Rmu_sc0041306.1_g000002
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0041306.1
OG0017638
Physical Location & Seq
Reverse (-)
10605 .. 11546
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0041306.1_g000002.1.cds

Sequence Viewer

Length: 942 bp
atggaggtatggcttactcttaaacaaagatatgctagtacttcagagttgcatattatgaaagtgaagtctacattacaaagtattcagaagggttccgactatatttttctatatttgttgaaatttaagagtgctagagatgaattagcttctgttggtgttagaatatcagatcaagatgttaaaggtttgattcttgctggtcttcctattgaatatggtcccactagacagattatcagaggcaaaaatgatattggaatggaggaagttaggtctttgttattatctgctgagtatgagattgaattaaagcataaagctttgtcattgtccactcttgctgctatgattgcacaaaatagtcttgccaattcatctcaaagtatgcaatatgggatatcaagtacagggacatcatctcaagccatctttccatctcattatattggaaattgttctatgatacatgctctacccactgctgcatcaaatgttggttccttgcctaatgttaccaatactaatatgattcctggtatacctgcttataaccattacgatacctcatcttgtgcctctaattctcatcattataatgctccatcacctagctctactacactttttgggataccacagaatagcatgttatcaagctcagctcttagtgtgcctaatgctcctccaatttgtcaagacatgctcccattcactaccaatgttggtgttccacagtcaatatctaattctggatatatgctgcataatagtgttggaaatggaaattacttttcatcatctgcttcctcttatggttatgcacctcaaaatgataattctctggttcaaaatggtggatttatggcccaaaatggtggacacatagctcaaaatagtggttatgctcctactcttcaccacaactattcgcagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

313

Amino Acids

33.62

Weight (kDa)

