Rmu_co8219862.1_g000001

Protein kinase PVPK-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8219862.1
OG0017638
Physical Location & Seq
Forward (+)
157 .. 723
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8219862.1_g000001.1.cds

Sequence Viewer

Length: 567 bp
atgtatagaggtagcactggaagtgacgttagtgatgagagcagctccagtagtttgagcaataacatgtacaagccccacaaggcgaatgatgtgagatgggaagcaattcaagctgtccgagatcatgatgggatgctgggtttgaatcatttcaagttgttgaagagactgggatgtggagatataggcagtgtttatctgtctgaactgtcgggcactagaacttgttttgccatgaaggttatggataaagcagcactggccagtcgcaaaaagcttttgagggctcagacagaaagagagatactacagtctctggatcaccctttcctgcccacattgtattcgcactttgagacagagaagttcacatgcttgttgatggagtattgccctggaggtgatctgcatgcactcaggcaaaaacaacctggaaagtattttccagagcatgctgccaggttagatattcctttccacttctttcagtttattataatgactacatgtattgcaccaaattaccttatttcgtcaatcacatggatatttacaaatgactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

188

Amino Acids

21.39

Weight (kDa)

7.7

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AclWI GGATC 1 cut(s) 330
AcoI YGGCCR 1 cut(s) 264
AfaI GTAC 1 cut(s) 71
AflIII ACRYGT 2 cut(s) 66, 509
AgsI TTSAA 4 cut(s) 113, 148, 157, 166
AjnI CCWGG 3 cut(s) 397, 433, 461
AluBI AGCT 3 cut(s) 45, 116, 280
AluI AGCT 3 cut(s) 45, 116, 280
Alw26I GTCTC 3 cut(s) 163, 321, 353
AlwI GGATC 1 cut(s) 330
AlwNI CAGNNNCTG 1 cut(s) 319
AoxI GGCC 1 cut(s) 264
ApeKI GCWGC 3 cut(s) 42, 257, 458
Asp700I GAANNNNTTC 2 cut(s) 108, 152
AsuHPI GGTGA 2 cut(s) 317, 416
BaeGI GKGCMC 1 cut(s) 221
BaeI ACNNNNGTAYC 2 cut(s) 299, 332
BalI TGGCCA 1 cut(s) 266
BanII GRGCYC 1 cut(s) 292
BbvI GCAGC 3 cut(s) 54, 269, 445
BccI CCATC 3 cut(s) 93, 125, 379
BciT130I CCWGG 3 cut(s) 399, 435, 463
BcoDI GTCTC 3 cut(s) 163, 321, 353
BfaI CTAG 2 cut(s) 222, 565
BfmI CTRYAG 1 cut(s) 311
BisI GCNGC 3 cut(s) 43, 258, 459
BlsI GCNGC 3 cut(s) 44, 259, 460
Bme1390I CCNGG 3 cut(s) 399, 435, 463
BmrFI CCNGG 3 cut(s) 399, 435, 463
BmrI ACTGGG 1 cut(s) 182
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 182
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 2 cut(s) 31, 420
BsaJI CCNNGG 1 cut(s) 397
Bse1I ACTGG 5 cut(s) 22, 48, 177, 267, 267
BseBI CCWGG 3 cut(s) 399, 435, 463
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 2 cut(s) 141, 182
BseMII CTCAG 2 cut(s) 305, 433
BseNI ACTGG 5 cut(s) 22, 48, 177, 267, 267
BseSI GKGCMC 1 cut(s) 221
BseXI GCAGC 3 cut(s) 54, 269, 445
BseYI CCCAGC 1 cut(s) 139
BshFI GGCC 1 cut(s) 266
BsmAI GTCTC 3 cut(s) 163, 321, 353
BsnI GGCC 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 221, 292
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 3 cut(s) 124, 322, 406
BspANI GGCC 1 cut(s) 266
BspCNI CTCAG 2 cut(s) 304, 432
BspHI TCATGA 1 cut(s) 127
BspPI GGATC 1 cut(s) 330
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 5 cut(s) 22, 48, 177, 267, 267
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 3 cut(s) 124, 322, 406
Bst2UI CCWGG 3 cut(s) 399, 435, 463
Bst4CI ACNGT 2 cut(s) 213, 315
Bst6I CTCTTC 1 cut(s) 161
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 414, 456
BstDEI CTNAG 2 cut(s) 291, 419
BstF5I GGATG 2 cut(s) 141, 182
BstKTI GATC 3 cut(s) 127, 325, 409
BstMAI GTCTC 3 cut(s) 163, 321, 353
BstMBI GATC 3 cut(s) 124, 322, 406
BstMWI GCNNNNNNNGC 2 cut(s) 113, 263
BstNI CCWGG 3 cut(s) 399, 435, 463
BstNSI RCATGY 5 cut(s) 70, 378, 416, 458, 513
BstSCI CCNGG 3 cut(s) 397, 433, 461
BstSFI CTRYAG 1 cut(s) 311
BstSLI GKGCMC 1 cut(s) 221
BstV1I GCAGC 3 cut(s) 54, 269, 445
BsuRI GGCC 1 cut(s) 266
BtsCI GGATG 2 cut(s) 141, 182
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 3 cut(s) 15, 199, 260
Cac8I GCNNGC 2 cut(s) 414, 456
CaiI CAGNNNCTG 1 cut(s) 319
