Rmu_sc0003275.1_g000022

MINDY deubiquitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003275.1
OG0017638
Physical Location & Seq
Forward (+)
73792 .. 74529
738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003275.1_g000022.1.cds

Sequence Viewer

Length: 738 bp
atgtttggcaccatctctggaagtaaacctgcatttgacactaatagtctcccaacctttggttcaatgaataccacagcctttggtacctacaccaccccagcttttggtcaatgtagctcgacttttggtactacaacaagtgttcctgcatttggccaatcaagttcaacctttggtagcacccaatttggcgcttcatctcctaatttcgttgaaactactccagggaaaacaacattttcatcccgattggagaccactgtggtgaacagtaatgatcagcctacttctgtgacatcatcagaagatctttctaaagatctggttacaaaaagtacagggaacacatcagaccaaattaaaggtgtagtttcttttagtcctgagagtgacgatgattccatggatgtgaaccatacagatatttcaaaaaggggtgaaaaatttgaggatcaggtcaaggttgcaactaatgccgatggaattgtagaaataaataagcaaaataggagtaatatgacagaggttcctttcatagaagcagaaactgaaagtgatgttagtgatgagagcagcttcagttgtttgagcaaaaacatgtacaagccccacgaggcgaatgatggaagttggattgacaatttgttgataaaaggcacatggcatcttccttgtacttctcttgaaagacctctactgaactgtaggcaggcttgtcggcctatgaagctatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MINDY_DUB

Protein Analysis

245

Amino Acids

26.43

Weight (kDa)