6.82

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 555, 600
Acc36I ACCTGC 1 cut(s) 556
AccI GTMKAC 2 cut(s) 71, 544
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 2 cut(s) 40, 412
AgsI TTSAA 4 cut(s) 124, 218, 311, 854
AjnI CCWGG 1 cut(s) 538
AluBI AGCT 6 cut(s) 152, 326, 618, 663, 668, 893
AluI AGCT 6 cut(s) 152, 326, 618, 663, 668, 893
AoxI GGCC 1 cut(s) 870
ApeKI GCWGC 3 cut(s) 347, 488, 766
ApoI RAATTY 1 cut(s) 125
Asp700I GAANNNNTTC 1 cut(s) 460
AspS9I GGNCC 2 cut(s) 224, 871
AsuHPI GGTGA 2 cut(s) 603, 914
AvaII GGWCC 1 cut(s) 224
BbsI GAAGAC 1 cut(s) 200
BbvI GCAGC 3 cut(s) 334, 475, 753
BccI CCATC 3 cut(s) 440, 448, 616
BciT130I CCWGG 1 cut(s) 540
BciVI GTATCC 1 cut(s) 630
BfaI CTAG 4 cut(s) 36, 138, 231, 615
BfuAI ACCTGC 1 cut(s) 556
BfuI GTATCC 1 cut(s) 630
BisI GCNGC 3 cut(s) 348, 489, 767
BlpI GCTNAGC 1 cut(s) 664
BlsI GCNGC 3 cut(s) 349, 490, 768
BmcAI AGTACT 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 540
Bme18I GGWCC 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 224, 871
BmiI GGNNCC 3 cut(s) 97, 226, 505
BmrFI CCNGG 1 cut(s) 540
BmsI GCATC 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 664
BpuEI CTTGAG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 540
BseMII CTCAG 2 cut(s) 288, 678
BseRI GAGGAG 1 cut(s) 678
BseXI GCAGC 3 cut(s) 334, 475, 753
BshFI GGCC 1 cut(s) 872
BslFI GGGAC 2 cut(s) 210, 430
BsmFI GGGAC 2 cut(s) 210, 430
BsnI GGCC 1 cut(s) 872
Bsp143I GATC 1 cut(s) 175
Bsp1720I GCTNAGC 1 cut(s) 664
BspANI GGCC 1 cut(s) 872
BspCNI CTCAG 2 cut(s) 289, 677
BspLI GGNNCC 3 cut(s) 97, 226, 505
BspMI ACCTGC 1 cut(s) 556
BssMI GATC 1 cut(s) 175
BssNAI GTATAC 1 cut(s) 545
Bst1107I GTATAC 1 cut(s) 545
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 1 cut(s) 741
Bst6I CTCTTC 1 cut(s) 924
BstDEI CTNAG 3 cut(s) 297, 664, 671
BstKTI GATC 1 cut(s) 178
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNI CCWGG 1 cut(s) 540
BstNSI RCATGY 3 cut(s) 476, 655, 709
BstSCI CCNGG 1 cut(s) 538
BstV1I GCAGC 3 cut(s) 334, 475, 753
BstV2I GAAGAC 1 cut(s) 200
BstXI CCANNNNNNTGG 1 cut(s) 881
BstZ17I GTATAC 1 cut(s) 545
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 1 cut(s) 872
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 1 cut(s) 483
BveI ACCTGC 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 224, 871
Csp6I GTAC 2 cut(s) 39, 411
CviAII CATG 3 cut(s) 473, 652, 706
CviJI RGCY 9 cut(s) 13, 152, 326, 431, 618, 663, 668, 872, 893
CviKI_1 RGCY 9 cut(s) 13, 152, 326, 431, 618, 663, 668, 872, 893
CviQI GTAC 2 cut(s) 39, 411
DdeI CTNAG 3 cut(s) 297, 664, 671
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 924
EarI CTCTTC 1 cut(s) 924
Eco32I GATATC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 538
EcoRV GATATC 1 cut(s) 405
FaeI CATG 3 cut(s) 476, 655, 709
FaqI GGGAC 2 cut(s) 210, 430
FatI CATG 3 cut(s) 472, 651, 705
FblI GTMKAC 2 cut(s) 71, 544
Fnu4HI GCNGC 3 cut(s) 348, 489, 767
Fsp4HI GCNGC 3 cut(s) 348, 489, 767
FspBI CTAG 4 cut(s) 36, 138, 231, 615
GluI GCNGC 3 cut(s) 348, 489, 767
HaeIII GGCC 1 cut(s) 872
Hin1II CATG 3 cut(s) 476, 655, 709
HindIII AAGCTT 1 cut(s) 324
HinfI GANTC 2 cut(s) 196, 535
HphI GGTGA 2 cut(s) 603, 914
Hpy166II GTNNAC 4 cut(s) 72, 339, 545, 884
Hpy188I TCNGA 5 cut(s) 46, 90, 100, 175, 245
Hpy188III TCNNGA 3 cut(s) 179, 701, 756
Hpy8I GTNNAC 4 cut(s) 72, 339, 545, 884
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 741
HpyCH4V TGCA 6 cut(s) 52, 359, 394, 491, 769, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 3 cut(s) 297, 664, 671
Hsp92II CATG 3 cut(s) 476, 655, 709
Kzo9I GATC 1 cut(s) 175
LmnI GCTCC 4 cut(s) 610, 691, 714, 916
LpnPI CCDG 7 cut(s) 189, 399, 525, 552, 561, 741, 833
Lsp1109I GCAGC 3 cut(s) 334, 475, 753
LweI GCATC 1 cut(s) 500
MaeI CTAG 4 cut(s) 36, 138, 231, 615
MaeIII GTNAC 1 cut(s) 517
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 2 cut(s) 200, 911
MmeI TCCRAC 2 cut(s) 123, 760
MnlI CCTC 7 cut(s) 239, 262, 580, 592, 699, 823, 840
MroXI GAANNNNTTC 1 cut(s) 460
MseI TTAA 4 cut(s) 21, 129, 186, 314
MslI CAYNNNNRTG 2 cut(s) 600, 774
MspR9I CCNGG 1 cut(s) 540
MvaI CCWGG 1 cut(s) 540
MwoI GCNNNNNNNGC 1 cut(s) 356
NdeII GATC 1 cut(s) 175
NlaIII CATG 3 cut(s) 476, 655, 709
NlaIV GGNNCC 3 cut(s) 97, 226, 505
NspI RCATGY 3 cut(s) 476, 655, 709
PdmI GAANNNNTTC 1 cut(s) 460
PfeI GAWTC 2 cut(s) 196, 535
PkrI GCNGC 3 cut(s) 349, 490, 768
PsiI TTATAA 2 cut(s) 555, 600
Psp6I CCWGG 1 cut(s) 538
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 3 cut(s) 97, 226, 505
PspPI GGNCC 2 cut(s) 224, 871
RsaI GTAC 2 cut(s) 40, 412
RsaNI GTAC 2 cut(s) 39, 411
RseI CAYNNNNRTG 2 cut(s) 600, 774
SaqAI TTAA 4 cut(s) 21, 129, 186, 314
SatI GCNGC 3 cut(s) 348, 489, 767
Sau3AI GATC 1 cut(s) 175
Sau96I GGNCC 2 cut(s) 224, 871
ScaI AGTACT 1 cut(s) 40
ScrFI CCNGG 1 cut(s) 540
SfaNI GCATC 1 cut(s) 500
SinI GGWCC 1 cut(s) 224
SmiMI CAYNNNNRTG 2 cut(s) 600, 774
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
SspMI CTAG 4 cut(s) 36, 138, 231, 615
StyD4I CCNGG 1 cut(s) 538
TaaI ACNGT 1 cut(s) 741
TatI WGTACW 2 cut(s) 38, 410
TfiI GAWTC 2 cut(s) 196, 535
Tru1I TTAA 4 cut(s) 21, 129, 186, 314
Tru9I TTAA 4 cut(s) 21, 129, 186, 314
TscAI CASTG 1 cut(s) 490
TseI GCWGC 3 cut(s) 347, 488, 766
TspDTI ATGAA 4 cut(s) 74, 159, 369, 789
TspRI CASTG 1 cut(s) 490
VpaK11BI GGWCC 1 cut(s) 224
XapI RAATTY 1 cut(s) 125
XceI RCATGY 3 cut(s) 476, 655, 709
XmiI GTMKAC 2 cut(s) 71, 544
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 4 cut(s) 36, 138, 231, 615
ZrmI AGTACT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.