RLG00000025101

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009745
Physical Location & Seq
Forward (+)
41985380 .. 41986164
785 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025101

Sequence Viewer

Length: 447 bp
ATGCCCGACGTGGTGGCCCACACCGCCGTCATCGAGGCCTACGCCGCCGCCGGAAAGCCCAAGGACGCTCTCAAGGTGTACATGCGGATGTTGGCGTGTGGGGTTGCACCAAATACCTACTCTTATAGCGTGCTCATCAAGGCACTGGCTGCCGACGCCAGTTTCGTTGGGGACGCGAAGAAGTATCTGATGGAGATGATGGGGAAGGGAATGCGGCCCAATGCCGGGACGTACGTGGCGGTGTTTGAGGCGTTTGCGGCCGAGGAGAGGGTGGAGGAGGGGAGGGAGTTGCTGGAGGAGATGAAAGGGAAGGGGTTTGTGGCGGACGAGAAGGCGGTGAGGGAGGTGCTCAAGGGTAGAAGAGGAGCTGTGTACTTACAAACTACTGCTTCAACTGTTAGCTTAACTTTCATCGGCGTCTCCATTTTGGCCGGCTCTCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 Ribosomal_L15e

Protein Analysis

149

Amino Acids

15.83

Weight (kDa)

7.78

Isoelectric Point (pI)

