Rmu_sc0000981.1_g000004

protein At1g32220, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000981.1
OG0012254
Physical Location & Seq
Forward (+)
13537 .. 24310
10774 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 900 bp
atgtcgactgtaaggctcgggcttgggttggttttggatgctggttcgattgctggtgatggttttgctaagtggtggtcagaaatggtgttttccggtgctggcagtgggaggagagagagagaggtaggggagaaagagagagaagggaggacgcgactgcaagggaggaaggagaaaaatgaggctaggtcctctaaattccgacgatcagaattttcaaatactggccaaaaccgaccattgaaagcagtaactgcaagctccggcaacacatttggcttaaccgaagagtttcggtctggtaggtcaaagttacatgattcatgggctaacaatgtgaactggtaccaaggaaaccttctatcacctgattcatggaaggatgctcttgacggtgtcacgtctgttatatcctgcgttggtggctttggctccaattcttacatgtataagattaatgggactgcaaacatcaatgcaattagagctgcttctgagcatggtgtgaaaagatttgtgtatgtctctgctgctgattttggcttggtaaattacttgctgcgaggatattatgagggaaagagagctgccgaaacagagatactaaccaaattcccttatggaggagtgattttgaggcctggatttatttacgggactcgaagtgttgggagcataaagttacctcttggtgtaatcggttctcctctggagatgttatttcaacaaacaagacctctgaaccagctgccactagtgggacctctctttactcctcctgtgaatgttactgctgtagcgaaggttgctgttagagcagctaccgatcccgtcttccctcctgggattgttgatatctacgggattcaacgttatactcagcataagtccaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

299

Amino Acids

32.69

Weight (kDa)

