Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0017189

Physical Location & Seq

Genomic Coordinates Reverse (-)

35603405 .. 35605645

Gene Structure 2241 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024415

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2241 bp

ATGAACCTCAAACCACCTCCACCATCTTCACCATTACCCACCAATTCAAACCCACTCCCTCTAACCCACACCCTCACTCTCTACTGGGTCTCTCCCCCGCCTTTTCCCTCTGCCATTTCCAAACCCACGCGCCCTTTTCGCCTCCACGCCACCGCCGCCAACCCCGCCAACCCAGCCGCAGAGCTAACTCTCACAACCTCCGCCGCCGCCGACGACGACGAGCCACCGATATCTAATGAAGACCAGAAGCTTCTAATCCTCCTCCGCCAGAGAAAAACAGAAGAGGCTTGGATTCTCTACACCCAATCCTCCCGCCTCCCCAATCCCACTTGCCTCAGCCGCTTGCTCTCCCAGCTGTCCTACCAGAACACTCGCACCAGCCTCACGCGCGCCCAGTCCATCATCACGCGCCTTCGCAATGAGCGCCAGCTCCGCCACCTCGACGCCAACTCCCTCGGCCTCCTCGCCGTCGCCGCCTCCAAGGCCGGCCAGACCCGCTACGCCACCTCCATCGTCAAGTCCATGCTCCGCTCCGGCTTCCTCCCCCACGTCAAGGCCTGGAGCGCCGTCGTCAGCCGCCTCGCTGCCTCCGGCGACGACGGCCCTTCCGAAGCGCTCAAGCTCTTCTATTCCGTCACGCGCCGCGTCCGGAGGTTCGCCGAGCCGGAACTGGTGGCGGACTCGCGGCCGGACACGGCGGCGTTCAATGCTGCGCTGAATGCATGTGCTAATTTGGGTGATACAGATAGGTTCTTGAAGCTGTTCGACGAAATGCCTGAGTCAGGTGCCGAGCCGGATGTGCTTACCTATAATGTGATGATCAAGCTTTGCGCTAGAGTTGGAAGGATGGACTTGCTTGTTTTTGTGTTGGAGAGGATTTTGGAGCAGGGGATCACAGTGTGTATGACTACATTGAATTCCCTGGTTGCGGCTTATGTTGGTTTCGGAGACTTGGAAACTGCAGAGAGGATGGTTCAGGCAATGAGGGAAGGGAGGAGAGACCTCTGCAAGATTCTGAGGGAGGCTAATTTGAGGAATTCGAATTCTAGTGGAAGAGATGGGGATGTGTTTGAGAGGTTGCTTCCGAATTCAGTTACAGCTAGTGAATGTGAGCCGCCATTGTTGCCAAAAGCGTATACTTCTAACTCGAGGATTTACACTACACTGATGAAGGGTTATATGAATGCTGGGCGTGTTACTGACACGGTTCGGATGCTTGAGGCACTGAGGCACCAGGATGATAGCTCGAGTCAGCCTGACCATGTGACATATACTACTGTTGTTTCTGCATTTGTGAAGGCGGGTTCCATGGACCGTGCTCGCAGGGTTCTAGCTGAGATGACGAGAGTTGGCGTGCCTGCAAATCGGATAACGTATAACATTCTTCTCAAGGGTTATTGTCAGCAGCTGCAGATAGACCAGGCAAAGGAGCTGGTTAGAGAGATGACTGAGGATGCGGGGATTGAGCCTGATGTGGTTTCGTATAACATCTTGATCGATGGGTGCATACAGGTTGATGACAGTGCTGGTGCTCTTGCTTTCTTCAATGAAATGAGAGCAAAAGGAATTGCTCCCACCAAGATCAGCTACACCACTTTGATGAAAGCTTTTGCCTTGTCTGGACAGCCAAAGCTGGCTAACAAGGTATTCGAAGAAATGCTTAACGATCCTCGGGTGAAGGCTGATTTGGTAGCTTGGAACATGTTGGTCGAAGGGTATTGCAGACTGGGGTTGGTTGAAGAATCCAAGAAAATCATTCAGAGAATGAAAGAGCATGGGTTTCACCCTGATGTGGCTACTTATGGTAGCCTAGCTAATGGGATAGCATTGGCCAGAAAACCGGGAGAGGCACTTTTGCTTTGGAATGAAGTGAAGGACAGATGTACAGCGAAAAAGGAAGGTGAAAAATCTGATGATTCAGATCCTCCAACATTGAAACCTGATGAAGGGCTATTGGATACTTTGGCTGATATTTGTGTTAGGGCTGCTTTCTTCAAGAAGGCTCTGGAAATTGTCGCTTGTATGGAAGAGAATGGGATTCCTCCAAACAAAACCAAGTTCACTAAAATCTATGTGGAGATGCATTCGAGGATGTTTACTAGTAAGCACGCCTCACAAGCTAGGCAGGACAGGAGGAGTGAAAGGAAGAGAGCAGCTGAGGCTTTCAAGTTTTGGTTGGGTTTACCCAATTCTTATTATGGGAGTGAATGGAGGTTAGGATCTTTGGATGGAGATAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

