RLG00000028747

Palmitoyl-protein thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013416
Physical Location & Seq
Forward (+)
27402578 .. 27407763
5186 bp
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UTR
Exon/CDS
Intron
RLM00000028747

Sequence Viewer

Length: 984 bp
ATGGCTTTTCCACTGTTACCGATCATTTTCATCTTCTTCTTCACCATCACTCTCATCCCACTTACATTCTCTGTTCCTTTCATTGTTTTTCATGGCATTGGAGATAAATGCAGCAATCGGGGAGTCACACACTTTACGGAGCTCTTAAGCAAATGGTCTGGTTCTCAAGGATATTGCGTAGAAATTGGGGATGGATCATGGGATTCCTGGACAATGCCTTTACTGGAACAGACGGCTATTGCTTGTGAGAAGGTTAAAAATATGAGTGAATTAAGTGACGGTTACAACATAGTTGGACTTTCTCAGGGTAGCATGGTTGGTAGAGGAGTAATTGAATTTTGTGATGGTGGACCTCCTGTCAGAAATTTTGTATCACTAGCAGGTCCTCACGCCGGTACCGCTTCTATTCCATTTTGTGGATCCGAGTGGCTTTGTATTCTTCTGGACGACTTAATCAAGTCAGAGATCTACAGCAGCTTTATCCAGGAACATTTGGCTCCAAGTGGTTATGTTAAAATCCCTACTGATATTGCTGCCTATCTAAAAGGGTGTACATTTCTTCCAAAGCTGAACAATGAAATCAATTTTACGAGAAATTCCACATATAAGGAACGATTTGCAAGCTTAGAGAATTTGGTGCTTATTATGTTTGAGCAAGACACCATATTGATACCCAAGGAGACCTCCTGGTTTGGATATTTCCCAGATGGGGCCTTTGATCCTATATTGTCTGCACAGGAGACCGAGCTGTACATTGAAGACTGGATTGGTTTGAAAACCTTGGACGAAGCTGGTAAAGTCAAGTTCATAAACGTGTCAGGTAATCATCTGGCCATCACGAGAAGTGACATGAAAAAGTATGTTGTGCCGTACTTGGAAGATGGAACATCAATGCAAACGATGATAGCAGAGCCCTCATCACAAAAATTACTTTCATCAGCTTGGAACTTCCTCTTGGAATTTGCTGGGCTCAAAGAGGATTGA

Protein Analysis

328

Amino Acids

36.38

Weight (kDa)

