Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0008143

Physical Location & Seq

Genomic Coordinates Reverse (-)

46672235 .. 46674922

Gene Structure 2688 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025558

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2688 bp

ATGGAGAAGATGACATTGGATTCGAAGGAATATGAGTTGAAGAAGAACAGTATGAGAGAGATGTACGAGAGCTTGCACTCCCAAGCATCTTCCATGCTAGTCTTCACCCTTCAGTGGAAGGAACTGGAGGGACGCTTAGATTCGATCCGGGATGCGACCCAGACCCGACTCGAAGAGCTCCATGAACGAGAGCAGCAGTTGGATGCCAAAGAGTTAAAGATGGTGTCCAAAGCAAATGAGTTCCAGGAACGAGAGAAGCAGTTGGAGGCCAAAGAGTTAAAGATCCAGTCCGAAGTCAATGAGGCGTTTGGACTCGAAAAGCTGATTAAGGAGAAACAACAAGAGGCTATAGACAGCAAGAACTACTTGAATTCGCTCCAGTTATCGATCAAAGAGCATACCGAATCAGAGAAAGAGCTTGGAGCCAAGGAAAGACAAATAGAGAGACGAATTCAAGAATTGAATGGGGTTGAGAGGAATATAGAAGAGAAGTTGAAGAAATCTCGTGAGGAACTGAATCAGATTCAGAGGGCGATTAAGGAGCAGGGTGTAGAGTTTGATTTGAAAGAAAAGGAAATTAAAGTGAAAGAGGAGAAATTGAATGGGATTGAGAAGAGTATCGAACAGAAGTTGAAATTGGCAAATGAGGAGGAGAAGAAAGCATTTGATCTGAAACAAGAGCGTGCTAAAGAAGAGCGGAAACTGAGAGAGGAGAGACTGAAAGAGGAGGAGAAGTTGAAAGAGCATAAGAAATCAGTAGTGGAAACCTCTAAGACACTTAAGTCAAGAGAGCAAATAAAGAGGGAACTAGAACTGAAAGTGAAAGATCATTGCTCGCGAAAGAAATCAATGGAAGAGTGGTCCTCTAATCTCGAACGTAAAGAGAGAGAATTAGAATTGAAAGAGAAGCAAGTTTTGTCAAAAGCTGAGGAACTTGACTCGCTTAATAAAAGGACCCTTAATGAGTTTCAGTTGATGGAAAAGGAACTTAGTTCAGTCGAATGTAAGATACAACAACGTGAAAAGCAATTGGAAGCAGACTCCCACCGACTTCAAGTGAAAGAAAGGCAACTTGAACTGCAAAGTGAAACAATGGAAAAGTTGCAGCAAGAATGCAAAAAGCAACTTGAAGCGAGAGAGAGACAACTTGAAGTAAAAAGTGATAAAATGGAAAAATTGCATCAGGAACGCGCAAAAAAACTTGAAGCCAAAGGGAGACAACTTGAAGAGCAGAGCAAAGAGCTTGAATTGACAAAGAAGCAATTTGGTTGTCAAATAAAAGTAAAAAGTGAGCCACTGGAAAATACCCCAGCTGCTAATAATGCCATTGTTTCTTCATCTGCAGGTGATCATTCCAAAATCAATATCAGGGATGGTAGAGGCTTGCAACTATTCATGTATGAGCATATGAAGAGAAATGATTCAGTGAGCAAAGCAATCTCAGCTGTTCTTCAACAAGAGTCATTAGAGCCAGCAAAATTGGTTTTGGATGCAATGGAAGGGTTTCACCCTTCAAATTCGACTGTGGACAATAGGGAGTTTGATTTGGTAATTATTAGAAGGACATGTATTCTTTTGTTGAAAGAGTTAAAGAGAGTCTCGGCGCAAATTAATCCTCAGCTGAGAGGACAAGCAATGAAGTTGGCTGGTGATTGGAAGGCTAAGATGACGGTGCGGACTGAGAATTGGTTAGAGGTTTTGGGATTTTTGCGGCTTGTTACTACATATGAATTGACCTCTGGCTATGATGCAAATGAGCTTCAAGGTCTTCTTCATATAGTAGCTCAACATGAACAAGCAACTGAATTATCCCGAGCCCTTGGTACCACAGATAAGGCAGCTGCCAACAACATTAGTACTGTTGCATCTGTTAAAAATGAGAAAGCAGACTCGCCTGCACAAACAACTTTTTCTTCTCCAAATCTTCAACCAACTGCCAAGAACAAAATTATTGTTGCTCCTGTCACAACTAAGAAAGCTCCTGATGTTGAAACTAAGAATGCGAAAAGTTCTGCAGCCGCAATCTTTTCTTCTCCTAATCTTCAACCAGCTACCACTGTCCCAGCTAATGCTATTATTGCTATTCAGAATTTTATTAGAGAGAAGAAACTGGTGAAGGCTTATGGACGCATTCACAAATTCAAGTTATTAGACAAGTTCCCTCCAGCTCAAGTCTTAAAAGAATATGTTGAGAAGGCCATGAAGTGCATGGAAAATAGCGAGAGAAATGACATGCTCCATCAACAGGATCAGGTTGTAGACGAAGGAATATCTGATCTACGACGTGCTATTGAATGCATCAAATATCACAACCTTGAGTCGGAATACCCTTCTGAGAGCATTGAGAAACAAATAGTTGTGCTACAGAAATTTATGGAGGATCGGAAAAACCAAAATCAGAAAAAACGCTTATGCAGCACAATTGATTCCAGCTTTCAACAGCAGGAACATAAATTTCAGCGGACATCCTTATCAGCGGGGAGACCATATGGATTGCACCCTGTCTATCCGAATCCAGGTTCATCATTTTACAATGGACAGTTTAGTATTCCTTCCAACAATCCCGTGCAGTTTAGCATGCCTGCCAATGGCGTTAATTATGAGTTCCCTAGAATATATCCATACCCTCCATACCCTCGCCCTCTTGTATATAGACACCCTGGGTATGGCCTTCAAAAGCCATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

896

Amino Acids

103.72

Weight (kDa)

8.72

Isoelectric Point (pI)

51.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	458 - 612	1.9e-32	Frigida-like protein
Frigida	PF07899	695 - 798	3.4e-18	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0008143)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04251
rosa_chinensis	RchiOBHm_Chr3g0452961
rosa_laevigata	RLG00000025558 RLG00000035001 RLG00000035002
rosa_multiflora	Rmu_sc0008989.1_g000006
rosa_roxburghii	Rroxscaffold_1G00024630 Rroxscaffold_6G00429960 Rroxscaffold_6G00430020
rosa_rugosa	Rorug02G0643800 Rorug02G0643800 Rorug02G0643800 Rorug02G0643800 Rorug05G0294400
rosa_samantha	Rh3AG046900 Rh3BG049300 Rh3CG048000 Rh3DG048900 Rh5AG364000 Rh5BG375900 Rh5CG398400 Rh5DG389100
rosa_wichuraiana	Rw3G003680 Rw5G034230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1334
AasI	GACNNNNNNGTC	1 cut(s)	781
Acc36I	ACCTGC	1 cut(s)	1334
Acc65I	GGTACC	1 cut(s)	1823
AccB1I	GGYRCC	1 cut(s)	1823
AccBSI	CCGCTC	1 cut(s)	697
AccI	GTMKAC	1 cut(s)	2259
AccII	CGCG	2 cut(s)	838, 1191
AciI	CCGC	6 cut(s)	697, 1675, 1711, 2019, 2461, 2477
AclWI	GGATC	4 cut(s)	139, 277, 2256, 2388
AcsI	RAATTY	7 cut(s)	370, 450, 1516, 2089, 2138, 2369, 2453
AcuI	CTGAAG	1 cut(s)	95