747

Amino Acids

82.85

Weight (kDa)

8.63

Isoelectric Point (pI)

41.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_At1g68980	PF25245	81 - 196	1.2e-06	At1g68980, TPR repeats
PPR_3	PF13812	229 - 277	1.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	230 - 279	4.9e-12	PPR repeat family
TPR_24	PF23276	396 - 517	3.7e-09	Fungal tetratrico peptide repeats
PPR_long	PF17177	403 - 504	2.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	418 - 448	1.2e-06	PPR repeat
PPR_3	PF13812	418 - 466	1.7e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	419 - 468	9.1e-14	PPR repeat family
PPR_1	PF12854	451 - 483	4.6e-08	PPR repeat
PPR_2	PF13041	455 - 503	2.9e-14	PPR repeat family
PPR_3	PF13812	478 - 537	5.1e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	490 - 538	4.4e-14	PPR repeat family
PPR_2	PF13041	562 - 608	2.1e-10	PPR repeat family
PPR	PF01535	564 - 594	4.5e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017189)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09650
fragaria_vesca	FvH4_6g15290
prunus_persica	Prupe.6G241600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0468701
rosa_laevigata	RLG00000024415
rosa_multiflora	Rmu_sc0011538.1_g000002 Rmu_sc0028512.1_g000001 Rmu_sc0033138.1_g000003
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145300 Rh3BG168000 Rh3DG166600
rosa_wichuraiana	Rw3G013690

