Rw3G003680

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008143
Physical Location & Seq
Forward (+)
3131699 .. 3134377
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003680.1

Sequence Viewer

Length: 2679 bp
ATGGAGAAGATGACATTGGATTCGAAGGAATATGAGTTGAAGAAGAACAGTATGAGAAAGATGTACGAGAGCTTGCACTCCCAAGCATCTTCCATGCTAGTCTTCACCCTTCAGTGGAAGGAACTGGAGGGATGCTTAGATTCGATCCGGGATGCGACCCAGACCCGACTCGAAGAGCTCCATGAACGAGAGCAGCAGTTGGATGCCAAAGAGTTAAAGATGGAGTCCAAAGCAAATGAGTTCCAGGAACGAGAGAAGCAGTTGGAGGCCAAAGAGTTAAAGATCCAGTCCGAAGTCAATGAGGCGTTTGGACTCGAAAAGCTGATTAAGGAGAAACAAAAAGAGGCTCTAGACAGCAAGAACTACTTGAATTCGCTCCAGTTATTGATCAAAGAGCATACCGAATCAGAGAAAGAGCTTGGAGCCAAGGAAAGACAAATAGAGAGACGAATTCAAGAATTGAATGGGGTTGAGAGGAATATAGAAGAGAAGTTGAAGAAATCTCGTGAGGAACTGAATCAGATTCAGATGGGGATTAAGGAGCAGGGTGTAGAATTTGATTTGAAAGAAAAGCAAATTAAAGTGAAAGAGGAGAAATTGAATCGGATTGAGAAGAATATCGAAGAGAAGTTGAAATTGGCAATTGAGGAGGAGAAGAAAGCATTTGATCCGAAACAAGAACGTGTTACAGAAGAGCAGAAACTGAGAGAGGAGAGACTGCAAGAGGAGGAGAAGTTGAAAGAGCACAAGAAATCAGTAGTGGATGCCTCTAAGACACTCAAGTCAAGAGAGGAAATAAAGAGGGAACTAGAACTGAAAGTGAAAGATCATTGCTCGAGAAAGAAATCAATGGAGGAGTGGTCCTGTAATCTCGAACGTAAAGAGAGAGAATTAGAATTGAAAGAGAAGCAAGTTTTGTCAAAAGCAGAGGAACTTGACTCGCTTAATAAAAGGACCCTCAATGAGTTTCAGTTGATGGAAAAGGAACTTAGTTCAGTCGAAAGTACGATACAAGAACGTGAAAAGAAATTGGAAGCAGCCTCCCACCGACTTCAAGTGAAAGAGAGGCAACTTGAACTGAAAAGTGAAACAATGGAAAAGTTGCAGCAAGAATGCAAAAAGCAACTTGAAGCGAGAGAGAGACAACTTGAAGTCAAAAGTGGTACAATGGAAAAATTGCATCAAGAACGCGAAAAGAAACTTAAAGTGAAAGAGAGACTACTTGAAGAGCAGAGCAAAGAGCTTGAATTGACAAAGAAACAATTTGGTTGTCAAATGAAAGTAAAAAGTGAGCCACTGGAAAATACCCCAGCTGCTAACAATGCCATTGTTTCTTCATCTGCAAGTGATCATTCCAAAATCAATATCAGGGATGGTAGAGGCTTGCAACTATTCATGTATGAGCATATGAAGAGAAATGATTCGGTGAGCAAAGAAATCTCAGCTGTTCTTCAACAAGAGTCATTAGAGCCAGCAAAATTGGTTTTGGATGCAATGGAAGGGTTTCACCCTTCAAATTCGACTGTGGACAATAGGGAGTTTGATTTGGTAATTATTAGAAGGACATGTATTCTTTTGTTGGAAGAGTTAAAGAGAGTCTCGGCGCAAATTAATCCTCAGGTGAGAGAACAAGCAATAAAGTTGGCCGGTGATTGGAAGGCTAAGATGACGGTGGGGACTGAGAATTGGTTAGAGGTTTTGGGATTTTTGCGGCTTGTTACTACATATGAATTGACCTCTGCCTATGATGCAAATGAGCTTCAAGGTCTTCTTGATATAGTAGCTCAACATGAACAAGCAACTGAATTATCCCGAGCCCTTGGTACCACAGATAAGGCAGCTGCCAACAACATTAGTGTTGCATCTGTTAAAAATGAGAAAGCAGAATCGCCTGCACGAGAAACTTTTTCTTCTCGAAATCTTCAACCAACTGCCAAGAACAACATTATTGTCGCTCCTGTCACAACTAAGAAAGCTCCTGATGTCGAAACTAAGAATGCGAAAAGTTCTGCAGCAGCAATCTTTTCTTCTCCTAATCTTCAACCAGCTACCACTGTCCCAGCTAATGCTATTATTGCTATTCAGAATTTTATTAGAGAGAAGAAACTGGTGAAGGCTTATGGACGCATTCACAAATTCAAGTTATTAGACAAGTTCCCTCCAGCTCAAGTCTTAAAAGAATATGTTGAGAAGGCCATGAAGTGCATGGAAAATAGCGAGAGAAAAGACATGCTCCATCAACAGGATGAGGTTGTAGACGAAGGAATATCTGATCTACGACGTGCTATTGAATGCATCAAATATCACAACCTTGAGTCGGAATACCCTTCCGAGAGCATTGAGAAACAAATAGTTGTGCTACAGAAATTTATGGAGGATCGGAAAAAACAAAATCAGAAAAAACGCTTATGCAGCACAATTGATTCCAGCTTTCAACAGCAGGAACATAAATTTCAGCGGACATCCTTATCAGCGGGAAGACCATATGGATTGCACCCAGTCTATCCGAATCCAGGTTCATCATTTTACAATGGACAGTTTAGTATTCCTTCCAACAATCCCGTGCAGTTTAGCATGCCTGCCAACGGCGTTAATTATGAGTTCCCTAGAATATATCCATTCCCTCCATACCCTCTTGTATATAGACACCCTGGGTATGGCCTTCAAAAGCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

