Rh1DG412500

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014824
Physical Location & Seq
Forward (+)
62965976 .. 62968174
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG412500.1

Sequence Viewer

Length: 900 bp
ATGGTCCTATCCTCACCCTCTACATTGGATCCAGCCCTGCCATATTCGTTGCCAACCACTCCCTGGCAGTGGAACAATTTCTTAATGATCCGCAAAGAACTAGAAGATGTGTTCATTCCTCTTATACAAGAACGAAGCAAGGCAAAGCAGGAGAGGCTGAGCAAGGTGGACCAAAGTGACGAATTTGTGTTGTCGTATGTTGGTACATTGCAGGATCTCCAGCTCCCTGAAGAGAAGAGAAAGCTTGGGGAAGAAGAAATAGTTAGCCTTTGTTCCGAGATTCTCATGGCAGGAGTTGATACTACATCCACTGCATTGCTCTGGATCATGGCCAACATAGTCAAGTATCCACATGTACAAGAGAAGCTTGTCGCAGAGATCAAAGGGGTTGTGAACCATACAAAAGAAGAGGTGAAAGAGGAGGACTTGCAAAAGATGCCTTATCTGAAAGCTATGATCTTAGAGGGTCTAAGGCGTCACCCACCGGTCCACATTCTGTTGCCACACTCGGTCACACATGACATTGTTTTCGATGGTCATGTGGTGCCCAAGAATGCTACCGTCAATTTCACAGTGGCTGATATGGGGTGGGATCCAAAAGTGTGGGAAGACCCTATGGCTTTCAAGCCGGAGAGATTCTTGAGTGGTGGTGCAGGAGAAGGGTTTGATATAACGGGAAGTAGAGAGATCAAGATGATGCCTTTTGGGGCGGGAAGGAGAATTTGTCCTGCTTCTGGTTTAGCTATGCTTCATCTTGAGTATTTTGTGGCCAATCTGGTTTGGAGATTTGAATGGAAAACTGTGGATGGAGACAATGTGGATCTGTCGGAGACGATGGGGTTCACTGTCCTTATGAAGAATCCATTGCAGGTCAATATATCACAGAGAGTGAACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00140 ko00380 ko00830 ko00980 ko01100 ko04913 ko04917 ko04927 ko04934 ko05204 map00140 map00380 map00830 map00980 map01100 map04913 map04917 map04927 map04934 map05204
Pfam Domains
Protein Families
p450

Protein Analysis

299

Amino Acids

33.93

Weight (kDa)

