Rmu_sc0000680.1_g000045

Belongs to the RuvB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000680.1
OG0016455
Physical Location & Seq
Forward (+)
174606 .. 181456
6851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000680.1_g000045.1.cds

Sequence Viewer

Length: 1062 bp
atgaggatgatcagggtgaggagaagatcacgaaaatgggaaggatcggccgagttgatgccggaaaactgtttttccggccgggtcaccgatcggaggctgggatgccttcgatccaaggctggaggcagtgccggggcaaggcgacaccggagggaggctcggcatagggcggtgattccaggataccccgggtccggggccggaggaggccgtaggagccgttttcggccgggtcgggccgacggaaacccgccggattggtgtgagtcacacaacttgttgttgggctgttgtatcctggaggggacaagtcaagagatttctggtgcaccggcattgttccgcaaatgcccacgacgagaatcctcgccgttgcttgctcggacagcaaacggagaagcccgctgccgccgccctcttataaaccctaaggtttctagattatggatgttatgtttgcagaatgatcgtcttatccgcataggttccattccattctgcccaatgcatgggtctgaaggatattcctcagaagttgagataacagaggttgctgttggtgatgttataaacattgaagcaaacagtggggcagtaaaaagggtaggcagaattgatgcattgactgcggaattcgatcttgaagcagaagagtatgttccacttccaaaaggagaggttcacaaagaggagattgtgcaggatgtaactctgcacgatctagatgcgaatgcacaacctcaaggtgggctagacatattatctctaatgggtccaatgatgaagccaaggaaaacagaaatgacagacaaattgctacaagaaataaacaaggtacaagcttgtgattgtgtggggagcgggaaccaaagtcaatgccttctatgtggtagcttaagatgcgctgcccagaaaacctgcagttgcaggtcaggttgctgcccagaacctacaactcggacctcctccgacctcgaacgctgcccagacccgtccgccaagccccgagctgccgtcgcctcctggaacgccgctgctgcttcgccgtccttactttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000491 GO:0000492 GO:0000785 GO:0000790 GO:0000812 GO:0002682 GO:0002831 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005829 GO:0006325 GO:0006338 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009888 GO:0009889 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010604 GO:0010628 GO:0010755 GO:0010756 GO:0010941 GO:0010954 GO:0016043 GO:0016363 GO:0016462 GO:0016569 GO:0016570 GO:0016573 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0030162 GO:0031011 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032268 GO:0032270 GO:0032392 GO:0032502 GO:0032508 GO:0032991 GO:0033202 GO:0034399 GO:0034622 GO:0034708 GO:0035097 GO:0035267 GO:0036211 GO:0042623 GO:0042981 GO:0043067 GO:0043138 GO:0043140 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043900 GO:0043933 GO:0043967 GO:0043968 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044665 GO:0045088 GO:0045862 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048856 GO:0050776 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070035 GO:0070603 GO:0070613 GO:0071103 GO:0071339 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080134 GO:0097255 GO:0097346 GO:0140097 GO:1900150 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1903317 GO:1903319 GO:1903506 GO:1904949 GO:1990234 GO:2000072 GO:2000112 GO:2000269 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
TIP49

Protein Analysis

353

Amino Acids

38.46

Weight (kDa)

9.0

Isoelectric Point (pI)

