Rh2BG374100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014824
Physical Location & Seq
Forward (+)
52651450 .. 52651779
330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG374100.1

Sequence Viewer

Length: 330 bp
ATGGTGGCCGAAATTGGTCGAGACTCGAGTTTGTGGGACGAGCCCATGGAGTTTAAACCTGAAAGGTTTATGAAAGATGGAGGTAACGTACAATGTGATATAACAGGGAGCAAGGAGATTATGATGATACCGTTTGGGGCGGGTAGGAGAATTTGTCCGGGATTGGGTTTGTCAATGCTTCTTTTGGAGTATTTGGTGGCAAATCTGGTGTGGAAGTACGAGTGGGAAGCTGTGGATGGAGTTGGTGTTGATTTGACTGAGAGGCAAGAGGCCACCTGGGTAATGAAGAATCCACTACAGGCTCATATATCTCCAAGGTTGAACAAATGA

Protein Analysis

109

Amino Acids

12.36

Weight (kDa)

4.95

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 4 - 97 4.8e-18 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 150
AfaI GTAC 2 cut(s) 90, 218
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 1 cut(s) 322
AjnI CCWGG 1 cut(s) 275
AluBI AGCT 1 cut(s) 230
AluI AGCT 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 15
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 2 cut(s) 6, 270
ApoI RAATTY 1 cut(s) 150
AsuC2I CCSGG 1 cut(s) 159
AvaI CYCGRG 1 cut(s) 25
BanII GRGCYC 1 cut(s) 45
BccI CCATC 2 cut(s) 71, 230
BciT130I CCWGG 1 cut(s) 277
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 296
Bme1390I CCNGG 2 cut(s) 159, 277
BmeT110I CYCGRG 1 cut(s) 25
BmrFI CCNGG 2 cut(s) 159, 277
BpuMI CCSGG 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 45, 276, 314
BsaXI ACNNNNNCTCC 6 cut(s) 72, 102, 139, 169, 231, 261
Bsc4I CCNNNNNNNGG 1 cut(s) 164
BseBI CCWGG 1 cut(s) 277
BseDI CCNNGG 3 cut(s) 45, 276, 314
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 1 cut(s) 249
BshFI GGCC 2 cut(s) 8, 272
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 50
BsnI GGCC 2 cut(s) 8, 272
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 45
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 1 cut(s) 140
BspANI GGCC 2 cut(s) 8, 272
BspCNI CTCAG 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 45, 276, 314
BssT1I CCWWGG 2 cut(s) 45, 314
Bst2UI CCWGG 1 cut(s) 277
Bst4CI ACNGT 1 cut(s) 132
BstDEI CTNAG 1 cut(s) 258
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 241
BstMAI GTCTC 1 cut(s) 15
BstNI CCWGG 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 157, 275
BstSFI CTRYAG 1 cut(s) 296
BsuRI GGCC 2 cut(s) 8, 272
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 241
Csp6I GTAC 2 cut(s) 89, 217
CviAII CATG 1 cut(s) 46
CviJI RGCY 5 cut(s) 8, 43, 230, 272, 302
CviKI_1 RGCY 5 cut(s) 8, 43, 230, 272, 302
CviQI GTAC 2 cut(s) 89, 217
DdeI CTNAG 1 cut(s) 258
DraI TTTAAA 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 45, 314
Eco24I GRGCYC 1 cut(s) 45
Eco88I CYCGRG 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 275
EcoT14I CCWWGG 2 cut(s) 45, 314
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 2 cut(s) 45, 314
FaeI CATG 1 cut(s) 49
FaiI YATR 6 cut(s) 47, 71, 101, 122, 306, 308
FaqI GGGAC 1 cut(s) 50
FatI CATG 1 cut(s) 45
FauI CCCGC 1 cut(s) 133
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 1 cut(s) 45
HaeIII GGCC 2 cut(s) 8, 272
HapII CCGG 1 cut(s) 158
Hin1II CATG 1 cut(s) 49
HinfI GANTC 2 cut(s) 23, 289
HpaII CCGG 1 cut(s) 158
Hpy188III TCNNGA 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4IV ACGT 1 cut(s) 87
HpyF3I CTNAG 1 cut(s) 258
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 1 cut(s) 49
LmnI GCTCC 1 cut(s) 108
LpnPI CCDG 7 cut(s) 72, 90, 171, 191, 262, 284, 289
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 83
MboII GAAGA 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 2 cut(s) 12, 150
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 3 cut(s) 74, 255, 262
MseI TTAA 1 cut(s) 54
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 2 cut(s) 159, 277
MssI GTTTAAAC 1 cut(s) 55
MvaI CCWGG 1 cut(s) 277
NciI CCSGG 1 cut(s) 159
NcoI CCATGG 1 cut(s) 45
NlaIII CATG 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 25
PfeI GAWTC 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 157
PleI GAGTC 1 cut(s) 17
PmeI GTTTAAAC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 275
PspGI CCWGG 1 cut(s) 275
PspXI VCTCGAGB 1 cut(s) 25
RsaI GTAC 2 cut(s) 90, 218
RsaNI GTAC 2 cut(s) 89, 217
SaqAI TTAA 1 cut(s) 54
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 159, 277
SduI GDGCHC 1 cut(s) 45
SetI ASST 7 cut(s) 61, 68, 85, 90, 232, 278, 320
SfcI CTRYAG 1 cut(s) 296
Sfr274I CTCGAG 1 cut(s) 25
SlaI CTCGAG 1 cut(s) 25
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 2 cut(s) 12, 150
SsiI CCGC 1 cut(s) 140
StyD4I CCNGG 2 cut(s) 157, 275
StyI CCWWGG 2 cut(s) 45, 314
TaaI ACNGT 1 cut(s) 132
TaiI ACGT 1 cut(s) 90
TaqI TCGA 2 cut(s) 19, 26
TasI AATT 2 cut(s) 12, 150
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TspDTI ATGAA 2 cut(s) 86, 299
XapI RAATTY 1 cut(s) 150
XhoI CTCGAG 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.