RLG00000033433

protein phosphatase 2C 39

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011687
Physical Location & Seq
Forward (+)
27103700 .. 27109964
6265 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033433

Sequence Viewer

Length: 1467 bp
ATGACAGGCAAGGAAATCCTCAACAAGATGAAGGAAAAAGTTTTAGGTCCATCGGACCCTGACTCTGGCAAAGGTAAGAGCAAGATGTCACATAATATATCACATGGTTACCACTTGGTCAAGGGAAAATCCCATCATGCTATGGAAGACTATGTTGTTGCACAGTTTAAGCAAATTGACGACCATGAGCTAGGACTATTTGCAATCTTTGATGGGCATTTGAGTCATGAAATTCCTGAATACTTGCAGTCTAATCTCTTCAATAATATCTTAAAAGAGCCCGAATTCTGGAAAGAAACTGAGGATGCAGTCAGGAAAGCATATCGAATAACTGATACTAACATTCTGGAGAAAGCAGTCGATTTGGGCAAAGGGGGCTCAACTGCTGTTACTGCCATATTAATTGACTGTCGCAAGCTTGTGGTAGCGAATGTTGGGGATTCTCGTGCTATTATCTGCAAGAATGGTGTGGCTAAGCAGCTATCTGTTGATCATGAACCGAGCACAGAGAGAGAGCAAATCGAGAACAGAGGTGGTTTTGTATCAAACTTTCCAGGGGATGTACCACGTGTTGATGGGCAGTTGGCTGTAGCACGGGCATTCGGTGACAAGAGCTTGAAGAAACATTTAAGTTCTGAACCTTTTGTGATGTCGGAGACGATAGATGATGAAACAGAGTTTGCTGTCTTAGCAAGTGATGGACTGTGGAAGGTGATGACAAACCAAGAAGTTGTGGATGCCATAAAAAATATAAAGGATGCTCGGGCAGCAGCAAAGCATCTTACTGAAGAGGCAGTCAATAGGAAGAGCTCAGACGATATTTCCTGTGTAGTTCTGCCGCCGCCTCCGTCTCGAAGGACGCATGCCACCGTCGAGAGGCAGGTAGCTGAGGTTGTGGTGAGGCCGAGGACGAGGTTGGCTGTGAAAAAGCTGAAGGAGGAGGAGGTGCTGCCAGAGCCGGAGAAGGAGAAGGAGAAGGACCGCGTGATTGTGATTTCGGAGAAGGAAGACTCCAATTCGGAGGGTGAAAAGGAGGAAGAAGAAGAAGAGAACAAAGCTGTGATGGCTGACGATAGTGGTGGCTTGAGTGCTAACAAGGCTGCTGGGCAAGAAGAAGAGGGTAGTACGGCGCCGTTTCTTGAAAAGACCCGCGACCGACTTCCGCTATGCACTAGAGCTGCACGACTCCGAGTTTGTACTTGGCCCACCACTATCAGAATCGCCGGAATGAGAAGTGGAGAAGGCCGCGTCAGAGAGACCCTGATCGAGTTCGTTGGAGGGGGGCTGGGGGCATTCCAGAGAGAGGAGAGAGAATCTGCTGGAGACATGCGTTTTGTTGGCGTTGACGACTTTGAAGGTGGCAAGAGGGAAGCGGGAGGTGAGGTTGAGGGAGGGCATCGTCGTCGTCTTCAGGCGTGGACACCATTGCTGCGCTGGAGGTGGAGATCGAAGATCGGAGATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

489

Amino Acids

54.22

Weight (kDa)

