RLG00000023776

33 kDa ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013151
Physical Location & Seq
Forward (+)
28074508 .. 28076170
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023776

Sequence Viewer

Length: 1089 bp
ATGTCGACCTCTTCTCTATCAATGGCTTCCTCAAGTACTACTTGCTCTTCTTCACTTTGCAACAAAATCTCTAACTTCTCACTCACCCCAATCCCCACCCACTTACAACCTCAATTTAAACCCCCTAAACCCCTTAAACCCCTGAAGCTGAAAGCCCATTTCTCCTACTTCTCTCGTCTCTCTTCTCTCCACCATTTCCGCCGCCCCTTTTCGGCCTTAGACGGCCTCGAAGTCGAAGAAGGCAATGAAAGCTCGGAGGAAGAAAGAGAAGAACAAAAGGACCCACAAGCAGAATTGCTTGAAGAAGAAGAAGACAAAGTAGAAGAAGAAAAAGAAGAGCCAAAGGAAGCAGGGTCTGACAGAAGTGACGCTGGGAGGCTCTATGTGGGGAACTTGCCTTATTCTATGACTTCTACCCAGTTGTCTGAGATTTTCGGCGAGGCCGGTACCGTCACTTATTCAGAGCTCCACATTCGGTGTCCCTTTGAACTTCGAATGAGGGTTAATCCGAGATCCTATGCTAGAGGGCAAGTAGGCGGCCGAACTGTGAGGGTAAACTTCCCAGAAGTGCCGAGAGGGGGTGAAAGGGAAATTTTGGGACCCAAGATAAGGAATCCTGGCTACAAGGTCTACATAGATAGCCCTAATAAGATCTATGCAGGAAACCTTGGTTGGGGCCTGACTTCCCAGGGTCTGAAAGATGCCTTTGAAGGCCAGCCAGGGCTATTGGGTGCCAAGGTCATTTATGAGAGGGGTTCAGGAAGATCTCGAGGTTTTGGATTCGTCACTTTCGACACTGCTGCCGCTGCAGAAGCTGCTGTGACTGCCATGAACGGAGTGGAGGTTGATGGGCGGGCTTTGCGACTAAACATGGCTTCAGAAAGAGCTAGGACTGTAACTGTTACTCCACCTCCAGCATCAGAAACTAGTACAGAAAACACAGAGGCCAGTGAATTGGTTTCTCCCTCAGCATCTGAAACTACTGCAGAAGACGCTAAGGACAGTGGTGAAGTGGTTTCTTCCCCAGCATCAGAAACAACTGCAGAAGATGCAGACAGCAGTGAGTTGATTTCCAGCGTTAGCATCTGA

Protein Analysis

363

Amino Acids

39.24

Weight (kDa)