892

Amino Acids

103.52

Weight (kDa)

7.83

Isoelectric Point (pI)

53.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 458 - 612 4.6e-34 Frigida-like protein
Frigida PF07899 694 - 797 2e-18 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 781
Acc65I GGTACC 1 cut(s) 1823
AccB1I GGYRCC 1 cut(s) 1823
AccI GTMKAC 1 cut(s) 2256
AccII CGCG 1 cut(s) 1191
AciI CCGC 3 cut(s) 1711, 2458, 2474
AclWI GGATC 4 cut(s) 139, 277, 662, 2385
AcoI YGGCCR 1 cut(s) 1644
AcsI RAATTY 8 cut(s) 370, 450, 554, 1516, 2086, 2135, 2366, 2450
AcuI CTGAAG 1 cut(s) 95
AfaI GTAC 4 cut(s) 65, 1006, 1165, 1825
AfiI CCNNNNNNNGG 7 cut(s) 114, 1653, 2242, 2317, 2513, 2585, 2657
AflIII ACRYGT 2 cut(s) 682, 1565
AjiI CACGTC 1 cut(s) 2282
AjnI CCWGG 3 cut(s) 243, 2512, 2650
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 620, 652
Alw21I GWGCWC 2 cut(s) 180, 747
Alw26I GTCTC 5 cut(s) 439, 709, 1135, 1210, 1603
AlwI GGATC 4 cut(s) 139, 277, 662, 2385
AlwNI CAGNNNCTG 1 cut(s) 703
Ama87I CYCGRG 2 cut(s) 835, 1812
AoxI GGCC 4 cut(s) 267, 1644, 2193, 2659
ApeKI GCWGC 9 cut(s) 193, 1037, 1105, 1313, 1838, 1841, 2012, 2015, 2412
ApoI RAATTY 8 cut(s) 370, 450, 554, 1516, 2086, 2135, 2366, 2450
ArsI GACNNNNNNTTYG 1 cut(s) 36
AseI ATTAAT 1 cut(s) 1611
Asp700I GAANNNNTTC 2 cut(s) 1420, 1503
Asp718I GGTACC 1 cut(s) 1823
AspLEI GCGC 1 cut(s) 1606
AspS9I GGNCC 2 cut(s) 861, 954
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 6 cut(s) 97, 1438, 1499, 1633, 1661, 2122
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 2 cut(s) 835, 1812
AvaII GGWCC 2 cut(s) 861, 954
AxyI CCTNAGG 1 cut(s) 1617
BanI GGYRCC 1 cut(s) 1823
BanII GRGCYC 2 cut(s) 180, 1819
BauI CACGAG 2 cut(s) 504, 1896
BbsI GAAGAC 3 cut(s) 94, 1760, 2485
Bbv12I GWGCWC 2 cut(s) 180, 747
BbvI GCAGC 9 cut(s) 205, 1049, 1117, 1300, 1828, 1850, 2024, 2027, 2424
BccI CCATC 5 cut(s) 214, 523, 970, 1367, 2244
BceAI ACGGC 1 cut(s) 2602
BciT130I CCWGG 3 cut(s) 245, 2514, 2652
BclI TGATCA 2 cut(s) 387, 1348
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 5 cut(s) 439, 709, 1135, 1210, 1603
BfaI CTAG 4 cut(s) 98, 350, 809, 2607
BfmI CTRYAG 2 cut(s) 2010, 2360
Bme1390I CCNGG 4 cut(s) 149, 245, 2514, 2652
Bme18I GGWCC 2 cut(s) 861, 954
