Rh5DG435300

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016455
Physical Location & Seq
Reverse (-)
69473023 .. 69479394
6372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG435300.1

Sequence Viewer

Length: 1146 bp
ATGGGCAAATTGCGACAAGAAATAAACAAGGTCATGCAGGAACAAGATACTGGTGTTGCTCTGCATTTGAAGAGGCTTACTGCTTTCCTTGGGCATTGCATAATTTCAAGGTATTGCAATGGAGTGGATTCCATCAGTGATTTTCGAGCAACTATTGTTAAAAATGTTCTGGTAAAGCTTTTGTGGCAAGAGTCCATTTTCTCTAGTAGCTCACAAAATAAGGAGCAAACATCCCTGGGATTATCAGCAGATTCATGTGAAATTGGTTTAGCTTCCAGCAAGAAGACAGTTGAAACATTGAAAATTACGTACCCAATGAGCTACTTTAGAGAGTTGGTAAACTGCATTGTTGGGGTTCTTTCAGGCATTTATTTGCTGGAGCATGATTTGCTCTTAGCTTTCTATGCAGAATTTCAGGAGAATTGTCAGAATTTCTTTCAGCATGCTAGTAACATGGAAAGAGAAAGTGAGTATGCCGAGCTAGTTATTCAGTTTATATTGTTACTTGGAGAACAAGCAATGCAGAATGGTGAAAGTTGGCCTTTGGCTTCTGGAGTTGGACCAATGGTGGAAGATACATTCCTGCATATGTTCAAAGCAAGTTTTGTTCGATGGTGTCTCAGTGGGAGTAGTTGCTCCATAAATGCTTGTCTTGACCTGTTACTTGCATTGCTTGACGATGAATATTTCTCCGAGCAGCGAGATAGTGTTATCAGATATGATACTAACCTGGAGCGTTCTGGATATGCACCCTGTTTTTTAGATTCTGACCGGATATTATATTCCATCACATTGACATCTTTGAGCTGCTTATGTAAGCTTGAAGTTCTGGACTGTCACTCTCAGGATCCCTATGAGAAACAAAATCTGCTAGACCAACTTCTTTGTGAAGGTGATGGACGGCCTTTGCCAGTTCTGGATTTCAGCAGGCCAGAGGGATTAGTTGCCAGGGACATATCTGCTGACCATGGGTCTTTTGGCTTGAGATATTTGTACACCCAACTGAAAGATTTCACAATTGGATGCTTGAATTCATTAGACAGTGCCAAAAAATTGTCCGAGTTGGATCAAGAAGACAAGTTTCTCAACGTACTGTATCCAAGTGGAAACGAGTTATGGAGACCACCTTCAAGAAGAAATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

381

Amino Acids

43.13

Weight (kDa)