4.96

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 218 - 288 2.6e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013151)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52380
fragaria_vesca FvH4_6g20490 FvH4_6g20490
malus_domestica MD12G1042000.v1.1 MD14G1041200.v1.1
prunus_persica Prupe.7G093200_v2.0.a1
pyrus_communis pycom12g03670 pycom14g03500
rosa_chinensis RchiOBHm_Chr3g0476371
rosa_laevigata RLG00000023776
rosa_multiflora Rmu_sc0000114.1_g000008
rosa_roxburghii Rroxscaffold_6G00405360
rosa_rugosa Rorug03G0153800
rosa_samantha Rh3AG205000 Rh3BG236600 Rh3CG231100 Rh3DG230900
rosa_wichuraiana Rw3G018630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 446
AccB1I GGYRCC 2 cut(s) 446, 731
AccI GTMKAC 2 cut(s) 5, 630
AciI CCGC 5 cut(s) 199, 202, 537, 804, 853
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 2 cut(s) 164, 861
AfaI GTAC 3 cut(s) 37, 448, 931
AfiI CCNNNNNNNGG 4 cut(s) 211, 578, 609, 673
AgsI TTSAA 3 cut(s) 302, 488, 710
AhlI ACTAGT 1 cut(s) 926
AjnI CCWGG 3 cut(s) 616, 687, 718
AjuI GAANNNNNNNTTGG 2 cut(s) 655, 687
AluBI AGCT 5 cut(s) 148, 252, 466, 815, 887
AluI AGCT 5 cut(s) 148, 252, 466, 815, 887
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 507
AlwNI CAGNNNCTG 4 cut(s) 356, 694, 815, 974
Ama87I CYCGRG 1 cut(s) 768
AoxI GGCC 7 cut(s) 213, 223, 441, 538, 676, 712, 945
ApeKI GCWGC 3 cut(s) 800, 806, 815
ApoI RAATTY 1 cut(s) 591
Asp718I GGTACC 1 cut(s) 446
AspS9I GGNCC 3 cut(s) 280, 599, 676
AsuHPI GGTGA 3 cut(s) 76, 593, 1019
AsuII TTCGAA 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 768
AvaII GGWCC 2 cut(s) 280, 599
BanI GGYRCC 2 cut(s) 446, 731
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 2 cut(s) 318, 996
Bbv12I GWGCWC 1 cut(s) 468
BbvCI CCTCAGC 1 cut(s) 967
BbvI GCAGC 3 cut(s) 787, 793, 802
BccI CCATC 1 cut(s) 842
BceAI ACGGC 1 cut(s) 238
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BciT130I CCWGG 3 cut(s) 618, 689, 720
BcoDI GTCTC 1 cut(s) 182
BcuI ACTAGT 1 cut(s) 926
BfaI CTAG 3 cut(s) 522, 888, 927
BfmI CTRYAG 3 cut(s) 807, 984, 1041
BglII AGATCT 2 cut(s) 651, 764
BisI GCNGC 6 cut(s) 202, 538, 801, 804, 807, 816
BlsI GCNGC 6 cut(s) 203, 539, 802, 805, 808, 817
BmcAI AGTACT 1 cut(s) 37
Bme1390I CCNGG 3 cut(s) 618, 689, 720
Bme18I GGWCC 2 cut(s) 280, 599
BmeT110I CYCGRG 1 cut(s) 768
BmgT120I GGNCC 3 cut(s) 280, 599, 676
BmiI GGNNCC 6 cut(s) 282, 448, 600, 601, 677, 733
BmrFI CCNGG 3 cut(s) 618, 689, 720
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 5 cut(s) 691, 926, 980, 1037, 1039
BmuI ACTGGG 1 cut(s) 412
BpiI GAAGAC 2 cut(s) 318, 996
BpmI CTGGAG 1 cut(s) 897
Bpu10I CCTNAGC 2 cut(s) 967, 996
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 1 cut(s) 16
BsaJI CCNNGG 5 cut(s) 667, 687, 688, 719, 735
BsaXI ACNNNNNCTCC 6 cut(s) 828, 858, 889, 895, 919, 925
Bsc4I CCNNNNNNNGG 4 cut(s) 211, 578, 609, 673
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 2 cut(s) 418, 948
Bse3DI GCAATG 1 cut(s) 250
BseBI CCWGG 3 cut(s) 618, 689, 720
BseDI CCNNGG 5 cut(s) 667, 687, 688, 719, 735
BseLI CCNNNNNNNGG 4 cut(s) 211, 578, 609, 673
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 2 cut(s) 417, 981
BseNI ACTGG 2 cut(s) 418, 948
BseX3I CGGCCG 1 cut(s) 538
BseXI GCAGC 3 cut(s) 787, 793, 802
BseYI CCCAGC 2 cut(s) 371, 1024
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 7 cut(s) 215, 225, 443, 540, 678, 714, 947
BshNI GGYRCC 2 cut(s) 446, 731
BsiEI CGRYCG 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 768
BsiSI CCGG 1 cut(s) 444
BslFI GGGAC 2 cut(s) 465, 612
BslI CCNNNNNNNGG 4 cut(s) 211, 578, 609, 673
BsmAI GTCTC 1 cut(s) 182
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 465, 612
