Rh5CG277000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014824
Physical Location & Seq
Reverse (-)
30133392 .. 30133856
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG277000.1

Sequence Viewer

Length: 465 bp
ATGAAGTACTTAAAAGCGGTGGTGTTGGAGGGTTTAAGGCGTCACCCCCCGGGGCACATTTTGATCCCACACTCAGTGATAGATGACGTCATTTTGGACGAACGTCATGCCATTCCCAAAGATTGCAGTATCAATTTCATGGTGGCGGAGATGGGGTGGGATCCAAAGGTATTGGCGGAGCCGATGGAGTTCAAGCCTGACAGGTTTTTGAGTGGTGAGGAGAATGTGTGTGATATAACAGGGAGTAAGGAGATTAAGATGATGCCGTTTGGGGCAGGGAGGAGGATGTGTCCCGGTTCAAGCTTGGCAATGCTGCTTTTGGAGTATTTTGTGGCGAATTTGGTTTGGAAGTTCGAGTGGAGAGCTGTTGAAGGAGTTGGGGTTGATTTGACTGATAAGATGGAGTCTAGTTGGGTGATGAAGAATCCTCTGCAGGCACATATCATTCTAAGGTTGAAGAAATGA

Protein Analysis

154

Amino Acids

17.55

Weight (kDa)

