Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0008143

Physical Location & Seq

Genomic Coordinates Forward (+)

50115266 .. 50117941

Gene Structure 2676 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00429960.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGACATTGGATTCGAAGGAATACGAGTTGCAGAAGAACAGTATGAGAAAGATGTACGAGAGCTTGCACTCCCAAGCATCTTCCATGCTAGTCTTCACCCTTCAGTGGAAGGAACTGGAGGGATGCTTAGATTCGATCCGGGATGCGACCCAGACCCGACTCGAAGAGCTCCATGAACGAGAGCAGCAGTTGGATGCCAAAGAGTTAAAGATGGAGTCCAAAGCAAATGAGTTCCAGGAACGAGAGAAGCAGTTGGAGGCCAAAGAGTTAAAGATCCAGTCCGAAGTCAATGAGGCGTTTGGACTCGAAAAGCTGATTAAGGAGAAACAAAAAGAGGCTCTAGACAGCAAGAACTACTTGAATTCGCTCCAGTTATCGATCAAAGAGCATACCGAATCAGAGAAAGAGCTTGGAGCCAAGGAAAGACAAATAGAGAGACAAATTCAAGAATTGAATGGGGTTGAGAGGAATATAGAAGAGGAGTTGAAGAAATCTCATGAGGAACTGAATCAGATTCAGAGGGCGATTAAGGAGCAAGGTGTAGAATTTGATTTGAAAGAAAAGCAAATTAAAGTGAAAGAGGAGAAATTGAATCAGATTGAGAAGAATATCGAAGAGAAGTTGAAATTGGCAATTGAGGAGGAGAAGAAAGCATTTGATCTGAAACAAGAGCGTGTTAAAGAAGAGCAGAAACTGAGAGAGGAGAGACTGCAAGAGGAGGAGAAGTTGAAAGAGCACAAGAAATCAGTAGTGGAAGCCTCTAAGACACTTAAGTCAAGAGAGGAAATAAAGAGGGAACTAGAACTGAAAGTGAAAGATCATTGCTTGAGAAAGAAATCAATGGAGGAGTGGTCCTGTAATCTCAAACGTAAAGAGAGAGAATTAGAATTGAAAGAGAAGCAAGTTTTGTCAAAAGCTGAGGAACTTGACTCGCTTAATAAAAGGACCCTCAATGAGTTTCAGTTGATGGAAAAGGAACTTAGTTCAGTCGAAAGTACGTTACAAGAACGTGAAAAGAAATTGGAAGCAGCCTCCCAGCGACTTCAAGTGAAACAGAGGCAACTTGAAGTGAAAAGTGAAACAATGGAAAAGTTTCAGCAAGAATGCAAAAAGCAACTTGAAGCGAAAGAGAGACAACTTGAAGTAAAAAGTGATACAATGGAAAAATTGCATCAGGAACGCGAAAAGAAACTTGAAGCCAAAGGGAGACAACTTGAAGAGCAGAGCAAAGAGCTTGAATTGACAAAGAAACAATTTGGCTGTCAAATGAAAGTCAAAAGTGAGCCACTGGAAAATACCCCAGCTGCTAACAATGCCATTGTTTCTTCATCTGCAAGTGATCATTCCAAAATCAATATCACGGATGGTAGAGGCTTGCAACTATTCATGTATGAGCATATGAAGAGAAATGATTCAGTGAGCAAAGAAATCTCAGCTGTTCTTCAACAAGAGTCATTAGAGCCAGCAAAATTGGTTTTGGATGCAATGGAAGGGTTTCACCCTTCAAATTCGACTGTGGACAATAGGGAGTTTGATTTGGTAATTATTAGAAGGACATGTATTCTTTTGTTGGAAGAGTTAAAGAGAGTCTCGGCGCAAATTAATCCTCAGGTGAGCGAACAAGCAATGAAGTTGGCCGGTGATTGGAAGGCTAAGATGACGGTGGGGACTGAGAATTGGTTAGAGGTTTTGGGATTTTTGCGGCTTGTTACTACATATGAATTGACCTCTGCCTATGATGCAAATGAGCTTCAAGGTCTTCTTGATATAGTAGCTCAACATGAACAAGCAACTGAATTATCCCGAGCCCTTGGTACCACAGATAAGGCAGCTGCCAACAACATTAGTGTTGCATCTGTTAAAAATGAGAAAGCAGAATCGCCTGCACGAGCAACTTTTTCTTCTCCAAATCTTCAACCAACTGCCAAGAACAACATTATTGTCGCTCCTGTCACAACTAAGAAAGCTCCTGATGTCGAAACTAAGAATGTGAAAAGTTCTGCAGCTGCAATCTTTTCTTCTCCTAATCTTCAACCAGCTACCACTGTCCCAGCTAACGCTATTATTGCTATTCAGAATTTTATTAGAGAGAAGAAACTGGTGAAGGCTTATGGACGCATTCACAAATTCAAGTTATTAGACAAGTTCCCTCCAGCTCAAGTCTTAAAAGAATATGTTGAGAAGGCCATGAAGTGCATGGAAAATAGCGAGAGAAAAGACATGCTCCATCAACAGGATCAGGCTGTAGACGAAGGAATATCTGATCTACGACGTGCTATTGAATGCATCAAATATCACAACCTTGAGTCGGAATACCCTTCTGAGAGCATTGAGAAACAAATAGTTGTGCTACAGAAATTTATGGAGGATCGGAAAAACCAAAATCAGAAAAAACGCTTATGCAGCACAATTGATTCCAGCTTTCAACAGCAGGAACATAAATTTCAGCGGACATCCTTATCAGCGGGAAGACCATATGGATTGCACCCTGTCTATCCGAATCCAGGTTCATCATTTTACAATGGACAGTTTAGTATTCCTTCCAACAATCCCGTGCAGTTTAGTATGCCTGCCAACTGCGTTAATTATGAGTTCCCTAGAATATATCCATACCCTCCATACCCTCGCCCTTTTGTATATAGACACCCTGGGTATGGCCTTCAAAAGCCATATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

891

Amino Acids

103.22

Weight (kDa)

6.85

Isoelectric Point (pI)

55.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	455 - 609	2.9e-34	Frigida-like protein
Frigida	PF07899	691 - 794	1.6e-18	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008143)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04251
rosa_chinensis	RchiOBHm_Chr3g0452961
rosa_laevigata	RLG00000025558 RLG00000035001 RLG00000035002
rosa_multiflora	Rmu_sc0008989.1_g000006
rosa_roxburghii	Rroxscaffold_1G00024630 Rroxscaffold_6G00429960 Rroxscaffold_6G00430020
rosa_rugosa	Rorug02G0643800 Rorug02G0643800 Rorug02G0643800 Rorug02G0643800 Rorug05G0294400
rosa_samantha	Rh3AG046900 Rh3BG049300 Rh3CG048000 Rh3DG048900 Rh5AG364000 Rh5BG375900 Rh5CG398400 Rh5DG389100
rosa_wichuraiana	Rw3G003680 Rw5G034230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	772
Acc65I	GGTACC	1 cut(s)	1814
AccB1I	GGYRCC	1 cut(s)	1814
AccI	GTMKAC	1 cut(s)	2247
AccII	CGCG	1 cut(s)	1182
AciI	CCGC	3 cut(s)	1702, 2449, 2465
AclWI	GGATC	4 cut(s)	130, 268, 2244, 2376
AcoI	YGGCCR	1 cut(s)	1635
AcsI	RAATTY	8 cut(s)	361, 441, 545, 1507, 2077, 2126, 2357, 2441
AcuI	CTGAAG	1 cut(s)	86
AfaI	GTAC	3 cut(s)	56, 997, 1816
