Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009778

Physical Location & Seq

Genomic Coordinates Forward (+)

77873269 .. 77879700

Gene Structure 6432 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G050250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3753 bp

ATGGCTGACACCACAGAGCCAACCAAGACATTGCCGGAGGCAGAGAAAAAGAAAGAACAGAGCTTGCCGTTTTACCAGCTCTTCTCCTTTGCTGACAAGTATGATTGCCTTCTCATGGTCTCTGGCAGCATAGGAGCCATAATTCACGGCTCTTCTATGCCCGTGTTCTTCCTCTTATTTGGTGAAATGGTTAATGGGTTTGGCAAAAACCAAATGAATCTGCAGAAAATGACCGCCGAAGTAGCCAAGTATGCTCTGTATTTTGTGTATCTCGGCCTGATCGTCTGCTTCTCATCCTACGCCGAGATTGCATGCTGGATGTACACCGGAGAAAGACAAGTGAGCACTCTGAGGAAGAAGTATCTGGAAGCCGTGCTTAAACAAGACGTGGGGTTTTTCGACACGGATGCCAGAACAGGTGACATAGTCTTCAGCGTCTCGACGGACACCCTTCTGGTCCAAGATGCCATTAGCGAGAAGGTGGGGAACTTCATACACTATCTCTCAACATTTCTGGCGGGACTGGTGGTTGGGTTTGTATCAGCATGGAGGCTGGCGCTGCTGAGTGTGGCAGTCATCCCGGGGATTGCTTTTGCTGGCGGGTTGTATGCTTATACGCTTACAGGGCTCACATCCAAGAGCCGAGAATCGTATGCAAATGCGGGCATTATGGCTGAGCAAGCCATTGCACAAGTACGGACAGTTAAATCATATGTTGGTGAAAGCAAGGCCCTGAATTCGTATTCAGATGCCATACAGAACACCTTGCAGCTTGGTTACAAGGCCGGAATGGCCAAAGGTCTGGGGTTAGGGTGTACCTATGGCATAGCTTGTATGTCGTGGGCTCTTGTTTTCTGGTATGCCGGTGTGTTTATCCGAAATGGACAGACTGATGGAGGGAAAGCATTCACAGCCATTTTTTCAGCTATTGTTGGTGGCATGAGCTTGGGTCAGTCATTTTCCAATCTTGGGGCCTTTAGCAAAGGTAAATCAGCTGGTTACAAGTTGATGGAGATTATTAGGCAAAAGCCCACCATAATTCAGGACCAGTTGGATGGAAAGTGCTTGTCTGAGGTTAATGGAAACATAGAACTCAAGGAGGTGACATTCAGCTACCCATCCAGGCCAGATGTTATCATTTTCCGAAATTTTTCAATCTTCTTCCCAGCTGGAAAGACTGTTGCCGTTGTTGGCGGCAGTGGTTCAGGGAAAAGCACCGTTGTCTCTCTGATTGAAAGGTTTTACGATCCTAACCAGGGTCAGGTATTGCTGGATGGTGTGGACATAAGAACACTGCAGCTGAAATGGTTGCGTGATCAGATGGGCCTGGTAAACCAGGAACCTGCACTCTTTGCTACTACCATTCTTGAGAACATACTCTATGGAAAGCCTGATGCAGCAATGGATGAAGTAGAAGCTGCAGCCTCTGCAGCTAATGCTCATAGTTTCATCACTTTGCTTCCTAATGGGTATAACACTCAGGTAGGAGAGCGAGGAGTCCAACTCTCTGGAGGTCAAAAACAGAGAATTGCAATTGCTAGAGCTATGTTGAAAGACCCAAAGATCCTGCTTCTTGATGAAGCAACCAGCGCCCTTGATGCAAGCTCTGAGAGCATTGTTCAAGAAGCTCTAGATCGTCTCATGGTTGGGAGGACAACTGTAGTTGTGGCACATCGCCTTTCCACCATAAGGAATGTTGACAGCATTGCAGTTATACAACAAGGGCAAGTTGTTGAGACAGGAACTCATGAAGAACTGATTGCCAAAGCTGGGGCTTATTCTTCATTAATTCGGTTCCAAGAAATGGTTGGAAACAGAGACTTCAGGAATCCATCTACTCGTTGTTCACGATCATCACGACTAAGCCACTCATTGTCAACCAAGTCTTTGAGCCTCCGGTCTGGCAGCTTAAGGAACCTGAGCTACCAGTACAGTACTGGTGCTGATGGGAGGATAGAGATGATTTCAAATGCTGAGACAGACAGGAAAACTCGAGCTCCAAGGGGATATTTCTTTCGGCTTCTCACGCTAAATGCTCCTGAATGGCCCTATTCAATCATGGGTGCCATAGGATCAGTACTATCCGGGTTTATCGGTCCAACCTTTGCTATTGTAATGAGCAACATGATTGAGGTGTTCTATTATAGAAATCCTGCTTCCATGGAGAGGAAGACAAAGGAGTATGTCTTCATTTACATAGGAGCTGGGCTCTATGCTGTAGTTGCATATTTGATACAACATTACTTCTTCAGCATAATGGGAGAGAACCTCACTACTAGAGTAAGGAGGATGATGCTTGCAGCAATTTTAAGGAACGAAGTCGGATGGTATGATGAAGAGGAGAACAACTCGAGTCTTCTAGCCTCAAAACTGGCCACAGATGCTGCTGATGTGAAATCCGCAATTGCTGAGAGGATTTCTGTGATTCTGCAGAACATGACTTCACTGCTCACTTCATTCATAGTTGCTTTCATTGTGGAATGGAGGGTCTCCCTGCTTATCCTAGCAACCTTTCCTCTTTTGGTTCTTGCCAACTTTGCTCAGCAACTTTCGCTTAAAGGTTTTGCCGGAGACACTGCCAAGGCTCATGCAAAGACGAGCATGATTGCAGGGGAAGGAGTGAGCAACATTCGAACAGTTGCAGCCTTCAATGCACAAGACAAGATCCTCTCTCTCTTCTGCCATGAGCTTCGTATCCCACAACTAGGAAGCCTTCGTCGGAGCCAAACTGCTGGTCTCCTATTTGGCCTCTCACAACTTGCGTTATATGCATCTGAAGCTCTCATTCTATGGTATGGCGCCCACCTTGTGAGCAAAGGTGTCTCAACCTTCTCCAAAGTCATCAAGGTTTTTGTGGTCCTGGTTGTAACAGCCAATTCAGTAGCAGAAACAGTTAGTCTTGCCCCTGAGATTATTAGGGGTGGTGAAGCTGTTGGCTCTGTGTTTTCAATTCTTGATCGCCAAACTAGGATTGACCCGGATGACCCTGAGGCTGAGGTGGTTGAAACAATTCGTGGGGAAATTGAACTTAGGCATGTCGATTTTGCATACCCTTCACGACCTGATATTATGATCTTTAAAGATTTTAATCTCAGAATCCGTACTGGCCAAAGCCAAGCACTTGTTGGAGCTAGTGGTTCGGGGAAGAGTTCTGTGATTGCATTGATCGAGAGGTTCTATGATCCAATAGTTGGGAAGGTAATGATTGATGGCAAAGACATTCGACGTCTGAACTTGAAGTCTCTTAGGCTGAAAATTGGTTTGGTGCAACAAGAACCAGCTCTCTTTGCAGCAAGCATTTTCGATAACATTGCCTATGGAAAAGAAGGTGCAACCGAAGCAGAAGTAGTCGAAGCTGCACGTACAGCCAATGTGCATGGATTTGTTAGTGGATTGCCCGATGGGTACAAAACTCCTGTTGGGGAGAGAGGGGTTCAGCTCTCAGGAGGACAGAAACAAAGAATTGCTATAGCAAGAGCTGTGCTCAAGGACCCAACAATACTTTTGCTGGATGAAGCCACTAGTGCACTGGATGCAGAATCAGAATGTGTACTACAAGAAGCACTGGAGAGGCTCATGAGGGGCCGAACTACTGTGCTGGTTGCCCACCGTTTATCAACAATTAGAGGGGTGGATAGCATCGGTGTGGTGCAAGATGGGCGCATTGTGGAACATGGAAGCCACTCAGAACTTGTGAGCCGCCCAGATGGGGCATATTCGAGACTCTTGCAGCTACAAAATCATCGGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009637 GO:0009639 GO:0009640 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009791 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009966 GO:0009987 GO:0010033 GO:0010218 GO:0010315 GO:0010329 GO:0010540 GO:0010541 GO:0010646 GO:0010817 GO:0010928 GO:0015562 GO:0016020 GO:0016043 GO:0022414 GO:0022622 GO:0022857 GO:0023051 GO:0032501 GO:0032502 GO:0032535 GO:0042221 GO:0043473 GO:0043476 GO:0043478 GO:0043479 GO:0043480 GO:0043481 GO:0044464 GO:0048364 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048527 GO:0048528 GO:0048583 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0048859 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080161 GO:0090066 GO:0090567 GO:0090691 GO:0090696 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1250