6.52

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 143 4.3e-33 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 90
AciI CCGC 3 cut(s) 17, 146, 176
AclWI GGATC 3 cut(s) 58, 155, 168
AcsI RAATTY 1 cut(s) 337
AcyI GRCGYC 2 cut(s) 40, 87
AdeI CACNNNGTG 1 cut(s) 76
AfaI GTAC 1 cut(s) 8
AgsI TTSAA 4 cut(s) 193, 300, 371, 457
AluBI AGCT 2 cut(s) 303, 365
AluI AGCT 2 cut(s) 303, 365
AlwI GGATC 3 cut(s) 58, 155, 168
Ama87I CYCGRG 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 1 cut(s) 337
AsuC2I CCSGG 3 cut(s) 50, 51, 294
AsuHPI GGTGA 3 cut(s) 35, 227, 427
AvaI CYCGRG 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 57
BamHI GGATCC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 300
BccI CCATC 3 cut(s) 145, 178, 394
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BcnI CCSGG 3 cut(s) 50, 51, 294
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 431
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 3 cut(s) 50, 51, 294
BmeT110I CYCGRG 1 cut(s) 49
BmiI GGNNCC 2 cut(s) 162, 180
BmrFI CCNGG 3 cut(s) 50, 51, 294
BmsI GCATC 1 cut(s) 252
BoxI GACNNNNGTC 1 cut(s) 102
BpuMI CCSGG 3 cut(s) 50, 51, 294
BsaHI GRCGYC 2 cut(s) 40, 87
BsaJI CCNNGG 3 cut(s) 48, 49, 50
BsaXI ACNNNNNCTCC 8 cut(s) 140, 170, 212, 242, 274, 304, 366, 396
Bse3DI GCAATG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 48, 49, 50
BseGI GGATG 1 cut(s) 291
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 2 cut(s) 233, 295
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 2 cut(s) 50, 294
BslFI GGGAC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 63, 160
BspACI CCGC 3 cut(s) 17, 146, 176
BspCNI CTCAG 1 cut(s) 86
BspLI GGNNCC 2 cut(s) 162, 180
BspMAI CTGCAG 1 cut(s) 435
BspPI GGATC 3 cut(s) 58, 155, 168
BsrDI GCAATG 1 cut(s) 315
BssECI CCNNGG 3 cut(s) 48, 49, 50
BssMI GATC 2 cut(s) 63, 160
BssNI GRCGYC 2 cut(s) 40, 87
BstACI GRCGYC 2 cut(s) 40, 87
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 2 cut(s) 73, 449
BstF5I GGATG 1 cut(s) 291
BstKTI GATC 2 cut(s) 66, 163
BstMBI GATC 2 cut(s) 63, 160
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 48, 49, 292
BstSFI CTRYAG 1 cut(s) 431
BstSLI GKGCMC 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
BtsCI GGATG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 435
Cfr9I CCCGGG 1 cut(s) 49
CseI GACGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 7
CviAII CATG 2 cut(s) 107, 139
CviJI RGCY 4 cut(s) 181, 196, 303, 365
CviKI_1 RGCY 4 cut(s) 181, 196, 303, 365
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 73, 449
DpnI GATC 2 cut(s) 65, 162
DpnII GATC 2 cut(s) 63, 160
DraIII CACNNNGTG 1 cut(s) 76
EciI GGCGGA 2 cut(s) 161, 191
Eco88I CYCGRG 1 cut(s) 49
FaeI CATG 2 cut(s) 110, 142
FaiI YATR 4 cut(s) 108, 140, 236, 441
FaqI GGGAC 1 cut(s) 276
FatI CATG 2 cut(s) 106, 138
Fnu4HI GCNGC 1 cut(s) 314
FokI GGATG 1 cut(s) 298
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 1 cut(s) 408
GluI GCNGC 1 cut(s) 314
HapII CCGG 2 cut(s) 50, 294
HgaI GACGC 1 cut(s) 29
Hin1I GRCGYC 2 cut(s) 40, 87
Hin1II CATG 2 cut(s) 110, 142
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 2 cut(s) 404, 424
HpaII CCGG 2 cut(s) 50, 294
HphI GGTGA 3 cut(s) 35, 227, 427
HpyAV CCTTC 1 cut(s) 365
HpyCH4IV ACGT 2 cut(s) 87, 103
HpyCH4V TGCA 2 cut(s) 126, 433
HpyF3I CTNAG 2 cut(s) 73, 449
HpySE526I ACGT 2 cut(s) 87, 103
Hsp92I GRCGYC 2 cut(s) 40, 87
Hsp92II CATG 2 cut(s) 110, 142
Kzo9I GATC 2 cut(s) 63, 160
LmnI GCTCC 1 cut(s) 178
LpnPI CCDG 7 cut(s) 63, 187, 210, 225, 261, 307, 419
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 87, 103
MaeIII GTNAC 1 cut(s) 41
MalI GATC 2 cut(s) 65, 162
MboI GATC 2 cut(s) 63, 160
MboII GAAGA 1 cut(s) 433
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 2 cut(s) 133, 337
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 5 cut(s) 22, 211, 273, 276, 438
MseI TTAA 3 cut(s) 11, 35, 255
MspI CCGG 2 cut(s) 50, 294
MspR9I CCNGG 3 cut(s) 50, 51, 294
NciI CCSGG 3 cut(s) 50, 51, 294
NdeII GATC 2 cut(s) 63, 160
NlaIII CATG 2 cut(s) 110, 142
NlaIV GGNNCC 2 cut(s) 162, 180
NmuCI GTSAC 1 cut(s) 41
PfeI GAWTC 1 cut(s) 424
PkrI GCNGC 1 cut(s) 315
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PshAI GACNNNNGTC 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 162, 180
PstI CTGCAG 1 cut(s) 435
PsuI RGATCY 1 cut(s) 160
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SaqAI TTAA 3 cut(s) 11, 35, 255
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 2 cut(s) 63, 160
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 3 cut(s) 50, 51, 294
SduI GDGCHC 1 cut(s) 57
SetI ASST 7 cut(s) 90, 106, 171, 206, 305, 367, 455
SfaNI GCATC 1 cut(s) 252
SfcI CTRYAG 1 cut(s) 431
SmaI CCCGGG 1 cut(s) 51
Sse9I AATT 2 cut(s) 133, 337
SsiI CCGC 3 cut(s) 17, 146, 176
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 3 cut(s) 48, 49, 292
TaiI ACGT 2 cut(s) 90, 106
TaqI TCGA 1 cut(s) 354
TasI AATT 2 cut(s) 133, 337
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 1 cut(s) 424
Tru1I TTAA 3 cut(s) 11, 35, 255
Tru9I TTAA 3 cut(s) 11, 35, 255
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 3 cut(s) 17, 127, 434
TspMI CCCGGG 1 cut(s) 49
TspRI CASTG 1 cut(s) 81
XapI RAATTY 1 cut(s) 337
XmaI CCCGGG 1 cut(s) 49
XspI CTAG 1 cut(s) 408
ZraI GACGTC 1 cut(s) 88
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.