RLG00000030081

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016547
Physical Location & Seq
Reverse (-)
54910562 .. 54914479
3918 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030081

Sequence Viewer

Length: 1026 bp
ATGGCAACCTTGCAGAAATTCAAGCTCCTCGCCACTCAATGCTCCGTCGTCGCCGGAAGCCCCACGCGCAGCCCATCCGCCAGCCCCGTCATCCACCTCCGCCGCCGCAAAACCCTCAGAATGTTCCTCTCCCGCCCCGACCGCCGCCGAATGCCTCGCCGGAGTACCTCCTCCGATGCTCCCAACAACGACGGTGACGGCGGCGATCGCGAAAATGACGACCCGCCGGAGAGGAAGAACAGCAAGGAGCAGCAGGCTCGGGTCCGCCGTAAGCTCAGGGACCTGTTCGTGTCGTCGCCGCCGCCGCTGGAGGATGGAAGGAGAGGCCGAGAGGTGGAAGATGGAGAGGAGCGAGGATTGCTGTCGGCGACGGACGTCGGTGGCGGCGGCGGCGGCGTCGGTGGCTTGGCGAGGACTCGGCGCGGCGGGTTGTTAAGTCGACCCCTGACGGCGTCGTTTAGGTGTAGATTGCTGAAGAGGGCTTGGCGACCTGTGCTGGTTCCTATTCCCGAATTGATATTGACCCAGGATATCACTACAAGTGATTTTGGATCATACATGGGTTTTGGTGTGGACTGTGGAGTAGTATGTAGAGGTGGTGGATACTGGCTAGCCGTGAGGGAAGGTGGAGTTTCTTTCTGCCCTGAGATTTTCATGGCCCGGCTGAAACTAAAAGGCTTCTTTTTTAGTCCTAATTTTCACAGAGAAGAAAAAAACACAGGGAGTCTGAAGAACGGGATTAGCTTGATGCCCCTCAAAGAAATGAAGGCAAAGTTAGAGGACGGTGGCGTCAGCCACAATTCTCCCAGCTACATGCTCAATTTTGAGGACGACACCACATGCGTCGACGACAACCTCCTCCGGTCGCAGCTGTCGGCCTTTGCCTCCTGGAGTTTGACCCCGCCGGAGAAGTCGACCACTAGCTGGCGTATTCTAAACGCCTTTCTAAAGGAAGATGAGGACAACCGGAGCTGGAGGTCACCGACGAGGAACAAAGCTGGAGGTCACCGAAGGCGAACAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

38.0

Weight (kDa)

10.08

Isoelectric Point (pI)