Amino Acids

136.37

Weight (kDa)

8.49

Isoelectric Point (pI)

34.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	44 - 312	3.2e-55	ABC transporter transmembrane region
ABC_tran	PF00005	382 - 530	1.6e-31	ABC transporter
ABC_membrane	PF00664	686 - 956	1e-49	ABC transporter transmembrane region
ABC_tran	PF00005	1027 - 1175	2.8e-30	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28860
fragaria_vesca	FvH4_6g48960 FvH4_6g48960 FvH4_6g48960 FvH4_6g48960 FvH4_6g48960
malus_domestica	MD09G1049400.v1.1 MD17G1049500.v1.1 MD17G1049600.v1.1
prunus_persica	Prupe.3G269400_v2.0.a1 Prupe.3G269400_v2.0.a1
pyrus_communis	pycom111g04040 pycom17g04450
rosa_chinensis	RchiOBHm_Chr2g0168761
rosa_laevigata	RLG00000021808
rosa_multiflora	Rmu_ssc0000393.1_g000001
rosa_roxburghii	Rroxscaffold_2G00082690
rosa_rugosa	Rorug02G0535300
rosa_samantha	Rh2BG619000 Rh2DG629700
rosa_wichuraiana	Rw2G050250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3229
Acc36I	ACCTGC	1 cut(s)	1351
AccB1I	GGYRCC	2 cut(s)	2063, 2798
AccB7I	CCANNNNNTGG	2 cut(s)	1804, 3191
AciI	CCGC	7 cut(s)	234, 518, 600, 662, 1194, 2400, 3700
AclWI	GGATC	5 cut(s)	1241, 1558, 2080, 2659, 3176
AcoI	YGGCCR	3 cut(s)	792, 2373, 3106
AcsI	RAATTY	2 cut(s)	736, 1147
AcuI	CTGAAG	4 cut(s)	415, 1807, 2233, 2796
AcyI	GRCGYC	2 cut(s)	2799, 3226
AdeI	CACNNNGTG	1 cut(s)	2809
AflII	CTTAAG	1 cut(s)	1909
AhlI	ACTAGT	1 cut(s)	3521
AjiI	CACGTC	1 cut(s)	388
AjnI	CCWGG	5 cut(s)	1121, 1254, 1326, 1335, 2859
AjuI	GAANNNNNNNTTGG	2 cut(s)	3245, 3277
Alw21I	GWGCWC	4 cut(s)	347, 1999, 3486, 3529
Alw44I	GTGCAC	1 cut(s)	3525
AlwI	GGATC	5 cut(s)	1241, 1558, 2080, 2659, 3176
AlwNI	CAGNNNCTG	2 cut(s)	1427, 2384
Ama87I	CYCGRG	3 cut(s)	580, 1992, 2350
ApaLI	GTGCAC	1 cut(s)	3525
ApoI	RAATTY	2 cut(s)	736, 1147
ArsI	GACNNNNNNTTYG	2 cut(s)	2719, 2751
AseI	ATTAAT	1 cut(s)	1787
Asp700I	GAANNNNTTC	3 cut(s)	905, 1150, 3009
AspLEI	GCGC	4 cut(s)	559, 1592, 2801, 3663
AsuC2I	CCSGG	4 cut(s)	581, 582, 2086, 2978
AsuHPI	GGTGA	5 cut(s)	194, 431, 731, 1114, 2936
AsuII	TTCGAA	1 cut(s)	2632
AvaI	CYCGRG	3 cut(s)	580, 1992, 2350
AvaII	GGWCC	5 cut(s)	457, 1045, 2096, 2857, 3490
AxyI	CCTNAGG	1 cut(s)	2988
BaeGI	GKGCMC	1 cut(s)	3529
BalI	TGGCCA	3 cut(s)	794, 2375, 3108
BanI	GGYRCC	2 cut(s)	2063, 2798
BanII	GRGCYC	4 cut(s)	630, 847, 1999, 2211
