Rmu_sc0000733.1_g000018

5'-nucleotidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000733.1
OG0002535
Physical Location & Seq
Reverse (-)
92337 .. 98719
6383 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1320 bp
atgagtaatcgcctcctcttcgttggcaccaccatttgctctaatcgctgcttcatcccacaccacttcacttccaggacctgcttttgtagtggtaccttgagaaatcagactaacatggaatccgatgaggttgtggtggtggctcaccctcaccttttggataccaaaattgctgctattcggaatgcgggtccccccaaactccaggttatagcagattttgatgccacactcacaaagtacagagtgaatggttctcggggacaaagcagtcatggccttttgcaacaggagaatcctgaatataataacaaaaggcgggagctattcgaatactatcatccattagagttctcgccagctattccaattgaacaaaaaactaagcttatggaggaatggtggggaaagactcattctcttttgattgagggaggccttacctatgatgggataaggcaatccgttgctgattccacaattgcttttagggagggtgttgttgaattatttgagtttttagagaaaagagacattcctgttcttatattttcagcaggacttgcagatatcatagaggaggtcctgaggcagaaagttcacagattgtttaaaaatgtgaagatcgtctccaataggatggtatttgatgataacagccaccttgtatcgtttaaagggaagacgattcatagtctaaataaaaatgagcacgctcttgatatggctgctccacttcatgagcaattaggtgatttagatgggccaacttatgagaatgcgtcagtcaagacaagaagaaatgtcattcttctgggcgatcatattggtgacctgggaatgtctgatggtttgaactatgagaatagaatttccgtgggatttctgaatgacaatgttgagaattctattgatagctaccgcgaagcctttgacgtcgtttacctgaatgatgcacccatgtggggagtagtaaagcttgtttcacaactatgtcctggagatgctccaaaagaaagcccaaactccaggtccagcaggttgctacagcaagcccaaacctatctccaaacgcgaagtggccgtatagcacctccgccgcgtcgacccacatcctcgccggctagaaccccgcgtcccacacctgcctacgttgtcgcgcaatcctacagccaccaagtcgacgccggatcagccaaaaaacctcgccgccgcatagcccccacgaaccttatcgccgctcagtccattcgacccacgcagccgctcaagccatccctgcacaacccaaccaccacgcatagagcctacatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko00760 ko01100 ko01110 map00230 map00240 map00760 map01100 map01110
Pfam Domains
Protein Families
UMPH-1

Protein Analysis

439

Amino Acids

49.3

Weight (kDa)

8.94

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UMPH-1 PF05822 78 - 332 1.2e-91 Pyrimidine 5'-nucleotidase (UMPH-1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1156
AasI GACNNNNNNGTC 1 cut(s) 273
AatII GACGTC 1 cut(s) 942
Acc36I ACCTGC 3 cut(s) 89, 1032, 1156
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 26, 95
AccBSI CCGCTC 2 cut(s) 1244, 1270
AccI GTMKAC 2 cut(s) 1108, 1185
AccII CGCG 5 cut(s) 927, 1078, 1105, 1138, 1163
AclWI GGATC 1 cut(s) 1201
AcoI YGGCCR 1 cut(s) 1084
AcsI RAATTY 2 cut(s) 873, 907
AcyI GRCGYC 2 cut(s) 939, 1188
AfaI GTAC 2 cut(s) 97, 245
AfiI CCNNNNNNNGG 2 cut(s) 453, 968
AgsI TTSAA 3 cut(s) 377, 509, 859
AjnI CCWGG 5 cut(s) 74, 207, 837, 1000, 1031
AleI CACNNNNGTG 1 cut(s) 964
AloI GAACNNNNNNTCC 2 cut(s) 1123, 1155
AluBI AGCT 5 cut(s) 328, 365, 391, 921, 982