AfiI	CCNNNNNNNGG	6 cut(s)	105, 1644, 2233, 2308, 2504, 2654
AflII	CTTAAG	1 cut(s)	770
AflIII	ACRYGT	1 cut(s)	1556
AjiI	CACGTC	1 cut(s)	2273
AjnI	CCWGG	3 cut(s)	234, 2503, 2647
AjuI	GAANNNNNNNTTGG	2 cut(s)	611, 643
Alw21I	GWGCWC	2 cut(s)	171, 738
Alw26I	GTCTC	5 cut(s)	430, 700, 1126, 1201, 1594
AlwI	GGATC	4 cut(s)	130, 268, 2244, 2376
AlwNI	CAGNNNCTG	1 cut(s)	694
Ama87I	CYCGRG	1 cut(s)	1803
AoxI	GGCC	4 cut(s)	258, 1635, 2184, 2656
ApeKI	GCWGC	8 cut(s)	184, 1028, 1304, 1829, 1832, 2003, 2006, 2403
ApoI	RAATTY	8 cut(s)	361, 441, 545, 1507, 2077, 2126, 2357, 2441
ArsI	GACNNNNNNTTYG	1 cut(s)	27
AseI	ATTAAT	1 cut(s)	1602
Asp700I	GAANNNNTTC	3 cut(s)	1092, 1411, 1494
Asp718I	GGTACC	1 cut(s)	1814
AspLEI	GCGC	1 cut(s)	1597
AspS9I	GGNCC	2 cut(s)	852, 945
AsuC2I	CCSGG	1 cut(s)	140
AsuHPI	GGTGA	5 cut(s)	88, 1490, 1624, 1652, 2113
AsuII	TTCGAA	1 cut(s)	14
AvaI	CYCGRG	1 cut(s)	1803
AvaII	GGWCC	2 cut(s)	852, 945
AxyI	CCTNAGG	1 cut(s)	1608
BanI	GGYRCC	1 cut(s)	1814
BanII	GRGCYC	2 cut(s)	171, 1810
BauI	CACGAG	1 cut(s)	1887
BbsI	GAAGAC	3 cut(s)	85, 1751, 2476
Bbv12I	GWGCWC	2 cut(s)	171, 738
BbvCI	CCTCAGC	1 cut(s)	918
BbvI	GCAGC	8 cut(s)	196, 1040, 1291, 1819, 1841, 1993, 2015, 2415
BccI	CCATC	4 cut(s)	205, 961, 1358, 2235
BciT130I	CCWGG	3 cut(s)	236, 2505, 2649
BclI	TGATCA	1 cut(s)	1339
BcnI	CCSGG	1 cut(s)	140
BcoDI	GTCTC	5 cut(s)	430, 700, 1126, 1201, 1594
BfaI	CTAG	4 cut(s)	89, 341, 800, 2598
BfmI	CTRYAG	3 cut(s)	2001, 2244, 2351
BfrI	CTTAAG	1 cut(s)	770
BisI	GCNGC	9 cut(s)	185, 1029, 1305, 1703, 1830, 1833, 2004, 2007, 2404
BlsI	GCNGC	9 cut(s)	186, 1030, 1306, 1704, 1831, 1834, 2005, 2008, 2405
Bme1390I	CCNGG	4 cut(s)	140, 236, 2505, 2649
Bme18I	GGWCC	2 cut(s)	852, 945
BmeT110I	CYCGRG	1 cut(s)	1803
BmgBI	CACGTC	1 cut(s)	2273
BmgT120I	GGNCC	2 cut(s)	852, 945
BmiI	GGNNCC	3 cut(s)	415, 947, 1816
BmrFI	CCNGG	4 cut(s)	140, 236, 2505, 2649
BmsI	GCATC	9 cut(s)	86, 113, 133, 184, 1180, 1471, 1729, 1862, 2295
BpiI	GAAGAC	3 cut(s)	85, 1751, 2476
BpmI	CTGGAG	3 cut(s)	137, 353, 2136
Bpu10I	CCTNAGC	1 cut(s)	918
Bpu14I	TTCGAA	1 cut(s)	14
BpuEI	CTTGAG	3 cut(s)	847, 2142, 2324
