Rmu_sc0000084.1_g000042

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000084.1
OG0014824
Physical Location & Seq
Reverse (-)
177222 .. 177941
720 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ctggctccttatacccttctccgtcttcctcactttcaccctaaagttcatcctcttcccctacaaaccctcccacaaactcaacgccccactccctccgggtcccaccaccatacccgtcgtcggcaccttcatatggctcttcaaactcgcctcccgcggcatcgagccagtccagacaaacttgtcacagaaattagacaaattgtgggagacggagaagttgaagaggtggaagaggagaacctgcacagaatgaagtatttaaaagcggttgtgctagaaggtctgcgtcttcacccaccgtcgcactttttgcttccgcactctgtcactgaggacgtcattttggacgggcgttacgttgtgccgaaggactgcactgtgaacttctctgtggcggagatggggcgggatccgaaggtgtgggcggagccgatggaattcaagcctgagaggtttctgagtgatgaggagaacttgtgtgatttaactggtagtaaggagatcaagatgatgccgtttggggtagggaggaggatgtgtcctggttcgaatttggcaatgctgcttttggagtactttgtggggaatttggtttggaaattcgagtggagagctgtggagggagttggggttgatttgagtgagaaggaggagacgagttgggggatgaataatccattgcaggcacatatcactccaaggttgaagacatga

Protein Analysis

239

Amino Acids

27.49

Weight (kDa)

