Rmu_sc0000280.1_g000015

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000280.1
OG0004561
Physical Location & Seq
Forward (+)
81516 .. 84639
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
atggctgacattacacaccctccaatggaccagctccaggaccttgagtactgtatagactccaaccctccttgggctaacaccatagttctggcatttcaaaactacattgtcatgcttggcaaaagtgtaatgatcccttcgttacttgtccctgcaatgggtggaagtgatggtgacaaagcacgagtaatacagactctcctttttgttgctggaattaagacacttctccaagcactttttggaaccaggctacctgctgtagttggaggctcctttgcttatgtcgttccaatagcctacattataaatgactccaaattgcatcggattactgatgatcatgaaagatttatacaaacaatgcgtgctatccaaggagctctaattgtagcttcaagcatacaaatcattctgggttacagccaagtttggggtctcttctcacggtttttcagtccacttggcatggcacctgtagttggattagtgggcttaggattctttgaacgaggatttccagagttgggaaagtatgtggaaattggaattccaatgctgttgttggtcatcgggttttctcaatatctgaagcatgtgagaccacttagagatgtccctatctttgaacggtttccagcattgatttgtgtcacaatcatttggatctattccctaatcttaactgccggtggggcataccgagacaaacccaaatcagctgtcgtacggatagagcaaatctcatatcgactgccccatggtatgcccaagaagaatgtgccttctcataatgtgtcttctggttcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

271

Amino Acids

29.88

Weight (kDa)

9.2

Isoelectric Point (pI)