BmeT110I CYCGRG 2 cut(s) 835, 1812
BmgBI CACGTC 1 cut(s) 2282
BmgT120I GGNCC 2 cut(s) 861, 954
BmiI GGNNCC 3 cut(s) 424, 956, 1825
BmrFI CCNGG 4 cut(s) 149, 245, 2514, 2652
BmrI ACTGGG 1 cut(s) 2492
BmuI ACTGGG 1 cut(s) 2492
BpiI GAAGAC 3 cut(s) 94, 1760, 2485
BpmI CTGGAG 3 cut(s) 146, 362, 2145
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 3 cut(s) 764, 2151, 2333
BpuMI CCSGG 1 cut(s) 149
BsaJI CCNNGG 4 cut(s) 426, 1819, 2650, 2651
BsaXI ACNNNNNCTCC 4 cut(s) 719, 749, 845, 875
Bsc4I CCNNNNNNNGG 7 cut(s) 114, 1653, 2242, 2317, 2513, 2585, 2657
Bse118I RCCGGY 1 cut(s) 1646
Bse1I ACTGG 6 cut(s) 129, 286, 379, 1302, 2112, 2498
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 2 cut(s) 829, 1500
BseBI CCWGG 3 cut(s) 245, 2514, 2652
BseDI CCNNGG 4 cut(s) 426, 1819, 2650, 2651
BseGI GGATG 8 cut(s) 137, 157, 208, 769, 1378, 1495, 2251, 2462
BseLI CCNNNNNNNGG 7 cut(s) 114, 1653, 2242, 2317, 2513, 2585, 2657
BseMI GCAATG 2 cut(s) 829, 1500
BseMII CTCAG 4 cut(s) 695, 1455, 1631, 1671
BseNI ACTGG 6 cut(s) 129, 286, 379, 1302, 2112, 2498
BseRI GAGGAG 7 cut(s) 605, 662, 665, 725, 740, 743, 869
BseXI GCAGC 9 cut(s) 205, 1049, 1117, 1300, 1828, 1850, 2024, 2027, 2424
BseYI CCCAGC 2 cut(s) 1309, 2059
BsgI GTGCAG 2 cut(s) 1878, 2585
Bsh1236I CGCG 1 cut(s) 1191
BshFI GGCC 4 cut(s) 269, 1646, 2195, 2661
BshNI GGYRCC 1 cut(s) 1823
BsiHKAI GWGCWC 2 cut(s) 180, 747
BsiHKCI CYCGRG 2 cut(s) 835, 1812
BsiSI CCGG 2 cut(s) 148, 1647
BslFI GGGAC 2 cut(s) 1690, 2042
BslI CCNNNNNNNGG 7 cut(s) 114, 1653, 2242, 2317, 2513, 2585, 2657
BsmAI GTCTC 5 cut(s) 439, 709, 1135, 1210, 1603
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 2 cut(s) 1690, 2042
BsmI GAATGC 4 cut(s) 1118, 2002, 2127, 2297
BsnI GGCC 4 cut(s) 269, 1646, 2195, 2661
BsoBI CYCGRG 2 cut(s) 835, 1812
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 3 cut(s) 180, 747, 1819
Bsp143I GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
BspACI CCGC 3 cut(s) 1711, 2458, 2474
BspANI GGCC 4 cut(s) 269, 1646, 2195, 2661
BspCNI CTCAG 4 cut(s) 696, 1454, 1630, 1672
BspFNI CGCG 1 cut(s) 1191
BspLI GGNNCC 3 cut(s) 424, 956, 1825
BspMAI CTGCAG 1 cut(s) 2014
BspPI GGATC 4 cut(s) 139, 277, 662, 2385
BspQI GCTCTTC 3 cut(s) 168, 687, 1221
BspT104I TTCGAA 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 1823
BsrDI GCAATG 2 cut(s) 829, 1500
BsrFI RCCGGY 1 cut(s) 1646
BsrI ACTGG 6 cut(s) 129, 286, 379, 1302, 2112, 2498
BssAI RCCGGY 1 cut(s) 1646
BssECI CCNNGG 4 cut(s) 426, 1819, 2650, 2651
BssMI GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
BssSI CACGAG 2 cut(s) 504, 1896