5.45

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 13 - 288 1.3e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 859
AccB1I GGYRCC 1 cut(s) 544
AccB7I CCANNNNNTGG 1 cut(s) 63
AciI CCGC 2 cut(s) 91, 710
AclWI GGATC 8 cut(s) 23, 36, 82, 222, 332, 587, 600, 828
AcoI YGGCCR 2 cut(s) 330, 768
AcsI RAATTY 2 cut(s) 182, 720
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 475
AfaI GTAC 2 cut(s) 205, 357
AfiI CCNNNNNNNGG 3 cut(s) 63, 69, 734
AflIII ACRYGT 1 cut(s) 352
AgeI ACCGGT 1 cut(s) 484
AgsI TTSAA 2 cut(s) 625, 791
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 5 cut(s) 223, 244, 367, 452, 743
AluI AGCT 5 cut(s) 223, 244, 367, 452, 743
Alw26I GTCTC 2 cut(s) 804, 824
AlwI GGATC 8 cut(s) 23, 36, 82, 222, 332, 587, 600, 828
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 2 cut(s) 330, 768
ApoI RAATTY 2 cut(s) 182, 720
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
AsiGI ACCGGT 1 cut(s) 484
Asp700I GAANNNNTTC 1 cut(s) 77
AspS9I GGNCC 3 cut(s) 4, 169, 487
AsuHPI GGTGA 3 cut(s) 6, 424, 470
AvaII GGWCC 3 cut(s) 4, 169, 487
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 2 cut(s) 332, 770
BamHI GGATCC 2 cut(s) 28, 592
BanI GGYRCC 1 cut(s) 544
BbsI GAAGAC 1 cut(s) 615
BccI CCATC 3 cut(s) 527, 800, 829
BciT130I CCWGG 1 cut(s) 64
BciVI GTATCC 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 804, 824
BfaI CTAG 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 859
BfuI GTATCC 1 cut(s) 357
BlpI GCTNAGC 1 cut(s) 158
Bme1390I CCNGG 1 cut(s) 64
Bme18I GGWCC 3 cut(s) 4, 169, 487
BmgT120I GGNCC 3 cut(s) 4, 169, 487
BmiI GGNNCC 3 cut(s) 30, 546, 594
BmrFI CCNGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 426, 687
BpiI GAAGAC 1 cut(s) 615
BpmI CTGGAG 1 cut(s) 203
Bpu1102I GCTNAGC 1 cut(s) 158
BpuEI CTTGAG 2 cut(s) 661, 776
BsaHI GRCGYC 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 62
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 4 cut(s) 709, 739, 801, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 69, 734
Bse118I RCCGGY 1 cut(s) 484
Bse3DI GCAATG 3 cut(s) 206, 314, 863
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 62
BseGI GGATG 2 cut(s) 305, 811
BseLI CCNNNNNNNGG 3 cut(s) 63, 69, 734
BseMI GCAATG 3 cut(s) 206, 314, 863
BseMII CTCAG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 549
BsgI GTGCAG 1 cut(s) 672
BshFI GGCC 2 cut(s) 332, 770
BshNI GGYRCC 1 cut(s) 544
BshTI ACCGGT 1 cut(s) 484
BsiSI CCGG 2 cut(s) 485, 629
BslI CCNNNNNNNGG 3 cut(s) 63, 69, 734
BsmAI GTCTC 2 cut(s) 804, 824
BsmBI CGTCTC 1 cut(s) 824
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 2 cut(s) 332, 770
Bsp1286I GDGCHC 1 cut(s) 549
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
Bsp1720I GCTNAGC 1 cut(s) 158
BspACI CCGC 2 cut(s) 91, 710
BspANI GGCC 2 cut(s) 332, 770
BspCNI CTCAG 1 cut(s) 150
BspLI GGNNCC 3 cut(s) 30, 546, 594
BspMI ACCTGC 1 cut(s) 859
BspPI GGATC 8 cut(s) 23, 36, 82, 222, 332, 587, 600, 828
BspT107I GGYRCC 1 cut(s) 544
BsrDI GCAATG 3 cut(s) 206, 314, 863
BsrFI RCCGGY 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 355
BssAI RCCGGY 1 cut(s) 484
BssECI CCNNGG 1 cut(s) 62
BssMI GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
BssNI GRCGYC 1 cut(s) 475
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 4 cut(s) 562, 574, 802, 847
Bst6I CTCTTC 3 cut(s) 225, 230, 402
BstACI GRCGYC 1 cut(s) 475
BstAPI GCANNNNNTGC 1 cut(s) 436
BstAUI TGTACA 1 cut(s) 355
BstDEI CTNAG 3 cut(s) 158, 460, 470
BstF5I GGATG 2 cut(s) 305, 811
BstKTI GATC 9 cut(s) 31, 90, 217, 327, 381, 459, 595, 690, 823
BstMAI GTCTC 2 cut(s) 804, 824
BstMBI GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
BstMWI GCNNNNNNNGC 2 cut(s) 154, 436
BstNI CCWGG 1 cut(s) 64
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 549
BstV2I GAAGAC 1 cut(s) 615
BstX2I RGATCY 4 cut(s) 28, 214, 592, 820
BstXI CCANNNNNNTGG 1 cut(s) 603
BstYI RGATCY 4 cut(s) 28, 214, 592, 820
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 2 cut(s) 332, 770
BtsCI GGATG 2 cut(s) 305, 811
BtsI GCAGTG 2 cut(s) 74, 309
BtsIMutI CAGTG 4 cut(s) 74, 309, 579, 843
BveI ACCTGC 1 cut(s) 859
CaiI CAGNNNCTG 1 cut(s) 578
Cfr10I RCCGGY 1 cut(s) 484
Cfr13I GGNCC 3 cut(s) 4, 169, 487