9.9

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 104 - 218 1.3e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012254)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10730
fragaria_vesca FvH4_2g41440 FvH4_2g41440
malus_domestica MD08G1049300.v1.1 MD15G1045600.v1.1
prunus_persica Prupe.1G399300_v2.0.a1
pyrus_communis pycom08g04120 pycom15g04250
rosa_chinensis RchiOBHm_Chr6g0299041
rosa_laevigata RLG00000011467
rosa_multiflora Rmu_sc0000981.1_g000004 Rmu_sc0032629.1_g000004
rosa_roxburghii Rroxscaffold_7G00169060
rosa_rugosa Rorug06G0284300
rosa_samantha Rh6AG395800 Rh6BG404400 Rh6CG410300 Rh6DG397000
rosa_wichuraiana Rw6G034640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 157
AclI AACGTT 1 cut(s) 874
AclWI GGATC 1 cut(s) 824
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 3 cut(s) 200, 215, 614
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 2 cut(s) 626, 761
AflIII ACRYGT 1 cut(s) 447
AgsI TTSAA 4 cut(s) 222, 247, 728, 872
AhlI ACTAGT 1 cut(s) 757
AjiI CACGTC 1 cut(s) 405
AjnI CCWGG 2 cut(s) 643, 844
AjuI GAANNNNNNNTTGG 2 cut(s) 345, 377
AluBI AGCT 5 cut(s) 264, 491, 590, 751, 824
AluI AGCT 5 cut(s) 264, 491, 590, 751, 824
Alw26I GTCTC 1 cut(s) 532
AlwI GGATC 1 cut(s) 824
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 17
AoxI GGCC 2 cut(s) 229, 641
ApeKI GCWGC 6 cut(s) 491, 533, 562, 590, 751, 821
ApoI RAATTY 3 cut(s) 200, 215, 614
AseI ATTAAT 1 cut(s) 459
Asp700I GAANNNNTTC 1 cut(s) 294
Asp718I GGTACC 1 cut(s) 348
AspS9I GGNCC 2 cut(s) 192, 764
AsuHPI GGTGA 2 cut(s) 68, 360
AvaI CYCGRG 1 cut(s) 17
AvaII GGWCC 2 cut(s) 192, 764
BalI TGGCCA 1 cut(s) 231
BanI GGYRCC 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 829
BbvI GCAGC 6 cut(s) 478, 520, 549, 577, 738, 833
BccI CCATC 1 cut(s) 53
BciT130I CCWGG 2 cut(s) 645, 846
BcoDI GTCTC 1 cut(s) 532
BcuI ACTAGT 1 cut(s) 757
BfaI CTAG 2 cut(s) 189, 758
BfmI CTRYAG 1 cut(s) 798
BisI GCNGC 6 cut(s) 492, 534, 563, 591, 752, 822
BlsI GCNGC 6 cut(s) 493, 535, 564, 592, 753, 823
Bme1390I CCNGG 2 cut(s) 645, 846
Bme18I GGWCC 2 cut(s) 192, 764
BmeT110I CYCGRG 1 cut(s) 17
BmgBI CACGTC 1 cut(s) 405
BmgT120I GGNCC 2 cut(s) 192, 764
BmiI GGNNCC 3 cut(s) 350, 436, 765
BmrFI CCNGG 2 cut(s) 645, 846
BmsI GCATC 2 cut(s) 28, 376
BpiI GAAGAC 1 cut(s) 829
BpmI CTGGAG 1 cut(s) 734
BsaJI CCNNGG 2 cut(s) 352, 845
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 2 cut(s) 626, 761
Bse1I ACTGG 2 cut(s) 232, 350
BseBI CCWGG 2 cut(s) 645, 846
BseDI CCNNGG 2 cut(s) 352, 845
BseGI GGATG 2 cut(s) 43, 391
BseLI CCNNNNNNNGG 2 cut(s) 626, 761
BseMII CTCAG 2 cut(s) 489, 896
BseNI ACTGG 2 cut(s) 232, 350
BseRI GAGGAG 4 cut(s) 127, 642, 699, 768
BseXI GCAGC 6 cut(s) 478, 520, 549, 577, 738, 833
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 2 cut(s) 231, 643
BshNI GGYRCC 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 17
BsiSI CCGG 2 cut(s) 96, 267
BslFI GGGAC 3 cut(s) 478, 673, 777
BslI CCNNNNNNNGG 2 cut(s) 626, 761
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 3 cut(s) 478, 673, 777
BsnI GGCC 2 cut(s) 231, 643
BsoBI CYCGRG 1 cut(s) 17
Bsp143I GATC 2 cut(s) 209, 829
BspANI GGCC 2 cut(s) 231, 643
BspCNI CTCAG 2 cut(s) 490, 895
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 350, 436, 765
BspPI GGATC 1 cut(s) 824
BspT107I GGYRCC 1 cut(s) 348
BsrI ACTGG 2 cut(s) 232, 350
BssECI CCNNGG 2 cut(s) 352, 845
BssMI GATC 2 cut(s) 209, 829
BssT1I CCWWGG 1 cut(s) 352
Bst2UI CCWGG 2 cut(s) 645, 846
Bst4CI ACNGT 2 cut(s) 10, 398
Bst6I CTCTTC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 103, 262
BstDEI CTNAG 3 cut(s) 69, 498, 882
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 2 cut(s) 43, 391
BstFNI CGCG 1 cut(s) 157
BstKTI GATC 2 cut(s) 212, 832
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 2 cut(s) 209, 829
BstMWI GCNNNNNNNGC 5 cut(s) 257, 426, 488, 809, 818
BstNI CCWGG 2 cut(s) 645, 846
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 2 cut(s) 643, 844
BstSFI CTRYAG 1 cut(s) 798
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 6 cut(s) 478, 520, 549, 577, 738, 833
BstV2I GAAGAC 1 cut(s) 829
BsuRI GGCC 2 cut(s) 231, 643
BtrI CACGTC 1 cut(s) 405
BtsCI GGATG 2 cut(s) 43, 391
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 2 cut(s) 103, 262
CaiI CAGNNNCTG 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 192, 764
CseI GACGC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 349
CviAII CATG 5 cut(s) 320, 327, 378, 448, 503
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 3 cut(s) 69, 498, 882
DpnI GATC 2 cut(s) 211, 831