70.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016547)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66890 AT5G16200 AT5G16200
fragaria_vesca FvH4_7g03610
malus_domestica MD17G1247700.v1.1
prunus_persica Prupe.3G146400_v2.0.a1
pyrus_communis pycom09g17100 pycom17g24900
rosa_chinensis RchiOBHm_Chr1g0326241
rosa_laevigata RLG00000030081
rosa_multiflora Rmu_sc0004192.1_g000026
rosa_roxburghii Rroxscaffold_4G00323890
rosa_rugosa Rorug01G0058200
rosa_wichuraiana Rw1G006010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 788
AatII GACGTC 1 cut(s) 378
AccB7I CCANNNNNTGG 1 cut(s) 924
AccI GTMKAC 3 cut(s) 439, 846, 914
AccII CGCG 3 cut(s) 67, 210, 423
AclWI GGATC 1 cut(s) 559
AcsI RAATTY 1 cut(s) 17
AcuI CTGAAG 2 cut(s) 494, 749
AcyI GRCGYC 4 cut(s) 375, 396, 452, 789
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 2 cut(s) 334, 924
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 2 cut(s) 525, 887
AluBI AGCT 8 cut(s) 25, 274, 744, 810, 871, 924, 972, 998
AluI AGCT 8 cut(s) 25, 274, 744, 810, 871, 924, 972, 998
AlwI GGATC 1 cut(s) 559
Ama87I CYCGRG 1 cut(s) 258
AoxI GGCC 3 cut(s) 325, 657, 876
ApeKI GCWGC 3 cut(s) 69, 250, 868
ApoI RAATTY 1 cut(s) 17
AsiSI GCGATCGC 1 cut(s) 208
AspLEI GCGC 2 cut(s) 69, 423
AspS9I GGNCC 3 cut(s) 262, 280, 658
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 3 cut(s) 206, 972, 998
AsuNHI GCTAGC 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 258
AvaII GGWCC 2 cut(s) 262, 280
BbvI GCAGC 3 cut(s) 81, 262, 880
BccI CCATC 3 cut(s) 82, 308, 335
BceAI ACGGC 4 cut(s) 214, 252, 465, 599
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 2 cut(s) 527, 889
BciVI GTATCC 1 cut(s) 596
BcnI CCSGG 1 cut(s) 661
BfaI CTAG 2 cut(s) 611, 921
BfuI GTATCC 1 cut(s) 596
Bme1390I CCNGG 3 cut(s) 527, 661, 889
Bme18I GGWCC 2 cut(s) 262, 280
BmeT110I CYCGRG 1 cut(s) 258
BmgT120I GGNCC 3 cut(s) 262, 280, 658
BmiI GGNNCC 3 cut(s) 263, 281, 501
BmrFI CCNGG 3 cut(s) 527, 661, 889
BmsI GCATC 2 cut(s) 166, 738
BmtI GCTAGC 1 cut(s) 614
BoxI GACNNNNGTC 1 cut(s) 374
BpmI CTGGAG 4 cut(s) 329, 910, 994, 1020
Bpu10I CCTNAGC 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 661
BsaHI GRCGYC 4 cut(s) 375, 396, 452, 789
BsaJI CCNNGG 1 cut(s) 525
BsaWI WCCGGW 2 cut(s) 861, 966
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 924
Bse1I ACTGG 1 cut(s) 611
BseBI CCWGG 2 cut(s) 527, 889
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 3 cut(s) 74, 90, 319
BseLI CCNNNNNNNGG 2 cut(s) 334, 924
BseMII CTCAG 3 cut(s) 130, 289, 636
BseNI ACTGG 1 cut(s) 611
BseRI GAGGAG 4 cut(s) 17, 160, 362, 848
BseXI GCAGC 3 cut(s) 81, 262, 880
BseYI CCCAGC 1 cut(s) 806
Bsh1236I CGCG 3 cut(s) 67, 210, 423
Bsh1285I CGRYCG 3 cut(s) 142, 208, 866
BshFI GGCC 3 cut(s) 327, 659, 878
BsiEI CGRYCG 3 cut(s) 142, 208, 866
BsiHKCI CYCGRG 1 cut(s) 258
BsiSI CCGG 7 cut(s) 54, 160, 227, 661, 862, 905, 967
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 334, 924
BsmFI GGGAC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 3 cut(s) 327, 659, 878
BsoBI CYCGRG 1 cut(s) 258
Bsp143I GATC 2 cut(s) 205, 551
