RLG00000032010

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018236
Physical Location & Seq
Forward (+)
10173201 .. 10174915
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032010

Sequence Viewer

Length: 819 bp
ATGAATATTTACTATAAGGCGATAGGCCAAAACAGCTGGAATTGTGACTTCAGAGGATCTGCTCTCATCACTCAGATTGATCCAAATCGTTGTCGACAAATGAACATTGATAAGGAGCGAGGGAACAATGGTGAAAAGTTTGCTAAACCTCAGTCAAGGTTGACTCGTCAAGAGTACAGGGTGAAATCCACATCAAATACACAGGAGCATATTAGTTTAGTAGTTGAGCCAACAATGACTAACCCAATTGAATCAGTGGTTCCAGAAAACGATGTTAATCAAGTAATTACGCCACAAGGTGCAACCTCAATTGGTGTAGTTGTGGGCGCAAATCCTCCAGGAGTTGATCATGGCTCACCTCATGTGTGCCAAGTTCAGCAGGATATGGTTGACACAAATGGAAACCTTCTAGTTCAACACCAAGAGACTGGAGGCCTTTCTCCGGTTCTTCGCGAGAACCAATATGCTATTCTGGCCCAAGAAGCCATTGAGAGCGTGGCTAATGATTTAATTGTTGAAATGGCAGACCATGTGATTAATGAAATAAGGGATAATTCCCTTGAGCTCAGGAAAGGTCCTGAAACTTCAGATATTGGTGCAGTTTCAAAACTAGCCTTGGTTGCAGTTGGCACGGAGTCTCATGCTTCACCAAGTGAGGTCAATGGAGACAAGGCAAATGATGTTGGCATGACATTGGTGGTGTCAAAATCGCAGCAGAAAGCTTTAAAAAAGAAATTGCGTGAGGCGAAAATGAATGCAGAAGCTTCAGTTCGTTACCCTGCACGTAACAGGGCTCCTGCCTCACGCCTTAATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

273

Amino Acids

29.7

Weight (kDa)

