Rmu_sc0000051.1_g000003

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000051.1
OG0007935
Physical Location & Seq
Reverse (-)
7610 .. 9877
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 966 bp
atgctcgtgctacaagcagctgtcaagatggtatggagtctacctgaagttatgagggtagcatatgcattcatgcttattatgcttttatggatgggaatatggtcctttggggctgctggtgttgtagctttaagcattggagatggtggacgctggtggcttcttgtggttctctctgtaagcttattttggacgggtgctgttctctgtaatactgtacatgttatagtgtctgggatggtgtttcttgttctttcccatggtggtcgagaggcctcatcaatgcctccgaagtcactaatgaagtctcttcggtatgcagtcacaacttcttttggcagcatctgctatggttcacttttcacagctgccattcgaactttgcgctggaagatcaggggagttcggtccaagattggcaacaatgagtgtctactttgctgtgttgatttcctctttcatcttgtggagacacttgtgcgattctttaataagtatgcttacgtccagatagctgtttctggcaaaggtttcaatcattcagcgagggatgcttgggagttatttcagtctactggagtcgaggcactcattgcctatgattgctcaggtgctgtattattgatgggcacactgttgggtgggcttattaccggaacttgctcggggatctggacatggattaaatatagggacagagtggctatggttgggtccactgcaatgttgatgggaatgatcctggtgggattagcaatggtggttgtggaaagtgctgtcacatctatctacatctgttatgcagaagacccgttgttgattcataggtgggatactgaattcttcaaccagatgtcggagatgctacaccagcgtcttcaacataggagtgcccgggcaagggaagtgttgacccacaatcgggttgataaccaccacacactcgaaacacttcctttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

321

Amino Acids

35.79

Weight (kDa)