CseI GACGC 1 cut(s) 464
Csp6I GTAC 2 cut(s) 204, 356
CspAI ACCGGT 1 cut(s) 484
CviAII CATG 5 cut(s) 286, 328, 353, 518, 539
CviQI GTAC 2 cut(s) 204, 356
DdeI CTNAG 3 cut(s) 158, 460, 470
DpnI GATC 9 cut(s) 30, 89, 216, 326, 380, 458, 594, 689, 822
DpnII GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
EaeI YGGCCR 2 cut(s) 330, 768
Eam1104I CTCTTC 3 cut(s) 225, 230, 402
EarI CTCTTC 3 cut(s) 225, 230, 402
Eco47I GGWCC 3 cut(s) 4, 169, 487
Eco57I CTGAAG 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 62
Esp3I CGTCTC 1 cut(s) 824
FaeI CATG 5 cut(s) 289, 331, 356, 521, 542
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 5 cut(s) 285, 327, 352, 517, 538
FauI CCCGC 1 cut(s) 703
FokI GGATG 2 cut(s) 292, 818
FspBI CTAG 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 2 cut(s) 332, 770
HapII CCGG 2 cut(s) 485, 629
HgaI GACGC 1 cut(s) 464
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 5 cut(s) 289, 331, 356, 521, 542
HindIII AAGCTT 2 cut(s) 242, 365
HinfI GANTC 3 cut(s) 280, 636, 859
HpaII CCGG 2 cut(s) 485, 629
HphI GGTGA 3 cut(s) 6, 424, 470
Hpy166II GTNNAC 5 cut(s) 169, 394, 490, 843, 892
Hpy188I TCNGA 3 cut(s) 277, 447, 829
Hpy188III TCNNGA 4 cut(s) 322, 640, 691, 755
Hpy8I GTNNAC 5 cut(s) 169, 394, 490, 843, 892
HpyAV CCTTC 2 cut(s) 653, 708
HpyCH4III ACNGT 4 cut(s) 562, 574, 802, 847
HpyCH4V TGCA 5 cut(s) 211, 314, 430, 653, 868
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 436
HpyF3I CTNAG 3 cut(s) 158, 460, 470
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 5 cut(s) 289, 331, 356, 521, 542
Kzo9I GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
LmnI GCTCC 1 cut(s) 228
LweI GCATC 2 cut(s) 426, 687
MaeI CTAG 1 cut(s) 101
MaeIII GTNAC 3 cut(s) 176, 476, 511
MalI GATC 9 cut(s) 30, 89, 216, 326, 380, 458, 594, 689, 822
MboI GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
MboII GAAGA 8 cut(s) 116, 242, 247, 263, 266, 419, 620, 868
MflI RGATCY 4 cut(s) 28, 214, 592, 820
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 2 cut(s) 332, 770
MluCI AATT 4 cut(s) 76, 182, 565, 720
MluNI TGGCCA 2 cut(s) 332, 770
MmeI TCCRAC 1 cut(s) 807
MnlI CCTC 8 cut(s) 22, 28, 129, 147, 403, 412, 415, 457
Mox20I TGGCCA 2 cut(s) 332, 770
MroXI GAANNNNTTC 1 cut(s) 77
MscI TGGCCA 2 cut(s) 332, 770
MseI TTAA 1 cut(s) 83
Msp20I TGGCCA 2 cut(s) 332, 770
MspI CCGG 2 cut(s) 485, 629
MspR9I CCNGG 1 cut(s) 64
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 2 cut(s) 154, 436
NdeII GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
NlaIII CATG 5 cut(s) 289, 331, 356, 521, 542
NlaIV GGNNCC 3 cut(s) 30, 546, 594
NmuCI GTSAC 3 cut(s) 176, 476, 511
NspI RCATGY 1 cut(s) 356
PciI ACATGT 1 cut(s) 352
PctI GAATGC 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 3 cut(s) 280, 636, 859
PflMI CCANNNNNTGG 1 cut(s) 63
PinAI ACCGGT 1 cut(s) 484
PscI ACATGT 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspN4I GGNNCC 3 cut(s) 30, 546, 594
PspPI GGNCC 3 cut(s) 4, 169, 487
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 4 cut(s) 28, 214, 592, 820
RsaI GTAC 2 cut(s) 205, 357
RsaNI GTAC 2 cut(s) 204, 356
SaqAI TTAA 1 cut(s) 83
Sau3AI GATC 9 cut(s) 28, 87, 214, 324, 378, 456, 592, 687, 820
Sau96I GGNCC 3 cut(s) 4, 169, 487
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 1 cut(s) 549
SetI ASST 8 cut(s) 168, 225, 246, 369, 414, 454, 745, 873
SfaNI GCATC 2 cut(s) 426, 687
SinI GGWCC 3 cut(s) 4, 169, 487
SmlI CTYRAG 2 cut(s) 640, 755
SmoI CTYRAG 2 cut(s) 640, 755
Sse9I AATT 4 cut(s) 76, 182, 565, 720
SsiI CCGC 2 cut(s) 91, 710
SspMI CTAG 1 cut(s) 101
StyD4I CCNGG 1 cut(s) 62
TaaI ACNGT 4 cut(s) 562, 574, 802, 847
TaqI TCGA 1 cut(s) 531
TaqII GACCGA 1 cut(s) 499
TasI AATT 4 cut(s) 76, 182, 565, 720
TatI WGTACW 1 cut(s) 355
TfiI GAWTC 3 cut(s) 280, 636, 859
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 4 cut(s) 74, 316, 579, 850
TseFI GTSAC 3 cut(s) 176, 476, 511
Tsp45I GTSAC 3 cut(s) 176, 476, 511
TspDTI ATGAA 3 cut(s) 103, 740, 869
TspRI CASTG 4 cut(s) 74, 316, 579, 850
Van91I CCANNNNNTGG 1 cut(s) 63
VpaK11BI GGWCC 3 cut(s) 4, 169, 487
XapI RAATTY 2 cut(s) 182, 720
XceI RCATGY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.