BbsI	GAAGAC	4 cut(s)	421, 2177, 2179, 2348
Bbv12I	GWGCWC	4 cut(s)	347, 1999, 3486, 3529
BbvCI	CCTCAGC	1 cut(s)	2994
BceAI	ACGGC	4 cut(s)	52, 163, 356, 1169
BcgI	CGANNNNNNTGC	6 cut(s)	389, 423, 3293, 3327, 3709, 3743
BciT130I	CCWGG	5 cut(s)	1123, 1256, 1328, 1337, 2861
BciVI	GTATCC	1 cut(s)	2705
BclI	TGATCA	1 cut(s)	1315
BcnI	CCSGG	4 cut(s)	581, 582, 2086, 2978
BcuI	ACTAGT	1 cut(s)	3521
BfaI	CTAG	9 cut(s)	1539, 1631, 2277, 2360, 2504, 2705, 2967, 3132, 3522
BfmI	CTRYAG	8 cut(s)	221, 1295, 1419, 1428, 1659, 2217, 2429, 3468
BfoI	RGCGCY	3 cut(s)	560, 1593, 2802
BfrI	CTTAAG	1 cut(s)	1909
BfuAI	ACCTGC	1 cut(s)	1351
BfuI	GTATCC	1 cut(s)	2705
BglI	GCCNNNNNGGC	1 cut(s)	791
BlpI	GCTNAGC	2 cut(s)	675, 2541
BmcAI	AGTACT	2 cut(s)	1936, 2079
Bme1390I	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1328, 1337, 2086, 2861, 2978
Bme18I	GGWCC	5 cut(s)	457, 1045, 2096, 2857, 3490
BmeT110I	CYCGRG	3 cut(s)	580, 1992, 2350
BmgBI	CACGTC	1 cut(s)	388
BmrFI	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1328, 1337, 2086, 2861, 2978
BpiI	GAAGAC	4 cut(s)	421, 2177, 2179, 2348
BpmI	CTGGAG	2 cut(s)	1530, 3587
Bpu10I	CCTNAGC	2 cut(s)	1919, 2994
Bpu1102I	GCTNAGC	2 cut(s)	675, 2541
Bpu14I	TTCGAA	1 cut(s)	2632
BpuEI	CTTGAG	3 cut(s)	1079, 1388, 3470
BpuMI	CCSGG	4 cut(s)	581, 582, 2086, 2978
BsaAI	YACGTR	1 cut(s)	3362
BsaBI	GATNNNNATC	1 cut(s)	3087
BsaHI	GRCGYC	2 cut(s)	2799, 3226
BsaI	GGTCTC	3 cut(s)	124, 2494, 2741
BsaJI	CCNNGG	6 cut(s)	580, 581, 1255, 2000, 2160, 2580
BsaWI	WCCGGW	2 cut(s)	326, 1896
BsaXI	ACNNNNNCTCC	2 cut(s)	1981, 2011
Bse118I	RCCGGY	1 cut(s)	863
Bse1I	ACTGG	8 cut(s)	528, 1048, 1927, 1942, 2376, 3109, 3534, 3570
Bse21I	CCTNAGG	1 cut(s)	2988
Bse3DI	GCAATG	5 cut(s)	29, 684, 1407, 1704, 3309
Bse8I	GATNNNNATC	1 cut(s)	3087
BseBI	CCWGG	5 cut(s)	1123, 1256, 1328, 1337, 2861
BseDI	CCNNGG	6 cut(s)	580, 581, 1255, 2000, 2160, 2580
BseJI	GATNNNNATC	1 cut(s)	3087
BseMI	GCAATG	5 cut(s)	29, 684, 1407, 1704, 3309
BseNI	ACTGG	8 cut(s)	528, 1048, 1927, 1942, 2376, 3109, 3534, 3570
BseRI	GAGGAG	2 cut(s)	1509, 2354
BseSI	GKGCMC	1 cut(s)	3529
BseYI	CCCAGC	3 cut(s)	1165, 1769, 2204
BsgI	GTGCAG	2 cut(s)	1329, 3342
BshNI	GGYRCC	2 cut(s)	2063, 2798
BsiHKAI	GWGCWC	4 cut(s)	347, 1999, 3486, 3529
BsiHKCI	CYCGRG	3 cut(s)	580, 1992, 2350
BsiSI	CCGG	9 cut(s)	35, 327, 581, 786, 864, 1897, 2085, 2568, 2978
BslFI	GGGAC	1 cut(s)	534
BsmBI	CGTCTC	2 cut(s)	442, 1643
