RLG00000023520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018236
Physical Location & Seq
Forward (+)
24823963 .. 24825145
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023520

Sequence Viewer

Length: 450 bp
ATGACTACAACCAGCTCCGGTCCTAATACTAGGGTTGCCTTCCCATGGGCATTCATGCACACTACAGATCGATGTCGGCAACTATATAGTGATAAGGAGCCAAAGAACATTGGTGGAAAGCTTCCTAAAGCTCCGCCAAGGTTGACTCGTCAAGAGTACAGGGTGAAAACCACATCAAACTTACAGGAGCATACTAGTTTAGAAGTGGAGCCAACAATTACCACTAATCATGTAGTTGAGTCGGTGGTTCTAGCAAATGATGTTAATCACGTAATTACGCCAATGGGTGCAGCTTTAAATGGTGTAGTTGTGGGAAATATTTCTCCAAGTGTTGATCATGGGTCACCTCAAGAGCACCAAGTTCTTATTCAAGAGAGTGTTCAGCAGGATTTGGTTGATGAGAGAAGCCCCAATAATGGGAAACTTCAAGTTCAGTACCAGGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.52

Weight (kDa)

6.28

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 134
AfaI GTAC 2 cut(s) 158, 437
AfiI CCNNNNNNNGG 3 cut(s) 45, 416, 417
AgsI TTSAA 2 cut(s) 371, 428
AhlI ACTAGT 1 cut(s) 194
AjnI CCWGG 1 cut(s) 438
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 4 cut(s) 15, 121, 131, 293
AluI AGCT 4 cut(s) 15, 121, 131, 293
Alw21I GWGCWC 1 cut(s) 357
ApeKI GCWGC 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 2 cut(s) 175, 336
AvaII GGWCC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 440
BclI TGATCA 1 cut(s) 334
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 3 cut(s) 30, 195, 251
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 440
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 2 cut(s) 99, 210
BmrFI CCNGG 1 cut(s) 440
BpuEI CTTGAG 1 cut(s) 333
Bsa29I ATCGAT 1 cut(s) 70
BsaAI YACGTR 1 cut(s) 271
BsaBI GATNNNNATC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 44, 137
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 416, 417
Bse8I GATNNNNATC 1 cut(s) 264
BseBI CCWGG 1 cut(s) 440
BseCI ATCGAT 1 cut(s) 70
BseDI CCNNGG 2 cut(s) 44, 137
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 3 cut(s) 45, 416, 417
BseXI GCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 309
BshVI ATCGAT 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 1 cut(s) 18
BslI CCNNNNNNNGG 3 cut(s) 45, 416, 417
BsmI GAATGC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 2 cut(s) 67, 334
Bsp19I CCATGG 1 cut(s) 44
BspACI CCGC 1 cut(s) 134
BspDI ATCGAT 1 cut(s) 70
BspLI GGNNCC 2 cut(s) 99, 210
BssECI CCNNGG 2 cut(s) 44, 137
BssMI GATC 2 cut(s) 67, 334
BssT1I CCWWGG 2 cut(s) 44, 137
Bst2UI CCWGG 1 cut(s) 440
BstBAI YACGTR 1 cut(s) 271
BstDSI CCRYGG 1 cut(s) 44
BstEII GGTNACC 1 cut(s) 342
BstKTI GATC 2 cut(s) 70, 337
BstMBI GATC 2 cut(s) 67, 334
BstNI CCWGG 1 cut(s) 440
BstPI GGTNACC 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 438
BstSFI CTRYAG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 302
Bsu15I ATCGAT 1 cut(s) 70
BsuTUI ATCGAT 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 70
Csp6I GTAC 2 cut(s) 157, 436
CviAII CATG 4 cut(s) 45, 55, 230, 338
CviJI RGCY 7 cut(s) 15, 100, 121, 131, 211, 293, 408
CviKI_1 RGCY 7 cut(s) 15, 100, 121, 131, 211, 293, 408