66.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 425, 572
Acc36I ACCTGC 2 cut(s) 921, 929
AccB7I CCANNNNNTGG 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 864
AclWI GGATC 2 cut(s) 52, 108
AcoI YGGCCR 3 cut(s) 48, 79, 230
AcsI RAATTY 1 cut(s) 635
AcuI CTGAAG 1 cut(s) 540
AfaI GTAC 1 cut(s) 840
AfiI CCNNNNNNNGG 7 cut(s) 96, 141, 157, 197, 198, 512, 749
AflII CTTAAG 1 cut(s) 898
AgsI TTSAA 2 cut(s) 581, 647
AjnI CCWGG 3 cut(s) 181, 300, 1025
AluBI AGCT 3 cut(s) 845, 897, 1013
AluI AGCT 3 cut(s) 845, 897, 1013
Alw21I GWGCWC 1 cut(s) 334
Alw44I GTGCAC 1 cut(s) 330
AlwI GGATC 2 cut(s) 52, 108
Ama87I CYCGRG 2 cut(s) 191, 1008
AoxI GGCC 6 cut(s) 48, 79, 201, 211, 230, 240
ApaLI GTGCAC 1 cut(s) 330
ApeKI GCWGC 7 cut(s) 408, 908, 942, 984, 1013, 1037, 1040
ApoI RAATTY 1 cut(s) 635
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 5 cut(s) 195, 201, 240, 776, 963
AsuC2I CCSGG 6 cut(s) 83, 136, 192, 193, 199, 234
AsuHPI GGTGA 4 cut(s) 28, 79, 187, 575
AvaI CYCGRG 2 cut(s) 191, 1008
AvaII GGWCC 3 cut(s) 195, 776, 963
AxyI CCTNAGG 1 cut(s) 432
BaeGI GKGCMC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 7 cut(s) 395, 895, 929, 971, 1000, 1024, 1027
BceAI ACGGC 5 cut(s) 198, 207, 358, 1001, 1033
BcgI CGANNNNNNTGC 4 cut(s) 452, 486, 710, 744
BciT130I CCWGG 3 cut(s) 183, 302, 1027
BciVI GTATCC 2 cut(s) 179, 308
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 6 cut(s) 83, 136, 192, 193, 199, 234
BfaI CTAG 3 cut(s) 441, 725, 755
BfmI CTRYAG 1 cut(s) 922
BfrI CTTAAG 1 cut(s) 898
BfuAI ACCTGC 2 cut(s) 921, 929
BfuI GTATCC 2 cut(s) 179, 308
Bme1390I CCNGG 9 cut(s) 83, 136, 183, 192, 193, 199, 234, 302, 1027
Bme18I GGWCC 3 cut(s) 195, 776, 963
BmeT110I CYCGRG 2 cut(s) 191, 1008
BmgT120I GGNCC 5 cut(s) 195, 201, 240, 776, 963
BmiI GGNNCC 6 cut(s) 196, 202, 221, 490, 777, 869
BmrFI CCNGG 9 cut(s) 83, 136, 183, 192, 193, 199, 234, 302, 1027
BmsI GCATC 5 cut(s) 48, 95, 610, 718, 893
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpmI CTGGAG 2 cut(s) 144, 323
BpuEI CTTGAG 1 cut(s) 729
BpuMI CCSGG 6 cut(s) 83, 136, 192, 193, 199, 234
BsaJI CCNNGG 6 cut(s) 117, 135, 190, 191, 198, 791
BsaWI WCCGGW 1 cut(s) 150
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 141, 157, 197, 198, 512, 749
Bse118I RCCGGY 1 cut(s) 334
Bse21I CCTNAGG 1 cut(s) 432
BseBI CCWGG 3 cut(s) 183, 302, 1027
BseDI CCNNGG 6 cut(s) 117, 135, 190, 191, 198, 791
BseGI GGATG 4 cut(s) 12, 110, 456, 712
BseLI CCNNNNNNNGG 7 cut(s) 96, 141, 157, 197, 198, 512, 749
BseMII CTCAG 1 cut(s) 546
BseRI GAGGAG 4 cut(s) 34, 222, 707, 958
BseSI GKGCMC 1 cut(s) 334
BseX3I CGGCCG 3 cut(s) 48, 79, 230
BseXI GCAGC 7 cut(s) 395, 895, 929, 971, 1000, 1024, 1027
BseYI CCCAGC 1 cut(s) 100
BsgI GTGCAG 2 cut(s) 701, 722
Bsh1285I CGRYCG 4 cut(s) 51, 82, 94, 233
BshFI GGCC 6 cut(s) 50, 81, 203, 213, 232, 242
BsiEI CGRYCG 4 cut(s) 51, 82, 94, 233
BsiHKAI GWGCWC 1 cut(s) 334
BsiHKCI CYCGRG 2 cut(s) 191, 1008
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 7 cut(s) 96, 141, 157, 197, 198, 512, 749
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 6 cut(s) 50, 81, 203, 213, 232, 242
BsoBI CYCGRG 2 cut(s) 191, 1008
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
BspANI GGCC 6 cut(s) 50, 81, 203, 213, 232, 242
BspCNI CTCAG 1 cut(s) 545
BspLI GGNNCC 6 cut(s) 196, 202, 221, 490, 777, 869