BsnI GGCC 7 cut(s) 215, 225, 443, 540, 678, 714, 947
BsoBI CYCGRG 1 cut(s) 768
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 3 cut(s) 512, 651, 764
BspACI CCGC 5 cut(s) 199, 202, 537, 804, 853
BspANI GGCC 7 cut(s) 215, 225, 443, 540, 678, 714, 947
BspCNI CTCAG 2 cut(s) 418, 980
BspLI GGNNCC 6 cut(s) 282, 448, 600, 601, 677, 733
BspMAI CTGCAG 3 cut(s) 811, 988, 1045
BspPI GGATC 1 cut(s) 507
BspQI GCTCTTC 2 cut(s) 52, 330
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 2 cut(s) 446, 731
BsrDI GCAATG 1 cut(s) 250
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 2 cut(s) 418, 948
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 5 cut(s) 667, 687, 688, 719, 735
BssMI GATC 3 cut(s) 512, 651, 764
BssT1I CCWWGG 2 cut(s) 667, 735
Bst2UI CCWGG 3 cut(s) 618, 689, 720
Bst4CI ACNGT 5 cut(s) 451, 547, 895, 901, 1004
Bst6I CTCTTC 4 cut(s) 16, 52, 187, 330
BstAPI GCANNNNNTGC 2 cut(s) 815, 1049
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 2 cut(s) 716, 855
BstDEI CTNAG 4 cut(s) 217, 426, 967, 996
BstKTI GATC 3 cut(s) 515, 654, 767
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 3 cut(s) 512, 651, 764
BstMCI CGRYCG 1 cut(s) 541
BstMWI GCNNNNNNNGC 8 cut(s) 249, 806, 812, 815, 824, 859, 992, 1049
BstNI CCWGG 3 cut(s) 618, 689, 720
BstSCI CCNGG 3 cut(s) 616, 687, 718
BstSFI CTRYAG 3 cut(s) 807, 984, 1041
BstV1I GCAGC 3 cut(s) 787, 793, 802
BstV2I GAAGAC 2 cut(s) 318, 996
BstX2I RGATCY 3 cut(s) 512, 651, 764
BstXI CCANNNNNNTGG 1 cut(s) 955
BstYI RGATCY 3 cut(s) 512, 651, 764
BstZI CGGCCG 1 cut(s) 538
BsuRI GGCC 7 cut(s) 215, 225, 443, 540, 678, 714, 947
BtsI GCAGTG 2 cut(s) 795, 1066
BtsIMutI CAGTG 4 cut(s) 795, 955, 1009, 1066
Cac8I GCNNGC 2 cut(s) 716, 855
CaiI CAGNNNCTG 4 cut(s) 356, 694, 815, 974
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 3 cut(s) 280, 599, 676
CseI GACGC 2 cut(s) 377, 1001
Csp6I GTAC 3 cut(s) 36, 447, 930
CviAII CATG 2 cut(s) 829, 871
CviQI GTAC 3 cut(s) 36, 447, 930
DdeI CTNAG 4 cut(s) 217, 426, 967, 996
DpnI GATC 3 cut(s) 514, 653, 766
DpnII GATC 3 cut(s) 512, 651, 764
DraI TTTAAA 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 538
EagI CGGCCG 1 cut(s) 538
Eam1104I CTCTTC 4 cut(s) 16, 52, 187, 330
EarI CTCTTC 4 cut(s) 16, 52, 187, 330
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 466
EclXI CGGCCG 1 cut(s) 538
Eco130I CCWWGG 2 cut(s) 667, 735
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 280, 599
Eco52I CGGCCG 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 2 cut(s) 164, 861
Eco88I CYCGRG 1 cut(s) 768
EcoICRI GAGCTC 1 cut(s) 466
EcoO109I RGGNCCY 3 cut(s) 280, 599, 676
EcoRII CCWGG 3 cut(s) 616, 687, 718
EcoT14I CCWWGG 2 cut(s) 667, 735
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 2 cut(s) 667, 735
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 2 cut(s) 832, 874
FaiI YATR 8 cut(s) 384, 407, 519, 635, 657, 747, 830, 872
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FaqI GGGAC 2 cut(s) 465, 612
FatI CATG 2 cut(s) 828, 870
FauI CCCGC 1 cut(s) 846
FblI GTMKAC 2 cut(s) 5, 630
Fnu4HI GCNGC 6 cut(s) 202, 538, 801, 804, 807, 816
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 6 cut(s) 202, 538, 801, 804, 807, 816
FspBI CTAG 3 cut(s) 522, 888, 927
GluI GCNGC 6 cut(s) 202, 538, 801, 804, 807, 816
GsaI CCCAGC 2 cut(s) 375, 1028
GsuI CTGGAG 1 cut(s) 897
HaeIII GGCC 7 cut(s) 215, 225, 443, 540, 678, 714, 947
HapII CCGG 1 cut(s) 444
HgaI GACGC 2 cut(s) 377, 1001
Hin1II CATG 2 cut(s) 832, 874
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 613, 780
HpaII CCGG 1 cut(s) 444
HphI GGTGA 3 cut(s) 76, 593, 1019
Hpy166II GTNNAC 3 cut(s) 6, 556, 631
