	3 cut(s)	252, 328, 668
NmuCI	GTSAC	2 cut(s)	419, 1102
NspI	RCATGY	2 cut(s)	315, 3122
NspV	TTCGAA	1 cut(s)	2716
PaeI	GCATGC	1 cut(s)	315
PaeR7I	CTCGAG	2 cut(s)	2076, 2434
PagI	TCATGA	2 cut(s)	1831, 3660
PciSI	GCTCTTC	2 cut(s)	86, 157
PcsI	WCGNNNNNNNCGW	3 cut(s)	279, 1930, 1939
PctI	GAATGC	1 cut(s)	905
PdmI	GAANNNNTTC	2 cut(s)	905, 3093
PfeI	GAWTC	7 cut(s)	217, 647, 1912, 2509, 3180, 3465, 3623
PflFI	GACNNNGTC	1 cut(s)	425
PflMI	CCANNNNNTGG	2 cut(s)	1888, 3275
PleI	GAGTC	3 cut(s)	1589, 2445, 3801
PpsI	GAGTC	3 cut(s)	1589, 2445, 3801
Ppu21I	YACGTR	1 cut(s)	3446
PpuMI	RGGWCCY	1 cut(s)	3574
PshBI	ATTAAT	1 cut(s)	1871
Psp124BI	GAGCTC	1 cut(s)	2083
Psp5II	RGGWCCY	1 cut(s)	3574
Psp6I	CCWGG	5 cut(s)	1121, 1254, 1410, 1419, 2943
PspFI	CCCAGC	2 cut(s)	1165, 2288
PspGI	CCWGG	5 cut(s)	1121, 1254, 1410, 1419, 2943
PspPI	GGNCC	9 cut(s)	457, 730, 972, 1045, 1408, 2180, 2941, 3574, 3667
PspPPI	RGGWCCY	1 cut(s)	3574
PspXI	VCTCGAGB	2 cut(s)	2076, 2434
PsrI	GAACNNNNNNTAC	2 cut(s)	1991, 2023
PstI	CTGCAG	4 cut(s)	225, 1507, 1516, 2517
PstNI	CAGNNNCTG	2 cut(s)	1511, 2468
PsuI	RGATCY	2 cut(s)	1647, 2748
PsyI	GACNNNGTC	1 cut(s)	425
PvuII	CAGCTG	2 cut(s)	995, 1169
SacI	GAGCTC	1 cut(s)	2083
SapI	GCTCTTC	2 cut(s)	86, 157
SaqAI	TTAA	9 cut(s)	192, 378, 705, 1077, 1871, 1994, 2640, 3162, 3171
Sau96I	GGNCC	9 cut(s)	457, 730, 972, 1045, 1408, 2180, 2941, 3574, 3667
ScaI	AGTACT	2 cut(s)	2020, 2163
SchI	GAGTC	3 cut(s)	1590, 2446, 3801
ScrFI	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1412, 1421, 2170, 2945, 3062
SduI	GDGCHC	7 cut(s)	347, 630, 847, 2083, 2295, 2887, 3570
SfaNI	GCATC	9 cut(s)	397, 454, 739, 1468, 1672, 2367, 2455, 3607, 3732
SfcI	CTRYAG	7 cut(s)	221, 1503, 1512, 1743, 2301, 2513, 3552
SfiI	GGCCNNNNNGGCC	1 cut(s)	791
Sfr274I	CTCGAG	2 cut(s)	2076, 2434
SfuI	TTCGAA	1 cut(s)	2716
SinI	GGWCC	5 cut(s)	457, 1045, 2180, 2941, 3574
SlaI	CTCGAG	2 cut(s)	2076, 2434
SmaI	CCCGGG	1 cut(s)	582
SmlI	CTYRAG	6 cut(s)	1094, 1451, 1993, 2076, 2434, 3569
SmoI	CTYRAG	6 cut(s)	1094, 1451, 1993, 2076, 2434, 3569
SphI	GCATGC	1 cut(s)	315
SsiI	CCGC	6 cut(s)	234, 518, 600, 1194, 2484, 3784
SspI	AATATT	1 cut(s)	1323
SstI	GAGCTC	1 cut(s)	2083
StyD4I	CCNGG	9 cut(s)	579, 580, 1121, 1254, 1410, 1419, 2168, 2943, 3060
StyI	CCWWGG	3 cut(s)	2084, 2244, 2664
TaiI	ACGT	3 cut(s)	390, 3313, 3448
TaqII	GACCGA	1 cut(s)	2168
TatI	WGTACW	6 cut(s)	321, 1702, 2013, 2018, 2161, 3634
TauI	GCSGC	2 cut(s)	1197, 3786
TfiI	GAWTC	7 cut(s)	217, 647, 1912, 2509, 3180, 3465, 3623
Tru1I	TTAA	9 cut(s)	192, 378, 705, 1077, 1871, 1994, 2640, 3162, 3171
Tru9I	TTAA	9 cut(s)	192, 378, 705, 1077, 1871, 1994, 2640, 3162, 3171
TscAI	CASTG	6 cut(s)	1204, 1383, 2535, 2665, 3654, 3682
TseFI	GTSAC	2 cut(s)	419, 1102
Tsp45I	GTSAC	2 cut(s)	419, 1102
TspGWI	ACGGA	3 cut(s)	458, 3173, 3226
TspMI	CCCGGG	1 cut(s)	580
TspRI	CASTG	6 cut(s)	1204, 1383, 2535, 2665, 3654, 3682
Tth111I	GACNNNGTC	1 cut(s)	425
Van91I	CCANNNNNTGG	2 cut(s)	1888, 3275
Vha464I	CTTAAG	1 cut(s)	1993
VpaK11BI	GGWCC	5 cut(s)	457, 1045, 2180, 2941, 3574
VspI	ATTAAT	1 cut(s)	1871
XapI	RAATTY	2 cut(s)	736, 1147
XbaI	TCTAGA	1 cut(s)	1714
XceI	RCATGY	2 cut(s)	315, 3122
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1889, 2018, 3206, 3610
XhoI	CTCGAG	2 cut(s)	2076, 2434
XmaI	CCCGGG	1 cut(s)	580
XmnI	GAANNNNTTC	2 cut(s)	905, 3093
ZraI	GACGTC	1 cut(s)	3311
ZrmI	AGTACT	2 cut(s)	2020, 2163

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.