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	785, 1230
AccBSI	CCGCTC	1 cut(s)	531
AccI	GTMKAC	1 cut(s)	1136
AccII	CGCG	7 cut(s)	130, 388, 390, 409, 640, 645, 685
AccIII	TCCGGA	1 cut(s)	648
AclWI	GGATC	4 cut(s)	899, 1661, 1916, 2227
AcoI	YGGCCR	3 cut(s)	487, 686, 1830
AcsI	RAATTY	4 cut(s)	916, 1036, 1042, 1087
AcyI	GRCGYC	1 cut(s)	444
AdeI	CACNNNGTG	1 cut(s)	900
AfaI	GTAC	1 cut(s)	1885
AfeI	AGCGCT	1 cut(s)	615
AfiI	CCNNNNNNNGG	6 cut(s)	553, 782, 928, 1426, 1792, 1946
AflIII	ACRYGT	1 cut(s)	1701
AgsI	TTSAA	9 cut(s)	48, 706, 757, 916, 1546, 1739, 1936, 1996, 2167
AhlI	ACTAGT	1 cut(s)	2099
AjiI	CACGTC	1 cut(s)	550
AjnI	CCWGG	4 cut(s)	557, 921, 1233, 1419
AjuI	GAANNNNNNNTTGG	2 cut(s)	1670, 1702
Alw21I	GWGCWC	2 cut(s)	1321, 1534
Alw26I	GTCTC	3 cut(s)	94, 942, 993
AlwI	GGATC	4 cut(s)	899, 1661, 1916, 2227
AlwNI	CAGNNNCTG	1 cut(s)	1408
Ama87I	CYCGRG	3 cut(s)	1147, 1246, 1671
Aor13HI	TCCGGA	1 cut(s)	648
Aor51HI	AGCGCT	1 cut(s)	615
AoxI	GGCC	7 cut(s)	457, 483, 487, 555, 601, 686, 1830
ApeKI	GCWGC	6 cut(s)	584, 710, 1405, 1408, 1985, 2153
ApoI	RAATTY	4 cut(s)	916, 1036, 1042, 1087
Asp700I	GAANNNNTTC	1 cut(s)	761
AspS9I	GGNCC	2 cut(s)	602, 1312
AsuC2I	CCSGG	1 cut(s)	1842
AsuHPI	GGTGA	5 cut(s)	21, 749, 1687, 1775, 1913
AsuII	TTCGAA	2 cut(s)	1040, 1650
AvaI	CYCGRG	3 cut(s)	1147, 1246, 1671
AvaII	GGWCC	1 cut(s)	1312
BalI	TGGCCA	1 cut(s)	1832
BanI	GGYRCC	2 cut(s)	785, 1230
BbsI	GAAGAC	1 cut(s)	246
Bbv12I	GWGCWC	2 cut(s)	1321, 1534
BbvCI	CCTCAGC	2 cut(s)	335, 2157
BbvI	GCAGC	6 cut(s)	571, 697, 1395, 1417, 1972, 2165
BccI	CCATC	8 cut(s)	31, 407, 518, 841, 964, 1052, 1493, 2222
BceAI	ACGGC	4 cut(s)	452, 551, 616, 711
BciT130I	CCWGG	4 cut(s)	559, 923, 1235, 1421
BciVI	GTATCC	1 cut(s)	1951
BclI	TGATCA	1 cut(s)	819
BcnI	CCSGG	1 cut(s)	1842
BcoDI	GTCTC	3 cut(s)	94, 942, 993
BcuI	ACTAGT	1 cut(s)	2099
BfaI	CTAG	7 cut(s)	834, 1047, 1101, 1331, 1811, 2100, 2121
BfmI	CTRYAG	2 cut(s)	960, 1409
BfoI	RGCGCY	3 cut(s)	427, 567, 617
BfuI	GTATCC	1 cut(s)	1951
BglI	GCCNNNNNGGC	1 cut(s)	482
Bme1390I	CCNGG	5 cut(s)	559, 923, 1235, 1421, 1842
Bme18I	GGWCC	1 cut(s)	1312
BmeT110I	CYCGRG	3 cut(s)	1147, 1246, 1671
BmgBI	CACGTC	1 cut(s)	550
BmgT120I	GGNCC	2 cut(s)	602, 1312
BmiI	GGNNCC	3 cut(s)	787, 1232, 1306
BmrFI	CCNGG	5 cut(s)	559, 923, 1235, 1421, 1842
BmrI	ACTGGG	3 cut(s)	94, 388, 1736
BmsI	GCATC	3 cut(s)	1203, 1444, 2070