7.0

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 51 - 222 7.4e-46 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 185
AatII GACGTC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 126
AccII CGCG 1 cut(s) 160
AciI CCGC 7 cut(s) 158, 160, 272, 323, 401, 412, 431
AclWI GGATC 2 cut(s) 410, 423
AcsI RAATTY 4 cut(s) 443, 556, 592, 605
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 2 cut(s) 123, 136
AgsI TTSAA 4 cut(s) 146, 227, 448, 712
AjnI CCWGG 1 cut(s) 547
AluBI AGCT 1 cut(s) 620
AluI AGCT 1 cut(s) 620
Alw26I GTCTC 2 cut(s) 207, 653
AlwI GGATC 2 cut(s) 410, 423
ApeKI GCWGC 1 cut(s) 568
ApoI RAATTY 4 cut(s) 443, 556, 592, 605
AspS9I GGNCC 1 cut(s) 102
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 2 cut(s) 29, 290
AsuII TTCGAA 1 cut(s) 554
AvaII GGWCC 1 cut(s) 102
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 17, 287
BbvI GCAGC 1 cut(s) 555
BccI CCATC 2 cut(s) 400, 433
BceAI ACGGC 1 cut(s) 505
BciT130I CCWGG 1 cut(s) 549
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 207, 653
BfaI CTAG 1 cut(s) 281
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 2 cut(s) 161, 569
BlsI GCNGC 2 cut(s) 162, 570
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 2 cut(s) 100, 549
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 6 cut(s) 6, 103, 104, 128, 417, 435
BmrFI CCNGG 2 cut(s) 100, 549
BmsI GCATC 2 cut(s) 172, 507
BpiI GAAGAC 2 cut(s) 17, 287
Bpu14I TTCGAA 1 cut(s) 554
BpuMI CCSGG 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 342
BsaJI CCNNGG 2 cut(s) 158, 704
BsaXI ACNNNNNCTCC 8 cut(s) 54, 84, 467, 497, 529, 559, 621, 651
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 136
Bse1I ACTGG 2 cut(s) 171, 499
Bse3DI GCAATG 2 cut(s) 570, 683
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 158, 704
BseGI GGATG 3 cut(s) 49, 546, 678
BseLI CCNNNNNNNGG 2 cut(s) 123, 136
BseMI GCAATG 2 cut(s) 570, 683
BseMII CTCAG 3 cut(s) 327, 444, 455
BseNI ACTGG 2 cut(s) 171, 499
BseRI GAGGAG 4 cut(s) 254, 488, 550, 671
BseXI GCAGC 1 cut(s) 555
BsgI GTGCAG 2 cut(s) 233, 364
Bsh1236I CGCG 1 cut(s) 160
BshNI GGYRCC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 2 cut(s) 123, 136
BsmAI GTCTC 2 cut(s) 207, 653
BsmBI CGTCTC 2 cut(s) 207, 653
BsmFI GGGAC 1 cut(s) 88
Bsp119I TTCGAA 1 cut(s) 554
Bsp143I GATC 2 cut(s) 415, 507
BspACI CCGC 7 cut(s) 158, 160, 272, 323, 401, 412, 431
BspCNI CTCAG 3 cut(s) 328, 445, 456
BspFNI CGCG 1 cut(s) 160
BspLI GGNNCC 6 cut(s) 6, 103, 104, 128, 417, 435
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 410, 423
BspQI GCTCTTC 1 cut(s) 147
BspT104I TTCGAA 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 126
BsrDI GCAATG 2 cut(s) 570, 683
BsrI ACTGG 2 cut(s) 171, 499
BssECI CCNNGG 2 cut(s) 158, 704
BssMI GATC 2 cut(s) 415, 507
BssNI GRCGYC 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 704
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 2 cut(s) 306, 385
Bst6I CTCTTC 4 cut(s) 60, 147, 222, 231
BstACI GRCGYC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 3 cut(s) 336, 453, 464
BstDSI CCRYGG 1 cut(s) 158
BstF5I GGATG 3 cut(s) 49, 546, 678
BstFNI CGCG 1 cut(s) 160
BstKTI GATC 2 cut(s) 418, 510
BstMAI GTCTC 2 cut(s) 207, 653
BstMBI GATC 2 cut(s) 415, 507
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNI CCWGG 1 cut(s) 549
BstSCI CCNGG 2 cut(s) 98, 547
BstUI CGCG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 555
BstV2I GAAGAC 2 cut(s) 17, 287
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BtgI CCRYGG 1 cut(s) 158
BtsCI GGATG 3 cut(s) 49, 546, 678
BtsIMutI CAGTG 2 cut(s) 333, 381
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 690
Cfr13I GGNCC 1 cut(s) 102
Cfr42I CCGCGG 1 cut(s) 161
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 580
CviAII CATG 1 cut(s) 717
CviJI RGCY 6 cut(s) 5, 140, 170, 436, 451, 620
CviKI_1 RGCY 6 cut(s) 5, 140, 170, 436, 451, 620
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 3 cut(s) 336, 453, 464