AluI AGCT 5 cut(s) 328, 365, 391, 921, 982
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 2 cut(s) 528, 637
AlwI GGATC 1 cut(s) 1201
AlwNI CAGNNNCTG 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 4 cut(s) 280, 439, 767, 1084
ApeKI GCWGC 4 cut(s) 48, 176, 731, 1264
ApoI RAATTY 2 cut(s) 873, 907
ArsI GACNNNNNNTTYG 2 cut(s) 1019, 1051
Asp718I GGTACC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 1165
AspS9I GGNCC 5 cut(s) 78, 194, 586, 767, 1035
AsuHPI GGTGA 4 cut(s) 140, 146, 767, 845
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 4 cut(s) 78, 194, 586, 1035
AxyI CCTNAGG 1 cut(s) 590
BanI GGYRCC 2 cut(s) 26, 95
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 4 cut(s) 35, 163, 718, 1276
BccI CCATC 5 cut(s) 446, 637, 758, 845, 1285
BceAI ACGGC 1 cut(s) 1071
BciT130I CCWGG 5 cut(s) 76, 209, 839, 1002, 1033
BciVI GTATCC 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 528, 637
BfaI CTAG 1 cut(s) 1128
BfmI CTRYAG 2 cut(s) 1049, 1171
BfuAI ACCTGC 3 cut(s) 89, 1032, 1156
BfuI GTATCC 1 cut(s) 157
BisI GCNGC 9 cut(s) 49, 177, 732, 1103, 1213, 1216, 1242, 1265, 1268
BlsI GCNGC 9 cut(s) 50, 178, 733, 1104, 1214, 1217, 1243, 1266, 1269
Bme1390I CCNGG 5 cut(s) 76, 209, 839, 1002, 1033
Bme18I GGWCC 4 cut(s) 78, 194, 586, 1035
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 5 cut(s) 78, 194, 586, 767, 1035
BmiI GGNNCC 4 cut(s) 28, 97, 195, 196
BmrFI CCNGG 5 cut(s) 76, 209, 839, 1002, 1033
BmsI GCATC 3 cut(s) 217, 946, 997
BpiI GAAGAC 1 cut(s) 692
BpmI CTGGAG 3 cut(s) 191, 1015, 1023
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 2 cut(s) 121, 1256
BsaHI GRCGYC 2 cut(s) 939, 1188
BsaJI CCNNGG 2 cut(s) 838, 879
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 968
Bse118I RCCGGY 1 cut(s) 1123
Bse21I CCTNAGG 1 cut(s) 590
BseBI CCWGG 5 cut(s) 76, 209, 839, 1002, 1033
BseDI CCNNGG 2 cut(s) 838, 879
BseGI GGATG 5 cut(s) 54, 343, 648, 1115, 1277
BseLI CCNNNNNNNGG 2 cut(s) 453, 968
BseMII CTCAG 2 cut(s) 581, 1259
BseRI GAGGAG 2 cut(s) 5, 596
BseXI GCAGC 4 cut(s) 35, 163, 718, 1276
BsgI GTGCAG 1 cut(s) 1268
Bsh1236I CGCG 5 cut(s) 927, 1078, 1105, 1138, 1163
BshFI GGCC 4 cut(s) 282, 441, 769, 1086
BshNI GGYRCC 2 cut(s) 26, 95
BsiHKAI GWGCWC 1 cut(s) 717
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 1124, 1191
BslFI GGGAC 3 cut(s) 180, 279, 1125
BslI CCNNNNNNNGG 2 cut(s) 453, 968
BsmAI GTCTC 2 cut(s) 528, 637
BsmBI CGTCTC 1 cut(s) 637
BsmFI GGGAC 3 cut(s) 180, 279, 1125
BsmI GAATGC 2 cut(s) 193, 787
BsnI GGCC 4 cut(s) 282, 441, 769, 1086
BsoBI CYCGRG 1 cut(s) 261