4.93

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 842, 855, 1074
AcsI RAATTY 3 cut(s) 410, 430, 1030
AfaI GTAC 3 cut(s) 311, 995, 1092
AgsI TTSAA 8 cut(s) 70, 108, 293, 301, 595, 824, 1030, 1131
AhdI GACNNNNNGTC 1 cut(s) 970
AjnI CCWGG 3 cut(s) 234, 729, 947
AluBI AGCT 8 cut(s) 178, 210, 272, 321, 398, 481, 807, 820
AluI AGCT 8 cut(s) 178, 210, 272, 321, 398, 481, 807, 820
Alw26I GTCTC 2 cut(s) 623, 1114
AlwI GGATC 3 cut(s) 842, 855, 1074
AoxI GGCC 3 cut(s) 539, 902, 929
ApeKI GCWGC 2 cut(s) 697, 807
ApoI RAATTY 3 cut(s) 410, 430, 1030
Asp700I GAANNNNTTC 1 cut(s) 1010
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 2 cut(s) 542, 905
AvaII GGWCC 1 cut(s) 560
BaeI ACNNNNGTAYC 2 cut(s) 39, 72
BamHI GGATCC 1 cut(s) 847
BbsI GAAGAC 2 cut(s) 290, 1080
BbvI GCAGC 2 cut(s) 709, 794
BccI CCATC 4 cut(s) 140, 606, 794, 890
BceAI ACGGC 1 cut(s) 917
BciT130I CCWGG 3 cut(s) 236, 731, 949
BciVI GTATCC 1 cut(s) 1107
BcoDI GTCTC 2 cut(s) 623, 1114
BfaI CTAG 4 cut(s) 204, 447, 482, 872
BfuI GTATCC 1 cut(s) 1107
BisI GCNGC 2 cut(s) 698, 808
BlsI GCNGC 2 cut(s) 699, 809
Bme1390I CCNGG 3 cut(s) 236, 731, 949
Bme18I GGWCC 1 cut(s) 560
BmeRI GACNNNNNGTC 1 cut(s) 970
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 849
BmrFI CCNGG 3 cut(s) 236, 731, 949
BmsI GCATC 1 cut(s) 1013
BpiI GAAGAC 2 cut(s) 290, 1080
BpmI CTGGAG 3 cut(s) 398, 573, 752
BpuEI CTTGAG 1 cut(s) 1003
Bsa29I ATCGAT 1 cut(s) 1141
BsaAI YACGTR 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 1114
BsaJI CCNNGG 5 cut(s) 88, 234, 235, 948, 967
BsaWI WCCGGW 1 cut(s) 771
Bse1I ACTGG 2 cut(s) 55, 911
Bse3DI GCAATG 4 cut(s) 94, 124, 525, 668
BseBI CCWGG 3 cut(s) 236, 731, 949
BseCI ATCGAT 1 cut(s) 1141
BseDI CCNNGG 5 cut(s) 88, 234, 235, 948, 967
BseGI GGATG 2 cut(s) 230, 1028
BseMI GCAATG 4 cut(s) 94, 124, 525, 668
BseMII CTCAG 2 cut(s) 634, 857
BseNI ACTGG 2 cut(s) 55, 911
BseXI GCAGC 2 cut(s) 709, 794
BshFI GGCC 3 cut(s) 541, 904, 931
BshVI ATCGAT 1 cut(s) 1141
BsiSI CCGG 1 cut(s) 772
BslFI GGGAC 1 cut(s) 965
BsmAI GTCTC 2 cut(s) 623, 1114
BsmFI GGGAC 1 cut(s) 965
BsnI GGCC 3 cut(s) 541, 904, 931
Bso31I GGTCTC 1 cut(s) 1114
Bsp1407I TGTACA 1 cut(s) 993
Bsp143I GATC 2 cut(s) 847, 1066
Bsp19I CCATGG 1 cut(s) 967
BspANI GGCC 3 cut(s) 541, 904, 931
BspCNI CTCAG 2 cut(s) 633, 856
BspDI ATCGAT 1 cut(s) 1141
BspLI GGNNCC 1 cut(s) 849
BspPI GGATC 3 cut(s) 842, 855, 1074
BspTNI GGTCTC 1 cut(s) 1114
BsrDI GCAATG 4 cut(s) 94, 124, 525, 668
BsrGI TGTACA 1 cut(s) 993
BsrI ACTGG 2 cut(s) 55, 911
BssECI CCNNGG 5 cut(s) 88, 234, 235, 948, 967
BssMI GATC 2 cut(s) 847, 1066
BssT1I CCWWGG 2 cut(s) 88, 967