5.78

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 45 - 274 2.5e-54 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011687)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15260 AT3G15260
fragaria_vesca FvH4_3g19640 FvH4_3g19640
malus_domestica MD03G1230700.v1.1 MD11G1251900.v1.1
prunus_persica Prupe.4G177100_v2.0.a1
pyrus_communis pycom03g17840 pycom11g22340
rosa_chinensis RchiOBHm_Chr5g0032841
rosa_laevigata RLG00000033433
rosa_multiflora Rmu_sc0000986.1_g000035
rosa_roxburghii Rroxscaffold_1G00047190
rosa_rugosa Rorug05G0134100
rosa_samantha Rh5AG227000 Rh5BG228300 Rh5CG256100 Rh5DG233100
rosa_wichuraiana Rw5G020810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 871
AccB1I GGYRCC 1 cut(s) 1129
AccII CGCG 3 cut(s) 984, 1152, 1248
AciI CCGC 7 cut(s) 839, 842, 982, 1150, 1163, 1246, 1373
AcsI RAATTY 2 cut(s) 231, 284
AcuI CTGAAG 3 cut(s) 807, 953, 1394
AcvI CACGTG 1 cut(s) 569
AcyI GRCGYC 1 cut(s) 1130
AfaI GTAC 3 cut(s) 564, 1126, 1198
AfiI CCNNNNNNNGG 4 cut(s) 65, 288, 876, 1303
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 4 cut(s) 262, 619, 1142, 1355
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 9 cut(s) 190, 418, 481, 615, 810, 887, 931, 1058, 1178
AluI AGCT 9 cut(s) 190, 418, 481, 615, 810, 887, 931, 1058, 1178
Alw21I GWGCWC 2 cut(s) 506, 812
Alw26I GTCTC 4 cut(s) 650, 855, 1250, 1317
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 3 cut(s) 902, 1202, 1243
ApeKI GCWGC 7 cut(s) 478, 767, 770, 949, 1100, 1178, 1429
ApoI RAATTY 2 cut(s) 231, 284
ArsI GACNNNNNNTTYG 4 cut(s) 1176, 1208, 1316, 1348
AseI ATTAAT 1 cut(s) 401
AspLEI GCGC 2 cut(s) 1132, 1434
AspS9I GGNCC 4 cut(s) 47, 55, 979, 1203
AsuHPI GGTGA 5 cut(s) 617, 724, 910, 1037, 1391
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 3 cut(s) 47, 55, 979
BanI GGYRCC 1 cut(s) 1129
BanII GRGCYC 3 cut(s) 282, 380, 812
BauI CACGAG 1 cut(s) 444
BbrPI CACGTG 1 cut(s) 569
BbsI GAAGAC 3 cut(s) 153, 1014, 1400
Bbv12I GWGCWC 2 cut(s) 506, 812
BbvCI CCTCAGC 1 cut(s) 888
BbvI GCAGC 7 cut(s) 490, 779, 782, 936, 1087, 1165, 1416
BccI CCATC 7 cut(s) 58, 141, 206, 569, 692, 1057, 1454
BceAI ACGGC 2 cut(s) 1117, 1143
BcgI CGANNNNNNTGC 2 cut(s) 1385, 1419
BciT130I CCWGG 1 cut(s) 555
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 4 cut(s) 650, 855, 1250, 1317
BfaI CTAG 2 cut(s) 191, 1173
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 1133
BfuAI ACCTGC 1 cut(s) 871
BlpI GCTNAGC 1 cut(s) 474
Bme1390I CCNGG 1 cut(s) 555
Bme18I GGWCC 3 cut(s) 47, 55, 979
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 4 cut(s) 47, 55, 979, 1203
BmiI GGNNCC 2 cut(s) 57, 1131
BmrFI CCNGG 1 cut(s) 555
BmsI GCATC 5 cut(s) 295, 727, 748, 787, 1405
BpiI GAAGAC 3 cut(s) 153, 1014, 1400
BpmI CTGGAG 3 cut(s) 368, 1341, 1456
Bpu10I CCTNAGC 1 cut(s) 888
Bpu1102I GCTNAGC 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 1105