BssT1I CCWWGG 2 cut(s) 426, 1819
Bst2BI CACGAG 2 cut(s) 504, 1896
Bst2UI CCWGG 3 cut(s) 245, 2514, 2652
Bst4CI ACNGT 5 cut(s) 50, 1525, 1672, 2056, 2538
Bst6I CTCTTC 7 cut(s) 168, 480, 618, 687, 1221, 1406, 1578
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 6 cut(s) 74, 1385, 1473, 1893, 2576, 2580
BstF5I GGATG 8 cut(s) 137, 157, 208, 769, 1378, 1495, 2251, 2462
BstFNI CGCG 1 cut(s) 1191
BstHHI GCGC 1 cut(s) 1606
BstKTI GATC 8 cut(s) 147, 285, 390, 670, 829, 1351, 2275, 2380
BstMAI GTCTC 5 cut(s) 439, 709, 1135, 1210, 1603
BstMBI GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
BstMWI GCNNNNNNNGC 4 cut(s) 1322, 1748, 2075, 2412
BstNI CCWGG 3 cut(s) 245, 2514, 2652
BstNSI RCATGY 3 cut(s) 1569, 2233, 2578
BstSCI CCNGG 4 cut(s) 147, 243, 2512, 2650
BstSFI CTRYAG 2 cut(s) 2010, 2360
BstUI CGCG 1 cut(s) 1191
BstV1I GCAGC 9 cut(s) 205, 1049, 1117, 1300, 1828, 1850, 2024, 2027, 2424
BstV2I GAAGAC 3 cut(s) 94, 1760, 2485
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
Bsu36I CCTNAGG 1 cut(s) 1617
BsuRI GGCC 4 cut(s) 269, 1646, 2195, 2661
BtrI CACGTC 1 cut(s) 2282
BtsCI GGATG 8 cut(s) 137, 157, 208, 769, 1378, 1495, 2251, 2462
BtsIMutI CAGTG 3 cut(s) 119, 1295, 2052
Cac8I GCNNGC 6 cut(s) 74, 1385, 1473, 1893, 2576, 2580
CaiI CAGNNNCTG 1 cut(s) 703
CfoI GCGC 1 cut(s) 1606
Cfr10I RCCGGY 1 cut(s) 1646
Cfr13I GGNCC 2 cut(s) 861, 954
CseI GACGC 1 cut(s) 2133
Csp6I GTAC 4 cut(s) 64, 1005, 1164, 1824
CviAII CATG 9 cut(s) 94, 182, 1396, 1566, 1790, 2197, 2206, 2230, 2575
CviQI GTAC 4 cut(s) 64, 1005, 1164, 1824
DpnI GATC 8 cut(s) 146, 284, 389, 669, 828, 1350, 2274, 2379
DpnII GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
DrdI GACNNNNNNGTC 1 cut(s) 781
DseDI GACNNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 1 cut(s) 1644
Eam1104I CTCTTC 7 cut(s) 168, 480, 618, 687, 1221, 1406, 1578
EarI CTCTTC 7 cut(s) 168, 480, 618, 687, 1221, 1406, 1578
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 426, 1819
Eco24I GRGCYC 2 cut(s) 180, 1819
Eco47I GGWCC 2 cut(s) 861, 954
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 95
Eco81I CCTNAGG 1 cut(s) 1617
Eco88I CYCGRG 2 cut(s) 835, 1812
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 954
EcoRI GAATTC 2 cut(s) 370, 450
EcoRII CCWGG 3 cut(s) 243, 2512, 2650
EcoT14I CCWWGG 2 cut(s) 426, 1819
EcoT22I ATGCAT 1 cut(s) 2297
EcoT38I GRGCYC 2 cut(s) 180, 1819
ErhI CCWWGG 2 cut(s) 426, 1819
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 9 cut(s) 97, 185, 1399, 1569, 1793, 2200, 2209, 2233, 2578
FalI AAGNNNNNCTT 4 cut(s) 350, 382, 1755, 1787