BpuMI	CCSGG	1 cut(s)	140
Bsa29I	ATCGAT	1 cut(s)	377
BsaJI	CCNNGG	4 cut(s)	417, 1810, 2647, 2648
BsaXI	ACNNNNNCTCC	4 cut(s)	710, 740, 836, 866
Bsc4I	CCNNNNNNNGG	6 cut(s)	105, 1644, 2233, 2308, 2504, 2654
Bse118I	RCCGGY	1 cut(s)	1637
Bse1I	ACTGG	5 cut(s)	120, 277, 370, 1293, 2103
Bse21I	CCTNAGG	1 cut(s)	1608
Bse3DI	GCAATG	3 cut(s)	820, 1491, 1632
BseBI	CCWGG	3 cut(s)	236, 2505, 2649
BseCI	ATCGAT	1 cut(s)	377
BseDI	CCNNGG	4 cut(s)	417, 1810, 2647, 2648
BseGI	GGATG	6 cut(s)	128, 148, 199, 1369, 1486, 2453
BseLI	CCNNNNNNNGG	6 cut(s)	105, 1644, 2233, 2308, 2504, 2654
BseMI	GCAATG	3 cut(s)	820, 1491, 1632
BseMII	CTCAG	6 cut(s)	686, 909, 1446, 1622, 1662, 2313
BseNI	ACTGG	5 cut(s)	120, 277, 370, 1293, 2103
BseRI	GAGGAG	8 cut(s)	494, 596, 653, 656, 716, 731, 734, 860
BseXI	GCAGC	8 cut(s)	196, 1040, 1291, 1819, 1841, 1993, 2015, 2415
BseYI	CCCAGC	3 cut(s)	1035, 1300, 2050
BsgI	GTGCAG	2 cut(s)	1869, 2576
Bsh1236I	CGCG	1 cut(s)	1182
BshFI	GGCC	4 cut(s)	260, 1637, 2186, 2658
BshNI	GGYRCC	1 cut(s)	1814
BshVI	ATCGAT	1 cut(s)	377
BsiHKAI	GWGCWC	2 cut(s)	171, 738
BsiHKCI	CYCGRG	1 cut(s)	1803
BsiSI	CCGG	2 cut(s)	139, 1638
BslFI	GGGAC	2 cut(s)	1681, 2033
BslI	CCNNNNNNNGG	6 cut(s)	105, 1644, 2233, 2308, 2504, 2654
BsmAI	GTCTC	5 cut(s)	430, 700, 1126, 1201, 1594
BsmFI	GGGAC	2 cut(s)	1681, 2033
BsmI	GAATGC	3 cut(s)	1109, 2118, 2288
BsnI	GGCC	4 cut(s)	260, 1637, 2186, 2658
BsoBI	CYCGRG	1 cut(s)	1803
Bsp119I	TTCGAA	1 cut(s)	14
Bsp1286I	GDGCHC	3 cut(s)	171, 738, 1810
Bsp143I	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
BspACI	CCGC	3 cut(s)	1702, 2449, 2465
BspANI	GGCC	4 cut(s)	260, 1637, 2186, 2658
BspCNI	CTCAG	6 cut(s)	687, 910, 1445, 1621, 1663, 2314
BspDI	ATCGAT	1 cut(s)	377
BspFNI	CGCG	1 cut(s)	1182
BspHI	TCATGA	1 cut(s)	496
BspLI	GGNNCC	3 cut(s)	415, 947, 1816
BspMAI	CTGCAG	1 cut(s)	2005
BspPI	GGATC	4 cut(s)	130, 268, 2244, 2376
BspQI	GCTCTTC	3 cut(s)	159, 678, 1212
BspT104I	TTCGAA	1 cut(s)	14
BspT107I	GGYRCC	1 cut(s)	1814
BspTI	CTTAAG	1 cut(s)	770
BsrDI	GCAATG	3 cut(s)	820, 1491, 1632
BsrFI	RCCGGY	1 cut(s)	1637
BsrI	ACTGG	5 cut(s)	120, 277, 370, 1293, 2103
