Rw5G034230

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008143
Physical Location & Seq
Reverse (-)
58881750 .. 58895501
13752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034230.1

Sequence Viewer

Length: 1437 bp
ATGGAGGAGATGACATTGGATTTGAAGGAATATGAGTTGAAGAAGAACAGTATGAGAAACGTGTGCGAGAGCTTGCACTCCCAAGCATCTTCCATGCTAGTCTTCACCCTTCAATGGAAGGACCTCGAGAAACACTTGGATTCGATCTGGGAAGCAAGCCAGACCCGATTCAAAGCGCTCCATGAACGAGAGAAGCAGCTGGATGCCAAAGAGTTAAAGATAGAGTCCAAAGAAAATGAGTTCCAGGAACGAGAGAAGCAGTTGGAGGCCAAAGAGTTAAAGGTGCAGTCCAAAGTGAATGAGTTGCTTGGAATCGAAAAGATGATTGAGGAGAAACAAAAAGACGCTATAGACAGCAAGAACTACTTGAATTCGCTCCAGTCATCAATCAAACAGCATACCGAATTGCTTGGAGAGAAGGAAAGACAAATGGAGAAACGAATTCAAGAATTGAATGCGGTTGAGAGGAATATAGAAGAGAAGTTGAACAAATCTCGTGAGGAACTGAATCTGATTGAGAGGACGATTATGGAACAGGGTGTAGAATTTGATTTGAAAGAAAAGCAAATTAAAGCGAAACAGAAGAAATTGAATTGGATTGAGTGGAGTATCGAAGAGAAGTTGAAATCCACAACTGAGGAGCAGAAGAAAGCATTTGATGTGAAACAAGAGCGTGTTAAAGAAGAGCAGAAACTGAGAGAGGAGAGACTGAAAGAGGAGGAGAAGTTGAAAGAGCATAACAAATCAGTAGTGGAAGGCTCTAAGACACTTAAGTCAAGAGAGGAAATAAAGAGGGAAATAGAACTGAAAGTGAAAGATCATTGCTCGCGTAAGATATCACTGGAGGAGTGGTCCTGTAATCTCGAACGTAAAGAGAAAGAATTAGAATTAAAAGAGAAGCAAGTTTTGTTAAAAGCTGAGGAACTTGATTCGCTTAATAAAAGAACCCTCAATGAGTTTCAAAAGCAATTTAGTTCACTCGAAAGTACAATACAAGAACGCGAAAAGCAATTGGAAGCAGACTCCCAGCGACTTCAAGTGAAAGAGAGGCAACTTGAAGTGAAAAGTAAAACACTGGAAAAGTTGCAGCAAGAACGTCAAAAGCAACTTGAAGTACAATGGAAAACTTGCACTACGAACGCGGAAAGCAACTCTAAGCGAAAGAGAGACTGTCTTCTTCATATAGTAGCTCAACATGAACAAGTAACTGAATTGTCCCGAGCCCTTGGTACGCAAATAAGGCACCTGCCAACAACATTAAAAGCTCCTAATGTCAAAACTAAGAATGTGAAAACTTCTACAGCAGCAATCTTTTCTTTTCCAAATCTTCAACTAGCTGCCACTGTCTCGTTTAATGCTATTATTGCTATTCATAATTTTATTAGACAGAAGAAACTATTGAAGGCTTATGGATGCATTTATAAGTTTAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

478

Amino Acids

56.7

Weight (kDa)

