Rmu_sc0000594.1_g000072

GDSL esterase lipase At4g26790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000594.1
OG0011664
Physical Location & Seq
Forward (+)
277409 .. 278615
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 879 bp
atggcatttaaagcaatttattggtttctcctaacccacttcctcctgcaaatttctagggtgtatgcaagagttccggcaatcattgtgtttggagactcttcggttgatgcaggaaacaataaccaggtatcaacaatcctaaagagtgactttccaccctacggtcgtgatttctatggtggacagcctacagggagattttccaatggtaggattccaccagacttcatttctgaggcttttgggatcaatccattgatacctgcatttttggatccattttataacattaaggattttgctactggagtttgctttgcctccgcaggaactggttatgataatgccacttctgatgtcctatctgtgatacctctttggaaggaattggagtactacaaagaataccagcaggacctcagaatctatctaggaaatgatgaagcaaacgagattctgggagaagcactttacttaatgagtataggaactaatgatttcctagaaaactactatctattatcaaacagatcatcggagtattccacagaggagtaccagaattttctagcaaaaattgctggaaattttatcacagagctcttccagcttggagctcgaaagatatctctaggcggccttcctccaatgggttgtctgccattggagagaactagaaatcttcttttggggagtgattgtgttgagacgtacaatgatgtggctaggagtttcaataacaagttggaggaattagttgacagactgaacggggaactagttggaatccagcttgtgcttgcaaacccatactatattctctcggatatcatacaaaaccctgaatcctttggtgagttttttttcatgttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

292

Amino Acids

32.95

Weight (kDa)