30.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 27 - 230 1.1e-19 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 268
AccB1I GGYRCC 1 cut(s) 475
AclWI GGATC 2 cut(s) 130, 677
AcsI RAATTY 1 cut(s) 552
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 2 cut(s) 50, 732
AfiI CCNNNNNNNGG 8 cut(s) 25, 37, 73, 160, 161, 436, 485, 530
AgsI TTSAA 4 cut(s) 101, 402, 512, 632
AjnI CCWGG 2 cut(s) 36, 251
AluBI AGCT 4 cut(s) 34, 386, 398, 725
AluI AGCT 4 cut(s) 34, 386, 398, 725
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 3 cut(s) 446, 598, 702
AlwI GGATC 2 cut(s) 130, 677
ApoI RAATTY 1 cut(s) 552
ArsI GACNNNNNNTTYG 2 cut(s) 711, 743
Asp700I GAANNNNTTC 1 cut(s) 636
AspS9I GGNCC 2 cut(s) 28, 40
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 2 cut(s) 28, 40
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 388
BauI CACGAG 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 388
BccI CCATC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 38, 253
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 3 cut(s) 446, 598, 702
BfmI CTRYAG 2 cut(s) 264, 480
BfuAI ACCTGC 1 cut(s) 268
BglI GCCNNNNNGGC 1 cut(s) 698
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 2 cut(s) 38, 253
Bme18I GGWCC 2 cut(s) 28, 40
BmgT120I GGNCC 2 cut(s) 28, 40
BmiI GGNNCC 3 cut(s) 250, 277, 477
BmrFI CCNGG 2 cut(s) 38, 253
BmsI GCATC 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 795
BplI GAGNNNNNCTC 2 cut(s) 731, 763
BpmI CTGGAG 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 65
BsaI GGTCTC 2 cut(s) 446, 598
BsaJI CCNNGG 3 cut(s) 71, 379, 763
BsaXI ACNNNNNCTCC 3 cut(s) 34, 186, 216
Bsc4I CCNNNNNNNGG 8 cut(s) 25, 37, 73, 160, 161, 436, 485, 530
Bse118I RCCGGY 1 cut(s) 692
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 2 cut(s) 38, 253
BseDI CCNNGG 3 cut(s) 71, 379, 763
BseLI CCNNNNNNNGG 8 cut(s) 25, 37, 73, 160, 161, 436, 485, 530
BseMI GCAATG 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 1 cut(s) 693
BsiWI CGTACG 1 cut(s) 730
BslFI GGGAC 2 cut(s) 137, 605
BslI CCNNNNNNNGG 8 cut(s) 25, 37, 73, 160, 161, 436, 485, 530
BsmAI GTCTC 3 cut(s) 446, 598, 702
BsmFI GGGAC 2 cut(s) 137, 605
Bso31I GGTCTC 2 cut(s) 446, 598
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 3 cut(s) 135, 343, 669
Bsp19I CCATGG 1 cut(s) 763
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 250, 277, 477
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 2 cut(s) 130, 677
BspT107I GGYRCC 1 cut(s) 475
BspTNI GGTCTC 2 cut(s) 446, 598
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 692
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 3 cut(s) 71, 379, 763
BssMI GATC 3 cut(s) 135, 343, 669
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 71, 379, 763
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 38, 253
Bst4CI ACNGT 3 cut(s) 53, 453, 636
Bst6I CTCTTC 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 499, 611
BstDSI CCRYGG 1 cut(s) 763
BstKTI GATC 3 cut(s) 138, 346, 672
BstMAI GTCTC 3 cut(s) 446, 598, 702
BstMBI GATC 3 cut(s) 135, 343, 669
BstMWI GCNNNNNNNGC 1 cut(s) 698
BstNI CCWGG 2 cut(s) 38, 253
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 2 cut(s) 36, 251
BstSFI CTRYAG 2 cut(s) 264, 480
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 1 cut(s) 669
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 669
BtgI CCRYGG 1 cut(s) 763
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 372
CciI TCATGA 1 cut(s) 346
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 2 cut(s) 28, 40
Csp6I GTAC 2 cut(s) 49, 731
CviAII CATG 5 cut(s) 115, 347, 472, 599, 764
CviQI GTAC 2 cut(s) 49, 731
DdeI CTNAG 2 cut(s) 499, 611
DpnI GATC 3 cut(s) 137, 345, 671
DpnII GATC 3 cut(s) 135, 343, 669
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 3 cut(s) 71, 379, 763
Eco24I GRGCYC 1 cut(s) 388
Eco31I GGTCTC 2 cut(s) 446, 598
Eco47I GGWCC 2 cut(s) 28, 40
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 386
EcoO109I RGGNCCY 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 552
EcoRII CCWGG 2 cut(s) 36, 251
EcoT14I CCWWGG 3 cut(s) 71, 379, 763
EcoT38I GRGCYC 1 cut(s) 388
ErhI CCWWGG 3 cut(s) 71, 379, 763
FaeI CATG 5 cut(s) 118, 350, 475, 602, 767
FaqI GGGAC 2 cut(s) 137, 605
FatI CATG 5 cut(s) 114, 346, 471, 598, 763
FbaI TGATCA 1 cut(s) 343
FriOI GRGCYC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 20
HapII CCGG 1 cut(s) 693
Hin1II CATG 5 cut(s) 118, 350, 475, 602, 767
HinfI GANTC 4 cut(s) 59, 199, 317, 504
HpaII CCGG 1 cut(s) 693
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 2 cut(s) 333, 594
Hpy188III TCNNGA 2 cut(s) 347, 524
Hpy8I GTNNAC 1 cut(s) 464
HpyAV CCTTC 2 cut(s) 150, 798
HpyCH4III ACNGT 3 cut(s) 53, 453, 636
HpyCH4V TGCA 2 cut(s) 158, 328
HpyF10VI GCNNNNNNNGC 1 cut(s) 698
HpyF3I CTNAG 2 cut(s) 499, 611
Hsp92II CATG 5 cut(s) 118, 350, 475, 602, 767
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 3 cut(s) 135, 343, 669
LmnI GCTCC 3 cut(s) 39, 281, 383
LweI GCATC 1 cut(s) 337
MaeIII GTNAC 4 cut(s) 144, 176, 422, 655
MalI GATC 3 cut(s) 137, 345, 671
MboI GATC 3 cut(s) 135, 343, 669
MboII GAAGA 3 cut(s) 436, 790, 795
MflI RGATCY 1 cut(s) 669
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 5 cut(s) 219, 323, 390, 546, 552
MlyI GAGTC 3 cut(s) 53, 193, 311
MmeI TCCRAC 3 cut(s) 87, 250, 466
MnlI CCTC 4 cut(s) 30, 78, 266, 509
MroXI GAANNNNTTC 1 cut(s) 636
MseI TTAA 3 cut(s) 222, 686, 814
MslI CAYNNNNRTG 1 cut(s) 113
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 1 cut(s) 693
MspR9I CCNGG 2 cut(s) 38, 253
MvaI CCWGG 2 cut(s) 38, 253
MwoI GCNNNNNNNGC 1 cut(s) 698
NcoI CCATGG 1 cut(s) 763
NdeII GATC 3 cut(s) 135, 343, 669
NlaIII CATG 5 cut(s) 118, 350, 475, 602, 767
NlaIV GGNNCC 3 cut(s) 250, 277, 477
NmuCI GTSAC 2 cut(s) 176, 655
NspI RCATGY 1 cut(s) 602
PagI TCATGA 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 1 cut(s) 504
Pfl23II CGTACG 1 cut(s) 730
PfoI TCCNGGA 1 cut(s) 36
PleI GAGTC 3 cut(s) 53, 193, 311
PpsI GAGTC 3 cut(s) 53, 193, 311
PpuMI RGGWCCY 1 cut(s) 40
PsiI TTATAA 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 36, 251
PspGI CCWGG 2 cut(s) 36, 251
PspLI CGTACG 1 cut(s) 730
PspN4I GGNNCC 3 cut(s) 250, 277, 477
PspPI GGNCC 2 cut(s) 28, 40
PspPPI RGGWCCY 1 cut(s) 40
PsuI RGATCY 1 cut(s) 669
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 2 cut(s) 50, 732
RsaNI GTAC 2 cut(s) 49, 731
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 3 cut(s) 222, 686, 814
Sau3AI GATC 3 cut(s) 135, 343, 669
Sau96I GGNCC 2 cut(s) 28, 40
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 3 cut(s) 53, 193, 311
ScrFI CCNGG 2 cut(s) 38, 253
SduI GDGCHC 1 cut(s) 388
SetI ASST 7 cut(s) 36, 45, 262, 388, 400, 481, 727
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 2 cut(s) 264, 480
SinI GGWCC 2 cut(s) 28, 40
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 5 cut(s) 219, 323, 390, 546, 552
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 2 cut(s) 36, 251
StyI CCWWGG 3 cut(s) 71, 379, 763
TaaI ACNGT 3 cut(s) 53, 453, 636
TaqI TCGA 1 cut(s) 754
TasI AATT 5 cut(s) 219, 323, 390, 546, 552
TatI WGTACW 1 cut(s) 48
TfiI GAWTC 1 cut(s) 504
Tru1I TTAA 3 cut(s) 222, 686, 814
Tru9I TTAA 3 cut(s) 222, 686, 814
TseFI GTSAC 2 cut(s) 176, 655
Tsp45I GTSAC 2 cut(s) 176, 655
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 1 cut(s) 748
VpaK11BI GGWCC 2 cut(s) 28, 40
XapI RAATTY 1 cut(s) 552
XceI RCATGY 1 cut(s) 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 636
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.