4.76

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Palm_thioest PF02089 26 - 276 2.3e-75 Palmitoyl protein thioesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013416)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60340 AT3G60340
fragaria_vesca FvH4_7g09920
malus_domestica MD07G1104400.v1.1
prunus_persica Prupe.2G128000_v2.0.a1 Prupe.2G128000_v2.0.a1
pyrus_communis pycom07g08940
rosa_chinensis RchiOBHm_Chr1g0347441
rosa_laevigata RLG00000028747
rosa_multiflora Rmu_sc0009756.1_g000009
rosa_roxburghii Rroxscaffold_4G00307770
rosa_rugosa Rorug01G0186900 Rorug01G0187000
rosa_samantha Rh1AG204400 Rh1BG170100 Rh1CG189500 Rh1DG200500
rosa_wichuraiana Rw1G017150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 371
Acc65I GGTACC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 416
AciI CCGC 1 cut(s) 399
AclWI GGATC 4 cut(s) 202, 414, 427, 713
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 5 cut(s) 335, 364, 595, 631, 959
AfaI GTAC 4 cut(s) 397, 553, 752, 872
AfiI CCNNNNNNNGG 3 cut(s) 392, 416, 709
AflII CTTAAG 1 cut(s) 145
AflIII ACRYGT 1 cut(s) 813
AgsI TTSAA 3 cut(s) 335, 758, 775
AhdI GACNNNNNGTC 1 cut(s) 356
AjnI CCWGG 3 cut(s) 206, 483, 686
AjuI GAANNNNNNNTTGG 4 cut(s) 750, 782, 938, 970
AluBI AGCT 7 cut(s) 142, 477, 568, 624, 748, 791, 941
AluI AGCT 7 cut(s) 142, 477, 568, 624, 748, 791, 941
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 674, 734
AlwI GGATC 4 cut(s) 202, 414, 427, 713
AoxI GGCC 2 cut(s) 711, 831
ApeKI GCWGC 3 cut(s) 111, 474, 533
ApoI RAATTY 5 cut(s) 335, 364, 595, 631, 959
Asp718I GGTACC 1 cut(s) 395
AspS9I GGNCC 3 cut(s) 350, 383, 711
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 2 cut(s) 350, 383
BaeI ACNNNNGTAYC 2 cut(s) 379, 412
BalI TGGCCA 1 cut(s) 833
BamHI GGATCC 1 cut(s) 419
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 3 cut(s) 144, 915, 972
BauI CACGAG 1 cut(s) 838
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 3 cut(s) 123, 486, 520
BccI CCATC 6 cut(s) 53, 185, 338, 701, 842, 875
BceAI ACGGC 2 cut(s) 249, 853
BciT130I CCWGG 3 cut(s) 208, 485, 688
BcoDI GTCTC 2 cut(s) 674, 734
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 469
BfrI CTTAAG 1 cut(s) 145
BfuAI ACCTGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 465
BisI GCNGC 3 cut(s) 112, 475, 534
BlsI GCNGC 3 cut(s) 113, 476, 535
Bme1390I CCNGG 3 cut(s) 208, 485, 688
Bme18I GGWCC 2 cut(s) 350, 383
BmeRI GACNNNNNGTC 1 cut(s) 356
BmgT120I GGNCC 3 cut(s) 350, 383, 711
BmiI GGNNCC 4 cut(s) 397, 421, 498, 712
BmrFI CCNGG 3 cut(s) 208, 485, 688
BpiI GAAGAC 1 cut(s) 765
BpuEI CTTGAG 1 cut(s) 150
BsaI GGTCTC 2 cut(s) 674, 734
BsaJI CCNNGG 2 cut(s) 675, 780
Bsc4I CCNNNNNNNGG 3 cut(s) 392, 416, 709
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 228, 767
BseBI CCWGG 3 cut(s) 208, 485, 688
BseDI CCNNGG 2 cut(s) 675, 780
BseGI GGATG 2 cut(s) 54, 196
BseLI CCNNNNNNNGG 3 cut(s) 392, 416, 709
BseMII CTCAG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 228, 767
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 3 cut(s) 123, 486, 520
BseYI CCCAGC 1 cut(s) 965
BsgI GTGCAG 1 cut(s) 717
BshFI GGCC 2 cut(s) 713, 833
