Rmu_sc0000860.1_g000004

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000860.1
OG0008999
Physical Location & Seq
Reverse (-)
25946 .. 27772
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ctgggtgtttcagaatccggtaaagacggagaaaagaagagggtggtggaagaacagaagggggtgtattggacatttcccttttcttcatcactgttcattgccattttggtttatgaggcgaggtttgttttcaaaatcatgtctgatgggtattacagttccaagaagacagatgatatatgcgacgacgtttgtggagagggatcagaagcagcattgagcatgtccagactacgatgcgttttgcgggggttcgatttgaagacctatattttcgtttttgcagttcttccattatgcatcttcggtgtgtatttacatgggcagaaggtttcgtattttttacgacctctttgggaatctcctccgaagtctttcattgaacttccacactattatcatgagaatgtttcaatggagactctctgcagacttcatgggtggaagactcgtgagtcaccaagacgagtttatgatgcagttctcttcagtaatgaggtagacatccttacaattcggtggaatgaattatatccgtatgtcacccagtttgtgctgctggaatcaaactcaacatttactggcctgcccaaaccattggtttttgctaagaaccgggacaagtttaagtttgttgaagaccggctcacttatgtgacaactggaggaagatttaagaaaggcgagaacccattttatgaggaggcatttcaaagagtagcactagaccagcttctgagagtagccggtatagaagatgatgatctgctgataatgtctgatgttgacgagattcctagtgctcacacaattaatctcttgaggtggtgcgatgacatcccacctgttgttcatctcaggctgaggaactacttgtactctttcgagtattatgttgataaaaagagctggagggcatcagttcacaggtatcaaactggtaatactacatatgcgcacttcagacagagtgactaccttttgtcagatgcagggtggcattgtagcttttgtttccgccatatcagtgagttcatattcaagatgaaagcttacagccactatgatcgggtgaggttctctcattacttgaaccctaaaaggattcaggatgttatatgcaaaggggccgacctatttgacatgattcctgaggagtacacgtttaaggagattattggaaagatgggacctattcctagatcttattcagcagttcatcttcctgcatatttgctgaacaatgctgagaagtacaagtacctgctgcctggaaactgcaagagggaaaatggctga

Protein Analysis

433

Amino Acids

50.67

Weight (kDa)