AfaI	GTAC	3 cut(s)	65, 1825, 1858
AfiI	CCNNNNNNNGG	6 cut(s)	114, 2245, 2320, 2516, 2588, 2666
AflII	CTTAAG	1 cut(s)	779
AflIII	ACRYGT	1 cut(s)	1565
AjiI	CACGTC	1 cut(s)	2285
AjnI	CCWGG	3 cut(s)	243, 2515, 2659
AjuI	GAANNNNNNNTTGG	3 cut(s)	31, 620, 652
Alw21I	GWGCWC	1 cut(s)	180
Alw26I	GTCTC	6 cut(s)	439, 709, 1135, 1210, 1603, 2476
AlwI	GGATC	4 cut(s)	139, 277, 2256, 2388
Ama87I	CYCGRG	1 cut(s)	1812
AoxI	GGCC	3 cut(s)	267, 2196, 2668
ApeKI	GCWGC	7 cut(s)	193, 1105, 1313, 1838, 1841, 2015, 2415
ApoI	RAATTY	7 cut(s)	370, 450, 1516, 2089, 2138, 2369, 2453
ArsI	GACNNNNNNTTYG	1 cut(s)	36
AseI	ATTAAT	1 cut(s)	1611
Asp700I	GAANNNNTTC	2 cut(s)	1420, 1503
Asp718I	GGTACC	1 cut(s)	1823
AspLEI	GCGC	2 cut(s)	1193, 1606
AspS9I	GGNCC	2 cut(s)	861, 954
AsuC2I	CCSGG	1 cut(s)	149
AsuHPI	GGTGA	5 cut(s)	97, 1358, 1499, 1661, 2125
AsuII	TTCGAA	1 cut(s)	23
AvaI	CYCGRG	1 cut(s)	1812
AvaII	GGWCC	2 cut(s)	861, 954
BanI	GGYRCC	1 cut(s)	1823
BanII	GRGCYC	2 cut(s)	180, 1819
BauI	CACGAG	1 cut(s)	504
BbsI	GAAGAC	2 cut(s)	94, 1760
Bbv12I	GWGCWC	1 cut(s)	180
BbvCI	CCTCAGC	2 cut(s)	927, 1617
BbvI	GCAGC	7 cut(s)	205, 1117, 1300, 1828, 1850, 2027, 2427
BccI	CCATC	4 cut(s)	214, 970, 1367, 2247
BciT130I	CCWGG	3 cut(s)	245, 2517, 2661
BclI	TGATCA	1 cut(s)	1348
BcnI	CCSGG	1 cut(s)	149
BcoDI	GTCTC	6 cut(s)	439, 709, 1135, 1210, 1603, 2476
BfaI	CTAG	3 cut(s)	98, 809, 2610
BfmI	CTRYAG	4 cut(s)	348, 1341, 2013, 2363
BfrI	CTTAAG	1 cut(s)	779
BfuAI	ACCTGC	1 cut(s)	1334
BisI	GCNGC	9 cut(s)	194, 1106, 1314, 1712, 1839, 1842, 2016, 2019, 2416
BlsI	GCNGC	9 cut(s)	195, 1107, 1315, 1713, 1840, 1843, 2017, 2020, 2417
BmcAI	AGTACT	1 cut(s)	1858
Bme1390I	CCNGG	4 cut(s)	149, 245, 2517, 2661
Bme18I	GGWCC	2 cut(s)	861, 954
BmeT110I	CYCGRG	1 cut(s)	1812
BmgBI	CACGTC	1 cut(s)	2285
BmgT120I	GGNCC	2 cut(s)	861, 954
BmiI	GGNNCC	3 cut(s)	424, 956, 1825
BmrFI	CCNGG	4 cut(s)	149, 245, 2517, 2661
BmsI	GCATC	8 cut(s)	95, 142, 193, 1189, 1480, 1738, 1874, 2307
BpiI	GAAGAC	2 cut(s)	94, 1760
BplI	GAGNNNNNCTC	2 cut(s)	848, 880
BpmI	CTGGAG	3 cut(s)	146, 362, 2148
Bpu10I	CCTNAGC	2 cut(s)	927, 1617
Bpu14I	TTCGAA	1 cut(s)	23
BpuEI	CTTGAG	2 cut(s)	2154, 2336
BpuMI	CCSGG	1 cut(s)	149