BmuI	ACTGGG	3 cut(s)	94, 388, 1736
BpiI	GAAGAC	1 cut(s)	246
BpmI	CTGGAG	1 cut(s)	580
Bpu10I	CCTNAGC	2 cut(s)	335, 2157
Bpu14I	TTCGAA	2 cut(s)	1040, 1650
BpuEI	CTTGAG	3 cut(s)	602, 1238, 1373
BpuMI	CCSGG	1 cut(s)	1842
Bsa29I	ATCGAT	1 cut(s)	1497
BsaBI	GATNNNNATC	1 cut(s)	1920
BsaHI	GRCGYC	1 cut(s)	444
BsaI	GGTCTC	2 cut(s)	94, 993
BsaJI	CCNNGG	5 cut(s)	454, 480, 921, 1308, 1670
BsaWI	WCCGGW	1 cut(s)	648
BsaXI	ACNNNNNCTCC	4 cut(s)	434, 464, 491, 521
Bsc4I	CCNNNNNNNGG	6 cut(s)	553, 782, 928, 1426, 1792, 1946
Bse118I	RCCGGY	1 cut(s)	485
Bse1I	ACTGG	4 cut(s)	89, 394, 675, 1731
Bse3DI	GCAATG	2 cut(s)	424, 987
Bse8I	GATNNNNATC	1 cut(s)	1920
BseAI	TCCGGA	1 cut(s)	648
BseBI	CCWGG	4 cut(s)	559, 923, 1235, 1421
BseCI	ATCGAT	1 cut(s)	1497
BseDI	CCNNGG	5 cut(s)	454, 480, 921, 1308, 1670
BseGI	GGATG	9 cut(s)	802, 852, 975, 1069, 1218, 1243, 1459, 2097, 2233
BseJI	GATNNNNATC	1 cut(s)	1920
BseLI	CCNNNNNNNGG	6 cut(s)	553, 782, 928, 1426, 1792, 1946
BseMI	GCAATG	2 cut(s)	424, 987
BseMII	CTCAG	7 cut(s)	349, 768, 1007, 1217, 1326, 1440, 2148
BseNI	ACTGG	4 cut(s)	89, 394, 675, 1731
BsePI	GCGCGC	1 cut(s)	388
BseRI	GAGGAG	4 cut(s)	251, 452, 1009, 2149
BseX3I	CGGCCG	1 cut(s)	686
BseXI	GCAGC	6 cut(s)	571, 697, 1395, 1417, 1972, 2165
BseYI	CCCAGC	3 cut(s)	172, 351, 1187
Bsh1236I	CGCG	7 cut(s)	130, 388, 390, 409, 640, 645, 685
Bsh1285I	CGRYCG	1 cut(s)	689
BshFI	GGCC	7 cut(s)	459, 485, 489, 557, 603, 688, 1832
BshNI	GGYRCC	2 cut(s)	785, 1230
BshVI	ATCGAT	1 cut(s)	1497
BsiEI	CGRYCG	1 cut(s)	689
BsiHKAI	GWGCWC	2 cut(s)	1321, 1534
BsiHKCI	CYCGRG	3 cut(s)	1147, 1246, 1671
BsiSI	CCGG	8 cut(s)	486, 534, 591, 649, 665, 689, 794, 1841
BslI	CCNNNNNNNGG	6 cut(s)	553, 782, 928, 1426, 1792, 1946
BsmAI	GTCTC	3 cut(s)	94, 942, 993
BsmI	GAATGC	3 cut(s)	724, 1189, 2083
BsnI	GGCC	7 cut(s)	459, 485, 489, 557, 603, 688, 1832
Bso31I	GGTCTC	2 cut(s)	94, 993
BsoBI	CYCGRG	3 cut(s)	1147, 1246, 1671
Bsp119I	TTCGAA	2 cut(s)	1040, 1650
Bsp1286I	GDGCHC	2 cut(s)	1321, 1534
Bsp13I	TCCGGA	1 cut(s)	648
Bsp1407I	TGTACA	1 cut(s)	1883
Bsp143I	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
Bsp19I	CCATGG	1 cut(s)	1308
BspANI	GGCC	7 cut(s)	459, 485, 489, 557, 603, 688, 1832
BspCNI	CTCAG	7 cut(s)	348, 769, 1008, 1218, 1327, 1441, 2149
BspDI	ATCGAT	1 cut(s)	1497
BspEI	TCCGGA	1 cut(s)	648
BspFNI	CGCG	7 cut(s)	130, 388, 390, 409, 640, 645, 685
BspLI	GGNNCC	3 cut(s)	787, 1232, 1306