BspMAI CTGCAG 1 cut(s) 926
BspMI ACCTGC 2 cut(s) 921, 929
BspPI GGATC 2 cut(s) 52, 108
BspTI CTTAAG 1 cut(s) 898
BsrBI CCGCTC 1 cut(s) 864
BsrFI RCCGGY 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 6 cut(s) 117, 135, 190, 191, 198, 791
BssMI GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
BssT1I CCWWGG 2 cut(s) 117, 791
Bst2UI CCWGG 3 cut(s) 183, 302, 1027
Bst4CI ACNGT 2 cut(s) 71, 590
Bst6I CTCTTC 1 cut(s) 648
BstAFI CTTAAG 1 cut(s) 898
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 381, 406
BstDEI CTNAG 2 cut(s) 432, 532
BstEII GGTNACC 1 cut(s) 85
BstF5I GGATG 4 cut(s) 12, 110, 456, 712
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 8 cut(s) 12, 29, 47, 94, 116, 472, 643, 724
BstMBI GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
BstMCI CGRYCG 4 cut(s) 51, 82, 94, 233
BstMWI GCNNNNNNNGC 7 cut(s) 219, 389, 414, 629, 903, 1019, 1040
BstNI CCWGG 3 cut(s) 183, 302, 1027
BstPI GGTNACC 1 cut(s) 85
BstSCI CCNGG 9 cut(s) 81, 134, 181, 190, 191, 197, 232, 300, 1025
BstSFI CTRYAG 1 cut(s) 922
BstSLI GKGCMC 1 cut(s) 334
BstV1I GCAGC 7 cut(s) 395, 895, 929, 971, 1000, 1024, 1027
BstZI CGGCCG 3 cut(s) 48, 79, 230
Bsu36I CCTNAGG 1 cut(s) 432
BsuI GTATCC 2 cut(s) 179, 308
BsuRI GGCC 6 cut(s) 50, 81, 203, 213, 232, 242
BtsCI GGATG 4 cut(s) 12, 110, 456, 712
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 595
BveI ACCTGC 2 cut(s) 921, 929
Cac8I GCNNGC 2 cut(s) 381, 406
CfoI GCGC 1 cut(s) 908
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 5 cut(s) 195, 201, 240, 776, 963
Cfr9I CCCGGG 1 cut(s) 191
Csp6I GTAC 1 cut(s) 839
CviAII CATG 1 cut(s) 512
CviQI GTAC 1 cut(s) 839
DdeI CTNAG 2 cut(s) 432, 532
DpnI GATC 8 cut(s) 11, 28, 46, 93, 115, 471, 642, 723
DpnII GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
EaeI YGGCCR 3 cut(s) 48, 79, 230
EagI CGGCCG 3 cut(s) 48, 79, 230
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
EciI GGCGGA 1 cut(s) 988
EclXI CGGCCG 3 cut(s) 48, 79, 230
Eco130I CCWWGG 2 cut(s) 117, 791
Eco47I GGWCC 3 cut(s) 195, 776, 963
Eco52I CGGCCG 3 cut(s) 48, 79, 230
Eco57I CTGAAG 1 cut(s) 540
Eco81I CCTNAGG 1 cut(s) 432
Eco88I CYCGRG 2 cut(s) 191, 1008
Eco91I GGTNACC 1 cut(s) 85
EcoO65I GGTNACC 1 cut(s) 85
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 3 cut(s) 181, 300, 1025
EcoT14I CCWWGG 2 cut(s) 117, 791
EcoT22I ATGCAT 2 cut(s) 513, 625
ErhI CCWWGG 2 cut(s) 117, 791
FaeI CATG 1 cut(s) 515
FaqI GGGAC 1 cut(s) 322
FatI CATG 1 cut(s) 511
FauI CCCGC 3 cut(s) 261, 413, 857
FbaI TGATCA 1 cut(s) 9
FokI GGATG 4 cut(s) 19, 117, 463, 719
FspBI CTAG 3 cut(s) 441, 725, 755
GlaI GCGC 1 cut(s) 907
GsaI CCCAGC 1 cut(s) 104
GsuI CTGGAG 2 cut(s) 144, 323
HaeIII GGCC 6 cut(s) 50, 81, 203, 213, 232, 242
HhaI GCGC 1 cut(s) 908
Hin1II CATG 1 cut(s) 515
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 3 cut(s) 178, 269, 365
HphI GGTGA 4 cut(s) 28, 79, 187, 575
Hpy166II GTNNAC 2 cut(s) 332, 685
Hpy188I TCNGA 6 cut(s) 96, 387, 520, 535, 963, 973
Hpy188III TCNNGA 5 cut(s) 30, 317, 441, 644, 725
Hpy8I GTNNAC 2 cut(s) 332, 685
Hpy99I CGWCG 3 cut(s) 248, 363, 1022
HpyAV CCTTC 4 cut(s) 35, 119, 515, 893
HpyCH4III ACNGT 2 cut(s) 71, 590
HpyCH4V TGCA 9 cut(s) 332, 463, 511, 623, 703, 718, 737, 924, 930
HpyF10VI GCNNNNNNNGC 7 cut(s) 219, 389, 414, 629, 903, 1019, 1040
HpyF3I CTNAG 2 cut(s) 432, 532