FaqI GGGAC 2 cut(s) 1690, 2042
FatI CATG 9 cut(s) 93, 181, 1395, 1565, 1789, 2196, 2205, 2229, 2574
FauI CCCGC 1 cut(s) 2467
FauNDI CATATG 3 cut(s) 1407, 1726, 2485
FbaI TGATCA 2 cut(s) 387, 1348
FblI GTMKAC 1 cut(s) 2256
FokI GGATG 8 cut(s) 144, 164, 215, 776, 1385, 1502, 2258, 2449
FriOI GRGCYC 2 cut(s) 180, 1819
FspBI CTAG 4 cut(s) 98, 350, 809, 2607
GlaI GCGC 1 cut(s) 1605
GsaI CCCAGC 2 cut(s) 1313, 2063
GsuI CTGGAG 3 cut(s) 146, 362, 2145
HaeIII GGCC 4 cut(s) 269, 1646, 2195, 2661
HapII CCGG 2 cut(s) 148, 1647
HgaI GACGC 1 cut(s) 2133
HhaI GCGC 1 cut(s) 1606
Hin1II CATG 9 cut(s) 97, 185, 1399, 1569, 1793, 2200, 2209, 2233, 2578
Hin6I GCGC 1 cut(s) 1604
HinP1I GCGC 1 cut(s) 1604
HpaII CCGG 2 cut(s) 148, 1647
HphI GGTGA 6 cut(s) 97, 1438, 1499, 1633, 1661, 2122
Hpy166II GTNNAC 2 cut(s) 1528, 2257
Hpy8I GTNNAC 2 cut(s) 1528, 2257
Hpy99I CGWCG 1 cut(s) 2283
HpyCH4III ACNGT 5 cut(s) 50, 1525, 1672, 2056, 2538
HpyCH4IV ACGT 4 cut(s) 682, 877, 1018, 2281
HpyF10VI GCNNNNNNNGC 4 cut(s) 1322, 1748, 2075, 2412
HpySE526I ACGT 4 cut(s) 682, 877, 1018, 2281
Hsp92II CATG 9 cut(s) 97, 185, 1399, 1569, 1793, 2200, 2209, 2233, 2578
HspAI GCGC 1 cut(s) 1604
KpnI GGTACC 1 cut(s) 1827
Ksp22I TGATCA 2 cut(s) 387, 1348
Kzo9I GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
LguI GCTCTTC 3 cut(s) 168, 687, 1221
LmnI GCTCC 7 cut(s) 183, 381, 422, 541, 1960, 1981, 2238
Lsp1109I GCAGC 9 cut(s) 205, 1049, 1117, 1300, 1828, 1850, 2024, 2027, 2424
MaeI CTAG 4 cut(s) 98, 350, 809, 2607
MaeII ACGT 4 cut(s) 682, 877, 1018, 2281
MaeIII GTNAC 3 cut(s) 685, 1717, 1960
MalI GATC 8 cut(s) 146, 284, 389, 669, 828, 1350, 2274, 2379
MboI GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
MfeI CAATTG 2 cut(s) 642, 2418
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 3 cut(s) 180, 747, 1819
MlyI GAGTC 7 cut(s) 162, 233, 306, 932, 1469, 1605, 2324
MmeI TCCRAC 5 cut(s) 180, 243, 1560, 2298, 2577
Mph1103I ATGCAT 1 cut(s) 2297
MroXI GAANNNNTTC 2 cut(s) 1420, 1503
MspA1I CMGCKG 5 cut(s) 1313, 1445, 1841, 2458, 2474
MspI CCGG 2 cut(s) 148, 1647
MspR9I CCNGG 4 cut(s) 149, 245, 2514, 2652
MunI CAATTG 2 cut(s) 642, 2418
Mva1269I GAATGC 4 cut(s) 1118, 2002, 2127, 2297
MvaI CCWGG 3 cut(s) 245, 2514, 2652
MvnI CGCG 1 cut(s) 1191
MwoI GCNNNNNNNGC 4 cut(s) 1322, 1748, 2075, 2412
NciI CCSGG 1 cut(s) 149
NdeI CATATG 3 cut(s) 1407, 1726, 2485
NdeII GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
NlaIII CATG 9 cut(s) 97, 185, 1399, 1569, 1793, 2200, 2209, 2233, 2578
NlaIV GGNNCC 3 cut(s) 424, 956, 1825
NmeAIII GCCGAG 1 cut(s) 1580
NmuCI GTSAC 1 cut(s) 1960