4.69

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 37
Acc65I GGTACC 1 cut(s) 86
AccB1I GGYRCC 2 cut(s) 8, 86
AccB7I CCANNNNNTGG 2 cut(s) 59, 107
AclWI GGATC 1 cut(s) 462
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 446
AcuI CTGAAG 1 cut(s) 565
AfaI GTAC 5 cut(s) 88, 133, 340, 605, 679
AfiI CCNNNNNNNGG 3 cut(s) 59, 107, 155
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 5 cut(s) 66, 171, 218, 432, 689
AjnI CCWGG 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AleI CACNNNNGTG 1 cut(s) 266
AluBI AGCT 4 cut(s) 104, 120, 579, 733
AluI AGCT 4 cut(s) 104, 120, 579, 733
Alw26I GTCTC 2 cut(s) 53, 251
AlwI GGATC 1 cut(s) 462
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 2 cut(s) 157, 722
ApeKI GCWGC 1 cut(s) 576
ApoI RAATTY 1 cut(s) 446
Asp718I GGTACC 1 cut(s) 86
AspLEI GCGC 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 280, 452
BalI TGGCCA 1 cut(s) 159
BanI GGYRCC 2 cut(s) 8, 86
BauI CACGAG 1 cut(s) 614
BbvI GCAGC 1 cut(s) 588
BccI CCATC 3 cut(s) 20, 476, 620
BcgI CGANNNNNNTGC 2 cut(s) 701, 735
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 53, 251
BfmI CTRYAG 1 cut(s) 706
BfoI RGCGCY 1 cut(s) 198
BfuAI ACCTGC 1 cut(s) 37
BglII AGATCT 2 cut(s) 310, 322
BisI GCNGC 1 cut(s) 577
BlsI GCNGC 1 cut(s) 578
Bme1390I CCNGG 1 cut(s) 228
BmiI GGNNCC 3 cut(s) 10, 88, 531
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 676
BpmI CTGGAG 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 227, 405
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 107, 155
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 227, 405
BseGI GGATG 2 cut(s) 245, 415
BseLI CCNNNNNNNGG 3 cut(s) 59, 107, 155
BseMII CTCAG 1 cut(s) 378
BseXI GCAGC 1 cut(s) 588
BseYI CCCAGC 1 cut(s) 100
BshFI GGCC 2 cut(s) 159, 724
BshNI GGYRCC 2 cut(s) 8, 86
BslI CCNNNNNNNGG 3 cut(s) 59, 107, 155
BsmAI GTCTC 2 cut(s) 53, 251
BsnI GGCC 2 cut(s) 159, 724
Bso31I GGTCTC 1 cut(s) 251
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 4 cut(s) 280, 310, 322, 454
Bsp19I CCATGG 1 cut(s) 405
BspANI GGCC 2 cut(s) 159, 724
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 3 cut(s) 10, 88, 531
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 1 cut(s) 462
BspT107I GGYRCC 2 cut(s) 8, 86
BspTNI GGTCTC 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 603
BssECI CCNNGG 2 cut(s) 227, 405
BssMI GATC 4 cut(s) 280, 310, 322, 454
BssSI CACGAG 1 cut(s) 614
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 614
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 3 cut(s) 265, 275, 707
BstAPI GCANNNNNTGC 1 cut(s) 476
BstAUI TGTACA 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 245, 415
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 4 cut(s) 283, 313, 325, 457
BstMAI GTCTC 2 cut(s) 53, 251
BstMBI GATC 4 cut(s) 280, 310, 322, 454
BstMWI GCNNNNNNNGC 2 cut(s) 476, 730
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 706
BstV1I GCAGC 1 cut(s) 588
BstX2I RGATCY 2 cut(s) 310, 322
BstYI RGATCY 2 cut(s) 310, 322
BsuRI GGCC 2 cut(s) 159, 724
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 2 cut(s) 245, 415
BtsIMutI CAGTG 1 cut(s) 261
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 714
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 1 cut(s) 197
Csp6I GTAC 5 cut(s) 87, 132, 339, 604, 678
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviAII CATG 3 cut(s) 406, 601, 663
CviQI GTAC 5 cut(s) 87, 132, 339, 604, 678
DdeI CTNAG 1 cut(s) 387
DpnI GATC 4 cut(s) 282, 312, 324, 456
DpnII GATC 4 cut(s) 280, 310, 322, 454
EaeI YGGCCR 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 3 cut(s) 409, 604, 666
FaiI YATR 8 cut(s) 407, 420, 521, 539, 602, 664, 728, 736
FatI CATG 3 cut(s) 405, 600, 662
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 577
FokI GGATG 2 cut(s) 232, 422
Fsp4HI GCNGC 1 cut(s) 577
GlaI GCGC 1 cut(s) 196
GluI GCNGC 1 cut(s) 577
GsaI CCCAGC 1 cut(s) 104
GsuI CTGGAG 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 2 cut(s) 159, 724
HhaI GCGC 1 cut(s) 197
Hin1II CATG 3 cut(s) 409, 604, 666
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HinfI GANTC 1 cut(s) 401
HphI GGTGA 2 cut(s) 280, 452
Hpy166II GTNNAC 3 cut(s) 26, 271, 415
Hpy188I TCNGA 2 cut(s) 307, 355
Hpy188III TCNNGA 4 cut(s) 18, 249, 386, 686
Hpy8I GTNNAC 3 cut(s) 26, 271, 415
HpyCH4III ACNGT 3 cut(s) 265, 275, 707
HpyCH4V TGCA 3 cut(s) 32, 152, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 730
HpyF3I CTNAG 1 cut(s) 387
Hsp92II CATG 3 cut(s) 409, 604, 666
HspAI GCGC 1 cut(s) 195
KpnI GGTACC 1 cut(s) 90
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 4 cut(s) 280, 310, 322, 454
Lsp1109I GCAGC 1 cut(s) 588
LweI GCATC 1 cut(s) 676
MaeIII GTNAC 3 cut(s) 295, 328, 392
MalI GATC 4 cut(s) 282, 312, 324, 456
MboI GATC 4 cut(s) 280, 310, 322, 454
MboII GAAGA 2 cut(s) 320, 662
MflI RGATCY 2 cut(s) 310, 322
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 6 cut(s) 188, 208, 360, 446, 486, 643
MluNI TGGCCA 1 cut(s) 159
MmeI TCCRAC 1 cut(s) 614
MnlI CCTC 4 cut(s) 445, 520, 610, 705
Mox20I TGGCCA 1 cut(s) 159
MscI TGGCCA 1 cut(s) 159
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 266, 410
Msp20I TGGCCA 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 476, 730
NcoI CCATGG 1 cut(s) 405
NdeII GATC 4 cut(s) 280, 310, 322, 454
NlaIII CATG 3 cut(s) 409, 604, 666
NlaIV GGNNCC 3 cut(s) 10, 88, 531
NmuCI GTSAC 2 cut(s) 295, 392
NspI RCATGY 1 cut(s) 604
OliI CACNNNNGTG 1 cut(s) 266
PciI ACATGT 1 cut(s) 600
PfeI GAWTC 1 cut(s) 401
PflMI CCANNNNNTGG 2 cut(s) 59, 107
PkrI GCNGC 1 cut(s) 578
PscI ACATGT 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 3 cut(s) 10, 88, 531
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 2 cut(s) 310, 322
RsaI GTAC 5 cut(s) 88, 133, 340, 605, 679
RsaNI GTAC 5 cut(s) 87, 132, 339, 604, 678
RseI CAYNNNNRTG 2 cut(s) 266, 410
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 1 cut(s) 577
Sau3AI GATC 4 cut(s) 280, 310, 322, 454
ScrFI CCNGG 1 cut(s) 228
SfaNI GCATC 1 cut(s) 676
SfcI CTRYAG 1 cut(s) 706
SmiMI CAYNNNNRTG 2 cut(s) 266, 410
Sse9I AATT 6 cut(s) 188, 208, 360, 446, 486, 643
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 3 cut(s) 265, 275, 707
TaqI TCGA 1 cut(s) 122
TasI AATT 6 cut(s) 188, 208, 360, 446, 486, 643
TatI WGTACW 3 cut(s) 338, 603, 677
TfiI GAWTC 1 cut(s) 401
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 2 cut(s) 295, 392
TseI GCWGC 1 cut(s) 576
Tsp45I GTSAC 2 cut(s) 295, 392
TspDTI ATGAA 4 cut(s) 83, 189, 234, 526
TspRI CASTG 1 cut(s) 268
Van91I CCANNNNNTGG 2 cut(s) 59, 107
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.