4.46

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 260 2.1e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26790 AT4G26790
fragaria_vesca FvH4_3g23490 FvH4_3g23490 FvH4_3g23490
malus_domestica MD11G1210200.v1.1
prunus_persica Prupe.4G222900_v2.0.a1
pyrus_communis pycom11g18580
rosa_chinensis RchiOBHm_Chr5g0041341
rosa_laevigata RLG00000034054
rosa_multiflora Rmu_sc0000594.1_g000072
rosa_roxburghii Rroxscaffold_1G00039290
rosa_rugosa Rorug05G0191600 Rorug05G0191700
rosa_samantha Rh5AG277700 Rh5BG282400 Rh5CG314800 Rh5DG291200
rosa_wichuraiana Rw5G026140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 274
AciI CCGC 2 cut(s) 327, 639
AclWI GGATC 3 cut(s) 257, 272, 285
AcsI RAATTY 3 cut(s) 51, 565, 589
AfaI GTAC 3 cut(s) 398, 560, 716
AfiI CCNNNNNNNGG 3 cut(s) 164, 213, 653
AgsI TTSAA 1 cut(s) 739
AhlI ACTAGT 1 cut(s) 781
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 4 cut(s) 604, 613, 620, 796
AluI AGCT 4 cut(s) 604, 613, 620, 796
Alw21I GWGCWC 2 cut(s) 606, 622
Alw26I GTCTC 2 cut(s) 90, 704
AlwI GGATC 3 cut(s) 257, 272, 285
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 1 cut(s) 640
ApoI RAATTY 3 cut(s) 51, 565, 589
AspS9I GGNCC 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 869
AvaII GGWCC 1 cut(s) 418
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 606, 622
Bbv12I GWGCWC 2 cut(s) 606, 622
BciT130I CCWGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 90, 704
BcuI ACTAGT 1 cut(s) 781
BfaI CTAG 8 cut(s) 57, 434, 506, 572, 635, 678, 729, 782
BfmI CTRYAG 1 cut(s) 192
BfuAI ACCTGC 1 cut(s) 274
BisI GCNGC 1 cut(s) 640
BlsI GCNGC 1 cut(s) 641
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 688, 718
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 213, 653
Bse1I ACTGG 2 cut(s) 313, 340
BseBI CCWGG 1 cut(s) 128
BseLI CCNNNNNNNGG 3 cut(s) 164, 213, 653
BseMII CTCAG 2 cut(s) 228, 436
BseNI ACTGG 2 cut(s) 313, 340
BseRI GAGGAG 1 cut(s) 569
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 1 cut(s) 642
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 2 cut(s) 606, 622
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 164, 213, 653
BsmAI GTCTC 2 cut(s) 90, 704
BsmBI CGTCTC 1 cut(s) 704
BsnI GGCC 1 cut(s) 642
Bsp1286I GDGCHC 2 cut(s) 606, 622
Bsp143I GATC 3 cut(s) 249, 277, 533
BspACI CCGC 2 cut(s) 327, 639
BspANI GGCC 1 cut(s) 642
BspCNI CTCAG 2 cut(s) 229, 435
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 257, 272, 285
BspQI GCTCTTC 1 cut(s) 611
BsrI ACTGG 2 cut(s) 313, 340
BssMI GATC 3 cut(s) 249, 277, 533
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 167
Bst6I CTCTTC 2 cut(s) 106, 611
BstAPI GCANNNNNTGC 1 cut(s) 581
BstC8I GCNNGC 1 cut(s) 804
BstDEI CTNAG 2 cut(s) 237, 422
BstKTI GATC 3 cut(s) 252, 280, 536
BstMAI GTCTC 2 cut(s) 90, 704
BstMBI GATC 3 cut(s) 249, 277, 533
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 3 cut(s) 11, 581, 610
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 1 cut(s) 642
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 804
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 418
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 3 cut(s) 397, 559, 715
CviAII CATG 1 cut(s) 871
CviJI RGCY 8 cut(s) 190, 242, 604, 613, 620, 642, 728, 796
CviKI_1 RGCY 8 cut(s) 190, 242, 604, 613, 620, 642, 728, 796
CviQI GTAC 3 cut(s) 397, 559, 715
DdeI CTNAG 2 cut(s) 237, 422
DpnI GATC 3 cut(s) 251, 279, 535
DpnII GATC 3 cut(s) 249, 277, 533
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 106, 611
EarI CTCTTC 2 cut(s) 106, 611
Ecl136II GAGCTC 2 cut(s) 604, 620
Eco24I GRGCYC 2 cut(s) 606, 622
Eco32I GATATC 2 cut(s) 630, 832
Eco47I GGWCC 1 cut(s) 418
Eco53kI GAGCTC 2 cut(s) 604, 620
EcoICRI GAGCTC 2 cut(s) 604, 620
EcoO109I RGGNCCY 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 126
EcoRV GATATC 2 cut(s) 630, 832
EcoT38I GRGCYC 2 cut(s) 606, 622
Esp3I CGTCTC 1 cut(s) 704
FaeI CATG 1 cut(s) 874
FaiI YATR 9 cut(s) 66, 180, 288, 342, 488, 814, 819, 836, 872
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 1 cut(s) 870
Fnu4HI GCNGC 1 cut(s) 640
FriOI GRGCYC 2 cut(s) 606, 622
Fsp4HI GCNGC 1 cut(s) 640
FspBI CTAG 8 cut(s) 57, 434, 506, 572, 635, 678, 729, 782
GluI GCNGC 1 cut(s) 640
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 1 cut(s) 642
HapII CCGG 1 cut(s) 77
Hin1II CATG 1 cut(s) 874
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HinfI GANTC 6 cut(s) 98, 217, 426, 457, 789, 848
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 869
Hpy166II GTNNAC 2 cut(s) 185, 763
Hpy188I TCNGA 5 cut(s) 238, 358, 425, 541, 829
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 2 cut(s) 185, 763
HpyAV CCTTC 2 cut(s) 379, 653
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 713
HpyCH4V TGCA 5 cut(s) 49, 68, 113, 269, 806
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 581, 610
HpyF3I CTNAG 2 cut(s) 237, 422
HpySE526I ACGT 1 cut(s) 713
Hsp92II CATG 1 cut(s) 874
Kzo9I GATC 3 cut(s) 249, 277, 533
LguI GCTCTTC 1 cut(s) 611
LmnI GCTCC 1 cut(s) 617
LweI GCATC 1 cut(s) 100
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 8 cut(s) 57, 434, 506, 572, 635, 678, 729, 782
MaeII ACGT 1 cut(s) 713
MaeIII GTNAC 1 cut(s) 149
MalI GATC 3 cut(s) 251, 279, 535
MboI GATC 3 cut(s) 249, 277, 533
MboII GAAGA 3 cut(s) 93, 598, 677
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 606, 622
MluCI AATT 7 cut(s) 15, 51, 389, 565, 579, 589, 755
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 729, 766
MnlI CCTC 8 cut(s) 53, 232, 334, 387, 431, 547, 657, 745
MseI TTAA 3 cut(s) 9, 294, 479
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 3 cut(s) 11, 581, 610
NdeII GATC 3 cut(s) 249, 277, 533
NlaIII CATG 1 cut(s) 874
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 611
PfeI GAWTC 5 cut(s) 217, 426, 457, 789, 848
PkrI GCNGC 1 cut(s) 641
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 418
PsiI TTATAA 1 cut(s) 288
Psp124BI GAGCTC 2 cut(s) 606, 622
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 418
PspPPI RGGWCCY 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 3 cut(s) 398, 560, 716
RsaNI GTAC 3 cut(s) 397, 559, 715
SacI GAGCTC 2 cut(s) 606, 622
SapI GCTCTTC 1 cut(s) 611
SaqAI TTAA 3 cut(s) 9, 294, 479
SatI GCNGC 1 cut(s) 640
Sau3AI GATC 3 cut(s) 249, 277, 533
Sau96I GGNCC 1 cut(s) 418
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 2 cut(s) 606, 622
SetI ASST 9 cut(s) 132, 268, 379, 423, 606, 615, 622, 716, 798
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 1 cut(s) 100
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 418
SpeI ACTAGT 1 cut(s) 781
Sse9I AATT 7 cut(s) 15, 51, 389, 565, 579, 589, 755
SsiI CCGC 2 cut(s) 327, 639
SspMI CTAG 8 cut(s) 57, 434, 506, 572, 635, 678, 729, 782
SstI GAGCTC 2 cut(s) 606, 622
StyD4I CCNGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 1 cut(s) 716
TaqI TCGA 1 cut(s) 622
TasI AATT 7 cut(s) 15, 51, 389, 565, 579, 589, 755
TatI WGTACW 1 cut(s) 396
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 5 cut(s) 217, 426, 457, 789, 848
Tru1I TTAA 3 cut(s) 9, 294, 479
Tru9I TTAA 3 cut(s) 9, 294, 479
TseFI GTSAC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 3 cut(s) 220, 459, 859
VpaK11BI GGWCC 1 cut(s) 418
XapI RAATTY 3 cut(s) 51, 565, 589
XspI CTAG 8 cut(s) 57, 434, 506, 572, 635, 678, 729, 782
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.