Rh1AG269300

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0016117
Physical Location & Seq
Reverse (-)
49701299 .. 49770887
69589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG269300.1

Sequence Viewer

Length: 648 bp
ATGGCAAACCTTCTCTCCTATGCTTCCATTACACCACGTCTCACCGCTCAGCACCACCACCTCAGTTTCCAGCCAAATCATCTCAGTTTACAGCCAAATCAGCGTTACAGTGTCTGCAGATTTCGTTGCAGTGGAGAAAACCCAACAAGGAAATCAACATCCAATACAGAATCCCAACCAGAAAATGCAAATGTAGTGCTTAAGGTTGCTTGGTATGCCTCTGAGTTTTTGGGCATTGCCACTTCGTTTTTCAACCCACCATCCAAAGTTGAAGCTCCTGAAACTGCTCTTGAGCTTGCAAGAGATGGGTCTGGAGCCGTTGATCGTGCCGGAGTAGTAAAATCAATCAAAGATGACTTTGAAAGATCTTACTTTGTCACAGGGAACCTTACGCTTGATGCATATGAAGAGGATTGTGAATTTGCTGATCCTGCAAGCTCATTCAAAGGGCTTCAACGTGTCAAAAGGAACTGTACTAATTTTGGATCCCTTTTAAAGAAGTCAAATATGAAGCTTATGAAGTGGGAAGATTTTGAGGATAAAGGAATTGGTCACTGGAGATTCAGTTGTATCTTGTCATTCCCTTGGAGGCCAATTCTGTCTGGTATGCTTTTGTGCAATCACTTGTTAACTACATGTATCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

215

Amino Acids

24.23

Weight (kDa)