Bsa29I	ATCGAT	1 cut(s)	386
BsaI	GGTCTC	1 cut(s)	2476
BsaJI	CCNNGG	4 cut(s)	426, 1819, 2659, 2660
BsaXI	ACNNNNNCTCC	2 cut(s)	719, 749
Bsc4I	CCNNNNNNNGG	6 cut(s)	114, 2245, 2320, 2516, 2588, 2666
Bse1I	ACTGG	5 cut(s)	129, 286, 379, 1302, 2115
Bse3DI	GCAATG	3 cut(s)	829, 1500, 1641
BseBI	CCWGG	3 cut(s)	245, 2517, 2661
BseCI	ATCGAT	1 cut(s)	386
BseDI	CCNNGG	4 cut(s)	426, 1819, 2659, 2660
BseGI	GGATG	5 cut(s)	157, 208, 1378, 1495, 2465
BseLI	CCNNNNNNNGG	6 cut(s)	114, 2245, 2320, 2516, 2588, 2666
BseMI	GCAATG	3 cut(s)	829, 1500, 1641
BseMII	CTCAG	7 cut(s)	695, 918, 1455, 1613, 1631, 1671, 2325
BseNI	ACTGG	5 cut(s)	129, 286, 379, 1302, 2115
BseRI	GAGGAG	6 cut(s)	605, 662, 665, 725, 740, 743
BseXI	GCAGC	7 cut(s)	205, 1117, 1300, 1828, 1850, 2027, 2427
BseYI	CCCAGC	2 cut(s)	1309, 2062
BsgI	GTGCAG	2 cut(s)	1881, 2588
Bsh1236I	CGCG	2 cut(s)	838, 1191
BshFI	GGCC	3 cut(s)	269, 2198, 2670
BshNI	GGYRCC	1 cut(s)	1823
BshVI	ATCGAT	1 cut(s)	386
BsiHKAI	GWGCWC	1 cut(s)	180
BsiHKCI	CYCGRG	1 cut(s)	1812
BsiSI	CCGG	1 cut(s)	148
BslFI	GGGAC	2 cut(s)	144, 2045
BslI	CCNNNNNNNGG	6 cut(s)	114, 2245, 2320, 2516, 2588, 2666
BsmAI	GTCTC	6 cut(s)	439, 709, 1135, 1210, 1603, 2476
BsmBI	CGTCTC	1 cut(s)	439
BsmFI	GGGAC	2 cut(s)	144, 2045
BsmI	GAATGC	4 cut(s)	1118, 2005, 2130, 2300
BsnI	GGCC	3 cut(s)	269, 2198, 2670
Bso31I	GGTCTC	1 cut(s)	2476
BsoBI	CYCGRG	1 cut(s)	1812
Bsp119I	TTCGAA	1 cut(s)	23
Bsp1286I	GDGCHC	2 cut(s)	180, 1819
Bsp143I	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
Bsp68I	TCGCGA	1 cut(s)	838
BspACI	CCGC	6 cut(s)	697, 1675, 1711, 2019, 2461, 2477
BspANI	GGCC	3 cut(s)	269, 2198, 2670
BspCNI	CTCAG	7 cut(s)	696, 919, 1454, 1614, 1630, 1672, 2326
BspDI	ATCGAT	1 cut(s)	386
BspFNI	CGCG	2 cut(s)	838, 1191
BspLI	GGNNCC	3 cut(s)	424, 956, 1825
BspMAI	CTGCAG	2 cut(s)	1345, 2017
BspMI	ACCTGC	1 cut(s)	1334
BspPI	GGATC	4 cut(s)	139, 277, 2256, 2388
BspQI	GCTCTTC	3 cut(s)	168, 687, 1221
BspT104I	TTCGAA	1 cut(s)	23
BspT107I	GGYRCC	1 cut(s)	1823
BspTI	CTTAAG	1 cut(s)	779
BspTNI	GGTCTC	1 cut(s)	2476
BsrBI	CCGCTC	1 cut(s)	697
BsrDI	GCAATG	3 cut(s)	829, 1500, 1641
BsrI	ACTGG	5 cut(s)	129, 286, 379, 1302, 2115