BsaAI YACGTR 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 1130
BsaI GGTCTC 1 cut(s) 1250
BsaJI CCNNGG 2 cut(s) 554, 905
BsaXI ACNNNNNCTCC 2 cut(s) 1368, 1398
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 288, 876, 1303
Bse3DI GCAATG 1 cut(s) 1424
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 554, 905
BseGI GGATG 4 cut(s) 310, 565, 742, 763
BseLI CCNNNNNNNGG 4 cut(s) 65, 288, 876, 1303
BseMI GCAATG 1 cut(s) 1424
BseMII CTCAG 3 cut(s) 291, 825, 879
BseRI GAGGAG 3 cut(s) 953, 956, 1319
BseXI GCAGC 7 cut(s) 490, 779, 782, 936, 1087, 1165, 1416
BseYI CCCAGC 2 cut(s) 1103, 1285
BsgI GTGCAG 1 cut(s) 1164
Bsh1236I CGCG 3 cut(s) 984, 1152, 1248
Bsh1285I CGRYCG 1 cut(s) 1156
BshFI GGCC 3 cut(s) 904, 1204, 1245
BshNI GGYRCC 1 cut(s) 1129
BsiEI CGRYCG 1 cut(s) 1156
BsiHKAI GWGCWC 2 cut(s) 506, 812
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 2 cut(s) 959, 1224
BslI CCNNNNNNNGG 4 cut(s) 65, 288, 876, 1303
BsmAI GTCTC 4 cut(s) 650, 855, 1250, 1317
BsmBI CGTCTC 2 cut(s) 650, 855
BsmI GAATGC 2 cut(s) 599, 1292
BsnI GGCC 3 cut(s) 904, 1204, 1245
Bso31I GGTCTC 1 cut(s) 1250
BsoBI CYCGRG 1 cut(s) 762
Bsp1286I GDGCHC 4 cut(s) 282, 380, 506, 812
Bsp143I GATC 4 cut(s) 490, 1263, 1445, 1452
Bsp1720I GCTNAGC 1 cut(s) 474
BspACI CCGC 7 cut(s) 839, 842, 982, 1150, 1163, 1246, 1373
BspANI GGCC 3 cut(s) 904, 1204, 1245
BspCNI CTCAG 3 cut(s) 292, 824, 880
BspFNI CGCG 3 cut(s) 984, 1152, 1248
BspHI TCATGA 2 cut(s) 226, 493
BspLI GGNNCC 2 cut(s) 57, 1131
BspMI ACCTGC 1 cut(s) 871
BspQI GCTCTTC 1 cut(s) 800
BspT107I GGYRCC 1 cut(s) 1129
BspTNI GGTCTC 1 cut(s) 1250
BsrDI GCAATG 1 cut(s) 1424
BssECI CCNNGG 2 cut(s) 554, 905
BssMI GATC 4 cut(s) 490, 1263, 1445, 1452
BssNI GRCGYC 1 cut(s) 1130
BssSI CACGAG 1 cut(s) 444
Bst2BI CACGAG 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 4 cut(s) 165, 410, 705, 871
Bst6I CTCTTC 5 cut(s) 263, 783, 800, 1041, 1110
BstACI GRCGYC 1 cut(s) 1130
BstBAI YACGTR 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 416, 864
BstDEI CTNAG 5 cut(s) 300, 474, 688, 811, 888
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 4 cut(s) 310, 565, 742, 763
BstFNI CGCG 3 cut(s) 984, 1152, 1248
BstH2I RGCGCY 1 cut(s) 1133
BstHHI GCGC 2 cut(s) 1132, 1434
BstKTI GATC 4 cut(s) 493, 1266, 1448, 1455
BstMAI GTCTC 4 cut(s) 650, 855, 1250, 1317
BstMBI GATC 4 cut(s) 490, 1263, 1445, 1452
BstMCI CGRYCG 1 cut(s) 1156
BstMWI GCNNNNNNNGC 7 cut(s) 375, 392, 689, 767, 955, 1064, 1097
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 2 cut(s) 866, 1330
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 553
BstSFI CTRYAG 1 cut(s) 588
BstUI CGCG 3 cut(s) 984, 1152, 1248
BstV1I GCAGC 7 cut(s) 490, 779, 782, 936, 1087, 1165, 1416
BstV2I GAAGAC 3 cut(s) 153, 1014, 1400
BsuRI GGCC 3 cut(s) 904, 1204, 1245
BtsCI GGATG 4 cut(s) 310, 565, 742, 763
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 2 cut(s) 416, 864