BsmFI	GGGAC	1 cut(s)	534
BsmI	GAATGC	1 cut(s)	905
Bso31I	GGTCTC	3 cut(s)	124, 2494, 2741
BsoBI	CYCGRG	3 cut(s)	580, 1992, 2350
Bsp119I	TTCGAA	1 cut(s)	2632
Bsp1286I	GDGCHC	7 cut(s)	347, 630, 847, 1999, 2211, 3486, 3529
Bsp1407I	TGTACA	1 cut(s)	321
Bsp1720I	GCTNAGC	2 cut(s)	675, 2541
Bsp19I	CCATGG	1 cut(s)	2160
BspACI	CCGC	7 cut(s)	234, 518, 600, 662, 1194, 2400, 3700
BspHI	TCATGA	2 cut(s)	1747, 3576
BspMAI	CTGCAG	5 cut(s)	225, 1299, 1423, 1432, 2433
BspMI	ACCTGC	1 cut(s)	1351
BspPI	GGATC	5 cut(s)	1241, 1558, 2080, 2659, 3176
BspQI	GCTCTTC	2 cut(s)	86, 157
BspT104I	TTCGAA	1 cut(s)	2632
BspT107I	GGYRCC	2 cut(s)	2063, 2798
BspTI	CTTAAG	1 cut(s)	1909
BspTNI	GGTCTC	3 cut(s)	124, 2494, 2741
BsrDI	GCAATG	5 cut(s)	29, 684, 1407, 1704, 3309
BsrFI	RCCGGY	1 cut(s)	863
BsrGI	TGTACA	1 cut(s)	321
BsrI	ACTGG	8 cut(s)	528, 1048, 1927, 1942, 2376, 3109, 3534, 3570
BssAI	RCCGGY	1 cut(s)	863
BssECI	CCNNGG	6 cut(s)	580, 581, 1255, 2000, 2160, 2580
BssNI	GRCGYC	2 cut(s)	2799, 3226
BssT1I	CCWWGG	3 cut(s)	2000, 2160, 2580
Bst2UI	CCWGG	5 cut(s)	1123, 1256, 1328, 1337, 2861
Bst4CI	ACNGT	9 cut(s)	703, 1180, 1219, 1660, 1934, 2638, 2893, 3595, 3611
Bst6I	CTCTTC	5 cut(s)	86, 157, 2331, 2681, 3140
BstACI	GRCGYC	2 cut(s)	2799, 3226
BstAFI	CTTAAG	1 cut(s)	1909
BstAPI	GCANNNNNTGC	4 cut(s)	1352, 1427, 1436, 3533
BstAUI	TGTACA	1 cut(s)	321
BstBAI	YACGTR	1 cut(s)	3362
BstBI	TTCGAA	1 cut(s)	2632
BstC8I	GCNNGC	9 cut(s)	65, 313, 555, 598, 664, 681, 1603, 2298, 3295
BstDSI	CCRYGG	1 cut(s)	2160
BstH2I	RGCGCY	3 cut(s)	560, 1593, 2802
BstHHI	GCGC	4 cut(s)	559, 1592, 2801, 3663
BstNI	CCWGG	5 cut(s)	1123, 1256, 1328, 1337, 2861
BstNSI	RCATGY	2 cut(s)	315, 3038
BstSCI	CCNGG	9 cut(s)	579, 580, 1121, 1254, 1326, 1335, 2084, 2859, 2976
BstSFI	CTRYAG	8 cut(s)	221, 1295, 1419, 1428, 1659, 2217, 2429, 3468
BstSLI	GKGCMC	1 cut(s)	3529
BstV2I	GAAGAC	4 cut(s)	421, 2177, 2179, 2348
BstX2I	RGATCY	2 cut(s)	1563, 2664
BstXI	CCANNNNNNTGG	4 cut(s)	802, 1055, 1508, 2732
BstYI	RGATCY	2 cut(s)	1563, 2664
Bsu36I	CCTNAGG	1 cut(s)	2988
BsuI	GTATCC	1 cut(s)	2705
BtgI	CCRYGG	1 cut(s)	2160
BtgZI	GCGATG	1 cut(s)	1658
BtrI	CACGTC	1 cut(s)	388
BtsI	GCAGTG	4 cut(s)	1204, 1292, 2444, 2574
BtsIMutI	CAGTG	6 cut(s)	1204, 1292, 2444, 2574, 3527, 3563
BveI	ACCTGC	1 cut(s)	1351
Cac8I	GCNNGC	9 cut(s)	65, 313, 555, 598, 664, 681, 1603, 2298, 3295
CaiI	CAGNNNCTG	2 cut(s)	1427, 2384
CciI	TCATGA	2 cut(s)	1747, 3576