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 393
BslI CCNNNNNNNGG 3 cut(s) 392, 416, 709
BsmAI GTCTC 2 cut(s) 674, 734
BsnI GGCC 2 cut(s) 713, 833
Bso31I GGTCTC 2 cut(s) 674, 734
Bsp1286I GDGCHC 3 cut(s) 144, 915, 972
Bsp1407I TGTACA 2 cut(s) 551, 750
Bsp143I GATC 5 cut(s) 21, 194, 419, 465, 718
BspACI CCGC 1 cut(s) 399
BspANI GGCC 2 cut(s) 713, 833
BspCNI CTCAG 1 cut(s) 316
BspLI GGNNCC 4 cut(s) 397, 421, 498, 712
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 4 cut(s) 202, 414, 427, 713
BspT107I GGYRCC 1 cut(s) 395
BspTI CTTAAG 1 cut(s) 145
BspTNI GGTCTC 2 cut(s) 674, 734
BsrFI RCCGGY 1 cut(s) 392
BsrGI TGTACA 2 cut(s) 551, 750
BsrI ACTGG 2 cut(s) 228, 767
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 675, 780
BssMI GATC 5 cut(s) 21, 194, 419, 465, 718
BssSI CACGAG 1 cut(s) 838
BssT1I CCWWGG 2 cut(s) 675, 780
Bst2BI CACGAG 1 cut(s) 838
Bst2UI CCWGG 3 cut(s) 208, 485, 688
Bst4CI ACNGT 2 cut(s) 15, 281
BstAFI CTTAAG 1 cut(s) 145
BstAUI TGTACA 2 cut(s) 551, 750
BstC8I GCNNGC 1 cut(s) 622
BstDEI CTNAG 2 cut(s) 303, 625
BstF5I GGATG 2 cut(s) 54, 196
BstKTI GATC 5 cut(s) 24, 197, 422, 468, 721
BstMAI GTCTC 2 cut(s) 674, 734
BstMBI GATC 5 cut(s) 21, 194, 419, 465, 718
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNI CCWGG 3 cut(s) 208, 485, 688
BstSCI CCNGG 3 cut(s) 206, 483, 686
BstSFI CTRYAG 1 cut(s) 469
BstV1I GCAGC 3 cut(s) 123, 486, 520
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 2 cut(s) 419, 465
BstYI RGATCY 2 cut(s) 419, 465
BsuRI GGCC 2 cut(s) 713, 833
BtsCI GGATG 2 cut(s) 54, 196
BtsIMutI CAGTG 1 cut(s) 11
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 3 cut(s) 350, 383, 711
Csp6I GTAC 4 cut(s) 396, 552, 751, 871
CviAII CATG 4 cut(s) 92, 198, 313, 850
CviQI GTAC 4 cut(s) 396, 552, 751, 871
DdeI CTNAG 2 cut(s) 303, 625
DpnI GATC 5 cut(s) 23, 196, 421, 467, 720
DpnII GATC 5 cut(s) 21, 194, 419, 465, 718
DriI GACNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 1 cut(s) 831
Eam1105I GACNNNNNGTC 1 cut(s) 356
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 675, 780
Eco24I GRGCYC 3 cut(s) 144, 915, 972
Eco31I GGTCTC 2 cut(s) 674, 734
Eco47I GGWCC 2 cut(s) 350, 383
Eco53kI GAGCTC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 142
EcoO109I RGGNCCY 2 cut(s) 383, 711
EcoRII CCWGG 3 cut(s) 206, 483, 686
EcoT14I CCWWGG 2 cut(s) 675, 780
EcoT38I GRGCYC 3 cut(s) 144, 915, 972
ErhI CCWWGG 2 cut(s) 675, 780
FaeI CATG 4 cut(s) 95, 201, 316, 853
FatI CATG 4 cut(s) 91, 197, 312, 849
Fnu4HI GCNGC 3 cut(s) 112, 475, 534
FokI GGATG 2 cut(s) 41, 203
FriOI GRGCYC 3 cut(s) 144, 915, 972
Fsp4HI GCNGC 3 cut(s) 112, 475, 534
FspBI CTAG 1 cut(s) 377
GluI GCNGC 3 cut(s) 112, 475, 534
GsaI CCCAGC 1 cut(s) 969
HaeIII GGCC 2 cut(s) 713, 833
HapII CCGG 1 cut(s) 393
Hin1II CATG 4 cut(s) 95, 201, 316, 853
HindIII AAGCTT 1 cut(s) 622
HinfI GANTC 2 cut(s) 123, 203
HpaII CCGG 1 cut(s) 393
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 350, 552
Hpy188I TCNGA 3 cut(s) 362, 424, 463
Hpy188III TCNNGA 2 cut(s) 443, 838
Hpy8I GTNNAC 2 cut(s) 350, 552
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 15, 281
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 4 cut(s) 111, 620, 734, 895
