Rmu_sc0000114.1_g000016

CDK5RAP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000114.1
OG0016943
Physical Location & Seq
Forward (+)
83226 .. 86149
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 834 bp
atggatcggaccaacgtgcataaacaaaaccaggacccccaaacactttatgaccatcagacagaacaccaccgccgtcgcacagcacaggacccaatgacccagaaccaccatcaccggatttatgtcccagaggtttccgtgtccgagatgtcttgggatattagtgttgaaactcctcaagttgatgcaattgatgatgtcaatttgccgaatcaatatgtttatgatgccattgagacagtgctatctgatgtttgcttggccatttctatgctgaaaaacgaggaaacgcgggactcgatcatgattctcgactccaaaagatttctagacagactaatgagttcattggaagaaaagaagcatcacgaagtgaagttgaaagagggtttgaagggcttagctaccaaacgcatggagctgcacaactcgttatcttcttcctggccaaagcaggaagcggctcttgcaaaaacccgcgagcttaagaagctgtgtgagagcaccctttcctccatgtttgatggaagacctgttcatataattgaagagattaatgcccgtttgaatagtggtgttgctgcatatgaaaatgtttgtctctggggtgaagcgtacaatatgagcgtgtactttgaacctcttcgtttttacttactaactttggggtgtcttcgggggttgatcatgcgaggaagcatgaatggcaccgtcagcacccagcagatttggatgagttgggttgagatggaaagtttcgagtttcgacgcggaaggcatggctcaccaaaatattggaagaaagaggttgatcatgcaattcatgagtga

Protein Analysis

277

Amino Acids

32.25

Weight (kDa)