6.1

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 94
AccII CGCG 1 cut(s) 453
AclWI GGATC 2 cut(s) 64, 74
AcuI CTGAAG 3 cut(s) 34, 570, 750
AdeI CACNNNGTG 2 cut(s) 299, 653
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 1 cut(s) 442
AgsI TTSAA 4 cut(s) 251, 416, 518, 606
AjnI CCWGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 453, 485
AluBI AGCT 4 cut(s) 36, 565, 722, 764
AluI AGCT 4 cut(s) 36, 565, 722, 764
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 3 cut(s) 419, 642, 660
AlwI GGATC 2 cut(s) 64, 74
AoxI GGCC 3 cut(s) 25, 433, 474
ApeKI GCWGC 1 cut(s) 712
AseI ATTAAT 1 cut(s) 537
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 2 cut(s) 475, 575
AsuHPI GGTGA 4 cut(s) 143, 193, 348, 639
AvaII GGWCC 1 cut(s) 575
BanII GRGCYC 2 cut(s) 567, 796
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 724
BciT130I CCWGG 1 cut(s) 339
BclI TGATCA 1 cut(s) 346
BcoDI GTCTC 3 cut(s) 419, 642, 660
BfaI CTAG 2 cut(s) 410, 611
BisI GCNGC 1 cut(s) 713
BlsI GCNGC 1 cut(s) 714
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 2 cut(s) 475, 575
BmiI GGNNCC 2 cut(s) 261, 795
BmrFI CCNGG 1 cut(s) 339
BpmI CTGGAG 2 cut(s) 321, 450
Bpu10I CCTNAGC 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 581
BsaAI YACGTR 1 cut(s) 785
BsaBI GATNNNNATC 2 cut(s) 84, 276
BsaJI CCNNGG 1 cut(s) 615
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse1I ACTGG 1 cut(s) 433
Bse8I GATNNNNATC 2 cut(s) 84, 276
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 615
BseJI GATNNNNATC 2 cut(s) 84, 276
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMII CTCAG 3 cut(s) 86, 164, 580
BseNI ACTGG 1 cut(s) 433
BseXI GCAGC 1 cut(s) 724
BsgI GTGCAG 2 cut(s) 618, 765
Bsh1236I CGCG 1 cut(s) 453
BshFI GGCC 3 cut(s) 27, 435, 476
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 1 cut(s) 443
BslI CCNNNNNNNGG 1 cut(s) 442
BsmAI GTCTC 3 cut(s) 419, 642, 660
BsmI GAATGC 1 cut(s) 760
BsnI GGCC 3 cut(s) 27, 435, 476
Bsp1286I GDGCHC 2 cut(s) 567, 796
Bsp143I GATC 3 cut(s) 56, 79, 346
Bsp68I TCGCGA 1 cut(s) 453
BspANI GGCC 3 cut(s) 27, 435, 476
BspCNI CTCAG 3 cut(s) 85, 163, 579
BspFNI CGCG 1 cut(s) 453
BspLI GGNNCC 2 cut(s) 261, 795
BspPI GGATC 2 cut(s) 64, 74
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 1 cut(s) 615
BssMI GATC 3 cut(s) 56, 79, 346
BssT1I CCWWGG 1 cut(s) 615
Bst2UI CCWGG 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 785
BstDEI CTNAG 3 cut(s) 72, 150, 566
BstFNI CGCG 1 cut(s) 453
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 3 cut(s) 59, 82, 349
BstMAI GTCTC 3 cut(s) 419, 642, 660
BstMBI GATC 3 cut(s) 56, 79, 346
BstMWI GCNNNNNNNGC 4 cut(s) 33, 473, 482, 620
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 724
BstX2I RGATCY 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 3 cut(s) 27, 435, 476
BtsIMutI CAGTG 1 cut(s) 261
BtuMI TCGCGA 1 cut(s) 453
CfoI GCGC 1 cut(s) 329
Cfr13I GGNCC 2 cut(s) 475, 575
Csp6I GTAC 1 cut(s) 175
CviAII CATG 5 cut(s) 350, 362, 530, 641, 688
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 3 cut(s) 72, 150, 566
DpnI GATC 3 cut(s) 58, 81, 348
DpnII GATC 3 cut(s) 56, 79, 346
DraI TTTAAA 1 cut(s) 726
DraIII CACNNNGTG 2 cut(s) 299, 653
Ecl136II GAGCTC 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 615
Eco147I AGGCCT 1 cut(s) 435
Eco24I GRGCYC 2 cut(s) 567, 796
Eco47I GGWCC 1 cut(s) 575
Eco53kI GAGCTC 1 cut(s) 565
Eco57I CTGAAG 3 cut(s) 34, 570, 750
EcoICRI GAGCTC 1 cut(s) 565
EcoO109I RGGNCCY 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 615
EcoT38I GRGCYC 2 cut(s) 567, 796
ErhI CCWWGG 1 cut(s) 615
FaeI CATG 5 cut(s) 353, 365, 533, 644, 691
FatI CATG 5 cut(s) 349, 361, 529, 640, 687
FbaI TGATCA 1 cut(s) 346