BssECI	CCNNGG	4 cut(s)	426, 1819, 2659, 2660
BssMI	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
BssSI	CACGAG	1 cut(s)	504
BssT1I	CCWWGG	2 cut(s)	426, 1819
Bst2BI	CACGAG	1 cut(s)	504
Bst2UI	CCWGG	3 cut(s)	245, 2517, 2661
Bst4CI	ACNGT	6 cut(s)	50, 1525, 1672, 1861, 2059, 2541
Bst6I	CTCTTC	7 cut(s)	168, 480, 608, 687, 849, 1221, 1406
BstAFI	CTTAAG	1 cut(s)	779
BstBI	TTCGAA	1 cut(s)	23
BstC8I	GCNNGC	8 cut(s)	74, 684, 836, 1385, 1473, 1896, 2579, 2583
BstF5I	GGATG	5 cut(s)	157, 208, 1378, 1495, 2465
BstFNI	CGCG	2 cut(s)	838, 1191
BstHHI	GCGC	2 cut(s)	1193, 1606
BstKTI	GATC	9 cut(s)	147, 285, 390, 670, 829, 1351, 2251, 2278, 2383
BstMAI	GTCTC	6 cut(s)	439, 709, 1135, 1210, 1603, 2476
BstMBI	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
BstMWI	GCNNNNNNNGC	4 cut(s)	1322, 1442, 2078, 2415
BstNI	CCWGG	3 cut(s)	245, 2517, 2661
BstNSI	RCATGY	3 cut(s)	1569, 2236, 2581
BstSCI	CCNGG	4 cut(s)	147, 243, 2515, 2659
BstSFI	CTRYAG	4 cut(s)	348, 1341, 2013, 2363
BstUI	CGCG	2 cut(s)	838, 1191
BstV1I	GCAGC	7 cut(s)	205, 1117, 1300, 1828, 1850, 2027, 2427
BstV2I	GAAGAC	2 cut(s)	94, 1760
BstX2I	RGATCY	1 cut(s)	282
BstYI	RGATCY	1 cut(s)	282
Bsu15I	ATCGAT	1 cut(s)	386
BsuRI	GGCC	3 cut(s)	269, 2198, 2670
BsuTUI	ATCGAT	1 cut(s)	386
BtrI	CACGTC	1 cut(s)	2285
BtsCI	GGATG	5 cut(s)	157, 208, 1378, 1495, 2465
BtsIMutI	CAGTG	4 cut(s)	119, 1295, 1431, 2055
BtuMI	TCGCGA	1 cut(s)	838
BveI	ACCTGC	1 cut(s)	1334
Cac8I	GCNNGC	8 cut(s)	74, 684, 836, 1385, 1473, 1896, 2579, 2583
CfoI	GCGC	2 cut(s)	1193, 1606
Cfr13I	GGNCC	2 cut(s)	861, 954
ClaI	ATCGAT	1 cut(s)	386
CseI	GACGC	2 cut(s)	141, 2136
Csp6I	GTAC	3 cut(s)	64, 1824, 1857
CviAII	CATG	9 cut(s)	94, 182, 1396, 1566, 1790, 2200, 2209, 2233, 2578
CviQI	GTAC	3 cut(s)	64, 1824, 1857
DpnI	GATC	9 cut(s)	146, 284, 389, 669, 828, 1350, 2250, 2277, 2382
DpnII	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
DrdI	GACNNNNNNGTC	1 cut(s)	781
DseDI	GACNNNNNNGTC	1 cut(s)	781
Eam1104I	CTCTTC	7 cut(s)	168, 480, 608, 687, 849, 1221, 1406
EarI	CTCTTC	7 cut(s)	168, 480, 608, 687, 849, 1221, 1406
Ecl136II	GAGCTC	1 cut(s)	178
Eco130I	CCWWGG	2 cut(s)	426, 1819
Eco24I	GRGCYC	2 cut(s)	180, 1819