Hpy188III TCNNGA 2 cut(s) 759, 768
Hpy8I GTNNAC 3 cut(s) 6, 556, 631
HpyAV CCTTC 2 cut(s) 233, 704
HpyCH4III ACNGT 5 cut(s) 451, 547, 895, 901, 1004
HpyCH4V TGCA 6 cut(s) 60, 659, 809, 986, 1043, 1052
HpyF10VI GCNNNNNNNGC 8 cut(s) 249, 806, 812, 815, 824, 859, 992, 1049
HpyF3I CTNAG 4 cut(s) 217, 426, 967, 996
Hsp92II CATG 2 cut(s) 832, 874
KflI GGGWCCC 1 cut(s) 599
KpnI GGTACC 1 cut(s) 450
Kzo9I GATC 3 cut(s) 512, 651, 764
LguI GCTCTTC 2 cut(s) 52, 330
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 3 cut(s) 787, 793, 802
LweI GCATC 5 cut(s) 691, 926, 980, 1037, 1039
MaeI CTAG 3 cut(s) 522, 888, 927
MaeIII GTNAC 6 cut(s) 365, 451, 784, 820, 895, 901
MalI GATC 3 cut(s) 514, 653, 766
MboI GATC 3 cut(s) 512, 651, 764
MflI RGATCY 3 cut(s) 512, 651, 764
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 4 cut(s) 113, 293, 591, 953
MseI TTAA 3 cut(s) 117, 135, 504
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 1 cut(s) 444
MspR9I CCNGG 3 cut(s) 618, 689, 720
MvaI CCWGG 3 cut(s) 618, 689, 720
MwoI GCNNNNNNNGC 8 cut(s) 249, 806, 812, 815, 824, 859, 992, 1049
NdeII GATC 3 cut(s) 512, 651, 764
NlaIII CATG 2 cut(s) 832, 874
NlaIV GGNNCC 6 cut(s) 282, 448, 600, 601, 677, 733
NmeAIII GCCGAG 1 cut(s) 597
NmuCI GTSAC 4 cut(s) 365, 451, 784, 820
NspV TTCGAA 1 cut(s) 493
PaeR7I CTCGAG 1 cut(s) 768
PasI CCCWGGG 1 cut(s) 688
PciSI GCTCTTC 2 cut(s) 52, 330
PcsI WCGNNNNNNNCGW 1 cut(s) 789
PfeI GAWTC 2 cut(s) 613, 780
PkrI GCNGC 6 cut(s) 203, 539, 802, 805, 808, 817
PpuMI RGGWCCY 2 cut(s) 280, 599
Psp124BI GAGCTC 1 cut(s) 468
Psp5II RGGWCCY 2 cut(s) 280, 599
Psp6I CCWGG 3 cut(s) 616, 687, 718
PspFI CCCAGC 2 cut(s) 371, 1024
PspGI CCWGG 3 cut(s) 616, 687, 718
PspN4I GGNNCC 6 cut(s) 282, 448, 600, 601, 677, 733
PspPI GGNCC 3 cut(s) 280, 599, 676
PspPPI RGGWCCY 2 cut(s) 280, 599
PstI CTGCAG 3 cut(s) 811, 988, 1045
PstNI CAGNNNCTG 4 cut(s) 356, 694, 815, 974
PsuI RGATCY 3 cut(s) 512, 651, 764
RsaI GTAC 3 cut(s) 37, 448, 931
RsaNI GTAC 3 cut(s) 36, 447, 930
SacI GAGCTC 1 cut(s) 468
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 2 cut(s) 52, 330
SaqAI TTAA 3 cut(s) 117, 135, 504
SatI GCNGC 6 cut(s) 202, 538, 801, 804, 807, 816
Sau3AI GATC 3 cut(s) 512, 651, 764
Sau96I GGNCC 3 cut(s) 280, 599, 676
ScaI AGTACT 1 cut(s) 37
ScrFI CCNGG 3 cut(s) 618, 689, 720
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 5 cut(s) 691, 926, 980, 1037, 1039
SfcI CTRYAG 3 cut(s) 807, 984, 1041
Sfr274I CTCGAG 1 cut(s) 768
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 2 cut(s) 280, 599
SlaI CTCGAG 1 cut(s) 768
SmlI CTYRAG 2 cut(s) 31, 768
SmoI CTYRAG 2 cut(s) 31, 768
SpeI ACTAGT 1 cut(s) 926
Sse9I AATT 4 cut(s) 113, 293, 591, 953
SsiI CCGC 5 cut(s) 199, 202, 537, 804, 853
SspMI CTAG 3 cut(s) 522, 888, 927
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 3 cut(s) 616, 687, 718
StyI CCWWGG 2 cut(s) 667, 735
TaaI ACNGT 5 cut(s) 451, 547, 895, 901, 1004
TaqI TCGA 6 cut(s) 5, 228, 234, 493, 769, 792
TasI AATT 4 cut(s) 113, 293, 591, 953
TatI WGTACW 2 cut(s) 35, 929
TauI GCSGC 3 cut(s) 204, 540, 806
TfiI GAWTC 2 cut(s) 613, 780
Tru1I TTAA 3 cut(s) 117, 135, 504
Tru9I TTAA 3 cut(s) 117, 135, 504
TscAI CASTG 4 cut(s) 802, 955, 1009, 1066
TseFI GTSAC 4 cut(s) 365, 451, 784, 820
TseI GCWGC 3 cut(s) 800, 806, 815
Tsp45I GTSAC 4 cut(s) 365, 451, 784, 820
TspDTI ATGAA 2 cut(s) 261, 845
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 4 cut(s) 802, 955, 1009, 1066
VpaK11BI GGWCC 2 cut(s) 280, 599
XapI RAATTY 1 cut(s) 591
XcmI CCANNNNNNNNNTGG 1 cut(s) 835
XhoI CTCGAG 1 cut(s) 768
XmiI GTMKAC 2 cut(s) 5, 630
XspI CTAG 3 cut(s) 522, 888, 927
ZrmI AGTACT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.