BspMAI	CTGCAG	2 cut(s)	964, 1413
BspPI	GGATC	4 cut(s)	899, 1661, 1916, 2227
BspQI	GCTCTTC	1 cut(s)	629
BspT104I	TTCGAA	2 cut(s)	1040, 1650
BspT107I	GGYRCC	2 cut(s)	785, 1230
BspTNI	GGTCTC	2 cut(s)	94, 993
BsrBI	CCGCTC	1 cut(s)	531
BsrDI	GCAATG	2 cut(s)	424, 987
BsrFI	RCCGGY	1 cut(s)	485
BsrGI	TGTACA	1 cut(s)	1883
BsrI	ACTGG	4 cut(s)	89, 394, 675, 1731
BssAI	RCCGGY	1 cut(s)	485
BssECI	CCNNGG	5 cut(s)	454, 480, 921, 1308, 1670
BssHII	GCGCGC	1 cut(s)	388
BssMI	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
BssNAI	GTATAC	1 cut(s)	1137
BssNI	GRCGYC	1 cut(s)	444
BssT1I	CCWWGG	2 cut(s)	480, 1308
Bst1107I	GTATAC	1 cut(s)	1137
Bst2UI	CCWGG	4 cut(s)	559, 923, 1235, 1421
Bst4CI	ACNGT	5 cut(s)	898, 1207, 1279, 1316, 1523
Bst6I	CTCTTC	5 cut(s)	276, 629, 1048, 2022, 2141
BstACI	GRCGYC	1 cut(s)	444
BstAUI	TGTACA	1 cut(s)	1883
BstBI	TTCGAA	2 cut(s)	1040, 1650
BstC8I	GCNNGC	9 cut(s)	344, 390, 428, 487, 1321, 1355, 1359, 1635, 2109
BstDEI	CTNAG	7 cut(s)	335, 777, 1016, 1226, 1335, 1449, 2157
BstDSI	CCRYGG	1 cut(s)	1308
BstENI	CCTNNNNNAGG	2 cut(s)	780, 1944
BstF5I	GGATG	9 cut(s)	802, 852, 975, 1069, 1218, 1243, 1459, 2097, 2233
BstFNI	CGCG	7 cut(s)	130, 388, 390, 409, 640, 645, 685
BstH2I	RGCGCY	3 cut(s)	427, 567, 617
BstKTI	GATC	7 cut(s)	822, 894, 1497, 1584, 1669, 1924, 2222
BstMAI	GTCTC	3 cut(s)	94, 942, 993
BstMBI	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
BstMCI	CGRYCG	1 cut(s)	689
BstNI	CCWGG	4 cut(s)	559, 923, 1235, 1421
BstNSI	RCATGY	2 cut(s)	726, 1705
BstSCI	CCNGG	5 cut(s)	557, 921, 1233, 1419, 1840
BstSFI	CTRYAG	2 cut(s)	960, 1409
BstUI	CGCG	7 cut(s)	130, 388, 390, 409, 640, 645, 685
BstV1I	GCAGC	6 cut(s)	571, 697, 1395, 1417, 1972, 2165
BstV2I	GAAGAC	1 cut(s)	246
BstX2I	RGATCY	2 cut(s)	1921, 2219
BstYI	RGATCY	2 cut(s)	1921, 2219
BstZ17I	GTATAC	1 cut(s)	1137
BstZI	CGGCCG	1 cut(s)	686
Bsu15I	ATCGAT	1 cut(s)	1497
BsuI	GTATCC	1 cut(s)	1951
BsuRI	GGCC	7 cut(s)	459, 485, 489, 557, 603, 688, 1832
BsuTUI	ATCGAT	1 cut(s)	1497
BtgI	CCRYGG	1 cut(s)	1308
BtrI	CACGTC	1 cut(s)	550
BtsCI	GGATG	9 cut(s)	802, 852, 975, 1069, 1218, 1243, 1459, 2097, 2233
BtsIMutI	CAGTG	4 cut(s)	903, 1163, 1223, 1528
Cac8I	GCNNGC	9 cut(s)	344, 390, 428, 487, 1321, 1355, 1359, 1635, 2109
CaiI	CAGNNNCTG	1 cut(s)	1408
Cfr10I	RCCGGY	1 cut(s)	485
Cfr13I	GGNCC	2 cut(s)	602, 1312
ClaI	ATCGAT	1 cut(s)	1497