BssAI	RCCGGY	1 cut(s)	1637
BssECI	CCNNGG	4 cut(s)	417, 1810, 2647, 2648
BssMI	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
BssSI	CACGAG	1 cut(s)	1887
BssT1I	CCWWGG	2 cut(s)	417, 1810
Bst2BI	CACGAG	1 cut(s)	1887
Bst2UI	CCWGG	3 cut(s)	236, 2505, 2649
Bst4CI	ACNGT	5 cut(s)	41, 1516, 1663, 2047, 2529
Bst6I	CTCTTC	7 cut(s)	159, 471, 609, 678, 1212, 1397, 1569
BstAFI	CTTAAG	1 cut(s)	770
BstBI	TTCGAA	1 cut(s)	14
BstC8I	GCNNGC	5 cut(s)	65, 1376, 1464, 1884, 2571
BstF5I	GGATG	6 cut(s)	128, 148, 199, 1369, 1486, 2453
BstFNI	CGCG	1 cut(s)	1182
BstHHI	GCGC	1 cut(s)	1597
BstKTI	GATC	9 cut(s)	138, 276, 381, 661, 820, 1342, 2239, 2266, 2371
BstMAI	GTCTC	5 cut(s)	430, 700, 1126, 1201, 1594
BstMBI	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
BstMWI	GCNNNNNNNGC	4 cut(s)	1313, 1739, 2066, 2403
BstNI	CCWGG	3 cut(s)	236, 2505, 2649
BstNSI	RCATGY	2 cut(s)	1560, 2224
BstSCI	CCNGG	4 cut(s)	138, 234, 2503, 2647
BstSFI	CTRYAG	3 cut(s)	2001, 2244, 2351
BstUI	CGCG	1 cut(s)	1182
BstV1I	GCAGC	8 cut(s)	196, 1040, 1291, 1819, 1841, 1993, 2015, 2415
BstV2I	GAAGAC	3 cut(s)	85, 1751, 2476
BstX2I	RGATCY	1 cut(s)	273
BstYI	RGATCY	1 cut(s)	273
Bsu15I	ATCGAT	1 cut(s)	377
Bsu36I	CCTNAGG	1 cut(s)	1608
BsuRI	GGCC	4 cut(s)	260, 1637, 2186, 2658
BsuTUI	ATCGAT	1 cut(s)	377
BtrI	CACGTC	1 cut(s)	2273
BtsCI	GGATG	6 cut(s)	128, 148, 199, 1369, 1486, 2453
BtsIMutI	CAGTG	4 cut(s)	110, 1286, 1422, 2043
Cac8I	GCNNGC	5 cut(s)	65, 1376, 1464, 1884, 2571
CaiI	CAGNNNCTG	1 cut(s)	694
CciI	TCATGA	1 cut(s)	496
CfoI	GCGC	1 cut(s)	1597
Cfr10I	RCCGGY	1 cut(s)	1637
Cfr13I	GGNCC	2 cut(s)	852, 945
ClaI	ATCGAT	1 cut(s)	377
CseI	GACGC	1 cut(s)	2124
Csp6I	GTAC	3 cut(s)	55, 996, 1815
CviAII	CATG	9 cut(s)	85, 173, 497, 1387, 1557, 1781, 2188, 2197, 2221
CviQI	GTAC	3 cut(s)	55, 996, 1815
DpnI	GATC	9 cut(s)	137, 275, 380, 660, 819, 1341, 2238, 2265, 2370
DpnII	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
DrdI	GACNNNNNNGTC	1 cut(s)	772
DseDI	GACNNNNNNGTC	1 cut(s)	772
EaeI	YGGCCR	1 cut(s)	1635
Eam1104I	CTCTTC	7 cut(s)	159, 471, 609, 678, 1212, 1397, 1569
EarI	CTCTTC	7 cut(s)	159, 471, 609, 678, 1212, 1397, 1569