8.89

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1422
AarI CACCTGC 1 cut(s) 1254
AasI GACNNNNNNGTC 1 cut(s) 772
Acc36I ACCTGC 1 cut(s) 1254
AccB1I GGYRCC 1 cut(s) 1242
AccII CGCG 3 cut(s) 829, 1002, 1142
AciI CCGC 2 cut(s) 458, 1142
AcsI RAATTY 3 cut(s) 370, 441, 545
AfaI GTAC 3 cut(s) 988, 1116, 1231
AfeI AGCGCT 1 cut(s) 177
AfiI CCNNNNNNNGG 1 cut(s) 114
AflII CTTAAG 1 cut(s) 770
AflIII ACRYGT 1 cut(s) 60
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 6 cut(s) 72, 199, 917, 1190, 1265, 1337
AluI AGCT 6 cut(s) 72, 199, 917, 1190, 1265, 1337
Alw26I GTCTC 3 cut(s) 700, 1161, 1351
AlwNI CAGNNNCTG 1 cut(s) 694
Ama87I CYCGRG 2 cut(s) 125, 1218
Aor51HI AGCGCT 1 cut(s) 177
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 4 cut(s) 196, 1087, 1304, 1337
ApoI RAATTY 3 cut(s) 370, 441, 545
ArsI GACNNNNNNTTYG 1 cut(s) 36
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 2 cut(s) 121, 852
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 2 cut(s) 125, 1218
AvaII GGWCC 2 cut(s) 121, 852
BanI GGYRCC 1 cut(s) 1242
BanII GRGCYC 1 cut(s) 1225
BauI CACGAG 1 cut(s) 495
BbsI GAAGAC 2 cut(s) 94, 1166
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 4 cut(s) 208, 1099, 1316, 1324
BciT130I CCWGG 1 cut(s) 245
BcoDI GTCTC 3 cut(s) 700, 1161, 1351
BfaI CTAG 2 cut(s) 98, 1334
BfmI CTRYAG 2 cut(s) 348, 1299
BfoI RGCGCY 1 cut(s) 179
BfrI CTTAAG 1 cut(s) 770
BfuAI ACCTGC 1 cut(s) 1254
BisI GCNGC 4 cut(s) 197, 1088, 1305, 1338
BlsI GCNGC 4 cut(s) 198, 1089, 1306, 1339
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 2 cut(s) 121, 852
BmeT110I CYCGRG 2 cut(s) 125, 1218
BmgT120I GGNCC 2 cut(s) 121, 852
BmiI GGNNCC 1 cut(s) 1244
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 3 cut(s) 95, 193, 1403
BpiI GAAGAC 2 cut(s) 94, 1166
BpmI CTGGAG 2 cut(s) 362, 863
Bpu10I CCTNAGC 1 cut(s) 918
BsaJI CCNNGG 1 cut(s) 1225
BsaXI ACNNNNNCTCC 4 cut(s) 710, 740, 836, 866
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 3 cut(s) 379, 846, 1080
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 1225
BseGI GGATG 2 cut(s) 208, 1418
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 3 cut(s) 627, 686, 909
BseNI ACTGG 3 cut(s) 379, 846, 1080
BseRI GAGGAG 7 cut(s) 20, 344, 653, 716, 731, 734, 860
BseXI GCAGC 4 cut(s) 208, 1099, 1316, 1324
BseYI CCCAGC 1 cut(s) 1026
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 3 cut(s) 829, 1002, 1142
BshFI GGCC 1 cut(s) 269
BshNI GGYRCC 1 cut(s) 1242
BsiHKCI CYCGRG 2 cut(s) 125, 1218
BslFI GGGAC 1 cut(s) 1201
BslI CCNNNNNNNGG 1 cut(s) 114
BsmAI GTCTC 3 cut(s) 700, 1161, 1351
BsmFI GGGAC 1 cut(s) 1201
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 1 cut(s) 269
BsoBI CYCGRG 2 cut(s) 125, 1218
Bsp1286I GDGCHC 1 cut(s) 1225
Bsp143I GATC 2 cut(s) 144, 817
BspACI CCGC 2 cut(s) 458, 1142
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 3 cut(s) 628, 687, 910
BspFNI CGCG 3 cut(s) 829, 1002, 1142
BspLI GGNNCC 1 cut(s) 1244