CseI	GACGC	2 cut(s)	452, 634
Csp6I	GTAC	1 cut(s)	1884
CviAII	CATG	6 cut(s)	523, 723, 1262, 1309, 1702, 1775
CviQI	GTAC	1 cut(s)	1884
DdeI	CTNAG	7 cut(s)	335, 777, 1016, 1226, 1335, 1449, 2157
DpnI	GATC	7 cut(s)	821, 893, 1496, 1583, 1668, 1923, 2221
DpnII	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
DraIII	CACNNNGTG	1 cut(s)	900
EaeI	YGGCCR	3 cut(s)	487, 686, 1830
EagI	CGGCCG	1 cut(s)	686
Eam1104I	CTCTTC	5 cut(s)	276, 629, 1048, 2022, 2141
EarI	CTCTTC	5 cut(s)	276, 629, 1048, 2022, 2141
EciI	GGCGGA	4 cut(s)	190, 254, 422, 692
EclXI	CGGCCG	1 cut(s)	686
Eco130I	CCWWGG	2 cut(s)	480, 1308
Eco147I	AGGCCT	1 cut(s)	557
Eco31I	GGTCTC	2 cut(s)	94, 993
Eco32I	GATATC	1 cut(s)	231
Eco47I	GGWCC	1 cut(s)	1312
Eco47III	AGCGCT	1 cut(s)	615
Eco52I	CGGCCG	1 cut(s)	686
Eco88I	CYCGRG	3 cut(s)	1147, 1246, 1671
EcoNI	CCTNNNNNAGG	2 cut(s)	780, 1944
EcoRI	GAATTC	4 cut(s)	916, 1036, 1042, 1087
EcoRII	CCWGG	4 cut(s)	557, 921, 1233, 1419
EcoRV	GATATC	1 cut(s)	231
EcoT14I	CCWWGG	2 cut(s)	480, 1308
EcoT22I	ATGCAT	2 cut(s)	724, 2085
ErhI	CCWWGG	2 cut(s)	480, 1308
FaeI	CATG	6 cut(s)	526, 726, 1265, 1312, 1705, 1778
FalI	AAGNNNNNCTT	2 cut(s)	1644, 1676
FatI	CATG	6 cut(s)	522, 722, 1261, 1308, 1701, 1774
FauI	CCCGC	6 cut(s)	105, 172, 320, 503, 1294, 1450
FbaI	TGATCA	1 cut(s)	819
FblI	GTMKAC	1 cut(s)	1136
FokI	GGATG	8 cut(s)	809, 859, 982, 1076, 1225, 1250, 1466, 2104
FseI	GGCCGGCC	1 cut(s)	489
FspBI	CTAG	7 cut(s)	834, 1047, 1101, 1331, 1811, 2100, 2121
GsaI	CCCAGC	3 cut(s)	176, 355, 1191
GsuI	CTGGAG	1 cut(s)	580
HaeII	RGCGCY	3 cut(s)	427, 567, 617
HaeIII	GGCC	7 cut(s)	459, 485, 489, 557, 603, 688, 1832
HapII	CCGG	8 cut(s)	486, 534, 591, 649, 665, 689, 794, 1841
HgaI	GACGC	2 cut(s)	452, 634
Hin1I	GRCGYC	1 cut(s)	444
Hin1II	CATG	6 cut(s)	526, 726, 1265, 1312, 1705, 1778
HindIII	AAGCTT	3 cut(s)	248, 824, 1605
HinfI	GANTC	8 cut(s)	292, 680, 779, 1012, 1249, 1742, 1916, 2038
HpaII	CCGG	8 cut(s)	486, 534, 591, 649, 665, 689, 794, 1841
HphI	GGTGA	5 cut(s)	21, 749, 1687, 1775, 1913
Hpy166II	GTNNAC	4 cut(s)	1137, 2061, 2097, 2183
Hpy188I	TCNGA	9 cut(s)	610, 947, 1017, 1086, 1212, 1368, 1761, 1912, 1921
Hpy188III	TCNNGA	6 cut(s)	649, 754, 1492, 1620, 1996, 2006
Hpy8I	GTNNAC	4 cut(s)	1137, 2061, 2097, 2183
Hpy99I	CGWCG	9 cut(s)	215, 218, 221, 446, 473, 572, 599, 602, 770
HpyCH4III	ACNGT	5 cut(s)	898, 1207, 1279, 1316, 1523
HpyCH4IV	ACGT	2 cut(s)	549, 1373