NsiI ATGCAT 1 cut(s) 2297
NspI RCATGY 3 cut(s) 1569, 2233, 2578
NspV TTCGAA 1 cut(s) 23
PaeI GCATGC 1 cut(s) 2578
PaeR7I CTCGAG 1 cut(s) 835
PasI CCCWGGG 1 cut(s) 2651
PciI ACATGT 1 cut(s) 1565
PciSI GCTCTTC 3 cut(s) 168, 687, 1221
PctI GAATGC 4 cut(s) 1118, 2002, 2127, 2297
PdmI GAANNNNTTC 2 cut(s) 1420, 1503
PfoI TCCNGGA 2 cut(s) 147, 243
PleI GAGTC 7 cut(s) 162, 232, 306, 932, 1468, 1604, 2323
PpsI GAGTC 7 cut(s) 162, 232, 306, 932, 1468, 1604, 2323
PpuMI RGGWCCY 1 cut(s) 954
PscI ACATGT 1 cut(s) 1565
PshBI ATTAAT 1 cut(s) 1611
Psp124BI GAGCTC 1 cut(s) 180
Psp5II RGGWCCY 1 cut(s) 954
Psp6I CCWGG 3 cut(s) 243, 2512, 2650
PspFI CCCAGC 2 cut(s) 1309, 2059
PspGI CCWGG 3 cut(s) 243, 2512, 2650
PspN4I GGNNCC 3 cut(s) 424, 956, 1825
PspPI GGNCC 2 cut(s) 861, 954
PspPPI RGGWCCY 1 cut(s) 954
PstI CTGCAG 1 cut(s) 2014
PstNI CAGNNNCTG 1 cut(s) 703
PsuI RGATCY 1 cut(s) 282
PvuII CAGCTG 3 cut(s) 1313, 1445, 1841
RsaI GTAC 4 cut(s) 65, 1006, 1165, 1825
RsaNI GTAC 4 cut(s) 64, 1005, 1164, 1824
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 3 cut(s) 168, 687, 1221
Sau3AI GATC 8 cut(s) 144, 282, 387, 667, 826, 1348, 2272, 2377
Sau96I GGNCC 2 cut(s) 861, 954
SchI GAGTC 7 cut(s) 162, 233, 306, 932, 1469, 1605, 2324
ScrFI CCNGG 4 cut(s) 149, 245, 2514, 2652
SduI GDGCHC 3 cut(s) 180, 747, 1819
SfcI CTRYAG 2 cut(s) 2010, 2360
Sfr274I CTCGAG 1 cut(s) 835
SfuI TTCGAA 1 cut(s) 23
SinI GGWCC 2 cut(s) 861, 954
SlaI CTCGAG 1 cut(s) 835
SmlI CTYRAG 4 cut(s) 779, 835, 2166, 2312
SmoI CTYRAG 4 cut(s) 779, 835, 2166, 2312
SphI GCATGC 1 cut(s) 2578
SsiI CCGC 3 cut(s) 1711, 2458, 2474
SspMI CTAG 4 cut(s) 98, 350, 809, 2607
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 4 cut(s) 147, 243, 2512, 2650
StyI CCWWGG 2 cut(s) 426, 1819
TaaI ACNGT 5 cut(s) 50, 1525, 1672, 2056, 2538
TaiI ACGT 4 cut(s) 685, 880, 1021, 2284
TauI GCSGC 1 cut(s) 1714
TscAI CASTG 3 cut(s) 119, 1302, 2059
TseFI GTSAC 1 cut(s) 1960
TseI GCWGC 9 cut(s) 193, 1037, 1105, 1313, 1838, 1841, 2012, 2015, 2412
Tsp45I GTSAC 1 cut(s) 1960
TspDTI ATGAA 9 cut(s) 198, 1292, 1326, 1384, 1424, 1743, 1806, 2213, 2508
TspRI CASTG 3 cut(s) 119, 1302, 2059
VpaK11BI GGWCC 2 cut(s) 861, 954
VspI ATTAAT 1 cut(s) 1611
XapI RAATTY 8 cut(s) 370, 450, 554, 1516, 2086, 2135, 2366, 2450
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 3 cut(s) 1569, 2233, 2578
XcmI CCANNNNNNNNNTGG 1 cut(s) 2203
XhoI CTCGAG 1 cut(s) 835
XmiI GTMKAC 1 cut(s) 2256
XmnI GAANNNNTTC 2 cut(s) 1420, 1503
XspI CTAG 4 cut(s) 98, 350, 809, 2607
Zsp2I ATGCAT 1 cut(s) 2297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.