CviQI GTAC 2 cut(s) 157, 436
DpnI GATC 2 cut(s) 69, 336
DpnII GATC 2 cut(s) 67, 334
DraI TTTAAA 1 cut(s) 297
EciI GGCGGA 1 cut(s) 123
Eco130I CCWWGG 2 cut(s) 44, 137
Eco47I GGWCC 1 cut(s) 20
Eco91I GGTNACC 1 cut(s) 342
EcoO65I GGTNACC 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 44, 137
ErhI CCWWGG 2 cut(s) 44, 137
FaeI CATG 4 cut(s) 48, 58, 233, 341
FaiI YATR 7 cut(s) 46, 56, 85, 87, 192, 231, 339
FatI CATG 4 cut(s) 44, 54, 229, 337
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 3 cut(s) 30, 195, 251
GluI GCNGC 1 cut(s) 291
HapII CCGG 1 cut(s) 18
Hin1II CATG 4 cut(s) 48, 58, 233, 341
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 2 cut(s) 145, 239
HpaII CCGG 1 cut(s) 18
HphI GGTGA 2 cut(s) 175, 336
Hpy166II GTNNAC 1 cut(s) 144
Hpy188III TCNNGA 3 cut(s) 152, 350, 371
Hpy8I GTNNAC 1 cut(s) 144
HpyAV CCTTC 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 2 cut(s) 58, 290
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 4 cut(s) 48, 58, 233, 341
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 67, 334
LmnI GCTCC 5 cut(s) 20, 97, 136, 187, 208
LpnPI CCDG 6 cut(s) 25, 31, 145, 170, 371, 425
Lsp1109I GCAGC 1 cut(s) 302
MaeI CTAG 3 cut(s) 30, 195, 251
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 342
MalI GATC 2 cut(s) 69, 336
MboI GATC 2 cut(s) 67, 334
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 2 cut(s) 216, 273
MlyI GAGTC 2 cut(s) 139, 248
MnlI CCTC 1 cut(s) 357
MroXI GAANNNNTTC 1 cut(s) 319
MseI TTAA 2 cut(s) 264, 296
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 1 cut(s) 440
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 1 cut(s) 440
NcoI CCATGG 1 cut(s) 44
NdeII GATC 2 cut(s) 67, 334
NlaIII CATG 4 cut(s) 48, 58, 233, 341
NlaIV GGNNCC 2 cut(s) 99, 210
NmuCI GTSAC 1 cut(s) 342
PctI GAATGC 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 319
PkrI GCNGC 1 cut(s) 292
PleI GAGTC 2 cut(s) 139, 247
PpsI GAGTC 2 cut(s) 139, 247
Ppu21I YACGTR 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 342
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 99, 210
PspPI GGNCC 1 cut(s) 20
RsaI GTAC 2 cut(s) 158, 437
RsaNI GTAC 2 cut(s) 157, 436
SaqAI TTAA 2 cut(s) 264, 296
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 2 cut(s) 67, 334
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 2 cut(s) 139, 248
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 1 cut(s) 357
SetI ASST 7 cut(s) 17, 123, 133, 143, 273, 295, 349
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
SpeI ACTAGT 1 cut(s) 194
Sse9I AATT 2 cut(s) 216, 273
SsiI CCGC 1 cut(s) 134
SspI AATATT 1 cut(s) 319
SspMI CTAG 3 cut(s) 30, 195, 251
StyD4I CCNGG 1 cut(s) 438
StyI CCWWGG 2 cut(s) 44, 137
TaiI ACGT 1 cut(s) 273
TaqI TCGA 1 cut(s) 70
TasI AATT 2 cut(s) 216, 273
TatI WGTACW 1 cut(s) 156
Tru1I TTAA 2 cut(s) 264, 296
Tru9I TTAA 2 cut(s) 264, 296
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 20
XmnI GAANNNNTTC 1 cut(s) 319
XspI CTAG 3 cut(s) 30, 195, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.