8.46

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 112 - 199 2.8e-07 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46100 AT2G46100
fragaria_vesca FvH4_7g15085
malus_domestica MD01G1069500.v1.1
prunus_persica Prupe.2G175600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357281
rosa_laevigata RLG00000028031
rosa_rugosa Rorug01G0256000
rosa_samantha Rh1AG269300 Rh1BG237200 Rh1CG253500 Rh1DG265100
rosa_wichuraiana Rw1G023850 Rw1G024010 Rw1G024050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 47
AciI CCGC 1 cut(s) 45
AclWI GGATC 3 cut(s) 422, 480, 493
AcsI RAATTY 1 cut(s) 419
AfaI GTAC 1 cut(s) 475
AflII CTTAAG 1 cut(s) 200
AflIII ACRYGT 2 cut(s) 457, 635
AgsI TTSAA 5 cut(s) 253, 272, 362, 445, 455
AjiI CACGTC 1 cut(s) 38
AluBI AGCT 4 cut(s) 275, 295, 438, 514
AluI AGCT 4 cut(s) 275, 295, 438, 514
Alw26I GTCTC 1 cut(s) 44
AlwI GGATC 3 cut(s) 422, 480, 493
AlwNI CAGNNNCTG 1 cut(s) 114
AoxI GGCC 1 cut(s) 590
ApoI RAATTY 1 cut(s) 419
AsuHPI GGTGA 1 cut(s) 34
BamHI GGATCC 1 cut(s) 485
BccI CCATC 2 cut(s) 268, 299
BceAI ACGGC 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 115
BfrI CTTAAG 1 cut(s) 200
BglII AGATCT 1 cut(s) 365
BlpI GCTNAGC 1 cut(s) 48
BmgBI CACGTC 1 cut(s) 38
BmiI GGNNCC 3 cut(s) 316, 386, 487
BmsI GCATC 1 cut(s) 388
BpmI CTGGAG 2 cut(s) 333, 577
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 584
BsaXI ACNNNNNCTCC 3 cut(s) 29, 550, 580
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 584
BseGI GGATG 2 cut(s) 158, 260
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 4 cut(s) 62, 76, 97, 213
BseNI ACTGG 1 cut(s) 560
BshFI GGCC 1 cut(s) 592
BsiSI CCGG 1 cut(s) 330
BsmAI GTCTC 1 cut(s) 44
BsmBI CGTCTC 1 cut(s) 44
BsnI GGCC 1 cut(s) 592
Bsp143I GATC 4 cut(s) 322, 365, 427, 485
Bsp1720I GCTNAGC 1 cut(s) 48
BspACI CCGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 592
BspCNI CTCAG 4 cut(s) 61, 75, 96, 214
BspLI GGNNCC 3 cut(s) 316, 386, 487
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 3 cut(s) 422, 480, 493
BspTI CTTAAG 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 1 cut(s) 584
BssMI GATC 4 cut(s) 322, 365, 427, 485
BssT1I CCWWGG 1 cut(s) 584
Bst4CI ACNGT 2 cut(s) 110, 473
Bst6I CTCTTC 1 cut(s) 402
BstAFI CTTAAG 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 297, 436
BstDEI CTNAG 4 cut(s) 48, 62, 83, 222
BstF5I GGATG 2 cut(s) 158, 260
BstKTI GATC 4 cut(s) 325, 368, 430, 488
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 4 cut(s) 322, 365, 427, 485
BstMWI GCNNNNNNNGC 3 cut(s) 100, 215, 431
BstNSI RCATGY 1 cut(s) 639
BstSFI CTRYAG 1 cut(s) 115
BstX2I RGATCY 2 cut(s) 365, 485
BstYI RGATCY 2 cut(s) 365, 485
BsuRI GGCC 1 cut(s) 592
BtrI CACGTC 1 cut(s) 38
BtsCI GGATG 2 cut(s) 158, 260
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 3 cut(s) 115, 136, 553
Cac8I GCNNGC 2 cut(s) 297, 436
CaiI CAGNNNCTG 1 cut(s) 114
Csp6I GTAC 1 cut(s) 474
CviAII CATG 1 cut(s) 636
CviJI RGCY 9 cut(s) 73, 94, 275, 295, 317, 438, 451, 514, 592
CviKI_1 RGCY 9 cut(s) 73, 94, 275, 295, 317, 438, 451, 514, 592
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 4 cut(s) 48, 62, 83, 222
DpnI GATC 4 cut(s) 324, 367, 429, 487
DpnII GATC 4 cut(s) 322, 365, 427, 485
DraI TTTAAA 1 cut(s) 495
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 584
EcoT14I CCWWGG 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 1 cut(s) 584
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 1 cut(s) 639
FaiI YATR 8 cut(s) 21, 216, 403, 405, 509, 518, 608, 637
FatI CATG 1 cut(s) 635
FauNDI CATATG 1 cut(s) 403
FokI GGATG 2 cut(s) 145, 247
GsuI CTGGAG 2 cut(s) 333, 577
HaeIII GGCC 1 cut(s) 592
HapII CCGG 1 cut(s) 330
Hin1II CATG 1 cut(s) 639
HincII GTYRAC 1 cut(s) 630
HindII GTYRAC 1 cut(s) 630
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 2 cut(s) 170, 561
HpaI GTTAAC 1 cut(s) 630
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 89, 630
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 3 cut(s) 278, 290, 312
Hpy8I GTNNAC 2 cut(s) 89, 630
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 2 cut(s) 110, 473
HpyCH4IV ACGT 2 cut(s) 37, 457
HpyCH4V TGCA 7 cut(s) 117, 129, 188, 299, 401, 434, 618
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 215, 431
HpyF3I CTNAG 4 cut(s) 48, 62, 83, 222
HpySE526I ACGT 2 cut(s) 37, 457
Hsp92II CATG 1 cut(s) 639
KspAI GTTAAC 1 cut(s) 630
Kzo9I GATC 4 cut(s) 322, 365, 427, 485
LmnI GCTCC 2 cut(s) 280, 314
LpnPI CCDG 9 cut(s) 83, 192, 291, 297, 343, 366, 444, 541, 588
LweI GCATC 1 cut(s) 388
MaeII ACGT 2 cut(s) 37, 457
MaeIII GTNAC 3 cut(s) 104, 376, 551
MalI GATC 4 cut(s) 324, 367, 429, 487
MbiI CCGCTC 1 cut(s) 47
MboI GATC 4 cut(s) 322, 365, 427, 485
MboII GAAGA 2 cut(s) 419, 539
MflI RGATCY 2 cut(s) 365, 485
MluCI AATT 4 cut(s) 419, 478, 546, 594
MnlI CCTC 5 cut(s) 71, 229, 403, 529, 582
Mph1103I ATGCAT 1 cut(s) 403
MseI TTAA 3 cut(s) 201, 494, 629
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 100, 215, 431
NdeI CATATG 1 cut(s) 403
NdeII GATC 4 cut(s) 322, 365, 427, 485
NlaIII CATG 1 cut(s) 639
NlaIV GGNNCC 3 cut(s) 316, 386, 487
NmuCI GTSAC 2 cut(s) 376, 551
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 1 cut(s) 639
PciI ACATGT 1 cut(s) 635
PfeI GAWTC 2 cut(s) 170, 561
PscI ACATGT 1 cut(s) 635
PspN4I GGNNCC 3 cut(s) 316, 386, 487
PstI CTGCAG 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 114
PsuI RGATCY 2 cut(s) 365, 485
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
SaqAI TTAA 3 cut(s) 201, 494, 629
Sau3AI GATC 4 cut(s) 322, 365, 427, 485
SfaNI GCATC 1 cut(s) 388
SfcI CTRYAG 1 cut(s) 115
SmlI CTYRAG 2 cut(s) 200, 290
SmoI CTYRAG 2 cut(s) 200, 290
Sse9I AATT 4 cut(s) 419, 478, 546, 594
SsiI CCGC 1 cut(s) 45
StyI CCWWGG 1 cut(s) 584
TaaI ACNGT 2 cut(s) 110, 473
TaiI ACGT 2 cut(s) 40, 460
TasI AATT 4 cut(s) 419, 478, 546, 594
TatI WGTACW 1 cut(s) 473
TfiI GAWTC 2 cut(s) 170, 561
Tru1I TTAA 3 cut(s) 201, 494, 629
Tru9I TTAA 3 cut(s) 201, 494, 629
TscAI CASTG 3 cut(s) 115, 136, 560
TseFI GTSAC 2 cut(s) 376, 551
Tsp45I GTSAC 2 cut(s) 376, 551
TspDTI ATGAA 3 cut(s) 420, 524, 533
TspRI CASTG 3 cut(s) 115, 136, 560
Vha464I CTTAAG 1 cut(s) 200
XapI RAATTY 1 cut(s) 419
XceI RCATGY 1 cut(s) 639
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.