CciI TCATGA 2 cut(s) 226, 493
CfoI GCGC 2 cut(s) 1132, 1434
Cfr13I GGNCC 4 cut(s) 47, 55, 979, 1203
CseI GACGC 2 cut(s) 868, 1237
Csp6I GTAC 3 cut(s) 563, 1125, 1197
CviAII CATG 7 cut(s) 104, 137, 185, 227, 494, 863, 1327
CviQI GTAC 3 cut(s) 563, 1125, 1197
DdeI CTNAG 5 cut(s) 300, 474, 688, 811, 888
DinI GGCGCC 1 cut(s) 1131
DpnI GATC 4 cut(s) 492, 1265, 1447, 1454
DpnII GATC 4 cut(s) 490, 1263, 1445, 1452
Eam1104I CTCTTC 5 cut(s) 263, 783, 800, 1041, 1110
EarI CTCTTC 5 cut(s) 263, 783, 800, 1041, 1110
Ecl136II GAGCTC 1 cut(s) 810
Eco24I GRGCYC 3 cut(s) 282, 380, 812
Eco31I GGTCTC 1 cut(s) 1250
Eco47I GGWCC 3 cut(s) 47, 55, 979
Eco53kI GAGCTC 1 cut(s) 810
Eco57I CTGAAG 3 cut(s) 807, 953, 1394
Eco72I CACGTG 1 cut(s) 569
Eco88I CYCGRG 1 cut(s) 762
Eco91I GGTNACC 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 810
EcoO65I GGTNACC 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 553
EcoT38I GRGCYC 3 cut(s) 282, 380, 812
EgeI GGCGCC 1 cut(s) 1131
EheI GGCGCC 1 cut(s) 1131
Esp3I CGTCTC 2 cut(s) 650, 855
FaeI CATG 7 cut(s) 107, 140, 188, 230, 497, 866, 1330
FatI CATG 7 cut(s) 103, 136, 184, 226, 493, 862, 1326
FauI CCCGC 2 cut(s) 1157, 1366
FbaI TGATCA 1 cut(s) 490
FokI GGATG 4 cut(s) 317, 572, 749, 770
FriOI GRGCYC 3 cut(s) 282, 380, 812
FspBI CTAG 2 cut(s) 191, 1173
GlaI GCGC 2 cut(s) 1131, 1433
GsaI CCCAGC 2 cut(s) 1107, 1289
GsuI CTGGAG 3 cut(s) 368, 1341, 1456
HaeII RGCGCY 1 cut(s) 1133
HaeIII GGCC 3 cut(s) 904, 1204, 1245
HapII CCGG 2 cut(s) 959, 1224
HgaI GACGC 2 cut(s) 868, 1237
HhaI GCGC 2 cut(s) 1132, 1434
Hin1I GRCGYC 1 cut(s) 1130
Hin1II CATG 7 cut(s) 107, 140, 188, 230, 497, 866, 1330
Hin6I GCGC 2 cut(s) 1130, 1432
HinP1I GCGC 2 cut(s) 1130, 1432
HincII GTYRAC 1 cut(s) 1345
HindII GTYRAC 1 cut(s) 1345
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 7 cut(s) 62, 223, 440, 1010, 1185, 1218, 1313
HpaII CCGG 2 cut(s) 959, 1224
HphI GGTGA 5 cut(s) 617, 724, 910, 1037, 1391
Hpy166II GTNNAC 2 cut(s) 1345, 1419
Hpy8I GTNNAC 2 cut(s) 1345, 1419
Hpy99I CGWCG 3 cut(s) 875, 1404, 1407
HpyCH4III ACNGT 4 cut(s) 165, 410, 705, 871
HpyCH4IV ACGT 1 cut(s) 568
HpyCH4V TGCA 7 cut(s) 161, 203, 247, 308, 459, 1170, 1181
HpyF10VI GCNNNNNNNGC 7 cut(s) 375, 392, 689, 767, 955, 1064, 1097
HpyF3I CTNAG 5 cut(s) 300, 474, 688, 811, 888
HpySE526I ACGT 1 cut(s) 568
Hsp92I GRCGYC 1 cut(s) 1130
Hsp92II CATG 7 cut(s) 107, 140, 188, 230, 497, 866, 1330
HspAI GCGC 2 cut(s) 1130, 1432
KasI GGCGCC 1 cut(s) 1129
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 4 cut(s) 490, 1263, 1445, 1452
LguI GCTCTTC 1 cut(s) 800
Lsp1109I GCAGC 7 cut(s) 490, 779, 782, 936, 1087, 1165, 1416
LweI GCATC 5 cut(s) 295, 727, 748, 787, 1405
MaeI CTAG 2 cut(s) 191, 1173
MaeII ACGT 1 cut(s) 568
MaeIII GTNAC 4 cut(s) 87, 107, 388, 605
MalI GATC 4 cut(s) 492, 1265, 1447, 1454
MboI GATC 4 cut(s) 490, 1263, 1445, 1452
MhlI GDGCHC 4 cut(s) 282, 380, 506, 812