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 2 cut(s) 303, 625
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 4 cut(s) 95, 201, 316, 853
KpnI GGTACC 1 cut(s) 399
Kzo9I GATC 5 cut(s) 21, 194, 419, 465, 718
LmnI GCTCC 2 cut(s) 139, 502
Lsp1109I GCAGC 3 cut(s) 123, 486, 520
MaeI CTAG 1 cut(s) 377
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 5 cut(s) 15, 124, 275, 281, 845
MalI GATC 5 cut(s) 23, 196, 421, 467, 720
MboI GATC 5 cut(s) 21, 194, 419, 465, 718
MboII GAAGA 7 cut(s) 25, 28, 31, 431, 551, 770, 890
MflI RGATCY 2 cut(s) 419, 465
MhlI GDGCHC 3 cut(s) 144, 915, 972
MlsI TGGCCA 1 cut(s) 833
MluNI TGGCCA 1 cut(s) 833
MlyI GAGTC 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 7 cut(s) 317, 363, 396, 694, 925, 962, 970
Mox20I TGGCCA 1 cut(s) 833
MscI TGGCCA 1 cut(s) 833
MseI TTAA 5 cut(s) 146, 255, 272, 452, 513
MslI CAYNNNNRTG 1 cut(s) 812
Msp20I TGGCCA 1 cut(s) 833
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 3 cut(s) 208, 485, 688
MvaI CCWGG 3 cut(s) 208, 485, 688
MwoI GCNNNNNNNGC 1 cut(s) 398
NdeII GATC 5 cut(s) 21, 194, 419, 465, 718
NlaIII CATG 4 cut(s) 95, 201, 316, 853
NlaIV GGNNCC 4 cut(s) 397, 421, 498, 712
NmuCI GTSAC 3 cut(s) 124, 275, 845
PfeI GAWTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 416
PfoI TCCNGGA 2 cut(s) 206, 483
PkrI GCNGC 3 cut(s) 113, 476, 535
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PpuMI RGGWCCY 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 383
Psp6I CCWGG 3 cut(s) 206, 483, 686
PspFI CCCAGC 1 cut(s) 965
PspGI CCWGG 3 cut(s) 206, 483, 686
PspN4I GGNNCC 4 cut(s) 397, 421, 498, 712
PspPI GGNCC 3 cut(s) 350, 383, 711
PspPPI RGGWCCY 1 cut(s) 383
PsuI RGATCY 2 cut(s) 419, 465
RsaI GTAC 4 cut(s) 397, 553, 752, 872
RsaNI GTAC 4 cut(s) 396, 552, 751, 871
RseI CAYNNNNRTG 1 cut(s) 812
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 5 cut(s) 146, 255, 272, 452, 513
SatI GCNGC 3 cut(s) 112, 475, 534
Sau3AI GATC 5 cut(s) 21, 194, 419, 465, 718
Sau96I GGNCC 3 cut(s) 350, 383, 711
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 3 cut(s) 208, 485, 688
SduI GDGCHC 3 cut(s) 144, 915, 972
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 2 cut(s) 350, 383
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 2 cut(s) 145, 165
SmoI CTYRAG 2 cut(s) 145, 165
SsiI CCGC 1 cut(s) 399
SspMI CTAG 1 cut(s) 377
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 3 cut(s) 206, 483, 686
StyI CCWWGG 2 cut(s) 675, 780
TaaI ACNGT 2 cut(s) 15, 281
TaiI ACGT 1 cut(s) 816
TaqII GACCGA 1 cut(s) 758
TatI WGTACW 2 cut(s) 551, 750
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 5 cut(s) 146, 255, 272, 452, 513
Tru9I TTAA 5 cut(s) 146, 255, 272, 452, 513
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 3 cut(s) 124, 275, 845
TseI GCWGC 3 cut(s) 111, 474, 533
Tsp45I GTSAC 3 cut(s) 124, 275, 845
TspDTI ATGAA 7 cut(s) 19, 70, 80, 591, 796, 866, 924
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 18
Van91I CCANNNNNTGG 1 cut(s) 416
Vha464I CTTAAG 1 cut(s) 145
VpaK11BI GGWCC 2 cut(s) 350, 383
XapI RAATTY 5 cut(s) 335, 364, 595, 631, 959
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.