8.93

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 2 - 272 1.1e-53 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 40, 436, 575
AclWI GGATC 2 cut(s) 680, 736
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 1 cut(s) 222
AfiI CCNNNNNNNGG 5 cut(s) 859, 903, 904, 924, 925
AflIII ACRYGT 1 cut(s) 223
AgsI TTSAA 3 cut(s) 538, 850, 884
AjnI CCWGG 1 cut(s) 744
AluBI AGCT 5 cut(s) 20, 131, 186, 371, 518
AluI AGCT 5 cut(s) 20, 131, 186, 371, 518
Alw26I GTCTC 2 cut(s) 315, 467
AlwI GGATC 2 cut(s) 680, 736
AlwNI CAGNNNCTG 2 cut(s) 348, 617
Ama87I CYCGRG 2 cut(s) 667, 897
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 4 cut(s) 17, 116, 342, 371
ApoI RAATTY 1 cut(s) 842
Asp700I GAANNNNTTC 1 cut(s) 954
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 3 cut(s) 105, 411, 717
AsuC2I CCSGG 2 cut(s) 898, 899
AsuII TTCGAA 1 cut(s) 379
AvaI CYCGRG 2 cut(s) 667, 897
AvaII GGWCC 3 cut(s) 105, 411, 717
BaeGI GKGCMC 2 cut(s) 635, 898
BauI CACGAG 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 816, 872
BbvI GCAGC 4 cut(s) 29, 103, 354, 358
BccI CCATC 6 cut(s) 22, 88, 140, 235, 622, 727
BciT130I CCWGG 1 cut(s) 746
BciVI GTATCC 1 cut(s) 829
BcnI CCSGG 2 cut(s) 898, 899
BcoDI GTCTC 2 cut(s) 315, 467
BfuI GTATCC 1 cut(s) 829
BisI GCNGC 4 cut(s) 18, 117, 343, 372
BlsI GCNGC 4 cut(s) 19, 118, 344, 373
Bme1390I CCNGG 3 cut(s) 746, 898, 899
Bme18I GGWCC 3 cut(s) 105, 411, 717
BmeT110I CYCGRG 2 cut(s) 667, 897
BmgT120I GGNCC 3 cut(s) 105, 411, 717
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 3 cut(s) 746, 898, 899
BmsI GCATC 3 cut(s) 354, 544, 855
BpiI GAAGAC 2 cut(s) 816, 872
BpmI CTGGAG 1 cut(s) 600
Bpu10I CCTNAGC 1 cut(s) 610
Bpu14I TTCGAA 1 cut(s) 379
BpuMI CCSGG 2 cut(s) 898, 899
BsaJI CCNNGG 2 cut(s) 262, 897
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 5 cut(s) 859, 903, 904, 924, 925
Bse1I ACTGG 1 cut(s) 583
Bse3DI GCAATG 3 cut(s) 594, 732, 765
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 2 cut(s) 262, 897
BseGI GGATG 3 cut(s) 99, 246, 559
BseLI CCNNNNNNNGG 5 cut(s) 859, 903, 904, 924, 925
BseMI GCAATG 3 cut(s) 594, 732, 765
BseMII CTCAG 1 cut(s) 624
BseNI ACTGG 1 cut(s) 583
BseSI GKGCMC 2 cut(s) 635, 898
BseXI GCAGC 4 cut(s) 29, 103, 354, 358
BshFI GGCC 1 cut(s) 278
BsiHKCI CYCGRG 2 cut(s) 667, 897
BsiSI CCGG 2 cut(s) 657, 898
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 5 cut(s) 859, 903, 904, 924, 925
BsmAI GTCTC 2 cut(s) 315, 467
BsmFI GGGAC 1 cut(s) 710
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 1 cut(s) 278
BsoBI CYCGRG 2 cut(s) 667, 897
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 635, 898
Bsp1407I TGTACA 1 cut(s) 220
Bsp143I GATC 3 cut(s) 396, 672, 741
Bsp19I CCATGG 1 cut(s) 262
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 1 cut(s) 623
BspLI GGNNCC 1 cut(s) 718
BspPI GGATC 2 cut(s) 680, 736
BspT104I TTCGAA 1 cut(s) 379
BsrDI GCAATG 3 cut(s) 594, 732, 765
BsrGI TGTACA 1 cut(s) 220
BsrI ACTGG 1 cut(s) 583
BssECI CCNNGG 2 cut(s) 262, 897
BssMI GATC 3 cut(s) 396, 672, 741
BssSI CACGAG 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 5
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 2 cut(s) 220, 639
Bst6I CTCTTC 1 cut(s) 318
BstAPI GCANNNNNTGC 2 cut(s) 348, 596
BstAUI TGTACA 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 379
BstDEI CTNAG 1 cut(s) 610
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 3 cut(s) 99, 246, 559
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 3 cut(s) 399, 675, 744
BstMAI GTCTC 2 cut(s) 315, 467
BstMBI GATC 3 cut(s) 396, 672, 741
BstMWI GCNNNNNNNGC 5 cut(s) 82, 348, 554, 596, 874
BstNI CCWGG 1 cut(s) 746
BstNSI RCATGY 1 cut(s) 227
BstSCI CCNGG 3 cut(s) 744, 896, 897
BstSLI GKGCMC 2 cut(s) 635, 898
BstV1I GCAGC 4 cut(s) 29, 103, 354, 358
BstV2I GAAGAC 2 cut(s) 816, 872
BstX2I RGATCY 1 cut(s) 672
BstYI RGATCY 1 cut(s) 672
BsuI GTATCC 1 cut(s) 829
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 262
BtsCI GGATG 3 cut(s) 99, 246, 559
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 635, 720
CaiI CAGNNNCTG 2 cut(s) 348, 617
CfoI GCGC 1 cut(s) 390
Cfr13I GGNCC 3 cut(s) 105, 411, 717
Cfr9I CCCGGG 1 cut(s) 897
CseI GACGC 2 cut(s) 162, 866
Csp6I GTAC 1 cut(s) 221
CviAII CATG 4 cut(s) 73, 224, 263, 681
CviQI GTAC 1 cut(s) 221
DdeI CTNAG 1 cut(s) 610
DpnI GATC 3 cut(s) 398, 674, 743
DpnII GATC 3 cut(s) 396, 672, 741
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 262