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 3 cut(s) 627, 823, 1193
BspANI GGCC 4 cut(s) 282, 441, 769, 1086
BspCNI CTCAG 2 cut(s) 582, 1258
BspFNI CGCG 5 cut(s) 927, 1078, 1105, 1138, 1163
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 4 cut(s) 28, 97, 195, 196
BspMI ACCTGC 3 cut(s) 89, 1032, 1156
BspPI GGATC 1 cut(s) 1201
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 2 cut(s) 26, 95
BsrBI CCGCTC 2 cut(s) 1244, 1270
BsrFI RCCGGY 1 cut(s) 1123
BssAI RCCGGY 1 cut(s) 1123
BssECI CCNNGG 2 cut(s) 838, 879
BssMI GATC 3 cut(s) 627, 823, 1193
BssNI GRCGYC 2 cut(s) 939, 1188
Bst2UI CCWGG 5 cut(s) 76, 209, 839, 1002, 1033
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 2 cut(s) 939, 1188
BstAPI GCANNNNNTGC 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 4 cut(s) 363, 717, 1056, 1125
BstDEI CTNAG 3 cut(s) 387, 590, 1245
BstDSI CCRYGG 1 cut(s) 879
BstEII GGTNACC 1 cut(s) 833
BstF5I GGATG 5 cut(s) 54, 343, 648, 1115, 1277
BstFNI CGCG 5 cut(s) 927, 1078, 1105, 1138, 1163
BstHHI GCGC 1 cut(s) 1165
BstKTI GATC 3 cut(s) 630, 826, 1196
BstMAI GTCTC 2 cut(s) 528, 637
BstMBI GATC 3 cut(s) 627, 823, 1193
BstMWI GCNNNNNNNGC 6 cut(s) 45, 279, 566, 1196, 1273, 1282
BstNI CCWGG 5 cut(s) 76, 209, 839, 1002, 1033
BstPI GGTNACC 1 cut(s) 833
BstSCI CCNGG 5 cut(s) 74, 207, 837, 1000, 1031
BstSFI CTRYAG 2 cut(s) 1049, 1171
BstUI CGCG 5 cut(s) 927, 1078, 1105, 1138, 1163
BstV1I GCAGC 4 cut(s) 35, 163, 718, 1276
BstV2I GAAGAC 1 cut(s) 692
BstXI CCANNNNNNTGG 1 cut(s) 643
Bsu36I CCTNAGG 1 cut(s) 590
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 4 cut(s) 282, 441, 769, 1086
BtgI CCRYGG 1 cut(s) 879
BtsCI GGATG 5 cut(s) 54, 343, 648, 1115, 1277
BveI ACCTGC 3 cut(s) 89, 1032, 1156
Cac8I GCNNGC 4 cut(s) 363, 717, 1056, 1125
CaiI CAGNNNCTG 1 cut(s) 81
CciI TCATGA 1 cut(s) 742
CfoI GCGC 1 cut(s) 1165
Cfr10I RCCGGY 1 cut(s) 1123
Cfr13I GGNCC 5 cut(s) 78, 194, 586, 767, 1035
CseI GACGC 4 cut(s) 774, 1094, 1127, 1196
Csp6I GTAC 2 cut(s) 96, 244
CviAII CATG 4 cut(s) 118, 278, 743, 964
CviQI GTAC 2 cut(s) 96, 244
DdeI CTNAG 3 cut(s) 387, 590, 1245
DpnI GATC 3 cut(s) 629, 825, 1195
DpnII GATC 3 cut(s) 627, 823, 1193
DraI TTTAAA 2 cut(s) 616, 679
DrdI GACNNNNNNGTC 1 cut(s) 273
DseDI GACNNNNNNGTC 1 cut(s) 273
EaeI YGGCCR 1 cut(s) 1084
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 1089
Eco147I AGGCCT 1 cut(s) 441
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 4 cut(s) 78, 194, 586, 1035
Eco81I CCTNAGG 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 833
EcoO109I RGGNCCY 3 cut(s) 78, 194, 586
EcoO65I GGTNACC 1 cut(s) 833