Bst2UI CCWGG 3 cut(s) 236, 731, 949
Bst4CI ACNGT 4 cut(s) 289, 836, 1043, 1095
Bst6I CTCTTC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 388
BstAUI TGTACA 1 cut(s) 993
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 444, 929
BstDEI CTNAG 3 cut(s) 394, 620, 843
BstDSI CCRYGG 1 cut(s) 967
BstF5I GGATG 2 cut(s) 230, 1028
BstKTI GATC 2 cut(s) 850, 1069
BstMAI GTCTC 2 cut(s) 623, 1114
BstMBI GATC 2 cut(s) 847, 1066
BstMWI GCNNNNNNNGC 3 cut(s) 184, 388, 404
BstNI CCWGG 3 cut(s) 236, 731, 949
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 3 cut(s) 234, 729, 947
BstSNI TACGTA 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 709, 794
BstV2I GAAGAC 2 cut(s) 290, 1080
BstX2I RGATCY 1 cut(s) 847
BstYI RGATCY 1 cut(s) 847
Bsu15I ATCGAT 1 cut(s) 1141
BsuI GTATCC 1 cut(s) 1107
BsuRI GGCC 3 cut(s) 541, 904, 931
BsuTUI ATCGAT 1 cut(s) 1141
BtgI CCRYGG 1 cut(s) 967
BtsCI GGATG 2 cut(s) 230, 1028
BtsIMutI CAGTG 3 cut(s) 142, 628, 1048
Cac8I GCNNGC 2 cut(s) 444, 929
Cfr13I GGNCC 1 cut(s) 560
ClaI ATCGAT 1 cut(s) 1141
Csp6I GTAC 3 cut(s) 310, 994, 1091
CviAII CATG 6 cut(s) 34, 255, 383, 443, 454, 968
CviQI GTAC 3 cut(s) 310, 994, 1091
DdeI CTNAG 3 cut(s) 394, 620, 843
DpnI GATC 2 cut(s) 849, 1068
DpnII GATC 2 cut(s) 847, 1066
DriI GACNNNNNGTC 1 cut(s) 970
Eam1104I CTCTTC 1 cut(s) 65
Eam1105I GACNNNNNGTC 1 cut(s) 970
EarI CTCTTC 1 cut(s) 65
Eco105I TACGTA 1 cut(s) 309
Eco130I CCWWGG 2 cut(s) 88, 967
Eco31I GGTCTC 1 cut(s) 1114
Eco47I GGWCC 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 1030
EcoRII CCWGG 3 cut(s) 234, 729, 947
EcoT14I CCWWGG 2 cut(s) 88, 967
ErhI CCWWGG 2 cut(s) 88, 967
FaeI CATG 6 cut(s) 37, 258, 386, 446, 457, 971
FalI AAGNNNNNCTT 2 cut(s) 526, 558
FaqI GGGAC 1 cut(s) 965
FatI CATG 6 cut(s) 33, 254, 382, 442, 453, 967
FauNDI CATATG 1 cut(s) 588
Fnu4HI GCNGC 2 cut(s) 698, 808
FokI GGATG 2 cut(s) 217, 1035
Fsp4HI GCNGC 2 cut(s) 698, 808
FspBI CTAG 4 cut(s) 204, 447, 482, 872
GluI GCNGC 2 cut(s) 698, 808
GsuI CTGGAG 3 cut(s) 398, 573, 752
HaeIII GGCC 3 cut(s) 541, 904, 931
HapII CCGG 1 cut(s) 772
Hin1II CATG 6 cut(s) 37, 258, 386, 446, 457, 971
HindIII AAGCTT 2 cut(s) 176, 818
HinfI GANTC 4 cut(s) 128, 191, 251, 764
HpaII CCGG 1 cut(s) 772
HphI GGTGA 2 cut(s) 542, 905
Hpy166II GTNNAC 2 cut(s) 340, 996
Hpy188I TCNGA 5 cut(s) 429, 694, 716, 769, 1060
Hpy188III TCNNGA 9 cut(s) 416, 552, 653, 741, 830, 845, 917, 1070, 1131
Hpy8I GTNNAC 2 cut(s) 340, 996
HpyAV CCTTC 2 cut(s) 884, 1137
HpyCH4III ACNGT 4 cut(s) 289, 836, 1043, 1095
HpyCH4IV ACGT 2 cut(s) 308, 1089
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 388, 404
HpyF3I CTNAG 3 cut(s) 394, 620, 843