BspMI ACCTGC 1 cut(s) 1254
BspQI GCTCTTC 1 cut(s) 678
BspT107I GGYRCC 1 cut(s) 1242
BspTI CTTAAG 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 820
BsrI ACTGG 3 cut(s) 379, 846, 1080
BssECI CCNNGG 1 cut(s) 1225
BssMI GATC 2 cut(s) 144, 817
BssSI CACGAG 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 1225
Bst2BI CACGAG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 3 cut(s) 50, 1172, 1345
Bst6I CTCTTC 3 cut(s) 471, 609, 678
BstAFI CTTAAG 1 cut(s) 770
BstC8I GCNNGC 3 cut(s) 74, 157, 827
BstDEI CTNAG 6 cut(s) 636, 695, 762, 918, 1155, 1281
BstF5I GGATG 2 cut(s) 208, 1418
BstFNI CGCG 3 cut(s) 829, 1002, 1142
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 2 cut(s) 147, 820
BstMAI GTCTC 3 cut(s) 700, 1161, 1351
BstMBI GATC 2 cut(s) 144, 817
BstMWI GCNNNNNNNGC 2 cut(s) 1240, 1364
BstNI CCWGG 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 2 cut(s) 348, 1299
BstUI CGCG 3 cut(s) 829, 1002, 1142
BstV1I GCAGC 4 cut(s) 208, 1099, 1316, 1324
BstV2I GAAGAC 2 cut(s) 94, 1166
BsuRI GGCC 1 cut(s) 269
BtsCI GGATG 2 cut(s) 208, 1418
BtsIMutI CAGTG 3 cut(s) 839, 1073, 1341
BveI ACCTGC 1 cut(s) 1254
Cac8I GCNNGC 3 cut(s) 74, 157, 827
CaiI CAGNNNCTG 1 cut(s) 694
CfoI GCGC 1 cut(s) 178
Cfr13I GGNCC 2 cut(s) 121, 852
CseI GACGC 1 cut(s) 353
Csp6I GTAC 3 cut(s) 987, 1115, 1230
CviAII CATG 3 cut(s) 94, 182, 1196
CviQI GTAC 3 cut(s) 987, 1115, 1230
DdeI CTNAG 6 cut(s) 636, 695, 762, 918, 1155, 1281
DpnI GATC 2 cut(s) 146, 819
DpnII GATC 2 cut(s) 144, 817
DrdI GACNNNNNNGTC 1 cut(s) 772
DseDI GACNNNNNNGTC 1 cut(s) 772
Eam1104I CTCTTC 3 cut(s) 471, 609, 678
EarI CTCTTC 3 cut(s) 471, 609, 678
Eco130I CCWWGG 1 cut(s) 1225
Eco24I GRGCYC 1 cut(s) 1225
Eco32I GATATC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 121, 852
Eco47III AGCGCT 1 cut(s) 177
Eco88I CYCGRG 2 cut(s) 125, 1218
EcoO109I RGGNCCY 1 cut(s) 121
EcoRI GAATTC 2 cut(s) 370, 441
EcoRII CCWGG 1 cut(s) 243
EcoRV GATATC 1 cut(s) 837
EcoT14I CCWWGG 1 cut(s) 1225
EcoT22I ATGCAT 1 cut(s) 1418
EcoT38I GRGCYC 1 cut(s) 1225
ErhI CCWWGG 1 cut(s) 1225
FaeI CATG 3 cut(s) 97, 185, 1199
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 1 cut(s) 1201
FatI CATG 3 cut(s) 93, 181, 1195
Fnu4HI GCNGC 4 cut(s) 197, 1088, 1305, 1338
FokI GGATG 2 cut(s) 215, 1425
FriOI GRGCYC 1 cut(s) 1225
Fsp4HI GCNGC 4 cut(s) 197, 1088, 1305, 1338
FspBI CTAG 2 cut(s) 98, 1334
GlaI GCGC 1 cut(s) 177
GluI GCNGC 4 cut(s) 197, 1088, 1305, 1338
GsaI CCCAGC 1 cut(s) 1030
GsuI CTGGAG 2 cut(s) 362, 863
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 1 cut(s) 269
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 178
Hin1II CATG 3 cut(s) 97, 185, 1199
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HinfI GANTC 7 cut(s) 140, 168, 224, 312, 508, 929, 1022
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 977
Hpy188I TCNGA 1 cut(s) 513
Hpy188III TCNNGA 6 cut(s) 127, 446, 497, 777, 863, 1218
Hpy8I GTNNAC 1 cut(s) 977
HpyAV CCTTC 6 cut(s) 19, 112, 119, 412, 749, 1396