Bsp68I TCGCGA 1 cut(s) 210
BspANI GGCC 3 cut(s) 327, 659, 878
BspCNI CTCAG 3 cut(s) 129, 288, 637
BspFNI CGCG 3 cut(s) 67, 210, 423
BspLI GGNNCC 3 cut(s) 263, 281, 501
BspOI GCTAGC 1 cut(s) 614
BspPI GGATC 1 cut(s) 559
BsrI ACTGG 1 cut(s) 611
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 2 cut(s) 205, 551
BssNI GRCGYC 4 cut(s) 375, 396, 452, 789
Bst2UI CCWGG 2 cut(s) 527, 889
Bst4CI ACNGT 3 cut(s) 194, 578, 785
Bst6I CTCTTC 1 cut(s) 470
BstACI GRCGYC 4 cut(s) 375, 396, 452, 789
BstC8I GCNNGC 4 cut(s) 82, 255, 612, 926
BstDEI CTNAG 3 cut(s) 116, 275, 645
BstEII GGTNACC 2 cut(s) 978, 1004
BstF5I GGATG 3 cut(s) 74, 90, 319
BstFNI CGCG 3 cut(s) 67, 210, 423
BstHHI GCGC 2 cut(s) 69, 423
BstKTI GATC 2 cut(s) 208, 554
BstMBI GATC 2 cut(s) 205, 551
BstMCI CGRYCG 3 cut(s) 142, 208, 866
BstMWI GCNNNNNNNGC 9 cut(s) 66, 141, 207, 304, 358, 390, 393, 402, 493
BstNI CCWGG 2 cut(s) 527, 889
BstNSI RCATGY 2 cut(s) 817, 843
BstPAI GACNNNNGTC 1 cut(s) 374
BstPI GGTNACC 2 cut(s) 978, 1004
BstSCI CCNGG 3 cut(s) 525, 659, 887
BstUI CGCG 3 cut(s) 67, 210, 423
BstV1I GCAGC 3 cut(s) 81, 262, 880
BsuI GTATCC 1 cut(s) 596
BsuRI GGCC 3 cut(s) 327, 659, 878
BtsCI GGATG 3 cut(s) 74, 90, 319
BtuMI TCGCGA 1 cut(s) 210
Cac8I GCNNGC 4 cut(s) 82, 255, 612, 926
CfoI GCGC 2 cut(s) 69, 423
Cfr13I GGNCC 3 cut(s) 262, 280, 658
CseI GACGC 4 cut(s) 385, 441, 778, 832
Csp6I GTAC 1 cut(s) 165
CviAII CATG 4 cut(s) 559, 655, 814, 840
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 3 cut(s) 116, 275, 645
DpnI GATC 2 cut(s) 207, 553
DpnII GATC 2 cut(s) 205, 551
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
EciI GGCGGA 3 cut(s) 67, 89, 254
Eco32I GATATC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 262, 280
Eco57I CTGAAG 2 cut(s) 494, 749
Eco88I CYCGRG 1 cut(s) 258
Eco91I GGTNACC 2 cut(s) 978, 1004
EcoO109I RGGNCCY 1 cut(s) 280
EcoO65I GGTNACC 2 cut(s) 978, 1004
EcoRII CCWGG 2 cut(s) 525, 887
EcoRV GATATC 1 cut(s) 532
FaeI CATG 4 cut(s) 562, 658, 817, 843
FaiI YATR 6 cut(s) 556, 560, 589, 656, 815, 841
FaqI GGGAC 1 cut(s) 293
FatI CATG 4 cut(s) 558, 654, 813, 839
FauI CCCGC 4 cut(s) 140, 231, 419, 909
FblI GTMKAC 3 cut(s) 439, 846, 914
FokI GGATG 3 cut(s) 61, 77, 326
FspBI CTAG 2 cut(s) 611, 921
GlaI GCGC 2 cut(s) 68, 422
GsaI CCCAGC 1 cut(s) 810
GsuI CTGGAG 4 cut(s) 329, 910, 994, 1020
HaeIII GGCC 3 cut(s) 327, 659, 878
HapII CCGG 7 cut(s) 54, 160, 227, 661, 862, 905, 967
HgaI GACGC 4 cut(s) 385, 441, 778, 832
HhaI GCGC 2 cut(s) 69, 423
Hin1I GRCGYC 4 cut(s) 375, 396, 452, 789
Hin1II CATG 4 cut(s) 562, 658, 817, 843
Hin6I GCGC 2 cut(s) 67, 421
HinP1I GCGC 2 cut(s) 67, 421
HincII GTYRAC 3 cut(s) 440, 847, 915
HindII GTYRAC 3 cut(s) 440, 847, 915
HinfI GANTC 2 cut(s) 415, 724
HpaII CCGG 7 cut(s) 54, 160, 227, 661, 862, 905, 967
HphI GGTGA 3 cut(s) 206, 972, 998
Hpy166II GTNNAC 4 cut(s) 440, 574, 847, 915
Hpy188I TCNGA 4 cut(s) 119, 175, 729, 1025
Hpy188III TCNNGA 2 cut(s) 209, 509
Hpy8I GTNNAC 4 cut(s) 440, 574, 847, 915
HpyAV CCTTC 4 cut(s) 312, 617, 760, 1005
HpyCH4III ACNGT 3 cut(s) 194, 578, 785