Hsp92II CATG 1 cut(s) 515
HspAI GCGC 1 cut(s) 906
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
LmnI GCTCC 2 cut(s) 219, 861
Lsp1109I GCAGC 7 cut(s) 395, 895, 929, 971, 1000, 1024, 1027
LweI GCATC 5 cut(s) 48, 95, 610, 718, 893
MaeI CTAG 3 cut(s) 441, 725, 755
MaeIII GTNAC 3 cut(s) 85, 270, 709
MalI GATC 8 cut(s) 11, 28, 46, 93, 115, 471, 642, 723
MbiI CCGCTC 1 cut(s) 864
MboI GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
MboII GAAGA 2 cut(s) 36, 665
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 3 cut(s) 615, 635, 815
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 996
Mph1103I ATGCAT 2 cut(s) 513, 625
MseI TTAA 1 cut(s) 899
MslI CAYNNNNRTG 1 cut(s) 34
MspA1I CMGCKG 2 cut(s) 408, 1037
MspCI CTTAAG 1 cut(s) 898
MspR9I CCNGG 9 cut(s) 83, 136, 183, 192, 193, 199, 234, 302, 1027
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 3 cut(s) 183, 302, 1027
MwoI GCNNNNNNNGC 7 cut(s) 219, 389, 414, 629, 903, 1019, 1040
NciI CCSGG 6 cut(s) 83, 136, 192, 193, 199, 234
NdeII GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
NlaIII CATG 1 cut(s) 515
NlaIV GGNNCC 6 cut(s) 196, 202, 221, 490, 777, 869
NmeAIII GCCGAG 2 cut(s) 76, 142
NmuCI GTSAC 2 cut(s) 85, 270
NsiI ATGCAT 2 cut(s) 513, 625
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 2 cut(s) 178, 365
PflMI CCANNNNNTGG 1 cut(s) 512
PfoI TCCNGGA 3 cut(s) 181, 300, 1025
Ple19I CGATCG 1 cut(s) 94
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PsiI TTATAA 2 cut(s) 425, 572
Psp6I CCWGG 3 cut(s) 181, 300, 1025
PspEI GGTNACC 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 3 cut(s) 181, 300, 1025
PspN4I GGNNCC 6 cut(s) 196, 202, 221, 490, 777, 869
PspPI GGNCC 5 cut(s) 195, 201, 240, 776, 963
PstI CTGCAG 1 cut(s) 926
PvuI CGATCG 1 cut(s) 94
RsaI GTAC 1 cut(s) 840
RsaNI GTAC 1 cut(s) 839
RseI CAYNNNNRTG 1 cut(s) 34
SaqAI TTAA 1 cut(s) 899
Sau3AI GATC 8 cut(s) 9, 26, 44, 91, 113, 469, 640, 721
Sau96I GGNCC 5 cut(s) 195, 201, 240, 776, 963
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 9 cut(s) 83, 136, 183, 192, 193, 199, 234, 302, 1027
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 5 cut(s) 48, 95, 610, 718, 893
SfcI CTRYAG 1 cut(s) 922
SinI GGWCC 3 cut(s) 195, 776, 963
SmaI CCCGGG 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 34
SmlI CTYRAG 2 cut(s) 744, 898
SmoI CTYRAG 2 cut(s) 744, 898
Sse9I AATT 3 cut(s) 615, 635, 815
SspMI CTAG 3 cut(s) 441, 725, 755
StyD4I CCNGG 9 cut(s) 81, 134, 181, 190, 191, 197, 232, 300, 1025
StyI CCWWGG 2 cut(s) 117, 791
TaaI ACNGT 2 cut(s) 71, 590
TaqI TCGA 3 cut(s) 112, 639, 978
TasI AATT 3 cut(s) 615, 635, 815
TauI GCSGC 3 cut(s) 414, 417, 1037
TfiI GAWTC 2 cut(s) 178, 365
Tru1I TTAA 1 cut(s) 899
Tru9I TTAA 1 cut(s) 899
TscAI CASTG 2 cut(s) 136, 595
TseFI GTSAC 2 cut(s) 85, 270
TseI GCWGC 7 cut(s) 408, 908, 942, 984, 1013, 1037, 1040
Tsp45I GTSAC 2 cut(s) 85, 270
TspDTI ATGAA 1 cut(s) 800
TspGWI ACGGA 2 cut(s) 261, 411
TspMI CCCGGG 1 cut(s) 191
TspRI CASTG 2 cut(s) 136, 595
Van91I CCANNNNNTGG 1 cut(s) 512
Vha464I CTTAAG 1 cut(s) 898
VneI GTGCAC 1 cut(s) 330
VpaK11BI GGWCC 3 cut(s) 195, 776, 963
XapI RAATTY 1 cut(s) 635
XbaI TCTAGA 2 cut(s) 440, 724
XmaI CCCGGG 1 cut(s) 191
XspI CTAG 3 cut(s) 441, 725, 755
Zsp2I ATGCAT 2 cut(s) 513, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.