6.17

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDK5RAP3 PF05600 72 - 183 3e-31 CDK5 regulatory subunit-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 710
AccII CGCG 3 cut(s) 295, 483, 774
AciI CCGC 5 cut(s) 73, 295, 464, 481, 774
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 264, 449
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 2 cut(s) 620, 635
AflII CTTAAG 1 cut(s) 488
AgsI TTSAA 6 cut(s) 173, 385, 397, 551, 571, 641
AjnI CCWGG 2 cut(s) 30, 446
AloI GAACNNNNNNTCC 2 cut(s) 522, 554
AluBI AGCT 4 cut(s) 407, 424, 487, 496
AluI AGCT 4 cut(s) 407, 424, 487, 496
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 2 cut(s) 233, 608
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 264, 449
ApeKI GCWGC 2 cut(s) 424, 584
AseI ATTAAT 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 645
AspS9I GGNCC 3 cut(s) 9, 34, 91
AsuHPI GGTGA 3 cut(s) 107, 623, 780
AvaII GGWCC 3 cut(s) 9, 34, 91
BalI TGGCCA 2 cut(s) 266, 451
BanI GGYRCC 1 cut(s) 710
BbsI GAAGAC 2 cut(s) 538, 668
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 2 cut(s) 411, 571
BccI CCATC 4 cut(s) 63, 120, 521, 745
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 2 cut(s) 32, 448
BclI TGATCA 2 cut(s) 687, 814
BcoDI GTCTC 2 cut(s) 233, 608
BfaI CTAG 1 cut(s) 332
BfrI CTTAAG 1 cut(s) 488
BisI GCNGC 3 cut(s) 425, 465, 585
BlpI GCTNAGC 1 cut(s) 403
BlsI GCNGC 3 cut(s) 426, 466, 586
Bme1390I CCNGG 2 cut(s) 32, 448
Bme18I GGWCC 3 cut(s) 9, 34, 91
BmgT120I GGNCC 3 cut(s) 9, 34, 91
BmiI GGNNCC 3 cut(s) 36, 93, 712
BmrFI CCNGG 2 cut(s) 32, 448
BmsI GCATC 3 cut(s) 178, 220, 376
BpiI GAAGAC 2 cut(s) 538, 668
Bpu1102I GCTNAGC 1 cut(s) 403
BpuEI CTTGAG 1 cut(s) 165
BsaWI WCCGGW 1 cut(s) 117
BseBI CCWGG 2 cut(s) 32, 448
BseGI GGATG 1 cut(s) 741
BseRI GAGGAG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 411, 571
BseYI CCCAGC 1 cut(s) 723
BsgI GTGCAG 1 cut(s) 410
Bsh1236I CGCG 3 cut(s) 295, 483, 774
BshFI GGCC 2 cut(s) 266, 451
BshNI GGYRCC 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 509
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 2 cut(s) 113, 311
BsmAI GTCTC 2 cut(s) 233, 608
BsmFI GGGAC 2 cut(s) 113, 311
BsnI GGCC 2 cut(s) 266, 451
Bsp1286I GDGCHC 1 cut(s) 509
Bsp143I GATC 4 cut(s) 4, 303, 687, 814
Bsp1720I GCTNAGC 1 cut(s) 403
BspACI CCGC 5 cut(s) 73, 295, 464, 481, 774
BspANI GGCC 2 cut(s) 266, 451
BspFNI CGCG 3 cut(s) 295, 483, 774
BspHI TCATGA 2 cut(s) 306, 826
BspLI GGNNCC 3 cut(s) 36, 93, 712
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 710
BspTI CTTAAG 1 cut(s) 488
BssMI GATC 4 cut(s) 4, 303, 687, 814
Bst2UI CCWGG 2 cut(s) 32, 448
Bst4CI ACNGT 2 cut(s) 244, 715
Bst6I CTCTTC 2 cut(s) 546, 651
BstAFI CTTAAG 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 485
BstDEI CTNAG 1 cut(s) 403
BstF5I GGATG 1 cut(s) 741
BstFNI CGCG 3 cut(s) 295, 483, 774
BstKTI GATC 4 cut(s) 7, 306, 690, 817
BstMAI GTCTC 2 cut(s) 233, 608
BstMBI GATC 4 cut(s) 4, 303, 687, 814
BstMWI GCNNNNNNNGC 4 cut(s) 470, 493, 708, 717
BstNI CCWGG 2 cut(s) 32, 448
BstSCI CCNGG 2 cut(s) 30, 446
BstUI CGCG 3 cut(s) 295, 483, 774
BstV1I GCAGC 2 cut(s) 411, 571
BstV2I GAAGAC 2 cut(s) 538, 668
BstXI CCANNNNNNTGG 2 cut(s) 418, 798
BsuRI GGCC 2 cut(s) 266, 451
BtsCI GGATG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 485
CciI TCATGA 2 cut(s) 306, 826
Cfr13I GGNCC 3 cut(s) 9, 34, 91
CseI GACGC 1 cut(s) 780
Csp6I GTAC 2 cut(s) 619, 634
CviAII CATG 8 cut(s) 307, 418, 520, 691, 703, 782, 818, 827
CviJI RGCY 9 cut(s) 266, 402, 407, 424, 451, 467, 487, 496, 786
CviKI_1 RGCY 9 cut(s) 266, 402, 407, 424, 451, 467, 487, 496, 786
CviQI GTAC 2 cut(s) 619, 634
DdeI CTNAG 1 cut(s) 403
DpnI GATC 4 cut(s) 6, 305, 689, 816
DpnII GATC 4 cut(s) 4, 303, 687, 814
DraIII CACNNNGTG 1 cut(s) 376
EaeI YGGCCR 2 cut(s) 264, 449
Eam1104I CTCTTC 2 cut(s) 546, 651
EarI CTCTTC 2 cut(s) 546, 651
Eco47I GGWCC 3 cut(s) 9, 34, 91
EcoO109I RGGNCCY 2 cut(s) 34, 91
EcoRII CCWGG 2 cut(s) 30, 446
FaeI CATG 8 cut(s) 310, 421, 523, 694, 706, 785, 821, 830
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FaqI GGGAC 2 cut(s) 113, 311