CciI TCATGA 1 cut(s) 127
Csp6I GTAC 1 cut(s) 70
CviAII CATG 8 cut(s) 67, 128, 238, 375, 413, 455, 510, 546
CviJI RGCY 6 cut(s) 45, 76, 116, 266, 280, 290
CviKI_1 RGCY 6 cut(s) 45, 76, 116, 266, 280, 290
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 2 cut(s) 291, 419
DpnI GATC 3 cut(s) 126, 324, 408
DpnII GATC 3 cut(s) 124, 322, 406
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 292
EcoRII CCWGG 3 cut(s) 397, 433, 461
EcoT38I GRGCYC 1 cut(s) 292
FaeI CATG 8 cut(s) 70, 131, 241, 378, 416, 458, 513, 549
FatI CATG 8 cut(s) 66, 127, 237, 374, 412, 454, 509, 545
Fnu4HI GCNGC 3 cut(s) 43, 258, 459
FokI GGATG 2 cut(s) 148, 189
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 3 cut(s) 43, 258, 459
FspBI CTAG 2 cut(s) 222, 565
GluI GCNGC 3 cut(s) 43, 258, 459
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 2 cut(s) 31, 420
HaeIII GGCC 1 cut(s) 266
Hin1II CATG 8 cut(s) 70, 131, 241, 378, 416, 458, 513, 549
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 1 cut(s) 148
HphI GGTGA 2 cut(s) 317, 416
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 3 cut(s) 122, 208, 294
Hpy188III TCNNGA 3 cut(s) 128, 320, 449
Hpy8I GTNNAC 1 cut(s) 372
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 2 cut(s) 213, 315
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 3 cut(s) 412, 416, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 263
HpyF3I CTNAG 2 cut(s) 291, 419
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 8 cut(s) 70, 131, 241, 378, 416, 458, 513, 549
Kzo9I GATC 3 cut(s) 124, 322, 406
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 3 cut(s) 54, 269, 445
LweI GCATC 1 cut(s) 126
MaeI CTAG 2 cut(s) 222, 565
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 23
MalI GATC 3 cut(s) 126, 324, 408
MboI GATC 3 cut(s) 124, 322, 406
MboII GAAGA 1 cut(s) 178
MhlI GDGCHC 2 cut(s) 221, 292
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 2 cut(s) 108, 523
MluNI TGGCCA 1 cut(s) 266
MnlI CCTC 2 cut(s) 279, 395
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 2 cut(s) 108, 152
MscI TGGCCA 1 cut(s) 266
Msp20I TGGCCA 1 cut(s) 266
MspR9I CCNGG 3 cut(s) 399, 435, 463
MvaI CCWGG 3 cut(s) 399, 435, 463
MwoI GCNNNNNNNGC 2 cut(s) 113, 263
NdeII GATC 3 cut(s) 124, 322, 406
NlaIII CATG 8 cut(s) 70, 131, 241, 378, 416, 458, 513, 549
NmuCI GTSAC 1 cut(s) 23
NspI RCATGY 5 cut(s) 70, 378, 416, 458, 513
PaeI GCATGC 2 cut(s) 416, 458
PagI TCATGA 1 cut(s) 127
PciI ACATGT 2 cut(s) 66, 509
PdmI GAANNNNTTC 2 cut(s) 108, 152
PfeI GAWTC 1 cut(s) 148
PkrI GCNGC 3 cut(s) 44, 259, 460
PscI ACATGT 2 cut(s) 66, 509
PsiI TTATAA 1 cut(s) 500
Psp6I CCWGG 3 cut(s) 397, 433, 461
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 3 cut(s) 397, 433, 461
PstNI CAGNNNCTG 1 cut(s) 319
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SatI GCNGC 3 cut(s) 43, 258, 459
Sau3AI GATC 3 cut(s) 124, 322, 406
ScrFI CCNGG 3 cut(s) 399, 435, 463
SduI GDGCHC 2 cut(s) 221, 292
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 311
SphI GCATGC 2 cut(s) 416, 458
Sse9I AATT 2 cut(s) 108, 523
SspMI CTAG 2 cut(s) 222, 565
StyD4I CCNGG 3 cut(s) 397, 433, 461
TaaI ACNGT 2 cut(s) 213, 315
TaiI ACGT 1 cut(s) 30
TasI AATT 2 cut(s) 108, 523
TatI WGTACW 1 cut(s) 69
TfiI GAWTC 1 cut(s) 148
TscAI CASTG 3 cut(s) 22, 199, 267
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 42, 257, 458
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 1 cut(s) 254
TspRI CASTG 3 cut(s) 22, 199, 267
XceI RCATGY 5 cut(s) 70, 378, 416, 458, 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmnI GAANNNNTTC 2 cut(s) 108, 152
XspI CTAG 2 cut(s) 222, 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.