CfoI	GCGC	4 cut(s)	559, 1592, 2801, 3663
Cfr10I	RCCGGY	1 cut(s)	863
Cfr9I	CCCGGG	1 cut(s)	580
CseI	GACGC	1 cut(s)	424
DinI	GGCGCC	1 cut(s)	2800
DraI	TTTAAA	1 cut(s)	3079
DraIII	CACNNNGTG	1 cut(s)	2809
EaeI	YGGCCR	3 cut(s)	792, 2373, 3106
Eam1104I	CTCTTC	5 cut(s)	86, 157, 2331, 2681, 3140
EarI	CTCTTC	5 cut(s)	86, 157, 2331, 2681, 3140
Ecl136II	GAGCTC	1 cut(s)	1997
Eco130I	CCWWGG	3 cut(s)	2000, 2160, 2580
Eco24I	GRGCYC	4 cut(s)	630, 847, 1999, 2211
Eco31I	GGTCTC	3 cut(s)	124, 2494, 2741
Eco47I	GGWCC	5 cut(s)	457, 1045, 2096, 2857, 3490
Eco53kI	GAGCTC	1 cut(s)	1997
Eco57I	CTGAAG	4 cut(s)	415, 1807, 2233, 2796
Eco81I	CCTNAGG	1 cut(s)	2988
Eco88I	CYCGRG	3 cut(s)	580, 1992, 2350
EcoICRI	GAGCTC	1 cut(s)	1997
EcoO109I	RGGNCCY	3 cut(s)	730, 972, 3490
EcoRI	GAATTC	1 cut(s)	736
EcoRII	CCWGG	5 cut(s)	1121, 1254, 1326, 1335, 2859
EcoT14I	CCWWGG	3 cut(s)	2000, 2160, 2580
EcoT22I	ATGCAT	1 cut(s)	2773
EcoT38I	GRGCYC	4 cut(s)	630, 847, 1999, 2211
EgeI	GGCGCC	1 cut(s)	2800
EheI	GGCGCC	1 cut(s)	2800
ErhI	CCWWGG	3 cut(s)	2000, 2160, 2580
Esp3I	CGTCTC	2 cut(s)	442, 1643
FalI	AAGNNNNNCTT	2 cut(s)	360, 392
FaqI	GGGAC	1 cut(s)	534
FauI	CCCGC	3 cut(s)	511, 593, 655
FauNDI	CATATG	1 cut(s)	712
FbaI	TGATCA	1 cut(s)	1315
FriOI	GRGCYC	4 cut(s)	630, 847, 1999, 2211
FspBI	CTAG	9 cut(s)	1539, 1631, 2277, 2360, 2504, 2705, 2967, 3132, 3522
GlaI	GCGC	4 cut(s)	558, 1591, 2800, 3662
GsaI	CCCAGC	3 cut(s)	1169, 1773, 2208
GsuI	CTGGAG	2 cut(s)	1530, 3587
HaeII	RGCGCY	3 cut(s)	560, 1593, 2802
HapII	CCGG	9 cut(s)	35, 327, 581, 786, 864, 1897, 2085, 2568, 2978
HgaI	GACGC	1 cut(s)	424
HhaI	GCGC	4 cut(s)	559, 1592, 2801, 3663
Hin1I	GRCGYC	2 cut(s)	2799, 3226
Hin6I	GCGC	4 cut(s)	557, 1590, 2799, 3661
HinP1I	GCGC	4 cut(s)	557, 1590, 2799, 3661
HincII	GTYRAC	2 cut(s)	1699, 1878
HindII	GTYRAC	2 cut(s)	1699, 1878
HinfI	GANTC	9 cut(s)	217, 647, 1497, 1828, 2353, 2425, 3096, 3539, 3723
HpaII	CCGG	9 cut(s)	35, 327, 581, 786, 864, 1897, 2085, 2568, 2978
HphI	GGTGA	5 cut(s)	194, 431, 731, 1114, 2936
Hpy166II	GTNNAC	9 cut(s)	324, 816, 1282, 1333, 1699, 1847, 1878, 3527, 3551
Hpy8I	GTNNAC	9 cut(s)	324, 816, 1282, 1333, 1699, 1847, 1878, 3527, 3551
Hpy99I	CGWCG	3 cut(s)	445, 2721, 3228
HpyCH4III	ACNGT	9 cut(s)	703, 1180, 1219, 1660, 1934, 2638, 2893, 3595, 3611
HpyCH4IV	ACGT	3 cut(s)	387, 3226, 3361
HpySE526I	ACGT	3 cut(s)	387, 3226, 3361
Hsp92I	GRCGYC	2 cut(s)	2799, 3226