FblI GTMKAC 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 713
FriOI GRGCYC 2 cut(s) 567, 796
Fsp4HI GCNGC 1 cut(s) 713
FspBI CTAG 2 cut(s) 410, 611
GlaI GCGC 1 cut(s) 328
GluI GCNGC 1 cut(s) 713
GsuI CTGGAG 2 cut(s) 321, 450
HaeIII GGCC 3 cut(s) 27, 435, 476
HapII CCGG 1 cut(s) 443
HhaI GCGC 1 cut(s) 329
Hin1II CATG 5 cut(s) 353, 365, 533, 644, 691
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 3 cut(s) 95, 162, 391
HindII GTYRAC 3 cut(s) 95, 162, 391
HindIII AAGCTT 2 cut(s) 720, 762
HinfI GANTC 3 cut(s) 163, 251, 635
HpaII CCGG 1 cut(s) 443
HphI GGTGA 4 cut(s) 143, 193, 348, 639
Hpy166II GTNNAC 3 cut(s) 95, 162, 391
Hpy188I TCNGA 3 cut(s) 53, 75, 589
Hpy188III TCNNGA 5 cut(s) 170, 263, 452, 568, 578
Hpy8I GTNNAC 3 cut(s) 95, 162, 391
HpyAV CCTTC 1 cut(s) 416
HpyCH4IV ACGT 1 cut(s) 784
HpyCH4V TGCA 5 cut(s) 302, 599, 623, 758, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 473, 482, 620
HpyF3I CTNAG 3 cut(s) 72, 150, 566
HpySE526I ACGT 1 cut(s) 784
Hsp92II CATG 5 cut(s) 353, 365, 533, 644, 691
HspAI GCGC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 3 cut(s) 56, 79, 346
LmnI GCTCC 3 cut(s) 115, 205, 799
Lsp1109I GCAGC 1 cut(s) 724
MaeI CTAG 2 cut(s) 410, 611
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 3 cut(s) 44, 773, 785
MalI GATC 3 cut(s) 58, 81, 348
MboI GATC 3 cut(s) 56, 79, 346
MboII GAAGA 1 cut(s) 440
MfeI CAATTG 2 cut(s) 246, 309
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 2 cut(s) 567, 796
MluCI AATT 7 cut(s) 40, 246, 285, 309, 510, 553, 734
MlyI GAGTC 2 cut(s) 157, 644
MseI TTAA 5 cut(s) 276, 509, 537, 725, 810
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 1 cut(s) 339
MunI CAATTG 2 cut(s) 246, 309
Mva1269I GAATGC 1 cut(s) 760
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 4 cut(s) 33, 473, 482, 620
NdeII GATC 3 cut(s) 56, 79, 346
NlaIII CATG 5 cut(s) 353, 365, 533, 644, 691
NlaIV GGNNCC 2 cut(s) 261, 795
NmuCI GTSAC 1 cut(s) 44
NruI TCGCGA 1 cut(s) 453
PceI AGGCCT 1 cut(s) 435
PctI GAATGC 1 cut(s) 760
PfeI GAWTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 1 cut(s) 714
PleI GAGTC 2 cut(s) 157, 643
PpsI GAGTC 2 cut(s) 157, 643
Ppu21I YACGTR 1 cut(s) 785
PpuMI RGGWCCY 1 cut(s) 575
PshBI ATTAAT 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 261, 795
PspPI GGNCC 2 cut(s) 475, 575
PspPPI RGGWCCY 1 cut(s) 575
PsuI RGATCY 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 36
RruI TCGCGA 1 cut(s) 453
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SacI GAGCTC 1 cut(s) 567
SalI GTCGAC 1 cut(s) 93
SaqAI TTAA 5 cut(s) 276, 509, 537, 725, 810
SatI GCNGC 1 cut(s) 713
Sau3AI GATC 3 cut(s) 56, 79, 346
Sau96I GGNCC 2 cut(s) 475, 575
SchI GAGTC 2 cut(s) 157, 644
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 2 cut(s) 567, 796
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 7 cut(s) 40, 246, 285, 309, 510, 553, 734
SseBI AGGCCT 1 cut(s) 435
SspI AATATT 1 cut(s) 7
SspMI CTAG 2 cut(s) 410, 611
SstI GAGCTC 1 cut(s) 567
StuI AGGCCT 1 cut(s) 435
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 615
TaiI ACGT 1 cut(s) 787
TaqI TCGA 1 cut(s) 94
TasI AATT 7 cut(s) 40, 246, 285, 309, 510, 553, 734
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 5 cut(s) 276, 509, 537, 725, 810
Tru9I TTAA 5 cut(s) 276, 509, 537, 725, 810
TscAI CASTG 1 cut(s) 261
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 1 cut(s) 712
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 4 cut(s) 17, 116, 555, 767
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 575
VspI ATTAAT 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmiI GTMKAC 1 cut(s) 94
XspI CTAG 2 cut(s) 410, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.