MluCI AATT 5 cut(s) 174, 231, 284, 402, 1015
Mly113I GGCGCC 1 cut(s) 1130
MlyI GAGTC 4 cut(s) 56, 232, 1004, 1179
MmeI TCCRAC 2 cut(s) 633, 1255
MseI TTAA 4 cut(s) 168, 272, 401, 629
MspI CCGG 2 cut(s) 959, 1224
MspR9I CCNGG 1 cut(s) 555
Mva1269I GAATGC 2 cut(s) 599, 1292
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 3 cut(s) 984, 1152, 1248
MwoI GCNNNNNNNGC 7 cut(s) 375, 392, 689, 767, 955, 1064, 1097
NarI GGCGCC 1 cut(s) 1130
NdeII GATC 4 cut(s) 490, 1263, 1445, 1452
NlaIII CATG 7 cut(s) 107, 140, 188, 230, 497, 866, 1330
NlaIV GGNNCC 2 cut(s) 57, 1131
NmeAIII GCCGAG 1 cut(s) 930
NmuCI GTSAC 2 cut(s) 87, 605
NspI RCATGY 2 cut(s) 866, 1330
PaeI GCATGC 1 cut(s) 866
PagI TCATGA 2 cut(s) 226, 493
PciSI GCTCTTC 1 cut(s) 800
PctI GAATGC 2 cut(s) 599, 1292
PfeI GAWTC 3 cut(s) 440, 1218, 1313
PleI GAGTC 4 cut(s) 56, 231, 1004, 1179
PluTI GGCGCC 1 cut(s) 1133
PmaCI CACGTG 1 cut(s) 569
PmlI CACGTG 1 cut(s) 569
PpsI GAGTC 4 cut(s) 56, 231, 1004, 1179
Ppu21I YACGTR 1 cut(s) 569
PshBI ATTAAT 1 cut(s) 401
Psp124BI GAGCTC 1 cut(s) 812
Psp6I CCWGG 1 cut(s) 553
PspCI CACGTG 1 cut(s) 569
PspEI GGTNACC 1 cut(s) 107
PspFI CCCAGC 2 cut(s) 1103, 1285
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 2 cut(s) 57, 1131
PspPI GGNCC 4 cut(s) 47, 55, 979, 1203
RsaI GTAC 3 cut(s) 564, 1126, 1198
RsaNI GTAC 3 cut(s) 563, 1125, 1197
SacI GAGCTC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 800
SaqAI TTAA 4 cut(s) 168, 272, 401, 629
Sau3AI GATC 4 cut(s) 490, 1263, 1445, 1452
Sau96I GGNCC 4 cut(s) 47, 55, 979, 1203
SchI GAGTC 4 cut(s) 56, 232, 1004, 1179
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 4 cut(s) 282, 380, 506, 812
SfaNI GCATC 5 cut(s) 295, 727, 748, 787, 1405
SfcI CTRYAG 1 cut(s) 588
SfoI GGCGCC 1 cut(s) 1131
SinI GGWCC 3 cut(s) 47, 55, 979
SmlI CTYRAG 1 cut(s) 1084
SmoI CTYRAG 1 cut(s) 1084
SphI GCATGC 1 cut(s) 866
Sse9I AATT 5 cut(s) 174, 231, 284, 402, 1015
SsiI CCGC 7 cut(s) 839, 842, 982, 1150, 1163, 1246, 1373
SspDI GGCGCC 1 cut(s) 1129
SspMI CTAG 2 cut(s) 191, 1173
SstI GAGCTC 1 cut(s) 812
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 4 cut(s) 165, 410, 705, 871
TaiI ACGT 1 cut(s) 571
TaqI TCGA 7 cut(s) 325, 360, 522, 853, 873, 1266, 1448
TaqII GACCGA 1 cut(s) 1170
TasI AATT 5 cut(s) 174, 231, 284, 402, 1015
TatI WGTACW 1 cut(s) 1196
TauI GCSGC 3 cut(s) 841, 844, 1248
TfiI GAWTC 3 cut(s) 440, 1218, 1313
Tru1I TTAA 4 cut(s) 168, 272, 401, 629
Tru9I TTAA 4 cut(s) 168, 272, 401, 629
TseFI GTSAC 2 cut(s) 87, 605
TseI GCWGC 7 cut(s) 478, 767, 770, 949, 1100, 1178, 1429
Tsp45I GTSAC 2 cut(s) 87, 605
TspDTI ATGAA 4 cut(s) 44, 243, 510, 684
TspGWI ACGGA 1 cut(s) 837
VpaK11BI GGWCC 3 cut(s) 47, 55, 979
VspI ATTAAT 1 cut(s) 401
XapI RAATTY 2 cut(s) 231, 284
XceI RCATGY 2 cut(s) 866, 1330
XcmI CCANNNNNNNNNTGG 1 cut(s) 1431
XspI CTAG 2 cut(s) 191, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.