7.97

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_17 PF04724 86 - 431 1e-186 Glycosyltransferase family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 457
Acc16I TGCGCA 1 cut(s) 960
Acc36I ACCTGC 1 cut(s) 1275
AccI GTMKAC 1 cut(s) 504
AciI CCGC 2 cut(s) 250, 1021
AclWI GGATC 1 cut(s) 214
AcuI CTGAAG 2 cut(s) 475, 949
AfaI GTAC 4 cut(s) 881, 1163, 1259, 1265
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 8 cut(s) 136, 265, 386, 417, 641, 716, 1045, 1096
AjnI CCWGG 1 cut(s) 1273
AleI CACNNNNGTG 1 cut(s) 656
AluBI AGCT 4 cut(s) 736, 912, 1011, 1055
AluI AGCT 4 cut(s) 736, 912, 1011, 1055
Alw21I GWGCWC 1 cut(s) 808
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 1 cut(s) 214
AlwNI CAGNNNCTG 1 cut(s) 739
AoxI GGCC 2 cut(s) 586, 1131
ApeKI GCWGC 3 cut(s) 215, 559, 1270
AseI ATTAAT 1 cut(s) 816
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 1 cut(s) 961
AspS9I GGNCC 2 cut(s) 1131, 1193
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 3 cut(s) 453, 538, 1087
AvaII GGWCC 1 cut(s) 1193
AxyI CCTNAGG 1 cut(s) 1155
BarI GAAGNNNNNNTAC 2 cut(s) 1247, 1279
BauI CACGAG 1 cut(s) 453
BbsI GAAGAC 4 cut(s) 176, 272, 455, 648
Bbv12I GWGCWC 1 cut(s) 808
BbvCI CCTCAGC 1 cut(s) 866
BbvI GCAGC 3 cut(s) 227, 546, 1257
BccI CCATC 2 cut(s) 143, 1183
BciT130I CCWGG 1 cut(s) 1275
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 3 cut(s) 728, 801, 1203
BfmI CTRYAG 1 cut(s) 430
BfuAI ACCTGC 1 cut(s) 1275
BglII AGATCT 1 cut(s) 1205
BisI GCNGC 3 cut(s) 216, 560, 1271
BlsI GCNGC 3 cut(s) 217, 561, 1272
Bme1390I CCNGG 2 cut(s) 620, 1275
Bme18I GGWCC 1 cut(s) 1193
BmgT120I GGNCC 2 cut(s) 1131, 1193
BmiI GGNNCC 2 cut(s) 1132, 1194
BmrFI CCNGG 2 cut(s) 620, 1275
BmrI ACTGGG 1 cut(s) 544
BmsI GCATC 5 cut(s) 230, 312, 469, 929, 982
BmuI ACTGGG 1 cut(s) 544
BpiI GAAGAC 4 cut(s) 176, 272, 455, 648
BplI GAGNNNNNCTC 2 cut(s) 1069, 1101
BpmI CTGGAG 2 cut(s) 687, 934
Bpu10I CCTNAGC 1 cut(s) 866
BpuEI CTTGAG 1 cut(s) 844
BpuMI CCSGG 1 cut(s) 620
BsaBI GATNNNNATC 1 cut(s) 765
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 1151, 1181
Bse118I RCCGGY 2 cut(s) 645, 749
Bse1I ACTGG 4 cut(s) 550, 589, 670, 946
Bse21I CCTNAGG 1 cut(s) 1155
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 765
BseBI CCWGG 1 cut(s) 1275
BseGI GGATG 3 cut(s) 507, 840, 1120
BseJI GATNNNNATC 1 cut(s) 765
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 5 cut(s) 731, 857, 874, 1146, 1242
BseNI ACTGG 4 cut(s) 550, 589, 670, 946
BseRI GAGGAG 3 cut(s) 357, 719, 1172
BseXI GCAGC 3 cut(s) 227, 546, 1257
BshFI GGCC 2 cut(s) 588, 1133
BsiHKAI GWGCWC 1 cut(s) 808
BsiSI CCGG 4 cut(s) 18, 619, 646, 750
BslFI GGGAC 2 cut(s) 635, 1206
BsmAI GTCTC 1 cut(s) 416
BsmFI GGGAC 2 cut(s) 635, 1206
BsnI GGCC 2 cut(s) 588, 1133
Bsp1286I GDGCHC 1 cut(s) 808
Bsp143I GATC 4 cut(s) 206, 766, 1069, 1205
BspACI CCGC 2 cut(s) 250, 1021
BspANI GGCC 2 cut(s) 588, 1133
BspCNI CTCAG 5 cut(s) 732, 858, 873, 1147, 1243
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 1132, 1194
BspMAI CTGCAG 1 cut(s) 434
BspMI ACCTGC 1 cut(s) 1275
BspPI GGATC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 2 cut(s) 645, 749