HpyCH4V	TGCA	9 cut(s)	722, 962, 1008, 1289, 1361, 1411, 1506, 1722, 2083
HpyF3I	CTNAG	7 cut(s)	335, 777, 1016, 1226, 1335, 1449, 2157
HpySE526I	ACGT	2 cut(s)	549, 1373
Hsp92I	GRCGYC	1 cut(s)	444
Hsp92II	CATG	6 cut(s)	526, 726, 1265, 1312, 1705, 1778
Kpn2I	TCCGGA	1 cut(s)	648
KroI	GCCGGC	1 cut(s)	485
KroNI	GCCGGC	1 cut(s)	487
Ksp22I	TGATCA	1 cut(s)	819
Kzo9I	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
LguI	GCTCTTC	1 cut(s)	629
LmnI	GCTCC	7 cut(s)	435, 531, 536, 561, 883, 1429, 1576
Lsp1109I	GCAGC	6 cut(s)	571, 697, 1395, 1417, 1972, 2165
LweI	GCATC	3 cut(s)	1203, 1444, 2070
MaeI	CTAG	7 cut(s)	834, 1047, 1101, 1331, 1811, 2100, 2121
MaeII	ACGT	2 cut(s)	549, 1373
MaeIII	GTNAC	4 cut(s)	634, 1093, 1195, 1264
MalI	GATC	7 cut(s)	821, 893, 1496, 1583, 1668, 1923, 2221
MbiI	CCGCTC	1 cut(s)	531
MboI	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
MflI	RGATCY	2 cut(s)	1921, 2219
MhlI	GDGCHC	2 cut(s)	1321, 1534
MlsI	TGGCCA	1 cut(s)	1832
MluNI	TGGCCA	1 cut(s)	1832
MlyI	GAGTC	3 cut(s)	674, 788, 1258
MmeI	TCCRAC	3 cut(s)	820, 849, 1952
Mox20I	TGGCCA	1 cut(s)	1832
Mph1103I	ATGCAT	2 cut(s)	724, 2085
MroI	TCCGGA	1 cut(s)	648
MroNI	GCCGGC	1 cut(s)	485
MroXI	GAANNNNTTC	1 cut(s)	761
MscI	TGGCCA	1 cut(s)	1832
MseI	TTAA	1 cut(s)	1662
MslI	CAYNNNNRTG	3 cut(s)	1236, 1598, 1788
Msp20I	TGGCCA	1 cut(s)	1832
MspA1I	CMGCKG	3 cut(s)	355, 1408, 2156
MspI	CCGG	8 cut(s)	486, 534, 591, 649, 665, 689, 794, 1841
MspR9I	CCNGG	5 cut(s)	559, 923, 1235, 1421, 1842
Mva1269I	GAATGC	3 cut(s)	724, 1189, 2083
MvaI	CCWGG	4 cut(s)	559, 923, 1235, 1421
MvnI	CGCG	7 cut(s)	130, 388, 390, 409, 640, 645, 685
NaeI	GCCGGC	1 cut(s)	487
NciI	CCSGG	1 cut(s)	1842
NcoI	CCATGG	1 cut(s)	1308
NdeII	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
NgoMIV	GCCGGC	1 cut(s)	485
NlaIII	CATG	6 cut(s)	526, 726, 1265, 1312, 1705, 1778
NlaIV	GGNNCC	3 cut(s)	787, 1232, 1306
NmeAIII	GCCGAG	3 cut(s)	435, 685, 814
NmuCI	GTSAC	2 cut(s)	634, 1264
NsiI	ATGCAT	2 cut(s)	724, 2085
NspI	RCATGY	2 cut(s)	726, 1705
NspV	TTCGAA	2 cut(s)	1040, 1650
PaeR7I	CTCGAG	2 cut(s)	1147, 1246
PauI	GCGCGC	1 cut(s)	388
PceI	AGGCCT	1 cut(s)	557
PciI	ACATGT	1 cut(s)	1701
PciSI	GCTCTTC	1 cut(s)	629
PcsI	WCGNNNNNNNCGW	1 cut(s)	606
PctI	GAATGC	3 cut(s)	724, 1189, 2083
PdiI	GCCGGC	1 cut(s)	487
PdmI	GAANNNNTTC	1 cut(s)	761
PfeI	GAWTC	5 cut(s)	292, 1012, 1742, 1916, 2038