26.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 2 - 50 1.6e-13 PPR repeat family
PPR_3 PF13812 2 - 49 3.4e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 7 - 67 3.3e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 21 - 118 3.3e-08 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 176
AciI CCGC 9 cut(s) 24, 45, 48, 85, 214, 239, 257, 323, 335
AcoI YGGCCR 2 cut(s) 258, 429
AcyI GRCGYC 2 cut(s) 156, 417
AfaI GTAC 3 cut(s) 80, 233, 374
AfiI CCNNNNNNNGG 3 cut(s) 224, 225, 267
AgsI TTSAA 1 cut(s) 393
AjiI CACGTC 1 cut(s) 10
AluBI AGCT 2 cut(s) 368, 402
AluI AGCT 2 cut(s) 368, 402
Alw21I GWGCWC 2 cut(s) 135, 351
Alw26I GTCTC 1 cut(s) 424
AoxI GGCC 5 cut(s) 15, 36, 215, 258, 429
ApeKI GCWGC 1 cut(s) 149
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspS9I GGNCC 2 cut(s) 16, 216
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 349
Bbv12I GWGCWC 2 cut(s) 135, 351
BbvI GCAGC 1 cut(s) 136
BccI CCATC 2 cut(s) 184, 193
BceAI ACGGC 1 cut(s) 11
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 424
BisI GCNGC 5 cut(s) 45, 48, 150, 215, 258
BlsI GCNGC 5 cut(s) 46, 49, 151, 216, 259
Bme1390I CCNGG 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 10
BmgT120I GGNCC 2 cut(s) 16, 216
BmrFI CCNGG 1 cut(s) 226
BpmI CTGGAG 1 cut(s) 314
BpuEI CTTGAG 2 cut(s) 56, 335
BpuMI CCSGG 1 cut(s) 226
BsaAI YACGTR 1 cut(s) 235
BsaHI GRCGYC 2 cut(s) 156, 417
BsaJI CCNNGG 2 cut(s) 60, 261
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 225, 267
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 150, 159
BseDI CCNNGG 2 cut(s) 60, 261
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 224, 225, 267
BseNI ACTGG 2 cut(s) 150, 159
BseRI GAGGAG 4 cut(s) 278, 290, 311, 378
BseX3I CGGCCG 1 cut(s) 258
BseXI GCAGC 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 261
BshFI GGCC 5 cut(s) 17, 38, 217, 260, 431
BsiEI CGRYCG 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 135, 351
BsiSI CCGG 3 cut(s) 51, 225, 432
BsiWI CGTACG 1 cut(s) 231
BslFI GGGAC 2 cut(s) 185, 241
BslI CCNNNNNNNGG 3 cut(s) 224, 225, 267
BsmAI GTCTC 1 cut(s) 424
BsmBI CGTCTC 1 cut(s) 424
BsmFI GGGAC 2 cut(s) 185, 241
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 5 cut(s) 17, 38, 217, 260, 431
Bsp1286I GDGCHC 2 cut(s) 135, 351
Bsp1407I TGTACA 1 cut(s) 78
BspACI CCGC 9 cut(s) 24, 45, 48, 85, 214, 239, 257, 323, 335
BspANI GGCC 5 cut(s) 17, 38, 217, 260, 431
BspFNI CGCG 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 431
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 2 cut(s) 150, 159
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 60, 261
BssNI GRCGYC 2 cut(s) 156, 417
BssT1I CCWWGG 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 2 cut(s) 355, 444
BstACI GRCGYC 2 cut(s) 156, 417
BstAPI GCANNNNNTGC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 78
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 131, 433
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 176
BstMAI GTCTC 1 cut(s) 424
BstMCI CGRYCG 1 cut(s) 261
BstMWI GCNNNNNNNGC 5 cut(s) 23, 44, 149, 155, 257
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 1 cut(s) 224
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 136
BstZI CGGCCG 1 cut(s) 258
BsuRI GGCC 5 cut(s) 17, 38, 217, 260, 431
BtrI CACGTC 1 cut(s) 10
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 131, 433
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 2 cut(s) 16, 216
CseI GACGC 4 cut(s) 74, 164, 182, 406
Csp6I GTAC 3 cut(s) 79, 232, 373
CviAII CATG 1 cut(s) 82
CviQI GTAC 3 cut(s) 79, 232, 373
EaeI YGGCCR 2 cut(s) 258, 429
EagI CGGCCG 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 355, 444
EarI CTCTTC 2 cut(s) 355, 444
EciI GGCGGA 1 cut(s) 338
EclXI CGGCCG 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 60
Eco147I AGGCCT 1 cut(s) 38
Eco52I CGGCCG 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
Esp3I CGTCTC 1 cut(s) 424
FaeI CATG 1 cut(s) 85
FaiI YATR 2 cut(s) 83, 126
FaqI GGGAC 2 cut(s) 185, 241
FatI CATG 1 cut(s) 81
Fnu4HI GCNGC 5 cut(s) 45, 48, 150, 215, 258
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 5 cut(s) 45, 48, 150, 215, 258
GluI GCNGC 5 cut(s) 45, 48, 150, 215, 258
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 5 cut(s) 17, 38, 217, 260, 431
HapII CCGG 3 cut(s) 51, 225, 432
HgaI GACGC 4 cut(s) 74, 164, 182, 406
Hin1I GRCGYC 2 cut(s) 156, 417
Hin1II CATG 1 cut(s) 85
HpaII CCGG 3 cut(s) 51, 225, 432
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 2 cut(s) 79, 373
Hpy188I TCNGA 1 cut(s) 189
Hpy8I GTNNAC 2 cut(s) 79, 373
Hpy99I CGWCG 2 cut(s) 11, 158
HpyAV CCTTC 3 cut(s) 199, 304, 325
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 3 cut(s) 9, 230, 234
HpyCH4V TGCA 1 cut(s) 107
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 44, 149, 155, 257
HpySE526I ACGT 3 cut(s) 9, 230, 234
Hsp92I GRCGYC 2 cut(s) 156, 417
Hsp92II CATG 1 cut(s) 85
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 365
LpnPI CCDG 5 cut(s) 64, 131, 172, 238, 278
Lsp1109I GCAGC 1 cut(s) 136
MaeII ACGT 3 cut(s) 9, 230, 234
MboII GAAGA 3 cut(s) 190, 372, 431
MhlI GDGCHC 2 cut(s) 135, 351
MroNI GCCGGC 1 cut(s) 431
MseI TTAA 2 cut(s) 404, 445
MslI CAYNNNNRTG 1 cut(s) 86
MspI CCGG 3 cut(s) 51, 225, 432
MspR9I CCNGG 1 cut(s) 226
Mva1269I GAATGC 1 cut(s) 216
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 5 cut(s) 23, 44, 149, 155, 257
NaeI GCCGGC 1 cut(s) 433
NciI CCSGG 1 cut(s) 226
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 1 cut(s) 85
NmeAIII GCCGAG 1 cut(s) 286
NspI RCATGY 1 cut(s) 85
PceI AGGCCT 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 216
PdiI GCCGGC 1 cut(s) 433
Pfl23II CGTACG 1 cut(s) 231
PkrI GCNGC 5 cut(s) 46, 49, 151, 216, 259
Ppu21I YACGTR 1 cut(s) 235
PspLI CGTACG 1 cut(s) 231
PspPI GGNCC 2 cut(s) 16, 216
RsaI GTAC 3 cut(s) 80, 233, 374
RsaNI GTAC 3 cut(s) 79, 232, 373
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 2 cut(s) 404, 445
SatI GCNGC 5 cut(s) 45, 48, 150, 215, 258
Sau96I GGNCC 2 cut(s) 16, 216
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 2 cut(s) 135, 351
SetI ASST 8 cut(s) 12, 78, 119, 233, 237, 348, 370, 404
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 71, 350
SmoI CTYRAG 2 cut(s) 71, 350
SseBI AGGCCT 1 cut(s) 38
SsiI CCGC 9 cut(s) 24, 45, 48, 85, 214, 239, 257, 323, 335
StuI AGGCCT 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 3 cut(s) 12, 233, 237
TaqI TCGA 1 cut(s) 33
TatI WGTACW 2 cut(s) 78, 372
TauI GCSGC 4 cut(s) 47, 50, 217, 260
Tru1I TTAA 2 cut(s) 404, 445
Tru9I TTAA 2 cut(s) 404, 445
TscAI CASTG 1 cut(s) 150
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 2 cut(s) 317, 400
TspRI CASTG 1 cut(s) 150
XceI RCATGY 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.