Eco31I	GGTCTC	1 cut(s)	2476
Eco47I	GGWCC	2 cut(s)	861, 954
Eco53kI	GAGCTC	1 cut(s)	178
Eco57I	CTGAAG	1 cut(s)	95
Eco88I	CYCGRG	1 cut(s)	1812
EcoICRI	GAGCTC	1 cut(s)	178
EcoO109I	RGGNCCY	1 cut(s)	954
EcoRI	GAATTC	2 cut(s)	370, 450
EcoRII	CCWGG	3 cut(s)	243, 2515, 2659
EcoT14I	CCWWGG	2 cut(s)	426, 1819
EcoT22I	ATGCAT	1 cut(s)	2300
EcoT38I	GRGCYC	2 cut(s)	180, 1819
ErhI	CCWWGG	2 cut(s)	426, 1819
Esp3I	CGTCTC	1 cut(s)	439
FaeI	CATG	9 cut(s)	97, 185, 1399, 1569, 1793, 2203, 2212, 2236, 2581
FalI	AAGNNNNNCTT	4 cut(s)	350, 382, 1755, 1787
FaqI	GGGAC	2 cut(s)	144, 2045
FatI	CATG	9 cut(s)	93, 181, 1395, 1565, 1789, 2199, 2208, 2232, 2577
FauI	CCCGC	1 cut(s)	2470
FauNDI	CATATG	3 cut(s)	1407, 1726, 2488
FbaI	TGATCA	1 cut(s)	1348
FblI	GTMKAC	1 cut(s)	2259
Fnu4HI	GCNGC	9 cut(s)	194, 1106, 1314, 1712, 1839, 1842, 2016, 2019, 2416
FokI	GGATG	5 cut(s)	164, 215, 1385, 1502, 2452
FriOI	GRGCYC	2 cut(s)	180, 1819
Fsp4HI	GCNGC	9 cut(s)	194, 1106, 1314, 1712, 1839, 1842, 2016, 2019, 2416
FspBI	CTAG	3 cut(s)	98, 809, 2610
GlaI	GCGC	2 cut(s)	1192, 1605
GluI	GCNGC	9 cut(s)	194, 1106, 1314, 1712, 1839, 1842, 2016, 2019, 2416
GsaI	CCCAGC	2 cut(s)	1313, 2066
GsuI	CTGGAG	3 cut(s)	146, 362, 2148
HaeIII	GGCC	3 cut(s)	269, 2198, 2670
HapII	CCGG	1 cut(s)	148
HgaI	GACGC	2 cut(s)	141, 2136
HhaI	GCGC	2 cut(s)	1193, 1606
Hin1II	CATG	9 cut(s)	97, 185, 1399, 1569, 1793, 2203, 2212, 2236, 2581
Hin6I	GCGC	2 cut(s)	1191, 1604
HinP1I	GCGC	2 cut(s)	1191, 1604
HpaII	CCGG	1 cut(s)	148
HphI	GGTGA	5 cut(s)	97, 1358, 1499, 1661, 2125
Hpy166II	GTNNAC	2 cut(s)	1528, 2260
Hpy188III	TCNNGA	8 cut(s)	455, 506, 786, 837, 872, 1184, 1812, 1982
Hpy8I	GTNNAC	2 cut(s)	1528, 2260
Hpy99I	CGWCG	1 cut(s)	2286
HpyCH4III	ACNGT	6 cut(s)	50, 1525, 1672, 1861, 2059, 2541
HpyCH4IV	ACGT	3 cut(s)	877, 1018, 2284
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1322, 1442, 2078, 2415
HpySE526I	ACGT	3 cut(s)	877, 1018, 2284
Hsp92II	CATG	9 cut(s)	97, 185, 1399, 1569, 1793, 2203, 2212, 2236, 2581
HspAI	GCGC	2 cut(s)	1191, 1604
KpnI	GGTACC	1 cut(s)	1827
Ksp22I	TGATCA	1 cut(s)	1348
Kzo9I	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
LguI	GCTCTTC	3 cut(s)	168, 687, 1221