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 9 cut(s) 66, 141, 207, 304, 358, 390, 393, 402, 493
HpyF3I CTNAG 3 cut(s) 116, 275, 645
HpySE526I ACGT 1 cut(s) 375
Hsp92I GRCGYC 4 cut(s) 375, 396, 452, 789
Hsp92II CATG 4 cut(s) 562, 658, 817, 843
HspAI GCGC 2 cut(s) 67, 421
Kzo9I GATC 2 cut(s) 205, 551
LmnI GCTCC 6 cut(s) 30, 47, 184, 247, 349, 969
Lsp1109I GCAGC 3 cut(s) 81, 262, 880
LweI GCATC 2 cut(s) 166, 738
MaeI CTAG 2 cut(s) 611, 921
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 3 cut(s) 194, 978, 1004
MalI GATC 2 cut(s) 207, 553
MboI GATC 2 cut(s) 205, 551
MboII GAAGA 6 cut(s) 247, 350, 487, 719, 742, 965
MluCI AATT 5 cut(s) 17, 512, 694, 799, 820
MlyI GAGTC 2 cut(s) 409, 733
MseI TTAA 1 cut(s) 434
MspA1I CMGCKG 2 cut(s) 307, 871
MspI CCGG 7 cut(s) 54, 160, 227, 661, 862, 905, 967
MspR9I CCNGG 3 cut(s) 527, 661, 889
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 2 cut(s) 527, 889
MvnI CGCG 3 cut(s) 67, 210, 423
MwoI GCNNNNNNNGC 9 cut(s) 66, 141, 207, 304, 358, 390, 393, 402, 493
NciI CCSGG 1 cut(s) 661
NdeII GATC 2 cut(s) 205, 551
NheI GCTAGC 1 cut(s) 610
NlaIII CATG 4 cut(s) 562, 658, 817, 843
NlaIV GGNNCC 3 cut(s) 263, 281, 501
NmeAIII GCCGAG 2 cut(s) 353, 397
NmuCI GTSAC 3 cut(s) 194, 978, 1004
NruI TCGCGA 1 cut(s) 210
NspI RCATGY 2 cut(s) 817, 843
PcsI WCGNNNNNNNCGW 1 cut(s) 265
PctI GAATGC 1 cut(s) 156
PflFI GACNNNGTC 1 cut(s) 451
PflMI CCANNNNNTGG 1 cut(s) 924
PfoI TCCNGGA 1 cut(s) 887
Ple19I CGATCG 1 cut(s) 208
PleI GAGTC 2 cut(s) 409, 732
PpsI GAGTC 2 cut(s) 409, 732
PpuMI RGGWCCY 1 cut(s) 280
PshAI GACNNNNGTC 1 cut(s) 374
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 525, 887
PspEI GGTNACC 2 cut(s) 978, 1004
PspFI CCCAGC 1 cut(s) 806
PspGI CCWGG 2 cut(s) 525, 887
PspN4I GGNNCC 3 cut(s) 263, 281, 501
PspPI GGNCC 3 cut(s) 262, 280, 658
PspPPI RGGWCCY 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 451
PvuI CGATCG 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 871
RgaI GCGATCGC 1 cut(s) 208
RruI TCGCGA 1 cut(s) 210
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
SalI GTCGAC 3 cut(s) 438, 845, 913
SaqAI TTAA 1 cut(s) 434
Sau3AI GATC 2 cut(s) 205, 551
Sau96I GGNCC 3 cut(s) 262, 280, 658
SchI GAGTC 2 cut(s) 409, 733
ScrFI CCNGG 3 cut(s) 527, 661, 889
SfaAI GCGATCGC 1 cut(s) 208
SfaNI GCATC 2 cut(s) 166, 738
SgfI GCGATCGC 1 cut(s) 208
SgrDI CGTCGACG 1 cut(s) 845
SinI GGWCC 2 cut(s) 262, 280
Sse9I AATT 5 cut(s) 17, 512, 694, 799, 820
SspMI CTAG 2 cut(s) 611, 921
StyD4I CCNGG 3 cut(s) 525, 659, 887
TaaI ACNGT 3 cut(s) 194, 578, 785
TaiI ACGT 1 cut(s) 378
TaqI TCGA 3 cut(s) 439, 846, 914
TasI AATT 5 cut(s) 17, 512, 694, 799, 820
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TseFI GTSAC 3 cut(s) 194, 978, 1004
TseI GCWGC 3 cut(s) 69, 250, 868
Tsp45I GTSAC 3 cut(s) 194, 978, 1004
TspDTI ATGAA 2 cut(s) 643, 779
TspGWI ACGGA 2 cut(s) 34, 386
Tth111I GACNNNGTC 1 cut(s) 451
Van91I CCANNNNNTGG 1 cut(s) 924
VpaK11BI GGWCC 2 cut(s) 262, 280
XapI RAATTY 1 cut(s) 17
XceI RCATGY 2 cut(s) 817, 843
XmiI GTMKAC 3 cut(s) 439, 846, 914
XspI CTAG 2 cut(s) 611, 921
ZraI GACGTC 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.