DpnI GATC 2 cut(s) 417, 509
DpnII GATC 2 cut(s) 415, 507
DraI TTTAAA 1 cut(s) 267
DrdI GACNNNNNNGTC 1 cut(s) 185
DseDI GACNNNNNNGTC 1 cut(s) 185
Eam1104I CTCTTC 4 cut(s) 60, 147, 222, 231
EarI CTCTTC 4 cut(s) 60, 147, 222, 231
EciI GGCGGA 2 cut(s) 416, 446
Eco130I CCWWGG 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 704
Esp3I CGTCTC 2 cut(s) 207, 653
FaeI CATG 1 cut(s) 720
FaiI YATR 6 cut(s) 12, 114, 135, 137, 696, 718
FaqI GGGAC 1 cut(s) 88
FatI CATG 1 cut(s) 716
FauI CCCGC 2 cut(s) 165, 405
FauNDI CATATG 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 161, 569
FokI GGATG 3 cut(s) 36, 553, 685
Fsp4HI GCNGC 2 cut(s) 161, 569
FspBI CTAG 1 cut(s) 281
GluI GCNGC 2 cut(s) 161, 569
HapII CCGG 1 cut(s) 99
HgaI GACGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 1 cut(s) 720
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 29, 290
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 2 cut(s) 420, 465
Hpy188III TCNNGA 2 cut(s) 176, 511
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 3 cut(s) 123, 126, 310
HpyAV CCTTC 6 cut(s) 26, 140, 278, 367, 415, 646
HpyCH4III ACNGT 2 cut(s) 306, 385
HpyCH4IV ACGT 2 cut(s) 342, 363
HpyCH4V TGCA 3 cut(s) 250, 381, 688
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 3 cut(s) 336, 453, 464
HpySE526I ACGT 2 cut(s) 342, 363
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 1 cut(s) 720
KflI GGGWCCC 1 cut(s) 102
KspI CCGCGG 1 cut(s) 161
Kzo9I GATC 2 cut(s) 415, 507
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 2 cut(s) 10, 433
LpnPI CCDG 9 cut(s) 112, 184, 189, 260, 465, 480, 534, 561, 674
Lsp1109I GCAGC 1 cut(s) 555
LweI GCATC 2 cut(s) 172, 507
MaeI CTAG 1 cut(s) 281
MaeII ACGT 2 cut(s) 342, 363
MaeIII GTNAC 3 cut(s) 187, 331, 359
MalI GATC 2 cut(s) 417, 509
MboI GATC 2 cut(s) 415, 507
MboII GAAGA 6 cut(s) 17, 47, 134, 239, 248, 287
MflI RGATCY 1 cut(s) 415
MluCI AATT 6 cut(s) 195, 204, 443, 556, 592, 605
MseI TTAA 2 cut(s) 266, 491
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 100, 549
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 316
NciI CCSGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 135
NdeII GATC 2 cut(s) 415, 507
NlaIII CATG 1 cut(s) 720
NlaIV GGNNCC 6 cut(s) 6, 103, 104, 128, 417, 435
NmuCI GTSAC 2 cut(s) 187, 331
NspV TTCGAA 1 cut(s) 554
PciSI GCTCTTC 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PkrI GCNGC 2 cut(s) 162, 570
PpuMI RGGWCCY 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 547
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 6 cut(s) 6, 103, 104, 128, 417, 435
PspPI GGNCC 1 cut(s) 102
PspPPI RGGWCCY 1 cut(s) 102
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
SacII CCGCGG 1 cut(s) 161
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 2 cut(s) 266, 491
SatI GCNGC 2 cut(s) 161, 569
Sau3AI GATC 2 cut(s) 415, 507
Sau96I GGNCC 1 cut(s) 102
ScaI AGTACT 1 cut(s) 581
ScrFI CCNGG 2 cut(s) 100, 549
SfaNI GCATC 2 cut(s) 172, 507
Sfr303I CCGCGG 1 cut(s) 161
SfuI TTCGAA 1 cut(s) 554
SgrBI CCGCGG 1 cut(s) 161
SinI GGWCC 1 cut(s) 102
Sse9I AATT 6 cut(s) 195, 204, 443, 556, 592, 605
SsiI CCGC 7 cut(s) 158, 160, 272, 323, 401, 412, 431
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 2 cut(s) 98, 547
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 2 cut(s) 306, 385
TaiI ACGT 2 cut(s) 345, 366
TaqI TCGA 3 cut(s) 166, 554, 609
TasI AATT 6 cut(s) 195, 204, 443, 556, 592, 605
TatI WGTACW 1 cut(s) 579
TauI GCSGC 1 cut(s) 163
Tru1I TTAA 2 cut(s) 266, 491
Tru9I TTAA 2 cut(s) 266, 491
TscAI CASTG 2 cut(s) 340, 388
TseFI GTSAC 2 cut(s) 187, 331
TseI GCWGC 1 cut(s) 568
Tsp45I GTSAC 2 cut(s) 187, 331
TspDTI ATGAA 4 cut(s) 38, 122, 272, 689
TspGWI ACGGA 2 cut(s) 11, 231
TspRI CASTG 2 cut(s) 340, 388
VpaK11BI GGWCC 1 cut(s) 102
XapI RAATTY 4 cut(s) 443, 556, 592, 605
XspI CTAG 1 cut(s) 281
ZraI GACGTC 1 cut(s) 343
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.