LmnI	GCTCC	7 cut(s)	183, 381, 422, 541, 1963, 1984, 2241
Lsp1109I	GCAGC	7 cut(s)	205, 1117, 1300, 1828, 1850, 2027, 2427
LweI	GCATC	8 cut(s)	95, 142, 193, 1189, 1480, 1738, 1874, 2307
MaeI	CTAG	3 cut(s)	98, 809, 2610
MaeII	ACGT	3 cut(s)	877, 1018, 2284
MaeIII	GTNAC	2 cut(s)	1717, 1963
MalI	GATC	9 cut(s)	146, 284, 389, 669, 828, 1350, 2250, 2277, 2382
MbiI	CCGCTC	1 cut(s)	697
MboI	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
MfeI	CAATTG	2 cut(s)	1028, 2421
MflI	RGATCY	1 cut(s)	282
MhlI	GDGCHC	2 cut(s)	180, 1819
MlyI	GAGTC	8 cut(s)	162, 306, 932, 1034, 1469, 1605, 1883, 2327
MmeI	TCCRAC	4 cut(s)	180, 243, 2301, 2580
Mph1103I	ATGCAT	1 cut(s)	2300
MroXI	GAANNNNTTC	2 cut(s)	1420, 1503
MspA1I	CMGCKG	6 cut(s)	1313, 1445, 1621, 1841, 2461, 2477
MspCI	CTTAAG	1 cut(s)	779
MspI	CCGG	1 cut(s)	148
MspR9I	CCNGG	4 cut(s)	149, 245, 2517, 2661
MunI	CAATTG	2 cut(s)	1028, 2421
Mva1269I	GAATGC	4 cut(s)	1118, 2005, 2130, 2300
MvaI	CCWGG	3 cut(s)	245, 2517, 2661
MvnI	CGCG	2 cut(s)	838, 1191
MwoI	GCNNNNNNNGC	4 cut(s)	1322, 1442, 2078, 2415
NciI	CCSGG	1 cut(s)	149
NdeI	CATATG	3 cut(s)	1407, 1726, 2488
NdeII	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
NlaIII	CATG	9 cut(s)	97, 185, 1399, 1569, 1793, 2203, 2212, 2236, 2581
NlaIV	GGNNCC	3 cut(s)	424, 956, 1825
NmeAIII	GCCGAG	1 cut(s)	1580
NmuCI	GTSAC	1 cut(s)	1963
NruI	TCGCGA	1 cut(s)	838
NsiI	ATGCAT	1 cut(s)	2300
NspI	RCATGY	3 cut(s)	1569, 2236, 2581
NspV	TTCGAA	1 cut(s)	23
PaeI	GCATGC	1 cut(s)	2581
PaqCI	CACCTGC	1 cut(s)	1334
PasI	CCCWGGG	1 cut(s)	2660
PciI	ACATGT	1 cut(s)	1565
PciSI	GCTCTTC	3 cut(s)	168, 687, 1221
PctI	GAATGC	4 cut(s)	1118, 2005, 2130, 2300
PdmI	GAANNNNTTC	2 cut(s)	1420, 1503
PfeI	GAWTC	8 cut(s)	20, 140, 404, 517, 523, 1421, 2426, 2512
PfoI	TCCNGGA	2 cut(s)	147, 243
PkrI	GCNGC	9 cut(s)	195, 1107, 1315, 1713, 1840, 1843, 2017, 2020, 2417
PleI	GAGTC	8 cut(s)	162, 306, 932, 1034, 1468, 1604, 1883, 2326
PpsI	GAGTC	8 cut(s)	162, 306, 932, 1034, 1468, 1604, 1883, 2326
PpuMI	RGGWCCY	1 cut(s)	954
PscI	ACATGT	1 cut(s)	1565
PshBI	ATTAAT	1 cut(s)	1611
Psp124BI	GAGCTC	1 cut(s)	180
Psp5II	RGGWCCY	1 cut(s)	954
Psp6I	CCWGG	3 cut(s)	243, 2515, 2659
