RLG00000028031

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016117
Physical Location & Seq
Forward (+)
17876658 .. 17878058
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028031

Sequence Viewer

Length: 750 bp
ATGGCAAACATTTTCTCCTATGCTTCCATTACACCACGCCTCACTGCTCAGCACCACTACCTCAGTTTCCAGCCAAATCATCTCAATTTACAGCCAAGTCAGCGTTACAGTGTGTGCAGAATTCGTTGCAGTGGTGAAAACCCAACAAGCAAATCAACATCCAATACAGAATCCCAACCAGAAAATGGAAATGTAGTGCTTAAGGTTGCTTGGTACGCCTCTGAGTTTTTGGGCGTTGCCACTTCGTTTTTCAACCCACCGTCCAAAGTTGAAGCTCCTGAAACTGCTCTTGAGCTTGCAAGAGATGGGTCTGGAGCCGTTGATCGTGCCGGAGTAGTAAAATCAATCAAAGATGACTTTGAAAGATCATACTTTGTCACAGGGAACCTTACGCTTGATGCATATGAAGAGGATTGTGAATTTGCTGATCCTGCAAGCTCATTCAAAGGGCTTCAACGTTTCAAAAGGAACTGTACTAATTTTGGATCCCTTTTAAAGAAGTCAAATATGAAGCTTATGAAGTGGGAAGATTTTGAGGATAAAGGAATTGGTCACTGGAGATTCAGTTGTATCTTATCATTCCCTTGGAGGCCAATTCTATCTGCAAGTGGATACACTGAGTACTACTTTGATGAACGGTCAGGGAAAGTTTCCAGGCATGTAGAGCACTGGAATGTTCCCAAAATGGTTTTACTGAAGCAGATTTTAAAGCCAAGCAGGGGATTCTGGCTTAAGAAAACAGGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

250

Amino Acids

28.27

Weight (kDa)