Eco147I AGGCCT 1 cut(s) 278
Eco47I GGWCC 3 cut(s) 105, 411, 717
Eco57I CTGAAG 1 cut(s) 66
Eco88I CYCGRG 2 cut(s) 667, 897
EcoRI GAATTC 1 cut(s) 842
EcoRII CCWGG 1 cut(s) 744
EcoT14I CCWWGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 4 cut(s) 76, 227, 266, 684
FaqI GGGAC 1 cut(s) 710
FatI CATG 4 cut(s) 72, 223, 262, 680
FauNDI CATATG 1 cut(s) 64
FblI GTMKAC 3 cut(s) 40, 436, 575
Fnu4HI GCNGC 4 cut(s) 18, 117, 343, 372
FokI GGATG 3 cut(s) 106, 253, 566
Fsp4HI GCNGC 4 cut(s) 18, 117, 343, 372
GlaI GCGC 1 cut(s) 389
GluI GCNGC 4 cut(s) 18, 117, 343, 372
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 1 cut(s) 278
HapII CCGG 2 cut(s) 657, 898
HgaI GACGC 2 cut(s) 162, 866
HhaI GCGC 1 cut(s) 390
Hin1II CATG 4 cut(s) 76, 227, 266, 684
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HindIII AAGCTT 1 cut(s) 184
HinfI GANTC 4 cut(s) 37, 486, 582, 823
HpaII CCGG 2 cut(s) 657, 898
Hpy166II GTNNAC 7 cut(s) 41, 152, 359, 437, 576, 720, 915
Hpy188I TCNGA 2 cut(s) 294, 862
Hpy188III TCNNGA 4 cut(s) 25, 272, 511, 676
Hpy8I GTNNAC 7 cut(s) 41, 152, 359, 437, 576, 720, 915
HpyCH4III ACNGT 2 cut(s) 220, 639
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 4 cut(s) 68, 323, 725, 806
HpyF10VI GCNNNNNNNGC 5 cut(s) 82, 348, 554, 596, 874
HpyF3I CTNAG 1 cut(s) 610
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 4 cut(s) 76, 227, 266, 684
HspAI GCGC 1 cut(s) 388
Kzo9I GATC 3 cut(s) 396, 672, 741
Lsp1109I GCAGC 4 cut(s) 29, 103, 354, 358
LweI GCATC 3 cut(s) 354, 544, 855
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 3 cut(s) 297, 325, 781
MalI GATC 3 cut(s) 398, 674, 743
MboI GATC 3 cut(s) 396, 672, 741
MboII GAAGA 5 cut(s) 305, 406, 821, 838, 872
MflI RGATCY 1 cut(s) 672
MhlI GDGCHC 2 cut(s) 635, 898
MluCI AATT 1 cut(s) 842
MlyI GAGTC 2 cut(s) 46, 591
MmeI TCCRAC 1 cut(s) 840
MnlI CCTC 7 cut(s) 48, 268, 289, 300, 467, 543, 580
Mph1103I ATGCAT 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 954
MseI TTAA 3 cut(s) 134, 492, 687
MslI CAYNNNNRTG 2 cut(s) 725, 891
MspA1I CMGCKG 2 cut(s) 20, 371
MspI CCGG 2 cut(s) 657, 898
MspR9I CCNGG 3 cut(s) 746, 898, 899
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 1 cut(s) 746
MwoI GCNNNNNNNGC 5 cut(s) 82, 348, 554, 596, 874
NciI CCSGG 2 cut(s) 898, 899
NcoI CCATGG 1 cut(s) 262
NdeI CATATG 1 cut(s) 64
NdeII GATC 3 cut(s) 396, 672, 741
NlaIII CATG 4 cut(s) 76, 227, 266, 684
NlaIV GGNNCC 1 cut(s) 718
NmuCI GTSAC 3 cut(s) 297, 325, 781
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 1 cut(s) 227
NspV TTCGAA 1 cut(s) 379
PceI AGGCCT 1 cut(s) 278
PciI ACATGT 1 cut(s) 223
PctI GAATGC 1 cut(s) 68
PdmI GAANNNNTTC 1 cut(s) 954
PfeI GAWTC 2 cut(s) 486, 823
PkrI GCNGC 4 cut(s) 19, 118, 344, 373
PleI GAGTC 2 cut(s) 45, 590
PpsI GAGTC 2 cut(s) 45, 590
PscI ACATGT 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 744
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 3 cut(s) 105, 411, 717
PstNI CAGNNNCTG 2 cut(s) 348, 617
PsuI RGATCY 1 cut(s) 672
PvuII CAGCTG 2 cut(s) 20, 371
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
RseI CAYNNNNRTG 2 cut(s) 725, 891
SaqAI TTAA 3 cut(s) 134, 492, 687
SatI GCNGC 4 cut(s) 18, 117, 343, 372
Sau3AI GATC 3 cut(s) 396, 672, 741
Sau96I GGNCC 3 cut(s) 105, 411, 717
SchI GAGTC 2 cut(s) 46, 591
ScrFI CCNGG 3 cut(s) 746, 898, 899
SduI GDGCHC 2 cut(s) 635, 898
SfaNI GCATC 3 cut(s) 354, 544, 855
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 3 cut(s) 105, 411, 717
SmaI CCCGGG 1 cut(s) 899
SmiMI CAYNNNNRTG 2 cut(s) 725, 891
SrfI GCCCGGGC 1 cut(s) 899
Sse9I AATT 1 cut(s) 842
SseBI AGGCCT 1 cut(s) 278
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 3 cut(s) 744, 896, 897
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 2 cut(s) 220, 639
TaiI ACGT 1 cut(s) 510
TaqI TCGA 4 cut(s) 271, 379, 585, 948
TaqII GACCGA 1 cut(s) 399
TasI AATT 1 cut(s) 842
TatI WGTACW 1 cut(s) 220
TfiI GAWTC 2 cut(s) 486, 823
Tru1I TTAA 3 cut(s) 134, 492, 687
Tru9I TTAA 3 cut(s) 134, 492, 687
TscAI CASTG 2 cut(s) 642, 727
TseFI GTSAC 3 cut(s) 297, 325, 781
TseI GCWGC 4 cut(s) 17, 116, 342, 371
Tsp45I GTSAC 3 cut(s) 297, 325, 781
TspDTI ATGAA 4 cut(s) 61, 320, 452, 815
TspMI CCCGGG 1 cut(s) 897
TspRI CASTG 2 cut(s) 642, 727
VpaK11BI GGWCC 3 cut(s) 105, 411, 717
XapI RAATTY 1 cut(s) 842
XceI RCATGY 1 cut(s) 227
XmaI CCCGGG 1 cut(s) 897
XmiI GTMKAC 3 cut(s) 40, 436, 575
XmnI GAANNNNTTC 1 cut(s) 954
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.