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 5 cut(s) 74, 207, 837, 1000, 1031
EcoRV GATATC 1 cut(s) 574
Esp3I CGTCTC 1 cut(s) 637
FaeI CATG 4 cut(s) 121, 281, 746, 967
FaqI GGGAC 3 cut(s) 180, 279, 1125
FatI CATG 4 cut(s) 117, 277, 742, 963
FauI CCCGC 3 cut(s) 184, 315, 1143
FblI GTMKAC 2 cut(s) 1108, 1185
Fnu4HI GCNGC 9 cut(s) 49, 177, 732, 1103, 1213, 1216, 1242, 1265, 1268
FokI GGATG 5 cut(s) 41, 330, 655, 1102, 1264
Fsp4HI GCNGC 9 cut(s) 49, 177, 732, 1103, 1213, 1216, 1242, 1265, 1268
FspBI CTAG 1 cut(s) 1128
GlaI GCGC 1 cut(s) 1164
GluI GCNGC 9 cut(s) 49, 177, 732, 1103, 1213, 1216, 1242, 1265, 1268
GsuI CTGGAG 3 cut(s) 191, 1015, 1023
HaeIII GGCC 4 cut(s) 282, 441, 769, 1086
HapII CCGG 2 cut(s) 1124, 1191
HgaI GACGC 4 cut(s) 774, 1094, 1127, 1196
HhaI GCGC 1 cut(s) 1165
Hin1I GRCGYC 2 cut(s) 939, 1188
Hin1II CATG 4 cut(s) 121, 281, 746, 967
Hin6I GCGC 1 cut(s) 1163
HinP1I GCGC 1 cut(s) 1163
HincII GTYRAC 2 cut(s) 1109, 1186
HindII GTYRAC 2 cut(s) 1109, 1186
HindIII AAGCTT 2 cut(s) 389, 980
HinfI GANTC 5 cut(s) 122, 298, 415, 476, 691
HpaII CCGG 2 cut(s) 1124, 1191
HphI GGTGA 4 cut(s) 140, 146, 767, 845
Hpy166II GTNNAC 4 cut(s) 604, 946, 1109, 1186
Hpy188I TCNGA 6 cut(s) 111, 127, 186, 850, 891, 1319
Hpy188III TCNNGA 5 cut(s) 302, 589, 722, 743, 793
Hpy8I GTNNAC 4 cut(s) 604, 946, 1109, 1186
Hpy99I CGWCG 3 cut(s) 944, 1110, 1190
HpyCH4IV ACGT 2 cut(s) 939, 1155
HpyCH4V TGCA 4 cut(s) 289, 569, 959, 1285
HpyF10VI GCNNNNNNNGC 6 cut(s) 45, 279, 566, 1196, 1273, 1282
HpyF3I CTNAG 3 cut(s) 387, 590, 1245
HpySE526I ACGT 2 cut(s) 939, 1155
Hsp92I GRCGYC 2 cut(s) 939, 1188
Hsp92II CATG 4 cut(s) 121, 281, 746, 967
HspAI GCGC 1 cut(s) 1163
KflI GGGWCCC 1 cut(s) 194
KpnI GGTACC 1 cut(s) 99
KroI GCCGGC 1 cut(s) 1123
KroNI GCCGGC 1 cut(s) 1125
Kzo9I GATC 3 cut(s) 627, 823, 1193
LmnI GCTCC 3 cut(s) 325, 739, 1015
Lsp1109I GCAGC 4 cut(s) 35, 163, 718, 1276
LweI GCATC 3 cut(s) 217, 946, 997
MaeI CTAG 1 cut(s) 1128
MaeII ACGT 2 cut(s) 939, 1155
MaeIII GTNAC 1 cut(s) 833
MalI GATC 3 cut(s) 629, 825, 1195
MbiI CCGCTC 2 cut(s) 1244, 1270
MboI GATC 3 cut(s) 627, 823, 1193
MboII GAAGA 5 cut(s) 10, 637, 697, 806, 813
MfeI CAATTG 2 cut(s) 372, 483
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 907
MlyI GAGTC 1 cut(s) 409
MroNI GCCGGC 1 cut(s) 1123
MseI TTAA 2 cut(s) 615, 678
MslI CAYNNNNRTG 3 cut(s) 831, 964, 994
MspI CCGG 2 cut(s) 1124, 1191
MspR9I CCNGG 5 cut(s) 76, 209, 839, 1002, 1033
MunI CAATTG 2 cut(s) 372, 483
Mva1269I GAATGC 2 cut(s) 193, 787
MvaI CCWGG 5 cut(s) 76, 209, 839, 1002, 1033
MvnI CGCG 5 cut(s) 927, 1078, 1105, 1138, 1163