Ecl136II	GAGCTC	1 cut(s)	169
Eco130I	CCWWGG	2 cut(s)	417, 1810
Eco24I	GRGCYC	2 cut(s)	171, 1810
Eco47I	GGWCC	2 cut(s)	852, 945
Eco53kI	GAGCTC	1 cut(s)	169
Eco57I	CTGAAG	1 cut(s)	86
Eco81I	CCTNAGG	1 cut(s)	1608
Eco88I	CYCGRG	1 cut(s)	1803
EcoICRI	GAGCTC	1 cut(s)	169
EcoO109I	RGGNCCY	1 cut(s)	945
EcoRI	GAATTC	1 cut(s)	361
EcoRII	CCWGG	3 cut(s)	234, 2503, 2647
EcoT14I	CCWWGG	2 cut(s)	417, 1810
EcoT22I	ATGCAT	1 cut(s)	2288
EcoT38I	GRGCYC	2 cut(s)	171, 1810
ErhI	CCWWGG	2 cut(s)	417, 1810
FaeI	CATG	9 cut(s)	88, 176, 500, 1390, 1560, 1784, 2191, 2200, 2224
FalI	AAGNNNNNCTT	4 cut(s)	341, 373, 1746, 1778
FaqI	GGGAC	2 cut(s)	1681, 2033
FatI	CATG	9 cut(s)	84, 172, 496, 1386, 1556, 1780, 2187, 2196, 2220
FauI	CCCGC	1 cut(s)	2458
FauNDI	CATATG	3 cut(s)	1398, 1717, 2476
FbaI	TGATCA	1 cut(s)	1339
FblI	GTMKAC	1 cut(s)	2247
Fnu4HI	GCNGC	9 cut(s)	185, 1029, 1305, 1703, 1830, 1833, 2004, 2007, 2404
FokI	GGATG	6 cut(s)	135, 155, 206, 1376, 1493, 2440
FriOI	GRGCYC	2 cut(s)	171, 1810
Fsp4HI	GCNGC	9 cut(s)	185, 1029, 1305, 1703, 1830, 1833, 2004, 2007, 2404
FspBI	CTAG	4 cut(s)	89, 341, 800, 2598
GlaI	GCGC	1 cut(s)	1596
GluI	GCNGC	9 cut(s)	185, 1029, 1305, 1703, 1830, 1833, 2004, 2007, 2404
GsaI	CCCAGC	3 cut(s)	1039, 1304, 2054
GsuI	CTGGAG	3 cut(s)	137, 353, 2136
HaeIII	GGCC	4 cut(s)	260, 1637, 2186, 2658
HapII	CCGG	2 cut(s)	139, 1638
HgaI	GACGC	1 cut(s)	2124
HhaI	GCGC	1 cut(s)	1597
Hin1II	CATG	9 cut(s)	88, 176, 500, 1390, 1560, 1784, 2191, 2200, 2224
Hin6I	GCGC	1 cut(s)	1595
HinP1I	GCGC	1 cut(s)	1595
HpaII	CCGG	2 cut(s)	139, 1638
HphI	GGTGA	5 cut(s)	88, 1490, 1624, 1652, 2113
Hpy166II	GTNNAC	2 cut(s)	1519, 2248
Hpy188III	TCNNGA	8 cut(s)	341, 446, 497, 777, 1175, 1763, 1803, 1970
Hpy8I	GTNNAC	2 cut(s)	1519, 2248
Hpy99I	CGWCG	1 cut(s)	2274
HpyCH4III	ACNGT	5 cut(s)	41, 1516, 1663, 2047, 2529
HpyCH4IV	ACGT	4 cut(s)	868, 998, 1009, 2272
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1313, 1739, 2066, 2403
HpySE526I	ACGT	4 cut(s)	868, 998, 1009, 2272
Hsp92II	CATG	9 cut(s)	88, 176, 500, 1390, 1560, 1784, 2191, 2200, 2224
HspAI	GCGC	1 cut(s)	1595