BsrI ACTGG 4 cut(s) 550, 589, 670, 946
BssAI RCCGGY 2 cut(s) 645, 749
BssMI GATC 4 cut(s) 206, 766, 1069, 1205
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 1275
Bst4CI ACNGT 2 cut(s) 96, 161
Bst6I CTCTTC 2 cut(s) 32, 494
BstC8I GCNNGC 1 cut(s) 590
BstDEI CTNAG 6 cut(s) 612, 740, 860, 866, 1155, 1251
BstF5I GGATG 3 cut(s) 507, 840, 1120
BstHHI GCGC 1 cut(s) 961
BstKTI GATC 4 cut(s) 209, 769, 1072, 1208
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 4 cut(s) 206, 766, 1069, 1205
BstNI CCWGG 1 cut(s) 1275
BstNSI RCATGY 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 618, 1273
BstSFI CTRYAG 1 cut(s) 430
BstV1I GCAGC 3 cut(s) 227, 546, 1257
BstV2I GAAGAC 4 cut(s) 176, 272, 455, 648
BstX2I RGATCY 1 cut(s) 1205
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 1205
Bsu36I CCTNAGG 1 cut(s) 1155
BsuRI GGCC 2 cut(s) 588, 1133
BtgZI GCGATG 1 cut(s) 849
BtsCI GGATG 3 cut(s) 507, 840, 1120
BtsIMutI CAGTG 2 cut(s) 92, 1036
BveI ACCTGC 1 cut(s) 1275
Cac8I GCNNGC 1 cut(s) 590
CaiI CAGNNNCTG 1 cut(s) 739
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 961
Cfr10I RCCGGY 2 cut(s) 645, 749
Cfr13I GGNCC 2 cut(s) 1131, 1193
Csp6I GTAC 4 cut(s) 880, 1162, 1258, 1264
CviAII CATG 6 cut(s) 142, 226, 323, 404, 440, 1147
CviQI GTAC 4 cut(s) 880, 1162, 1258, 1264
DdeI CTNAG 6 cut(s) 612, 740, 860, 866, 1155, 1251
DpnI GATC 4 cut(s) 208, 768, 1071, 1207
DpnII GATC 4 cut(s) 206, 766, 1069, 1205
DrdI GACNNNNNNGTC 1 cut(s) 457
DseDI GACNNNNNNGTC 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 32, 494
EarI CTCTTC 2 cut(s) 32, 494
EciI GGCGGA 1 cut(s) 1010
Eco47I GGWCC 1 cut(s) 1193
Eco57I CTGAAG 2 cut(s) 475, 949
Eco81I CCTNAGG 1 cut(s) 1155
EcoO109I RGGNCCY 1 cut(s) 1193
EcoRII CCWGG 1 cut(s) 1273
EcoT22I ATGCAT 1 cut(s) 305
FaeI CATG 6 cut(s) 145, 229, 326, 407, 443, 1150
FaqI GGGAC 2 cut(s) 635, 1206
FatI CATG 6 cut(s) 141, 225, 322, 403, 439, 1146
FauI CCCGC 1 cut(s) 243
FauNDI CATATG 1 cut(s) 955
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 216, 560, 1271
FokI GGATG 3 cut(s) 494, 827, 1127
Fsp4HI GCNGC 3 cut(s) 216, 560, 1271
FspAI RTGCGCAY 1 cut(s) 960
FspBI CTAG 3 cut(s) 728, 801, 1203
FspI TGCGCA 1 cut(s) 960
GlaI GCGC 1 cut(s) 960
GluI GCNGC 3 cut(s) 216, 560, 1271
GsuI CTGGAG 2 cut(s) 687, 934
HaeIII GGCC 2 cut(s) 588, 1133
HapII CCGG 4 cut(s) 18, 619, 646, 750
HhaI GCGC 1 cut(s) 961
Hin1II CATG 6 cut(s) 145, 229, 326, 407, 443, 1150
Hin6I GCGC 1 cut(s) 959
HinP1I GCGC 1 cut(s) 959
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 1 cut(s) 1053
HinfI GANTC 9 cut(s) 14, 362, 424, 451, 458, 566, 796, 1108, 1150
HpaII CCGG 4 cut(s) 18, 619, 646, 750
HphI GGTGA 3 cut(s) 453, 538, 1087
Hpy166II GTNNAC 4 cut(s) 505, 790, 928, 1164
Hpy188I TCNGA 8 cut(s) 13, 148, 211, 372, 741, 784, 968, 991
Hpy188III TCNNGA 7 cut(s) 231, 404, 455, 823, 1045, 1112, 1154
Hpy8I GTNNAC 4 cut(s) 505, 790, 928, 1164
Hpy99I CGWCG 2 cut(s) 191, 194
HpyAV CCTTC 2 cut(s) 52, 325
HpyCH4III ACNGT 2 cut(s) 96, 161
HpyCH4IV ACGT 2 cut(s) 192, 1166
HpyCH4V TGCA 8 cut(s) 287, 303, 432, 482, 995, 1125, 1232, 1284
HpyF3I CTNAG 6 cut(s) 612, 740, 860, 866, 1155, 1251
