Rroxscaffold_1G00044760

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014824
Physical Location & Seq
Forward (+)
63772843 .. 63775815
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044760.1

Sequence Viewer

Length: 387 bp
ATGGGGCGGGATCCGAAGGTGTGGGCGGAGCCGATGGAGTTCAAGCCTGAGAGGTTTCTGAGTGATGAGGAGAACTTGTGTGATTTAACTGGTAGTAAGGAGATCAAGATGATGCCATTTGGGGTAGGGAGGAGGATGTGTCCTGGTTCGAATTTGGCAATGCTGCTTTTGGAGTACTTTGTGGCGAATTTGGTTTGGAAATTCGAGTGGAGAGCTGTGGAGGGAGTTGGGGTTGATTTGAGTGAGAAGGAGGAGACGAGTTGGGGGATGAATAATCCATTGCAGGCACATATCACTCCAAGCACAATTTTGCCCTCTCCTTCTTTGTCACTTAGAGAGAGAAGTCATCGGAGTCCTTGCCGGACTCCGGACTCCGGACTCCGGTAA

Protein Analysis

128

Amino Acids

14.55

Weight (kDa)

5.72

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 88 7.7e-22 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 367, 374
AciI CCGC 2 cut(s) 7, 26
AclWI GGATC 2 cut(s) 5, 18
AcsI RAATTY 3 cut(s) 151, 187, 200
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 3 cut(s) 367, 374, 381
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 2 cut(s) 5, 18
Aor13HI TCCGGA 2 cut(s) 367, 374
ApeKI GCWGC 1 cut(s) 163
ApoI RAATTY 3 cut(s) 151, 187, 200
AsuII TTCGAA 1 cut(s) 149
BamHI GGATCC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 150
BccI CCATC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 248
BisI GCNGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 165
BmcAI AGTACT 1 cut(s) 176
Bme1390I CCNGG 1 cut(s) 144
BmiI GGNNCC 2 cut(s) 12, 30
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 149
BsaWI WCCGGW 3 cut(s) 367, 374, 381
BsaXI ACNNNNNCTCC 6 cut(s) 62, 92, 124, 154, 216, 246
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 374, 381
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 2 cut(s) 165, 278
BseAI TCCGGA 2 cut(s) 367, 374
BseBI CCWGG 1 cut(s) 144
BseGI GGATG 2 cut(s) 141, 273
BseLI CCNNNNNNNGG 3 cut(s) 367, 374, 381
BseMI GCAATG 2 cut(s) 165, 278
BseMII CTCAG 2 cut(s) 39, 50
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 3 cut(s) 83, 145, 266
BseXI GCAGC 1 cut(s) 150
BsiSI CCGG 4 cut(s) 361, 368, 375, 382
BslI CCNNNNNNNGG 3 cut(s) 367, 374, 381
BsmAI GTCTC 1 cut(s) 248
BsmBI CGTCTC 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 149
Bsp13I TCCGGA 2 cut(s) 367, 374
Bsp143I GATC 2 cut(s) 10, 102
BspACI CCGC 2 cut(s) 7, 26
BspCNI CTCAG 2 cut(s) 40, 51
BspEI TCCGGA 2 cut(s) 367, 374
BspLI GGNNCC 2 cut(s) 12, 30
BspPI GGATC 2 cut(s) 5, 18
BspT104I TTCGAA 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 165, 278
BsrI ACTGG 1 cut(s) 94
BssMI GATC 2 cut(s) 10, 102
Bst2UI CCWGG 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 3 cut(s) 48, 59, 332
BstF5I GGATG 2 cut(s) 141, 273
BstKTI GATC 2 cut(s) 13, 105
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 2 cut(s) 10, 102
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstV1I GCAGC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BtsCI GGATG 2 cut(s) 141, 273
Cac8I GCNNGC 1 cut(s) 285
Csp6I GTAC 1 cut(s) 175
CviJI RGCY 3 cut(s) 31, 46, 215
CviKI_1 RGCY 3 cut(s) 31, 46, 215
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 3 cut(s) 48, 59, 332
DpnI GATC 2 cut(s) 12, 104
DpnII GATC 2 cut(s) 10, 102
EciI GGCGGA 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 142
Esp3I CGTCTC 1 cut(s) 248
FaiI YATR 1 cut(s) 291
Fnu4HI GCNGC 1 cut(s) 164
FokI GGATG 2 cut(s) 148, 280
Fsp4HI GCNGC 1 cut(s) 164
GluI GCNGC 1 cut(s) 164
HapII CCGG 4 cut(s) 361, 368, 375, 382
HinfI GANTC 4 cut(s) 352, 364, 371, 378
HpaII CCGG 4 cut(s) 361, 368, 375, 382
Hpy188I TCNGA 3 cut(s) 15, 60, 351
Hpy188III TCNNGA 3 cut(s) 106, 368, 375
HpyAV CCTTC 3 cut(s) 10, 241, 330
HpyCH4V TGCA 1 cut(s) 283
HpyF3I CTNAG 3 cut(s) 48, 59, 332
Kpn2I TCCGGA 2 cut(s) 367, 374
Kzo9I GATC 2 cut(s) 10, 102
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 7 cut(s) 60, 75, 129, 156, 269, 374, 381
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 327
MalI GATC 2 cut(s) 12, 104
MboI GATC 2 cut(s) 10, 102
MflI RGATCY 1 cut(s) 10
MluCI AATT 4 cut(s) 151, 187, 200, 306
MlyI GAGTC 4 cut(s) 358, 361, 365, 372
MnlI CCTC 7 cut(s) 45, 61, 123, 126, 214, 244, 325
MroI TCCGGA 2 cut(s) 367, 374
MseI TTAA 1 cut(s) 86
MspI CCGG 4 cut(s) 361, 368, 375, 382
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
NdeII GATC 2 cut(s) 10, 102
NlaIV GGNNCC 2 cut(s) 12, 30
NmuCI GTSAC 1 cut(s) 327
NspV TTCGAA 1 cut(s) 149
PkrI GCNGC 1 cut(s) 165
PleI GAGTC 4 cut(s) 358, 360, 365, 372
PpsI GAGTC 4 cut(s) 358, 360, 365, 372
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 12, 30
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 1 cut(s) 164
Sau3AI GATC 2 cut(s) 10, 102
ScaI AGTACT 1 cut(s) 176
SchI GAGTC 4 cut(s) 358, 361, 365, 372
ScrFI CCNGG 1 cut(s) 144
SetI ASST 3 cut(s) 21, 56, 217
SfaNI GCATC 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 149
Sse9I AATT 4 cut(s) 151, 187, 200, 306
SsiI CCGC 2 cut(s) 7, 26
StyD4I CCNGG 1 cut(s) 142
TaqI TCGA 2 cut(s) 149, 204
TasI AATT 4 cut(s) 151, 187, 200, 306
TatI WGTACW 1 cut(s) 174
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseFI GTSAC 1 cut(s) 327
TseI GCWGC 1 cut(s) 163
Tsp45I GTSAC 1 cut(s) 327
TspDTI ATGAA 1 cut(s) 284
XapI RAATTY 3 cut(s) 151, 187, 200
ZrmI AGTACT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.