KpnI	GGTACC	1 cut(s)	1818
Ksp22I	TGATCA	1 cut(s)	1339
Kzo9I	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
LguI	GCTCTTC	3 cut(s)	159, 678, 1212
LmnI	GCTCC	7 cut(s)	174, 372, 413, 532, 1951, 1972, 2229
Lsp1109I	GCAGC	8 cut(s)	196, 1040, 1291, 1819, 1841, 1993, 2015, 2415
LweI	GCATC	9 cut(s)	86, 113, 133, 184, 1180, 1471, 1729, 1862, 2295
MaeI	CTAG	4 cut(s)	89, 341, 800, 2598
MaeII	ACGT	4 cut(s)	868, 998, 1009, 2272
MaeIII	GTNAC	3 cut(s)	999, 1708, 1951
MalI	GATC	9 cut(s)	137, 275, 380, 660, 819, 1341, 2238, 2265, 2370
MboI	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
MfeI	CAATTG	2 cut(s)	633, 2409
MflI	RGATCY	1 cut(s)	273
MhlI	GDGCHC	3 cut(s)	171, 738, 1810
MlyI	GAGTC	7 cut(s)	153, 224, 297, 923, 1460, 1596, 2315
MmeI	TCCRAC	5 cut(s)	171, 234, 1551, 2289, 2568
Mph1103I	ATGCAT	1 cut(s)	2288
MroXI	GAANNNNTTC	3 cut(s)	1092, 1411, 1494
MspA1I	CMGCKG	6 cut(s)	1304, 1436, 1832, 2006, 2449, 2465
MspCI	CTTAAG	1 cut(s)	770
MspI	CCGG	2 cut(s)	139, 1638
MspR9I	CCNGG	4 cut(s)	140, 236, 2505, 2649
MunI	CAATTG	2 cut(s)	633, 2409
Mva1269I	GAATGC	3 cut(s)	1109, 2118, 2288
MvaI	CCWGG	3 cut(s)	236, 2505, 2649
MvnI	CGCG	1 cut(s)	1182
MwoI	GCNNNNNNNGC	4 cut(s)	1313, 1739, 2066, 2403
NciI	CCSGG	1 cut(s)	140
NdeI	CATATG	3 cut(s)	1398, 1717, 2476
NdeII	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
NlaIII	CATG	9 cut(s)	88, 176, 500, 1390, 1560, 1784, 2191, 2200, 2224
NlaIV	GGNNCC	3 cut(s)	415, 947, 1816
NmeAIII	GCCGAG	1 cut(s)	1571
NmuCI	GTSAC	1 cut(s)	1951
NsiI	ATGCAT	1 cut(s)	2288
NspI	RCATGY	2 cut(s)	1560, 2224
NspV	TTCGAA	1 cut(s)	14
PagI	TCATGA	1 cut(s)	496
PasI	CCCWGGG	1 cut(s)	2648
PciI	ACATGT	1 cut(s)	1556
PciSI	GCTCTTC	3 cut(s)	159, 678, 1212
PctI	GAATGC	3 cut(s)	1109, 2118, 2288
PdmI	GAANNNNTTC	3 cut(s)	1092, 1411, 1494
PfoI	TCCNGGA	2 cut(s)	138, 234
PkrI	GCNGC	9 cut(s)	186, 1030, 1306, 1704, 1831, 1834, 2005, 2008, 2405
PleI	GAGTC	7 cut(s)	153, 223, 297, 923, 1459, 1595, 2314
PpsI	GAGTC	7 cut(s)	153, 223, 297, 923, 1459, 1595, 2314
PpuMI	RGGWCCY	1 cut(s)	945
PscI	ACATGT	1 cut(s)	1556
PshBI	ATTAAT	1 cut(s)	1602
Psp124BI	GAGCTC	1 cut(s)	171