HspAI	GCGC	4 cut(s)	557, 1590, 2799, 3661
KasI	GGCGCC	1 cut(s)	2798
Ksp22I	TGATCA	1 cut(s)	1315
LguI	GCTCTTC	2 cut(s)	86, 157
LmnI	GCTCC	6 cut(s)	134, 2002, 2041, 2201, 2721, 3128
MaeI	CTAG	9 cut(s)	1539, 1631, 2277, 2360, 2504, 2705, 2967, 3132, 3522
MaeII	ACGT	3 cut(s)	387, 3226, 3361
MaeIII	GTNAC	5 cut(s)	419, 776, 998, 1102, 2866
MfeI	CAATTG	2 cut(s)	1533, 2403
MflI	RGATCY	2 cut(s)	1563, 2664
MhlI	GDGCHC	7 cut(s)	347, 630, 847, 1999, 2211, 3486, 3529
MlsI	TGGCCA	3 cut(s)	794, 2375, 3108
MluNI	TGGCCA	3 cut(s)	794, 2375, 3108
Mly113I	GGCGCC	1 cut(s)	2799
MlyI	GAGTC	3 cut(s)	1506, 2362, 3717
MmeI	TCCRAC	7 cut(s)	1032, 1525, 1789, 2123, 2302, 2699, 3106
Mox20I	TGGCCA	3 cut(s)	794, 2375, 3108
Mph1103I	ATGCAT	1 cut(s)	2773
MroXI	GAANNNNTTC	3 cut(s)	905, 1150, 3009
MscI	TGGCCA	3 cut(s)	794, 2375, 3108
MslI	CAYNNNNRTG	1 cut(s)	3644
Msp20I	TGGCCA	3 cut(s)	794, 2375, 3108
MspA1I	CMGCKG	3 cut(s)	995, 1169, 1300
MspCI	CTTAAG	1 cut(s)	1909
MspI	CCGG	9 cut(s)	35, 327, 581, 786, 864, 1897, 2085, 2568, 2978
MspR9I	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1328, 1337, 2086, 2861, 2978
MunI	CAATTG	2 cut(s)	1533, 2403
Mva1269I	GAATGC	1 cut(s)	905
MvaI	CCWGG	5 cut(s)	1123, 1256, 1328, 1337, 2861
NarI	GGCGCC	1 cut(s)	2799
NciI	CCSGG	4 cut(s)	581, 582, 2086, 2978
NcoI	CCATGG	1 cut(s)	2160
NdeI	CATATG	1 cut(s)	712
NmeAIII	GCCGAG	3 cut(s)	252, 328, 668
NmuCI	GTSAC	2 cut(s)	419, 1102
NsiI	ATGCAT	1 cut(s)	2773
NspI	RCATGY	2 cut(s)	315, 3038
NspV	TTCGAA	1 cut(s)	2632
PaeI	GCATGC	1 cut(s)	315
PaeR7I	CTCGAG	2 cut(s)	1992, 2350
PagI	TCATGA	2 cut(s)	1747, 3576
PciSI	GCTCTTC	2 cut(s)	86, 157
PcsI	WCGNNNNNNNCGW	3 cut(s)	279, 1846, 1855
PctI	GAATGC	1 cut(s)	905
PdmI	GAANNNNTTC	3 cut(s)	905, 1150, 3009
PfeI	GAWTC	6 cut(s)	217, 647, 1828, 2425, 3096, 3539
PflFI	GACNNNGTC	1 cut(s)	425
PflMI	CCANNNNNTGG	2 cut(s)	1804, 3191
PleI	GAGTC	3 cut(s)	1505, 2361, 3717
PluTI	GGCGCC	1 cut(s)	2802
PpsI	GAGTC	3 cut(s)	1505, 2361, 3717
Ppu21I	YACGTR	1 cut(s)	3362
PpuMI	RGGWCCY	1 cut(s)	3490
PshBI	ATTAAT	1 cut(s)	1787
Psp124BI	GAGCTC	1 cut(s)	1999
Psp5II	RGGWCCY	1 cut(s)	3490
Psp6I	CCWGG	5 cut(s)	1121, 1254, 1326, 1335, 2859
PspFI	CCCAGC	3 cut(s)	1165, 1769, 2204
PspGI	CCWGG	5 cut(s)	1121, 1254, 1326, 1335, 2859
PspPPI	RGGWCCY	1 cut(s)	3490
PspXI	VCTCGAGB	2 cut(s)	1992, 2350
PsrI	GAACNNNNNNTAC	2 cut(s)	1907, 1939
PstI	CTGCAG	5 cut(s)	225, 1299, 1423, 1432, 2433