9.01

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 112 - 220 1.1e-12 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46100 AT2G46100
fragaria_vesca FvH4_7g15085
malus_domestica MD01G1069500.v1.1
prunus_persica Prupe.2G175600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357281
rosa_laevigata RLG00000028031
rosa_rugosa Rorug01G0256000
rosa_samantha Rh1AG269300 Rh1BG237200 Rh1CG253500 Rh1DG265100
rosa_wichuraiana Rw1G023850 Rw1G024010 Rw1G024050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 185
AclI AACGTT 1 cut(s) 457
AclWI GGATC 3 cut(s) 422, 480, 493
AcsI RAATTY 2 cut(s) 120, 419
AcuI CTGAAG 1 cut(s) 716
AfaI GTAC 3 cut(s) 215, 475, 623
AfiI CCNNNNNNNGG 2 cut(s) 185, 719
AflII CTTAAG 2 cut(s) 200, 731
AgsI TTSAA 6 cut(s) 253, 272, 362, 445, 455, 463
AjnI CCWGG 1 cut(s) 653
AluBI AGCT 4 cut(s) 275, 295, 438, 514
AluI AGCT 4 cut(s) 275, 295, 438, 514
Alw21I GWGCWC 1 cut(s) 669
AlwI GGATC 3 cut(s) 422, 480, 493
AoxI GGCC 1 cut(s) 590
ApoI RAATTY 2 cut(s) 120, 419
AsuHPI GGTGA 1 cut(s) 146
BamHI GGATCC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 669
BccI CCATC 1 cut(s) 299
BceAI ACGGC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 655
BciVI GTATCC 1 cut(s) 605
BfrI CTTAAG 2 cut(s) 200, 731
BfuI GTATCC 1 cut(s) 605
BlpI GCTNAGC 1 cut(s) 48
BmcAI AGTACT 1 cut(s) 623
Bme1390I CCNGG 1 cut(s) 655
BmiI GGNNCC 3 cut(s) 316, 386, 487
BmrFI CCNGG 1 cut(s) 655
BmsI GCATC 1 cut(s) 388
BpmI CTGGAG 2 cut(s) 333, 577
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 584
BsaXI ACNNNNNCTCC 3 cut(s) 29, 550, 580
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 719
Bse1I ACTGG 2 cut(s) 560, 674
BseBI CCWGG 1 cut(s) 655
BseDI CCNNGG 1 cut(s) 584
BseGI GGATG 1 cut(s) 158
BseLI CCNNNNNNNGG 2 cut(s) 185, 719
BseMII CTCAG 4 cut(s) 62, 76, 213, 609
BseNI ACTGG 2 cut(s) 560, 674
BsgI GTGCAG 1 cut(s) 136
BshFI GGCC 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 669
BsiSI CCGG 1 cut(s) 330
BslI CCNNNNNNNGG 2 cut(s) 185, 719
BsnI GGCC 1 cut(s) 592
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 4 cut(s) 322, 365, 427, 485
Bsp1720I GCTNAGC 1 cut(s) 48
BspANI GGCC 1 cut(s) 592
BspCNI CTCAG 4 cut(s) 61, 75, 214, 610
BspLI GGNNCC 3 cut(s) 316, 386, 487
BspPI GGATC 3 cut(s) 422, 480, 493
BspTI CTTAAG 2 cut(s) 200, 731
BsrI ACTGG 2 cut(s) 560, 674
BssECI CCNNGG 1 cut(s) 584
BssMI GATC 4 cut(s) 322, 365, 427, 485
BssT1I CCWWGG 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 655
Bst4CI ACNGT 4 cut(s) 110, 261, 473, 639
Bst6I CTCTTC 1 cut(s) 402
BstAFI CTTAAG 2 cut(s) 200, 731
BstC8I GCNNGC 2 cut(s) 297, 436
BstDEI CTNAG 4 cut(s) 48, 62, 222, 618
BstF5I GGATG 1 cut(s) 158
BstKTI GATC 4 cut(s) 325, 368, 430, 488
BstMBI GATC 4 cut(s) 322, 365, 427, 485
BstMWI GCNNNNNNNGC 4 cut(s) 100, 215, 431, 664
BstNI CCWGG 1 cut(s) 655
BstNSI RCATGY 1 cut(s) 662
BstSCI CCNGG 1 cut(s) 653
BstX2I RGATCY 1 cut(s) 485
BstYI RGATCY 1 cut(s) 485
BsuI GTATCC 1 cut(s) 605
BsuRI GGCC 1 cut(s) 592
BtsCI GGATG 1 cut(s) 158
BtsI GCAGTG 2 cut(s) 42, 136
BtsIMutI CAGTG 6 cut(s) 42, 115, 136, 553, 615, 667
Cac8I GCNNGC 2 cut(s) 297, 436
Csp6I GTAC 3 cut(s) 214, 474, 622
CviAII CATG 1 cut(s) 659
CviQI GTAC 3 cut(s) 214, 474, 622
DdeI CTNAG 4 cut(s) 48, 62, 222, 618
DpnI GATC 4 cut(s) 324, 367, 429, 487
DpnII GATC 4 cut(s) 322, 365, 427, 485
DraI TTTAAA 2 cut(s) 495, 708