HpyCH4III ACNGT 3 cut(s) 50, 1172, 1345
HpyCH4IV ACGT 3 cut(s) 60, 868, 1096
HpyCH4V TGCA 5 cut(s) 76, 286, 1087, 1131, 1416
HpyF10VI GCNNNNNNNGC 2 cut(s) 1240, 1364
HpyF3I CTNAG 6 cut(s) 636, 695, 762, 918, 1155, 1281
HpySE526I ACGT 3 cut(s) 60, 868, 1096
Hsp92II CATG 3 cut(s) 97, 185, 1199
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 2 cut(s) 144, 817
LguI GCTCTTC 1 cut(s) 678
LmnI GCTCC 4 cut(s) 183, 381, 640, 1270
Lsp1109I GCAGC 4 cut(s) 208, 1099, 1316, 1324
LweI GCATC 3 cut(s) 95, 193, 1403
MaeI CTAG 2 cut(s) 98, 1334
MaeII ACGT 3 cut(s) 60, 868, 1096
MaeIII GTNAC 1 cut(s) 1204
MalI GATC 2 cut(s) 146, 819
MboI GATC 2 cut(s) 144, 817
MfeI CAATTG 1 cut(s) 1010
MhlI GDGCHC 1 cut(s) 1225
MlyI GAGTC 2 cut(s) 233, 1016
MmeI TCCRAC 1 cut(s) 243
Mph1103I ATGCAT 1 cut(s) 1418
MspA1I CMGCKG 1 cut(s) 199
MspCI CTTAAG 1 cut(s) 770
MspR9I CCNGG 1 cut(s) 245
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 1 cut(s) 245
MvnI CGCG 3 cut(s) 829, 1002, 1142
MwoI GCNNNNNNNGC 2 cut(s) 1240, 1364
NdeII GATC 2 cut(s) 144, 817
NlaIII CATG 3 cut(s) 97, 185, 1199
NlaIV GGNNCC 1 cut(s) 1244
NsiI ATGCAT 1 cut(s) 1418
PaeR7I CTCGAG 1 cut(s) 125
PaqCI CACCTGC 1 cut(s) 1254
PciSI GCTCTTC 1 cut(s) 678
PctI GAATGC 1 cut(s) 460
PfeI GAWTC 5 cut(s) 140, 168, 312, 508, 929
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 4 cut(s) 198, 1089, 1306, 1339
PleI GAGTC 2 cut(s) 232, 1016
PpsI GAGTC 2 cut(s) 232, 1016
PpuMI RGGWCCY 1 cut(s) 121
PsiI TTATAA 1 cut(s) 1422
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 1026
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 1244
PspPI GGNCC 2 cut(s) 121, 852
PspPPI RGGWCCY 1 cut(s) 121
PstNI CAGNNNCTG 1 cut(s) 694
PvuII CAGCTG 1 cut(s) 199
RsaI GTAC 3 cut(s) 988, 1116, 1231
RsaNI GTAC 3 cut(s) 987, 1115, 1230
SapI GCTCTTC 1 cut(s) 678
SatI GCNGC 4 cut(s) 197, 1088, 1305, 1338
Sau3AI GATC 2 cut(s) 144, 817
Sau96I GGNCC 2 cut(s) 121, 852
SchI GAGTC 2 cut(s) 233, 1016
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 1225
SfaNI GCATC 3 cut(s) 95, 193, 1403
SfcI CTRYAG 2 cut(s) 348, 1299
Sfr274I CTCGAG 1 cut(s) 125
SinI GGWCC 2 cut(s) 121, 852
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 125, 770
SmoI CTYRAG 2 cut(s) 125, 770
SsiI CCGC 2 cut(s) 458, 1142
SspMI CTAG 2 cut(s) 98, 1334
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 1225
TaaI ACNGT 3 cut(s) 50, 1172, 1345
TaiI ACGT 3 cut(s) 63, 871, 1099
TaqI TCGA 6 cut(s) 126, 143, 315, 612, 864, 981
TatI WGTACW 2 cut(s) 986, 1114
TfiI GAWTC 5 cut(s) 140, 168, 312, 508, 929
TscAI CASTG 3 cut(s) 846, 1080, 1348
TseI GCWGC 4 cut(s) 196, 1087, 1304, 1337
TspDTI ATGAA 4 cut(s) 198, 1169, 1212, 1361
TspRI CASTG 3 cut(s) 846, 1080, 1348
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 2 cut(s) 121, 852
XapI RAATTY 3 cut(s) 370, 441, 545
XhoI CTCGAG 1 cut(s) 125
XspI CTAG 2 cut(s) 98, 1334
Zsp2I ATGCAT 1 cut(s) 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.