PleI	GAGTC	3 cut(s)	674, 787, 1257
PpsI	GAGTC	3 cut(s)	674, 787, 1257
PscI	ACATGT	1 cut(s)	1701
Psp6I	CCWGG	4 cut(s)	557, 921, 1233, 1419
PspFI	CCCAGC	3 cut(s)	172, 351, 1187
PspGI	CCWGG	4 cut(s)	557, 921, 1233, 1419
PspN4I	GGNNCC	3 cut(s)	787, 1232, 1306
PspPI	GGNCC	2 cut(s)	602, 1312
PspXI	VCTCGAGB	2 cut(s)	1147, 1246
PstI	CTGCAG	2 cut(s)	964, 1413
PstNI	CAGNNNCTG	1 cut(s)	1408
PsuI	RGATCY	2 cut(s)	1921, 2219
PteI	GCGCGC	1 cut(s)	388
PvuII	CAGCTG	3 cut(s)	355, 1408, 2156
RigI	GGCCGGCC	1 cut(s)	489
RsaI	GTAC	1 cut(s)	1885
RsaNI	GTAC	1 cut(s)	1884
RseI	CAYNNNNRTG	3 cut(s)	1236, 1598, 1788
SapI	GCTCTTC	1 cut(s)	629
SaqAI	TTAA	1 cut(s)	1662
Sau3AI	GATC	7 cut(s)	819, 891, 1494, 1581, 1666, 1921, 2219
Sau96I	GGNCC	2 cut(s)	602, 1312
SchI	GAGTC	3 cut(s)	674, 788, 1258
ScrFI	CCNGG	5 cut(s)	559, 923, 1235, 1421, 1842
SduI	GDGCHC	2 cut(s)	1321, 1534
SfaNI	GCATC	3 cut(s)	1203, 1444, 2070
SfcI	CTRYAG	2 cut(s)	960, 1409
Sfr274I	CTCGAG	2 cut(s)	1147, 1246
SfuI	TTCGAA	2 cut(s)	1040, 1650
SinI	GGWCC	1 cut(s)	1312
SlaI	CTCGAG	2 cut(s)	1147, 1246
SmiMI	CAYNNNNRTG	3 cut(s)	1236, 1598, 1788
SmlI	CTYRAG	5 cut(s)	617, 1147, 1217, 1246, 1388
SmoI	CTYRAG	5 cut(s)	617, 1147, 1217, 1246, 1388
SpeI	ACTAGT	1 cut(s)	2099
SseBI	AGGCCT	1 cut(s)	557
SspMI	CTAG	7 cut(s)	834, 1047, 1101, 1331, 1811, 2100, 2121
StuI	AGGCCT	1 cut(s)	557
StyD4I	CCNGG	5 cut(s)	557, 921, 1233, 1419, 1840
StyI	CCWWGG	2 cut(s)	480, 1308
TaaI	ACNGT	5 cut(s)	898, 1207, 1279, 1316, 1523
TaiI	ACGT	2 cut(s)	552, 1376
TaqI	TCGA	9 cut(s)	441, 765, 1040, 1148, 1247, 1497, 1650, 1710, 2087
TatI	WGTACW	1 cut(s)	1883
TfiI	GAWTC	5 cut(s)	292, 1012, 1742, 1916, 2038
Tru1I	TTAA	1 cut(s)	1662
Tru9I	TTAA	1 cut(s)	1662
TscAI	CASTG	4 cut(s)	903, 1170, 1230, 1528
TseFI	GTSAC	2 cut(s)	634, 1264
TseI	GCWGC	6 cut(s)	584, 710, 1405, 1408, 1985, 2153
Tsp45I	GTSAC	2 cut(s)	634, 1264
TspDTI	ATGAA	9 cut(s)	17, 252, 1184, 1196, 1563, 1616, 1781, 1881, 1959
TspGWI	ACGGA	1 cut(s)	622
TspRI	CASTG	4 cut(s)	903, 1170, 1230, 1528
VpaK11BI	GGWCC	1 cut(s)	1312
XagI	CCTNNNNNAGG	2 cut(s)	780, 1944
XapI	RAATTY	4 cut(s)	916, 1036, 1042, 1087
XceI	RCATGY	2 cut(s)	726, 1705
XhoI	CTCGAG	2 cut(s)	1147, 1246
XmiI	GTMKAC	1 cut(s)	1136
XmnI	GAANNNNTTC	1 cut(s)	761
XspI	CTAG	7 cut(s)	834, 1047, 1101, 1331, 1811, 2100, 2121
Zsp2I	ATGCAT	2 cut(s)	724, 2085

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.