DpnII GATC 2 cut(s) 209, 829
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 352
Eco147I AGGCCT 1 cut(s) 643
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 2 cut(s) 192, 764
Eco88I CYCGRG 1 cut(s) 17
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoO109I RGGNCCY 2 cut(s) 192, 764
EcoRII CCWGG 2 cut(s) 643, 844
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 5 cut(s) 323, 330, 381, 451, 506
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FaqI GGGAC 3 cut(s) 478, 673, 777
FatI CATG 5 cut(s) 319, 326, 377, 447, 502
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 492, 534, 563, 591, 752, 822
FokI GGATG 2 cut(s) 50, 398
Fsp4HI GCNGC 6 cut(s) 492, 534, 563, 591, 752, 822
FspBI CTAG 2 cut(s) 189, 758
GluI GCNGC 6 cut(s) 492, 534, 563, 591, 752, 822
GsuI CTGGAG 1 cut(s) 734
HaeIII GGCC 2 cut(s) 231, 643
HapII CCGG 2 cut(s) 96, 267
HgaI GACGC 1 cut(s) 163
Hin1II CATG 5 cut(s) 323, 330, 381, 451, 506
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 323, 374, 661, 868
HpaII CCGG 2 cut(s) 96, 267
HphI GGTGA 2 cut(s) 68, 360
Hpy166II GTNNAC 2 cut(s) 6, 343
Hpy188I TCNGA 5 cut(s) 82, 206, 214, 499, 744
Hpy188III TCNNGA 2 cut(s) 392, 713
Hpy8I GTNNAC 2 cut(s) 6, 343
Hpy99I CGWCG 1 cut(s) 210
HpyAV CCTTC 5 cut(s) 140, 166, 371, 376, 799
HpyCH4III ACNGT 2 cut(s) 10, 398
HpyCH4IV ACGT 2 cut(s) 404, 874
HpyCH4V TGCA 4 cut(s) 163, 260, 470, 482
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 426, 488, 809, 818
HpyF3I CTNAG 3 cut(s) 69, 498, 882
HpySE526I ACGT 2 cut(s) 404, 874
Hsp92II CATG 5 cut(s) 323, 330, 381, 451, 506
KpnI GGTACC 1 cut(s) 352
Kzo9I GATC 2 cut(s) 209, 829
LmnI GCTCC 3 cut(s) 269, 440, 675
Lsp1109I GCAGC 6 cut(s) 478, 520, 549, 577, 738, 833
LweI GCATC 2 cut(s) 28, 376
MaeI CTAG 2 cut(s) 189, 758
MaeII ACGT 2 cut(s) 404, 874
MaeIII GTNAC 5 cut(s) 253, 315, 400, 684, 790
MalI GATC 2 cut(s) 211, 831
MboI GATC 2 cut(s) 209, 829
MboII GAAGA 2 cut(s) 302, 829
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 6 cut(s) 200, 215, 439, 483, 553, 614
MluNI TGGCCA 1 cut(s) 231
MlyI GAGTC 1 cut(s) 655
MmeI TCCRAC 1 cut(s) 229
Mox20I TGGCCA 1 cut(s) 231
MroXI GAANNNNTTC 1 cut(s) 294
MscI TGGCCA 1 cut(s) 231
MseI TTAA 2 cut(s) 284, 459
Msp20I TGGCCA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 2 cut(s) 96, 267
MspR9I CCNGG 2 cut(s) 645, 846
MvaI CCWGG 2 cut(s) 645, 846
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 5 cut(s) 257, 426, 488, 809, 818
NdeII GATC 2 cut(s) 209, 829
NlaIII CATG 5 cut(s) 323, 330, 381, 451, 506
NlaIV GGNNCC 3 cut(s) 350, 436, 765
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 451
PceI AGGCCT 1 cut(s) 643
PciI ACATGT 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 3 cut(s) 323, 374, 868
PflFI GACNNNGTC 1 cut(s) 398
PkrI GCNGC 6 cut(s) 493, 535, 564, 592, 753, 823
PleI GAGTC 1 cut(s) 655
PpsI GAGTC 1 cut(s) 655
PpuMI RGGWCCY 2 cut(s) 192, 764
PscI ACATGT 1 cut(s) 447
PshBI ATTAAT 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 874
Psp5II RGGWCCY 2 cut(s) 192, 764
Psp6I CCWGG 2 cut(s) 643, 844
PspGI CCWGG 2 cut(s) 643, 844
PspN4I GGNNCC 3 cut(s) 350, 436, 765
PspPI GGNCC 2 cut(s) 192, 764
PspPPI RGGWCCY 2 cut(s) 192, 764
PstNI CAGNNNCTG 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 398
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 284, 459
SatI GCNGC 6 cut(s) 492, 534, 563, 591, 752, 822
Sau3AI GATC 2 cut(s) 209, 829
Sau96I GGNCC 2 cut(s) 192, 764
SchI GAGTC 1 cut(s) 655
ScrFI CCNGG 2 cut(s) 645, 846
SfaNI GCATC 2 cut(s) 28, 376
SfcI CTRYAG 1 cut(s) 798
SinI GGWCC 2 cut(s) 192, 764
SpeI ACTAGT 1 cut(s) 757
Sse9I AATT 6 cut(s) 200, 215, 439, 483, 553, 614
SseBI AGGCCT 1 cut(s) 643
SspMI CTAG 2 cut(s) 189, 758
StuI AGGCCT 1 cut(s) 643
StyD4I CCNGG 2 cut(s) 643, 844
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 2 cut(s) 10, 398
TaiI ACGT 2 cut(s) 407, 877
TaqI TCGA 3 cut(s) 5, 47, 664
TaqII GACCGA 1 cut(s) 288
TasI AATT 6 cut(s) 200, 215, 439, 483, 553, 614
TfiI GAWTC 3 cut(s) 323, 374, 868
Tru1I TTAA 2 cut(s) 284, 459
Tru9I TTAA 2 cut(s) 284, 459
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 6 cut(s) 491, 533, 562, 590, 751, 821
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 2 cut(s) 315, 366
TspRI CASTG 1 cut(s) 112
Tth111I GACNNNGTC 1 cut(s) 398
VpaK11BI GGWCC 2 cut(s) 192, 764
VspI ATTAAT 1 cut(s) 459
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 3 cut(s) 200, 215, 614
XceI RCATGY 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 2 cut(s) 189, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.