HpySE526I ACGT 2 cut(s) 192, 1166
Hsp92II CATG 6 cut(s) 145, 229, 326, 407, 443, 1150
HspAI GCGC 1 cut(s) 959
Kzo9I GATC 4 cut(s) 206, 766, 1069, 1205
Lsp1109I GCAGC 3 cut(s) 227, 546, 1257
LweI GCATC 5 cut(s) 230, 312, 469, 929, 982
MaeI CTAG 3 cut(s) 728, 801, 1203
MaeII ACGT 2 cut(s) 192, 1166
MaeIII GTNAC 4 cut(s) 459, 544, 658, 974
MalI GATC 4 cut(s) 208, 768, 1071, 1207
MboI GATC 4 cut(s) 206, 766, 1069, 1205
MflI RGATCY 1 cut(s) 1205
MhlI GDGCHC 1 cut(s) 808
MluCI AATT 3 cut(s) 516, 530, 813
MlyI GAGTC 3 cut(s) 418, 445, 467
Mph1103I ATGCAT 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 4 cut(s) 630, 678, 816, 1170
MslI CAYNNNNRTG 3 cut(s) 408, 656, 1029
MspI CCGG 4 cut(s) 18, 619, 646, 750
MspR9I CCNGG 2 cut(s) 620, 1275
MvaI CCWGG 1 cut(s) 1275
NciI CCSGG 1 cut(s) 620
NdeI CATATG 1 cut(s) 955
NdeII GATC 4 cut(s) 206, 766, 1069, 1205
NlaIII CATG 6 cut(s) 145, 229, 326, 407, 443, 1150
NlaIV GGNNCC 2 cut(s) 1132, 1194
NmuCI GTSAC 4 cut(s) 459, 544, 658, 974
NsbI TGCGCA 1 cut(s) 960
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 656
PagI TCATGA 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 6 cut(s) 14, 362, 566, 796, 1108, 1150
PkrI GCNGC 3 cut(s) 217, 561, 1272
PleI GAGTC 3 cut(s) 418, 445, 466
PpsI GAGTC 3 cut(s) 418, 445, 466
PpuMI RGGWCCY 1 cut(s) 1193
PshBI ATTAAT 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 1193
Psp6I CCWGG 1 cut(s) 1273
PspGI CCWGG 1 cut(s) 1273
PspN4I GGNNCC 2 cut(s) 1132, 1194
PspPI GGNCC 2 cut(s) 1131, 1193
PspPPI RGGWCCY 1 cut(s) 1193
PsrI GAACNNNNNNTAC 2 cut(s) 863, 895
PstI CTGCAG 1 cut(s) 434
PstNI CAGNNNCTG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 1205
RsaI GTAC 4 cut(s) 881, 1163, 1259, 1265
RsaNI GTAC 4 cut(s) 880, 1162, 1258, 1264
RseI CAYNNNNRTG 3 cut(s) 408, 656, 1029
SaqAI TTAA 4 cut(s) 630, 678, 816, 1170
SatI GCNGC 3 cut(s) 216, 560, 1271
Sau3AI GATC 4 cut(s) 206, 766, 1069, 1205
Sau96I GGNCC 2 cut(s) 1131, 1193
SchI GAGTC 3 cut(s) 418, 445, 467
ScrFI CCNGG 2 cut(s) 620, 1275
SduI GDGCHC 1 cut(s) 808
SfaNI GCATC 5 cut(s) 230, 312, 469, 929, 982
SfcI CTRYAG 1 cut(s) 430
SinI GGWCC 1 cut(s) 1193
SmiMI CAYNNNNRTG 3 cut(s) 408, 656, 1029
SmlI CTYRAG 1 cut(s) 823
SmoI CTYRAG 1 cut(s) 823
Sse9I AATT 3 cut(s) 516, 530, 813
SsiI CCGC 2 cut(s) 250, 1021
SspMI CTAG 3 cut(s) 728, 801, 1203
StyD4I CCNGG 2 cut(s) 618, 1273
TaaI ACNGT 2 cut(s) 96, 161
TaiI ACGT 2 cut(s) 195, 1169
TaqI TCGA 2 cut(s) 258, 888
TasI AATT 3 cut(s) 516, 530, 813
TatI WGTACW 3 cut(s) 879, 1161, 1257
TfiI GAWTC 6 cut(s) 14, 362, 566, 796, 1108, 1150
Tru1I TTAA 4 cut(s) 630, 678, 816, 1170
Tru9I TTAA 4 cut(s) 630, 678, 816, 1170
TscAI CASTG 2 cut(s) 99, 1036
TseFI GTSAC 4 cut(s) 459, 544, 658, 974
TseI GCWGC 3 cut(s) 215, 559, 1270
Tsp45I GTSAC 4 cut(s) 459, 544, 658, 974
TspDTI ATGAA 9 cut(s) 78, 88, 370, 428, 543, 845, 1027, 1064, 1211
TspGWI ACGGA 2 cut(s) 42, 528
TspRI CASTG 2 cut(s) 99, 1036
VpaK11BI GGWCC 1 cut(s) 1193
VspI ATTAAT 1 cut(s) 816
XceI RCATGY 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 3 cut(s) 728, 801, 1203
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.