PspFI	CCCAGC	2 cut(s)	1309, 2062
PspGI	CCWGG	3 cut(s)	243, 2515, 2659
PspN4I	GGNNCC	3 cut(s)	424, 956, 1825
PspPI	GGNCC	2 cut(s)	861, 954
PspPPI	RGGWCCY	1 cut(s)	954
PstI	CTGCAG	2 cut(s)	1345, 2017
PsuI	RGATCY	1 cut(s)	282
PvuII	CAGCTG	4 cut(s)	1313, 1445, 1621, 1841
RruI	TCGCGA	1 cut(s)	838
RsaI	GTAC	3 cut(s)	65, 1825, 1858
RsaNI	GTAC	3 cut(s)	64, 1824, 1857
SacI	GAGCTC	1 cut(s)	180
SapI	GCTCTTC	3 cut(s)	168, 687, 1221
SatI	GCNGC	9 cut(s)	194, 1106, 1314, 1712, 1839, 1842, 2016, 2019, 2416
Sau3AI	GATC	9 cut(s)	144, 282, 387, 667, 826, 1348, 2248, 2275, 2380
Sau96I	GGNCC	2 cut(s)	861, 954
ScaI	AGTACT	1 cut(s)	1858
SchI	GAGTC	8 cut(s)	162, 306, 932, 1034, 1469, 1605, 1883, 2327
ScrFI	CCNGG	4 cut(s)	149, 245, 2517, 2661
SduI	GDGCHC	2 cut(s)	180, 1819
SfaNI	GCATC	8 cut(s)	95, 142, 193, 1189, 1480, 1738, 1874, 2307
SfcI	CTRYAG	4 cut(s)	348, 1341, 2013, 2363
SfuI	TTCGAA	1 cut(s)	23
SinI	GGWCC	2 cut(s)	861, 954
SmlI	CTYRAG	3 cut(s)	779, 2169, 2315
SmoI	CTYRAG	3 cut(s)	779, 2169, 2315
SphI	GCATGC	1 cut(s)	2581
SsiI	CCGC	6 cut(s)	697, 1675, 1711, 2019, 2461, 2477
SspMI	CTAG	3 cut(s)	98, 809, 2610
SstI	GAGCTC	1 cut(s)	180
StyD4I	CCNGG	4 cut(s)	147, 243, 2515, 2659
StyI	CCWWGG	2 cut(s)	426, 1819
TaaI	ACNGT	6 cut(s)	50, 1525, 1672, 1861, 2059, 2541
TaiI	ACGT	3 cut(s)	880, 1021, 2287
TaqI	TCGA	9 cut(s)	23, 143, 171, 315, 386, 621, 873, 999, 1520
TatI	WGTACW	1 cut(s)	1856
TauI	GCSGC	2 cut(s)	1714, 2021
TfiI	GAWTC	8 cut(s)	20, 140, 404, 517, 523, 1421, 2426, 2512
TscAI	CASTG	4 cut(s)	119, 1302, 1431, 2062
TseFI	GTSAC	1 cut(s)	1963
TseI	GCWGC	7 cut(s)	193, 1105, 1313, 1838, 1841, 2015, 2415
Tsp45I	GTSAC	1 cut(s)	1963
TspRI	CASTG	4 cut(s)	119, 1302, 1431, 2062
Vha464I	CTTAAG	1 cut(s)	779
VpaK11BI	GGWCC	2 cut(s)	861, 954
VspI	ATTAAT	1 cut(s)	1611
XapI	RAATTY	7 cut(s)	370, 450, 1516, 2089, 2138, 2369, 2453
XceI	RCATGY	3 cut(s)	1569, 2236, 2581
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2206
XmiI	GTMKAC	1 cut(s)	2259
XmnI	GAANNNNTTC	2 cut(s)	1420, 1503
XspI	CTAG	3 cut(s)	98, 809, 2610
ZrmI	AGTACT	1 cut(s)	1858
Zsp2I	ATGCAT	1 cut(s)	2300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.