PstNI	CAGNNNCTG	2 cut(s)	1427, 2384
PsuI	RGATCY	2 cut(s)	1563, 2664
PsyI	GACNNNGTC	1 cut(s)	425
PvuII	CAGCTG	3 cut(s)	995, 1169, 1300
RseI	CAYNNNNRTG	1 cut(s)	3644
SacI	GAGCTC	1 cut(s)	1999
SapI	GCTCTTC	2 cut(s)	86, 157
ScaI	AGTACT	2 cut(s)	1936, 2079
SchI	GAGTC	3 cut(s)	1506, 2362, 3717
ScrFI	CCNGG	9 cut(s)	581, 582, 1123, 1256, 1328, 1337, 2086, 2861, 2978
SduI	GDGCHC	7 cut(s)	347, 630, 847, 1999, 2211, 3486, 3529
SfcI	CTRYAG	8 cut(s)	221, 1295, 1419, 1428, 1659, 2217, 2429, 3468
SfiI	GGCCNNNNNGGCC	1 cut(s)	791
SfoI	GGCGCC	1 cut(s)	2800
Sfr274I	CTCGAG	2 cut(s)	1992, 2350
SfuI	TTCGAA	1 cut(s)	2632
SinI	GGWCC	5 cut(s)	457, 1045, 2096, 2857, 3490
SlaI	CTCGAG	2 cut(s)	1992, 2350
SmaI	CCCGGG	1 cut(s)	582
SmiMI	CAYNNNNRTG	1 cut(s)	3644
SmlI	CTYRAG	6 cut(s)	1094, 1367, 1909, 1992, 2350, 3485
SmoI	CTYRAG	6 cut(s)	1094, 1367, 1909, 1992, 2350, 3485
SpeI	ACTAGT	1 cut(s)	3521
SphI	GCATGC	1 cut(s)	315
SsiI	CCGC	7 cut(s)	234, 518, 600, 662, 1194, 2400, 3700
SspDI	GGCGCC	1 cut(s)	2798
SspMI	CTAG	9 cut(s)	1539, 1631, 2277, 2360, 2504, 2705, 2967, 3132, 3522
SstI	GAGCTC	1 cut(s)	1999
StyD4I	CCNGG	9 cut(s)	579, 580, 1121, 1254, 1326, 1335, 2084, 2859, 2976
StyI	CCWWGG	3 cut(s)	2000, 2160, 2580
TaaI	ACNGT	9 cut(s)	703, 1180, 1219, 1660, 1934, 2638, 2893, 3595, 3611
TaiI	ACGT	3 cut(s)	390, 3229, 3364
TaqII	GACCGA	1 cut(s)	2084
TatI	WGTACW	5 cut(s)	321, 1929, 1934, 2077, 3550
TauI	GCSGC	2 cut(s)	1197, 3702
TfiI	GAWTC	6 cut(s)	217, 647, 1828, 2425, 3096, 3539
TscAI	CASTG	6 cut(s)	1204, 1299, 2451, 2581, 3534, 3570
TseFI	GTSAC	2 cut(s)	419, 1102
Tsp45I	GTSAC	2 cut(s)	419, 1102
TspGWI	ACGGA	4 cut(s)	419, 458, 712, 3089
TspMI	CCCGGG	1 cut(s)	580
TspRI	CASTG	6 cut(s)	1204, 1299, 2451, 2581, 3534, 3570
Tth111I	GACNNNGTC	1 cut(s)	425
Van91I	CCANNNNNTGG	2 cut(s)	1804, 3191
Vha464I	CTTAAG	1 cut(s)	1909
VneI	GTGCAC	1 cut(s)	3525
VpaK11BI	GGWCC	5 cut(s)	457, 1045, 2096, 2857, 3490
VspI	ATTAAT	1 cut(s)	1787
XapI	RAATTY	2 cut(s)	736, 1147
XbaI	TCTAGA	1 cut(s)	1630
XceI	RCATGY	2 cut(s)	315, 3038
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1805, 1934, 3122, 3526
XhoI	CTCGAG	2 cut(s)	1992, 2350
XmaI	CCCGGG	1 cut(s)	580
XmnI	GAANNNNTTC	3 cut(s)	905, 1150, 3009
XspI	CTAG	9 cut(s)	1539, 1631, 2277, 2360, 2504, 2705, 2967, 3132, 3522
ZraI	GACGTC	1 cut(s)	3227
ZrmI	AGTACT	2 cut(s)	1936, 2079
Zsp2I	ATGCAT	1 cut(s)	2773

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.