HpySE526I ACGT 2 cut(s) 308, 1089
Hsp92II CATG 6 cut(s) 37, 258, 386, 446, 457, 971
Kzo9I GATC 2 cut(s) 847, 1066
LmnI GCTCC 4 cut(s) 223, 379, 641, 733
Lsp1109I GCAGC 2 cut(s) 709, 794
LweI GCATC 1 cut(s) 1013
MaeI CTAG 4 cut(s) 204, 447, 482, 872
MaeII ACGT 2 cut(s) 308, 1089
MaeIII GTNAC 4 cut(s) 449, 501, 660, 836
MalI GATC 2 cut(s) 849, 1068
MboI GATC 2 cut(s) 847, 1066
MboII GAAGA 5 cut(s) 82, 295, 584, 1085, 1146
MfeI CAATTG 1 cut(s) 1017
MflI RGATCY 1 cut(s) 847
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 538, 1044
MnlI CCTC 2 cut(s) 66, 928
MroXI GAANNNNTTC 1 cut(s) 1010
MseI TTAA 1 cut(s) 159
MspI CCGG 1 cut(s) 772
MspR9I CCNGG 3 cut(s) 236, 731, 949
MunI CAATTG 1 cut(s) 1017
MvaI CCWGG 3 cut(s) 236, 731, 949
MwoI GCNNNNNNNGC 3 cut(s) 184, 388, 404
NcoI CCATGG 1 cut(s) 967
NdeI CATATG 1 cut(s) 588
NdeII GATC 2 cut(s) 847, 1066
NlaIII CATG 6 cut(s) 37, 258, 386, 446, 457, 971
NlaIV GGNNCC 1 cut(s) 849
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 836
NspI RCATGY 1 cut(s) 446
PaeI GCATGC 1 cut(s) 446
PasI CCCWGGG 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 1010
PfeI GAWTC 3 cut(s) 128, 251, 764
PkrI GCNGC 2 cut(s) 699, 809
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 309
Psp6I CCWGG 3 cut(s) 234, 729, 947
PspGI CCWGG 3 cut(s) 234, 729, 947
PspN4I GGNNCC 1 cut(s) 849
PspPI GGNCC 1 cut(s) 560
PsuI RGATCY 1 cut(s) 847
RsaI GTAC 3 cut(s) 311, 995, 1092
RsaNI GTAC 3 cut(s) 310, 994, 1091
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 2 cut(s) 698, 808
Sau3AI GATC 2 cut(s) 847, 1066
Sau96I GGNCC 1 cut(s) 560
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 3 cut(s) 236, 731, 949
SfaNI GCATC 1 cut(s) 1013
SinI GGWCC 1 cut(s) 560
SmlI CTYRAG 1 cut(s) 982
SmoI CTYRAG 1 cut(s) 982
SnaBI TACGTA 1 cut(s) 309
SphI GCATGC 1 cut(s) 446
SspI AATATT 1 cut(s) 686
SspMI CTAG 4 cut(s) 204, 447, 482, 872
StyD4I CCNGG 3 cut(s) 234, 729, 947
StyI CCWWGG 2 cut(s) 88, 967
TaaI ACNGT 4 cut(s) 289, 836, 1043, 1095
TaiI ACGT 2 cut(s) 311, 1092
TaqI TCGA 3 cut(s) 145, 610, 1141
TatI WGTACW 1 cut(s) 993
TfiI GAWTC 3 cut(s) 128, 251, 764
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 3 cut(s) 142, 628, 1048
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 2 cut(s) 697, 807
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 3 cut(s) 243, 696, 1023
TspRI CASTG 3 cut(s) 142, 628, 1048
VpaK11BI GGWCC 1 cut(s) 560
XapI RAATTY 3 cut(s) 410, 430, 1030
XceI RCATGY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 974
XmnI GAANNNNTTC 1 cut(s) 1010
XspI CTAG 4 cut(s) 204, 447, 482, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.