FatI CATG 8 cut(s) 306, 417, 519, 690, 702, 781, 817, 826
FauI CCCGC 2 cut(s) 288, 488
FauNDI CATATG 1 cut(s) 589
FbaI TGATCA 2 cut(s) 687, 814
Fnu4HI GCNGC 3 cut(s) 425, 465, 585
FokI GGATG 1 cut(s) 748
Fsp4HI GCNGC 3 cut(s) 425, 465, 585
FspBI CTAG 1 cut(s) 332
GluI GCNGC 3 cut(s) 425, 465, 585
GsaI CCCAGC 1 cut(s) 727
HaeIII GGCC 2 cut(s) 266, 451
HapII CCGG 1 cut(s) 118
HgaI GACGC 1 cut(s) 780
Hin1II CATG 8 cut(s) 310, 421, 523, 694, 706, 785, 821, 830
HinfI GANTC 4 cut(s) 214, 299, 310, 317
HpaII CCGG 1 cut(s) 118
HphI GGTGA 3 cut(s) 107, 623, 780
Hpy166II GTNNAC 1 cut(s) 634
Hpy188I TCNGA 4 cut(s) 9, 60, 148, 253
Hpy188III TCNNGA 5 cut(s) 307, 314, 332, 371, 827
Hpy8I GTNNAC 1 cut(s) 634
Hpy99I CGWCG 2 cut(s) 81, 774
HpyAV CCTTC 2 cut(s) 391, 771
HpyCH4III ACNGT 2 cut(s) 244, 715
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 6 cut(s) 19, 191, 427, 473, 587, 821
HpyF10VI GCNNNNNNNGC 4 cut(s) 470, 493, 708, 717
HpyF3I CTNAG 1 cut(s) 403
HpySE526I ACGT 1 cut(s) 15
Hsp92II CATG 8 cut(s) 310, 421, 523, 694, 706, 785, 821, 830
Ksp22I TGATCA 2 cut(s) 687, 814
Kzo9I GATC 4 cut(s) 4, 303, 687, 814
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 2 cut(s) 411, 571
LweI GCATC 3 cut(s) 178, 220, 376
MaeI CTAG 1 cut(s) 332
MaeII ACGT 1 cut(s) 15
MalI GATC 4 cut(s) 6, 305, 689, 816
MboI GATC 4 cut(s) 4, 303, 687, 814
MboII GAAGA 8 cut(s) 368, 432, 435, 543, 563, 638, 668, 814
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 509
MlsI TGGCCA 2 cut(s) 266, 451
MluCI AATT 4 cut(s) 192, 205, 546, 822
MluNI TGGCCA 2 cut(s) 266, 451
MlyI GAGTC 2 cut(s) 293, 311
MnlI CCTC 8 cut(s) 127, 189, 280, 382, 526, 654, 689, 802
Mox20I TGGCCA 2 cut(s) 266, 451
MroXI GAANNNNTTC 1 cut(s) 645
MscI TGGCCA 2 cut(s) 266, 451
MseI TTAA 2 cut(s) 489, 558
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 2 cut(s) 266, 451
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 2 cut(s) 32, 448
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 2 cut(s) 32, 448
MvnI CGCG 3 cut(s) 295, 483, 774
MwoI GCNNNNNNNGC 4 cut(s) 470, 493, 708, 717
NdeI CATATG 1 cut(s) 589
NdeII GATC 4 cut(s) 4, 303, 687, 814
NlaIII CATG 8 cut(s) 310, 421, 523, 694, 706, 785, 821, 830
NlaIV GGNNCC 3 cut(s) 36, 93, 712
PagI TCATGA 2 cut(s) 306, 826
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 2 cut(s) 214, 310
PkrI GCNGC 3 cut(s) 426, 466, 586
PleI GAGTC 2 cut(s) 293, 311
PpsI GAGTC 2 cut(s) 293, 311
PpuMI RGGWCCY 2 cut(s) 34, 91
PshBI ATTAAT 1 cut(s) 558
Psp5II RGGWCCY 2 cut(s) 34, 91
Psp6I CCWGG 2 cut(s) 30, 446
PspFI CCCAGC 1 cut(s) 723
PspGI CCWGG 2 cut(s) 30, 446
PspN4I GGNNCC 3 cut(s) 36, 93, 712
PspPI GGNCC 3 cut(s) 9, 34, 91
PspPPI RGGWCCY 2 cut(s) 34, 91
RsaI GTAC 2 cut(s) 620, 635
RsaNI GTAC 2 cut(s) 619, 634
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 2 cut(s) 489, 558
SatI GCNGC 3 cut(s) 425, 465, 585
Sau3AI GATC 4 cut(s) 4, 303, 687, 814
Sau96I GGNCC 3 cut(s) 9, 34, 91
SchI GAGTC 2 cut(s) 293, 311
ScrFI CCNGG 2 cut(s) 32, 448
SduI GDGCHC 1 cut(s) 509
SetI ASST 9 cut(s) 18, 138, 409, 426, 489, 498, 538, 646, 813
SfaNI GCATC 3 cut(s) 178, 220, 376
SinI GGWCC 3 cut(s) 9, 34, 91
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 180, 488
SmoI CTYRAG 2 cut(s) 180, 488
Sse9I AATT 4 cut(s) 192, 205, 546, 822
SsiI CCGC 5 cut(s) 73, 295, 464, 481, 774
SspI AATATT 1 cut(s) 797
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 30, 446
TaaI ACNGT 2 cut(s) 244, 715
TaiI ACGT 1 cut(s) 18
TaqI TCGA 4 cut(s) 302, 315, 762, 769
TasI AATT 4 cut(s) 192, 205, 546, 822
TatI WGTACW 1 cut(s) 633
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 2 cut(s) 214, 310
Tru1I TTAA 2 cut(s) 489, 558
Tru9I TTAA 2 cut(s) 489, 558
TscAI CASTG 1 cut(s) 249
TseI GCWGC 2 cut(s) 424, 584
TspDTI ATGAA 5 cut(s) 339, 530, 606, 719, 815
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 1 cut(s) 249
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 9, 34, 91
VspI ATTAAT 1 cut(s) 558
XbaI TCTAGA 1 cut(s) 331
XmnI GAANNNNTTC 1 cut(s) 645
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.