MwoI GCNNNNNNNGC 6 cut(s) 45, 279, 566, 1196, 1273, 1282
NaeI GCCGGC 1 cut(s) 1125
NdeII GATC 3 cut(s) 627, 823, 1193
NgoMIV GCCGGC 1 cut(s) 1123
NlaIII CATG 4 cut(s) 121, 281, 746, 967
NlaIV GGNNCC 4 cut(s) 28, 97, 195, 196
NmuCI GTSAC 1 cut(s) 833
NspV TTCGAA 1 cut(s) 333
OliI CACNNNNGTG 1 cut(s) 964
PagI TCATGA 1 cut(s) 742
PaqCI CACCTGC 1 cut(s) 1156
PceI AGGCCT 1 cut(s) 441
PctI GAATGC 2 cut(s) 193, 787
PdiI GCCGGC 1 cut(s) 1125
PfeI GAWTC 4 cut(s) 122, 298, 476, 691
PfoI TCCNGGA 2 cut(s) 74, 1000
PkrI GCNGC 9 cut(s) 50, 178, 733, 1104, 1214, 1217, 1243, 1266, 1269
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
PpuMI RGGWCCY 3 cut(s) 78, 194, 586
Psp5II RGGWCCY 3 cut(s) 78, 194, 586
Psp6I CCWGG 5 cut(s) 74, 207, 837, 1000, 1031
PspEI GGTNACC 1 cut(s) 833
PspGI CCWGG 5 cut(s) 74, 207, 837, 1000, 1031
PspN4I GGNNCC 4 cut(s) 28, 97, 195, 196
PspPI GGNCC 5 cut(s) 78, 194, 586, 767, 1035
PspPPI RGGWCCY 3 cut(s) 78, 194, 586
PstNI CAGNNNCTG 1 cut(s) 81
RsaI GTAC 2 cut(s) 97, 245
RsaNI GTAC 2 cut(s) 96, 244
RseI CAYNNNNRTG 3 cut(s) 831, 964, 994
SalI GTCGAC 2 cut(s) 1107, 1184
SaqAI TTAA 2 cut(s) 615, 678
SatI GCNGC 9 cut(s) 49, 177, 732, 1103, 1213, 1216, 1242, 1265, 1268
Sau3AI GATC 3 cut(s) 627, 823, 1193
Sau96I GGNCC 5 cut(s) 78, 194, 586, 767, 1035
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 5 cut(s) 76, 209, 839, 1002, 1033
SduI GDGCHC 1 cut(s) 717
SfaNI GCATC 3 cut(s) 217, 946, 997
SfcI CTRYAG 2 cut(s) 1049, 1171
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 4 cut(s) 78, 194, 586, 1035
SmiMI CAYNNNNRTG 3 cut(s) 831, 964, 994
SmlI CTYRAG 2 cut(s) 100, 1271
SmoI CTYRAG 2 cut(s) 100, 1271
Sse9I AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 907
SseBI AGGCCT 1 cut(s) 441
SspMI CTAG 1 cut(s) 1128
StuI AGGCCT 1 cut(s) 441
StyD4I CCNGG 5 cut(s) 74, 207, 837, 1000, 1031
TaiI ACGT 2 cut(s) 942, 1158
TaqI TCGA 4 cut(s) 333, 1108, 1185, 1255
TasI AATT 7 cut(s) 171, 372, 483, 509, 749, 873, 907
TatI WGTACW 1 cut(s) 243
TauI GCSGC 5 cut(s) 1105, 1215, 1218, 1244, 1270
TfiI GAWTC 4 cut(s) 122, 298, 476, 691
Tru1I TTAA 2 cut(s) 615, 678
Tru9I TTAA 2 cut(s) 615, 678
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 4 cut(s) 48, 176, 731, 1264
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 3 cut(s) 43, 683, 731
TspGWI ACGGA 2 cut(s) 457, 868
VpaK11BI GGWCC 4 cut(s) 78, 194, 586, 1035
XapI RAATTY 2 cut(s) 873, 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 1079
XmiI GTMKAC 2 cut(s) 1108, 1185
XspI CTAG 1 cut(s) 1128
ZraI GACGTC 1 cut(s) 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.