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 716
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 653
EcoT14I CCWWGG 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 1 cut(s) 584
FaeI CATG 1 cut(s) 662
FaiI YATR 7 cut(s) 21, 370, 403, 405, 509, 518, 660
FatI CATG 1 cut(s) 658
FauNDI CATATG 1 cut(s) 403
FokI GGATG 1 cut(s) 145
GsuI CTGGAG 2 cut(s) 333, 577
HaeIII GGCC 1 cut(s) 592
HapII CCGG 1 cut(s) 330
Hin1II CATG 1 cut(s) 662
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 3 cut(s) 170, 561, 723
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 146
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 3 cut(s) 278, 290, 312
HpyCH4III ACNGT 4 cut(s) 110, 261, 473, 639
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 6 cut(s) 117, 129, 299, 401, 434, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 215, 431, 664
HpyF3I CTNAG 4 cut(s) 48, 62, 222, 618
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 1 cut(s) 662
Kzo9I GATC 4 cut(s) 322, 365, 427, 485
LmnI GCTCC 2 cut(s) 280, 314
LweI GCATC 1 cut(s) 388
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 3 cut(s) 104, 376, 551
MalI GATC 4 cut(s) 324, 367, 429, 487
MboI GATC 4 cut(s) 322, 365, 427, 485
MboII GAAGA 2 cut(s) 419, 539
MflI RGATCY 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 6 cut(s) 85, 120, 419, 478, 546, 594
MnlI CCTC 6 cut(s) 50, 71, 229, 403, 529, 582
Mph1103I ATGCAT 1 cut(s) 403
MseI TTAA 4 cut(s) 201, 494, 707, 732
MslI CAYNNNNRTG 1 cut(s) 672
MspCI CTTAAG 2 cut(s) 200, 731
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 655
MvaI CCWGG 1 cut(s) 655
MwoI GCNNNNNNNGC 4 cut(s) 100, 215, 431, 664
NdeI CATATG 1 cut(s) 403
NdeII GATC 4 cut(s) 322, 365, 427, 485
NlaIII CATG 1 cut(s) 662
NlaIV GGNNCC 3 cut(s) 316, 386, 487
NmuCI GTSAC 2 cut(s) 376, 551
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 1 cut(s) 662
PfeI GAWTC 3 cut(s) 170, 561, 723
PflMI CCANNNNNTGG 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 457
Psp6I CCWGG 1 cut(s) 653
PspGI CCWGG 1 cut(s) 653
PspN4I GGNNCC 3 cut(s) 316, 386, 487
PsuI RGATCY 1 cut(s) 485
RsaI GTAC 3 cut(s) 215, 475, 623
RsaNI GTAC 3 cut(s) 214, 474, 622
RseI CAYNNNNRTG 1 cut(s) 672
SaqAI TTAA 4 cut(s) 201, 494, 707, 732
Sau3AI GATC 4 cut(s) 322, 365, 427, 485
ScaI AGTACT 1 cut(s) 623
ScrFI CCNGG 1 cut(s) 655
SduI GDGCHC 1 cut(s) 669
SetI ASST 9 cut(s) 63, 207, 277, 297, 390, 440, 460, 516, 745
SfaNI GCATC 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 672
SmlI CTYRAG 3 cut(s) 200, 290, 731
SmoI CTYRAG 3 cut(s) 200, 290, 731
Sse9I AATT 6 cut(s) 85, 120, 419, 478, 546, 594
StyD4I CCNGG 1 cut(s) 653
StyI CCWWGG 1 cut(s) 584
TaaI ACNGT 4 cut(s) 110, 261, 473, 639
TaiI ACGT 1 cut(s) 460
TasI AATT 6 cut(s) 85, 120, 419, 478, 546, 594
TatI WGTACW 2 cut(s) 473, 621
TfiI GAWTC 3 cut(s) 170, 561, 723
Tru1I TTAA 4 cut(s) 201, 494, 707, 732
Tru9I TTAA 4 cut(s) 201, 494, 707, 732
TscAI CASTG 6 cut(s) 49, 115, 136, 560, 622, 674
TseFI GTSAC 2 cut(s) 376, 551
Tsp45I GTSAC 2 cut(s) 376, 551
TspDTI ATGAA 4 cut(s) 420, 524, 533, 648
TspRI CASTG 6 cut(s) 49, 115, 136, 560, 622, 674
Van91I CCANNNNNTGG 1 cut(s) 185
Vha464I CTTAAG 2 cut(s) 200, 731
XapI RAATTY 2 cut(s) 120, 419
XceI RCATGY 1 cut(s) 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
ZrmI AGTACT 1 cut(s) 623
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.