Psp5II	RGGWCCY	1 cut(s)	945
Psp6I	CCWGG	3 cut(s)	234, 2503, 2647
PspFI	CCCAGC	3 cut(s)	1035, 1300, 2050
PspGI	CCWGG	3 cut(s)	234, 2503, 2647
PspN4I	GGNNCC	3 cut(s)	415, 947, 1816
PspPI	GGNCC	2 cut(s)	852, 945
PspPPI	RGGWCCY	1 cut(s)	945
PstI	CTGCAG	1 cut(s)	2005
PstNI	CAGNNNCTG	1 cut(s)	694
PsuI	RGATCY	1 cut(s)	273
PvuII	CAGCTG	4 cut(s)	1304, 1436, 1832, 2006
RsaI	GTAC	3 cut(s)	56, 997, 1816
RsaNI	GTAC	3 cut(s)	55, 996, 1815
SacI	GAGCTC	1 cut(s)	171
SapI	GCTCTTC	3 cut(s)	159, 678, 1212
SatI	GCNGC	9 cut(s)	185, 1029, 1305, 1703, 1830, 1833, 2004, 2007, 2404
Sau3AI	GATC	9 cut(s)	135, 273, 378, 658, 817, 1339, 2236, 2263, 2368
Sau96I	GGNCC	2 cut(s)	852, 945
SchI	GAGTC	7 cut(s)	153, 224, 297, 923, 1460, 1596, 2315
ScrFI	CCNGG	4 cut(s)	140, 236, 2505, 2649
SduI	GDGCHC	3 cut(s)	171, 738, 1810
SfaNI	GCATC	9 cut(s)	86, 113, 133, 184, 1180, 1471, 1729, 1862, 2295
SfcI	CTRYAG	3 cut(s)	2001, 2244, 2351
SfuI	TTCGAA	1 cut(s)	14
SinI	GGWCC	2 cut(s)	852, 945
SmlI	CTYRAG	4 cut(s)	770, 826, 2157, 2303
SmoI	CTYRAG	4 cut(s)	770, 826, 2157, 2303
SsiI	CCGC	3 cut(s)	1702, 2449, 2465
SspMI	CTAG	4 cut(s)	89, 341, 800, 2598
SstI	GAGCTC	1 cut(s)	171
StyD4I	CCNGG	4 cut(s)	138, 234, 2503, 2647
StyI	CCWWGG	2 cut(s)	417, 1810
TaaI	ACNGT	5 cut(s)	41, 1516, 1663, 2047, 2529
TaiI	ACGT	4 cut(s)	871, 1001, 1012, 2275
TaqI	TCGA	9 cut(s)	14, 134, 162, 306, 377, 612, 990, 1511, 1977
TauI	GCSGC	1 cut(s)	1705
TscAI	CASTG	4 cut(s)	110, 1293, 1422, 2050
TseFI	GTSAC	1 cut(s)	1951
TseI	GCWGC	8 cut(s)	184, 1028, 1304, 1829, 1832, 2003, 2006, 2403
Tsp45I	GTSAC	1 cut(s)	1951
TspGWI	ACGGA	1 cut(s)	1376
TspRI	CASTG	4 cut(s)	110, 1293, 1422, 2050
Vha464I	CTTAAG	1 cut(s)	770
VpaK11BI	GGWCC	2 cut(s)	852, 945
VspI	ATTAAT	1 cut(s)	1602
XapI	RAATTY	8 cut(s)	361, 441, 545, 1507, 2077, 2126, 2357, 2441
XbaI	TCTAGA	1 cut(s)	340
XceI	RCATGY	2 cut(s)	1560, 2224
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2194
XmiI	GTMKAC	1 cut(s)	2247
XmnI	GAANNNNTTC	3 cut(s)	1092, 1411, 1494
XspI	CTAG	4 cut(s)	89, 341, 800, 2598
Zsp2I	ATGCAT	1 cut(s)	2288

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.